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The map label for this gene is gatA [H]

Identifier: 85374431

GI number: 85374431

Start: 1662936

End: 1664270

Strand: Direct

Name: gatA [H]

Synonym: ELI_08020

Alternate gene names: 85374431

Gene position: 1662936-1664270 (Clockwise)

Preceding gene: 85374430

Following gene: 85374432

Centisome position: 54.48

GC content: 66.22

Gene sequence:

>1335_bases
ATGGCCTATCCGACATTGACCGACGAACCGGGCGGGATCGAAACCGCCGCGCAGATCCGGGACGGACATATTTCGCCGCT
CGAGGCGGTCGAAGCGGCAATCGGCAGGATCGAAAAGCTCGATGAGGCGATCAATGCCGTCGTGATCCGCGATTTCGACC
GCGCGCGCGAGACCGCCAGGGCGATGGATGGCATGGAGATCATGCCGCACCAGCCGCTGTTCGGCGTGCCGATGACGATC
AAGGAAAGCTTCGCCATTGCCGGCCTGCCGAGTTGCTGGGGGTTCAAGGAGTTCGAAGGGCAGGTCCAGGACGCGGATTC
GACCGTCGTCCGGCAACTGAAGGCAGCAGGGGCGATCCTGCTCGGCAAGACCAATATCCCCCCTGCCCTCGCCGACTGGC
AGAGCGCCAACCCGGTCTATGGCCGCACCGGCAATCCGCATGACACGACGCGCAGCCCCGGCGGCTCGTCCGGCGGCTCC
GCCGCGGCGGTCGCGAGCGGGATGGTGCCGTTGGAGTATGGCACCGACATTGGCGGCTCGGTCCGCGTGCCGGCGCATTT
CTGCGGCACCTGGGGCCACAAGACGAGCTGGGGCCTCGTCTCCAAGCAAGGTCACGATCATCCGGCCTTCAAGGGCATGG
ATGCGCATGACGGCGCGCTGAGCATCGCCGGGCCGCTGACCCGCAATGCCGACGACCTCGCGCTGATGCTGGAACTGACG
GCCACCTTGCCACTGCGCCGCCGCGAGAAGGACCTGAAAGCCTGCCGCTTCCTGTTGCTGCTCGATCACCCGGTCAGCGA
GGTCGACGCCAGCGTGCGCGGGCCGATCGAAACTGCAGCGGAAGCGCTGCGCAAGGCAGGCGTGCATGTCGACACGTCAG
CGCCGGAGATGCCCGATCTCGAAGGCGATCATGCCGATTATATGCGCATGCTCAACATCGCGATGGCGCGCGGAGCCCCG
GCAGACGATGGCACGCGCGCGACGGCCACCGACTGGTTCGACCTGCTCGATGCCCAGACGCGCTGCGAACTTTCCTGGGC
AAACCTTTTCGACAGCTACGATTTCATCCTCGCGCCGCCGGCGCCGATCCTCGCGATGAAGCATGTCGAAGGTCAGTATT
TCCAGGGCGAGACCATCATCAACGGCAACACCCATGCCGCGGCGGACGGGCTTGCCTGGGCTGGTATCGCCACCTTCCCC
AACCTGCCCTCCACCGTGCTGCCCGTCGGCGGCACCGGCGACCTCCCGTGCGGGATGCAGGTGATAGGGCCGCGCTGGTC
GGACTTCGATTGCATCGCAGCAGCCAAGGCCATAGGGGCGCTGCTGCACGACTGA

Upstream 100 bases:

>100_bases
AGATCAAGGCGATGGCGAACCCGGAAAACCTGTTCGCCAAATAGACTTTTGCGCATCGTCCCGCCGCGTGGCAGAACGCC
GTGCGAGAGGGGAAATACGC

Downstream 100 bases:

>100_bases
AGCGGCAAGCAGGGACGACATTATGGCGCGGACGCCTATCATGCAGCGCTTTGCAAAATGGCATATCTGGCTCGGCTGGC
TGGTCGGCGTTCCGATCCTG

Product: putative amidase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAYPTLTDEPGGIETAAQIRDGHISPLEAVEAAIGRIEKLDEAINAVVIRDFDRARETARAMDGMEIMPHQPLFGVPMTI
KESFAIAGLPSCWGFKEFEGQVQDADSTVVRQLKAAGAILLGKTNIPPALADWQSANPVYGRTGNPHDTTRSPGGSSGGS
AAAVASGMVPLEYGTDIGGSVRVPAHFCGTWGHKTSWGLVSKQGHDHPAFKGMDAHDGALSIAGPLTRNADDLALMLELT
ATLPLRRREKDLKACRFLLLLDHPVSEVDASVRGPIETAAEALRKAGVHVDTSAPEMPDLEGDHADYMRMLNIAMARGAP
ADDGTRATATDWFDLLDAQTRCELSWANLFDSYDFILAPPAPILAMKHVEGQYFQGETIINGNTHAAADGLAWAGIATFP
NLPSTVLPVGGTGDLPCGMQVIGPRWSDFDCIAAAKAIGALLHD

Sequences:

>Translated_444_residues
MAYPTLTDEPGGIETAAQIRDGHISPLEAVEAAIGRIEKLDEAINAVVIRDFDRARETARAMDGMEIMPHQPLFGVPMTI
KESFAIAGLPSCWGFKEFEGQVQDADSTVVRQLKAAGAILLGKTNIPPALADWQSANPVYGRTGNPHDTTRSPGGSSGGS
AAAVASGMVPLEYGTDIGGSVRVPAHFCGTWGHKTSWGLVSKQGHDHPAFKGMDAHDGALSIAGPLTRNADDLALMLELT
ATLPLRRREKDLKACRFLLLLDHPVSEVDASVRGPIETAAEALRKAGVHVDTSAPEMPDLEGDHADYMRMLNIAMARGAP
ADDGTRATATDWFDLLDAQTRCELSWANLFDSYDFILAPPAPILAMKHVEGQYFQGETIINGNTHAAADGLAWAGIATFP
NLPSTVLPVGGTGDLPCGMQVIGPRWSDFDCIAAAKAIGALLHD
>Mature_443_residues
AYPTLTDEPGGIETAAQIRDGHISPLEAVEAAIGRIEKLDEAINAVVIRDFDRARETARAMDGMEIMPHQPLFGVPMTIK
ESFAIAGLPSCWGFKEFEGQVQDADSTVVRQLKAAGAILLGKTNIPPALADWQSANPVYGRTGNPHDTTRSPGGSSGGSA
AAVASGMVPLEYGTDIGGSVRVPAHFCGTWGHKTSWGLVSKQGHDHPAFKGMDAHDGALSIAGPLTRNADDLALMLELTA
TLPLRRREKDLKACRFLLLLDHPVSEVDASVRGPIETAAEALRKAGVHVDTSAPEMPDLEGDHADYMRMLNIAMARGAPA
DDGTRATATDWFDLLDAQTRCELSWANLFDSYDFILAPPAPILAMKHVEGQYFQGETIINGNTHAAADGLAWAGIATFPN
LPSTVLPVGGTGDLPCGMQVIGPRWSDFDCIAAAKAIGALLHD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=253, Percent_Identity=35.5731225296443, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI166795287, Length=217, Percent_Identity=33.6405529953917, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI222831590, Length=249, Percent_Identity=27.710843373494, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17537465, Length=486, Percent_Identity=27.9835390946502, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI71990152, Length=249, Percent_Identity=36.144578313253, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17538252, Length=213, Percent_Identity=36.150234741784, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=245, Percent_Identity=31.8367346938775, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538254, Length=210, Percent_Identity=34.2857142857143, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=162, Percent_Identity=36.4197530864198, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556278, Length=162, Percent_Identity=36.4197530864198, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=163, Percent_Identity=31.2883435582822, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=225, Percent_Identity=28, Blast_Score=77, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=144, Percent_Identity=39.5833333333333, Blast_Score=70, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550774, Length=240, Percent_Identity=39.1666666666667, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24648435, Length=239, Percent_Identity=39.3305439330544, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24648437, Length=239, Percent_Identity=39.3305439330544, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI24644968, Length=487, Percent_Identity=26.8993839835729, Blast_Score=148, Evalue=6e-36,
Organism=Drosophila melanogaster, GI21356731, Length=479, Percent_Identity=27.7661795407098, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24652985, Length=475, Percent_Identity=27.5789473684211, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI19922090, Length=475, Percent_Identity=27.5789473684211, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24652981, Length=475, Percent_Identity=27.5789473684211, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24652983, Length=475, Percent_Identity=27.5789473684211, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24648441, Length=171, Percent_Identity=42.6900584795322, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24648439, Length=171, Percent_Identity=42.6900584795322, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161078093, Length=239, Percent_Identity=33.8912133891213, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24648113, Length=236, Percent_Identity=32.2033898305085, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 47144; Mature: 47013

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYPTLTDEPGGIETAAQIRDGHISPLEAVEAAIGRIEKLDEAINAVVIRDFDRARETAR
CCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHH
AMDGMEIMPHQPLFGVPMTIKESFAIAGLPSCWGFKEFEGQVQDADSTVVRQLKAAGAIL
HHCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCEEE
LGKTNIPPALADWQSANPVYGRTGNPHDTTRSPGGSSGGSAAAVASGMVPLEYGTDIGGS
EECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCCCCCCC
VRVPAHFCGTWGHKTSWGLVSKQGHDHPAFKGMDAHDGALSIAGPLTRNADDLALMLELT
EECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHEEEEHHH
ATLPLRRREKDLKACRFLLLLDHPVSEVDASVRGPIETAAEALRKAGVHVDTSAPEMPDL
HCCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHCCCHHHHHHHHHHCCCEEECCCCCCCCC
EGDHADYMRMLNIAMARGAPADDGTRATATDWFDLLDAQTRCELSWANLFDSYDFILAPP
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHEEHHHHHHCCCCEEECCC
APILAMKHVEGQYFQGETIINGNTHAAADGLAWAGIATFPNLPSTVLPVGGTGDLPCGMQ
CHHEEHHHCCCCEECCCEEECCCCCCCCCCCEECCHHHCCCCCCCEEECCCCCCCCCCCE
VIGPRWSDFDCIAAAKAIGALLHD
EECCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYPTLTDEPGGIETAAQIRDGHISPLEAVEAAIGRIEKLDEAINAVVIRDFDRARETAR
CCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHH
AMDGMEIMPHQPLFGVPMTIKESFAIAGLPSCWGFKEFEGQVQDADSTVVRQLKAAGAIL
HHCCCCCCCCCCCCCCCHHHHCCEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCEEE
LGKTNIPPALADWQSANPVYGRTGNPHDTTRSPGGSSGGSAAAVASGMVPLEYGTDIGGS
EECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCEEECCCCCCCCC
VRVPAHFCGTWGHKTSWGLVSKQGHDHPAFKGMDAHDGALSIAGPLTRNADDLALMLELT
EECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHEEEEHHH
ATLPLRRREKDLKACRFLLLLDHPVSEVDASVRGPIETAAEALRKAGVHVDTSAPEMPDL
HCCCCHHHHHHHHHHHHHHHCCCCHHHHCCHHCCCHHHHHHHHHHCCCEEECCCCCCCCC
EGDHADYMRMLNIAMARGAPADDGTRATATDWFDLLDAQTRCELSWANLFDSYDFILAPP
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHEEHHHHHHCCCCEEECCC
APILAMKHVEGQYFQGETIINGNTHAAADGLAWAGIATFPNLPSTVLPVGGTGDLPCGMQ
CHHEEHHHCCCCEECCCEEECCCCCCCCCCCEECCHHHCCCCCCCEEECCCCCCCCCCCE
VIGPRWSDFDCIAAAKAIGALLHD
EECCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA