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The map label for this gene is tuf

Identifier: 85374468

GI number: 85374468

Start: 1686786

End: 1687976

Strand: Reverse

Name: tuf

Synonym: ELI_08205

Alternate gene names: 85374468

Gene position: 1687976-1686786 (Counterclockwise)

Preceding gene: 85374469

Following gene: 85374467

Centisome position: 55.3

GC content: 61.8

Gene sequence:

>1191_bases
ATGGCTAAGGAAAAATTCGAGCGCAACAAGCCGCACTGCAACGTCGGCACCATCGGTCACGTCGACCACGGCAAGACCAC
GCTGACCGCGGCGATCACGAAGGTGATGGCCGATGTGTACGGCGGTTCGGCTGTCGACTTCGCCAACATCGACAAGGCTC
CGGAAGAGCGCGAGCGCGGGATCACCATCTCGACCGCGCACGTCGAGTACGAAACCGACGCGCGCCACTACGCTCACGTC
GATTGCCCGGGGCACGCCGACTATGTGAAGAACATGATCACCGGTGCGGCCCAGATGGACGGCGCCATCCTGGTCGTGAA
CGCTGCCGACGGCCCGATGCCGCAGACCCGCGAGCACATCCTGCTCTCGCGTCAGGTCGGCGTTCCCGCGCTGGTCGTGT
ACCTCAACAAGGTCGACCAGGTCGATGACGAAGAGCTGCTCGAACTGGTCGAACTCGAAGTTCGCGAACTGCTCAGCGAG
TATGATTTCGACGGCGACAACATTCCGATCGTCAAGGGTTCGGCTCTGGCCGCTCTCGAAGGTCGCGACGACAACATCGG
CAAGGAATCGATCGTCGAGCTGATGAAGGCCGTCGATGAGCACATCCCGCAGCCGGAACGTCCGGTCGATCAGGACTTCC
TGATGCCGATCGAGGACGTGTTCTCGATCTCGGGTCGTGGTACGGTTGTGACCGGTCGTGTCGAAACCGGCGTTGTCAAT
GTGGGCGATGAAGTCGAAATCGTCGGCATCAAGGACACCACCAAGACGACCGTTACCGGCGTCGAAATGTTCCGTAAGCT
GCTCGATCGCGGTGAAGCCGGCGACAACATCGGTGCCCTGATCCGCGGCGTCGGCCGTGAAGACGTCGAGCGTGGCCAGG
TCCTCGCGAAGCCGGGTTCGGTTACGCCGCACACCGAGTTCAGCGCGGAAGTCTACGTCCTTTCGAAGGACGAAGGTGGC
CGTCACACGCCGTTCTTCGCCAACTACCGTCCGCAGTTCTACTTCCGCACGACCGACGTGACCGGTGAAGTGATCCTGCC
GGAAGGCACCGAGATGGTGATGCCGGGCGACAACGTGACGATCAACGTCAAGCTGATCGCTCCGATCGCCATGGACGAAG
GCCTCCGCTTCGCAATCCGCGAAGGCGGCCGCACCGTCGGCTCGGGCGTTGTTTCGAAGATCACCGTCTAA

Upstream 100 bases:

>100_bases
TTGCCTAAGGCGAGCAACAGGTCTAGGGGCGGCGCCTGATTCAGCGGGCGCCGCATCCTCTCCCGCAGGAATTCAATTTC
AAGCAGAGGTTATTTGAGAA

Downstream 100 bases:

>100_bases
GCCGGCGCCTGATCTGACGGCACGCTGCTAGCGTTAGAAACACCCTCTCCCGGCAACGGGAGGGGGTGTTTTCTTTTGCA
CGAGCGAGTTAGCTAGCGGC

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERNKPHCNVGTIGHVDHGKTTLTAAITKVMADVYGGSAVDFANIDKAPEERERGITISTAHVEYETDARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLSRQVGVPALVVYLNKVDQVDDEELLELVELEVRELLSE
YDFDGDNIPIVKGSALAALEGRDDNIGKESIVELMKAVDEHIPQPERPVDQDFLMPIEDVFSISGRGTVVTGRVETGVVN
VGDEVEIVGIKDTTKTTVTGVEMFRKLLDRGEAGDNIGALIRGVGREDVERGQVLAKPGSVTPHTEFSAEVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVTINVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITV

Sequences:

>Translated_396_residues
MAKEKFERNKPHCNVGTIGHVDHGKTTLTAAITKVMADVYGGSAVDFANIDKAPEERERGITISTAHVEYETDARHYAHV
DCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLSRQVGVPALVVYLNKVDQVDDEELLELVELEVRELLSE
YDFDGDNIPIVKGSALAALEGRDDNIGKESIVELMKAVDEHIPQPERPVDQDFLMPIEDVFSISGRGTVVTGRVETGVVN
VGDEVEIVGIKDTTKTTVTGVEMFRKLLDRGEAGDNIGALIRGVGREDVERGQVLAKPGSVTPHTEFSAEVYVLSKDEGG
RHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVTINVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITV
>Mature_395_residues
AKEKFERNKPHCNVGTIGHVDHGKTTLTAAITKVMADVYGGSAVDFANIDKAPEERERGITISTAHVEYETDARHYAHVD
CPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHILLSRQVGVPALVVYLNKVDQVDDEELLELVELEVRELLSEY
DFDGDNIPIVKGSALAALEGRDDNIGKESIVELMKAVDEHIPQPERPVDQDFLMPIEDVFSISGRGTVVTGRVETGVVNV
GDEVEIVGIKDTTKTTVTGVEMFRKLLDRGEAGDNIGALIRGVGREDVERGQVLAKPGSVTPHTEFSAEVYVLSKDEGGR
HTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVTINVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITV

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=57.0332480818414, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=30.6843267108168, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4503475, Length=349, Percent_Identity=32.9512893982808, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI223555963, Length=439, Percent_Identity=28.7015945330296, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI5729864, Length=439, Percent_Identity=28.7015945330296, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI54607086, Length=328, Percent_Identity=29.5731707317073, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194097354, Length=336, Percent_Identity=28.8690476190476, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI194018522, Length=337, Percent_Identity=28.7833827893175, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI194018520, Length=336, Percent_Identity=28.8690476190476, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=29.0123456790123, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=25.2631578947368, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI94966754, Length=136, Percent_Identity=32.3529411764706, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=394, Percent_Identity=74.8730964467005, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1789737, Length=394, Percent_Identity=74.8730964467005, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI2367247, Length=337, Percent_Identity=33.2344213649852, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI48994988, Length=288, Percent_Identity=30.9027777777778, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789108, Length=265, Percent_Identity=30.5660377358491, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788922, Length=145, Percent_Identity=31.7241379310345, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=398, Percent_Identity=51.2562814070352, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.4108527131783, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17552884, Length=448, Percent_Identity=30.3571428571429, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17569207, Length=448, Percent_Identity=30.3571428571429, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI32566303, Length=429, Percent_Identity=28.6713286713287, Blast_Score=137, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=28.0182232346241, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=28.0182232346241, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=29.8295454545455, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=31.5018315018315, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.961038961039, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=233, Percent_Identity=27.0386266094421, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556745, Length=135, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=394, Percent_Identity=64.9746192893401, Blast_Score=525, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=30.4545454545455, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=30.4545454545455, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=448, Percent_Identity=25.8928571428571, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6320377, Length=363, Percent_Identity=26.9972451790634, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=289, Percent_Identity=27.3356401384083, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=152, Percent_Identity=30.9210526315789, Blast_Score=67, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=132, Percent_Identity=34.0909090909091, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=58.4615384615385, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=58.4615384615385, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=46.6321243523316, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=30.7692307692308, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=30.7692307692308, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI45553807, Length=456, Percent_Identity=30.2631578947368, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI45553816, Length=456, Percent_Identity=30.2631578947368, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI24651721, Length=456, Percent_Identity=30.2631578947368, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI17864154, Length=456, Percent_Identity=30.2631578947368, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.3531353135314, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI45550900, Length=444, Percent_Identity=27.2522522522523, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137380, Length=346, Percent_Identity=29.7687861271676, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24647046, Length=371, Percent_Identity=26.4150943396226, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647048, Length=371, Percent_Identity=26.4150943396226, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28572034, Length=175, Percent_Identity=37.1428571428571, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_ERYLH (Q2N9A8)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458530.1
- HSSP:   P0A6N1
- ProteinModelPortal:   Q2N9A8
- SMR:   Q2N9A8
- STRING:   Q2N9A8
- GeneID:   3871601
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_08205
- NMPDR:   fig|314225.3.peg.1401
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   ERKPHIN
- PhylomeDB:   Q2N9A8
- ProtClustDB:   PRK00049
- BioCyc:   ELIT314225:ELI_08205-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 43064; Mature: 42933

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERNKPHCNVGTIGHVDHGKTTLTAAITKVMADVYGGSAVDFANIDKAPEERERG
CCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHCC
ITISTAHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPALVVYLNKVDQVDDEELLELVELEVRELLSEYDFDGDNIPIVKGSALAALE
EEECCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEC
GRDDNIGKESIVELMKAVDEHIPQPERPVDQDFLMPIEDVFSISGRGTVVTGRVETGVVN
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHCCCCCCEEEEEEEEECEEE
VGDEVEIVGIKDTTKTTVTGVEMFRKLLDRGEAGDNIGALIRGVGREDVERGQVLAKPGS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCEEEECCCC
VTPHTEFSAEVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVT
CCCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCEEEECCCCEE
INVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITV
EEEEEEEEEECCCCCEEEECCCCCCHHCCCHHEECC
>Mature Secondary Structure 
AKEKFERNKPHCNVGTIGHVDHGKTTLTAAITKVMADVYGGSAVDFANIDKAPEERERG
CCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHCC
ITISTAHVEYETDARHYAHVDCPGHADYVKNMITGAAQMDGAILVVNAADGPMPQTREHI
CEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPALVVYLNKVDQVDDEELLELVELEVRELLSEYDFDGDNIPIVKGSALAALE
EEECCCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEEC
GRDDNIGKESIVELMKAVDEHIPQPERPVDQDFLMPIEDVFSISGRGTVVTGRVETGVVN
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHCCCCCCEEEEEEEEECEEE
VGDEVEIVGIKDTTKTTVTGVEMFRKLLDRGEAGDNIGALIRGVGREDVERGQVLAKPGS
CCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHCCEEEECCCC
VTPHTEFSAEVYVLSKDEGGRHTPFFANYRPQFYFRTTDVTGEVILPEGTEMVMPGDNVT
CCCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEEEECCCCEEECCCCCEEEECCCCEE
INVKLIAPIAMDEGLRFAIREGGRTVGSGVVSKITV
EEEEEEEEEECCCCCEEEECCCCCCHHCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA