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The map label for this gene is btuB [H]

Identifier: 85374419

GI number: 85374419

Start: 1647564

End: 1649405

Strand: Reverse

Name: btuB [H]

Synonym: ELI_07960

Alternate gene names: 85374419

Gene position: 1649405-1647564 (Counterclockwise)

Preceding gene: 85374435

Following gene: 85374418

Centisome position: 54.04

GC content: 65.09

Gene sequence:

>1842_bases
ATGTATCAGAAGTCAGATGCGCCGAGAACCATTACCGTGACCGGAACGCCGATCGAGACCGAAGACACCGGCCAGCCGGT
CACCATCATTACTCGCGAAGAAATCGAGCAGGTCCAGGGTCCGGACCTCGCCCGCATCCTGCGCCGCGCGCCGGGTGTCA
CGATCAGCCGCAATGGCGGCGTGGGCGGTTTTACCGGTGTGCGCGTGCGCGGGGCTGAGGCGGAGCAACTGCTCGTGCTG
ATCGACGGTGTACGCGTCAACGATCCGTCCTCGCCCGCGGGCGGCTTCGACTTCGGCAATCTCCTGCCCGGCAATATCGA
CCAGATCGACCTGCTGCGCGGGTCGAATTCCACCGTCTGGGGCAGCGATGCGATCGGCGGTGTGCTGGCGGTGAAGACCC
GCTCCGAAAGCGGCATCGAAGGCAGCGCCGAATACGGCGCGCGCGACAGCTTCAACGCCAATCTCGCAGGCAGCATCGAA
GGCGAGGATGCCTTCCTAAGCCTTGCGGGCGGCTATTACACCAGCGACGGCTTCTCCGCCGCCGCCAGCGGCACGGAGCC
GGACGGTTTCGAGCAGTTCGCACTGACTGCGCGCGGGCGCGTGTTTCTGAGCGCGACGACCGAAGTCTTCGCCCGCGGTC
GCTATGCCGAGGGCGAGCTTGAGATCGACGGTTTCCCGTTCCCGACCTTCACGCTCTCCGACACGCCGGAGTTTCAGGAA
ACGACCCAGTATTCCGGCGCGTTCGGCCTGCTGCACGACAACGGCCCGCTTTACTTGCAGGCGGCCTACAGCTTCGCCGA
AACGGAACGCGCCAATTTCGATCCCACCTTCGGGACGGCACCGGGCTTCACCAGCGACGGCCACTCCGACCGGATCGACG
TGAAGGGCGAATACCGCCTGATCGGCCCGCTGTTGGTCAATTTCGGGGCCGATGCGGAATGGACCAGCCTCGAAACCAAT
TTCACGCCGAGGCAGGATGTGCGCATCCTGGGCGGCTATGTTCAGGCGGGCATCGAATTCGGCGGGCTTTCGGGCCATAT
CGGATTGCGGCAGGACGATCACCAGCGCTTCGGCGGGGCGACCAGTTTCGGGGCCGATGTCAGTTACGAGGTCGCCCCCG
ATGTGCGGGTTAAGGCGAGCGTCGGCGAGGGCTTCAAGGCGCCGAGCCTGTTCCAGCTGTTCAGCGACTTCGGCAACGAG
CTTCTGCGCCCGGAGACCAGCACCAGCTTCGATTTGGGCCTTGCGTTCAGGGATCGCAGCGCGCCGGCCTACGCAGGCAT
CACGCTGTTCCGCCGCGACAGCGAGGACCTGATCCAGTTCGTCAGTTGCTTCGGCGTCGTCGGCGGTATCTGCACCGGGC
GACCCTTCGGCACCTACGACAACGTCGCCCGCGCGCGGGCGCAAGGTGTCGAGATGGAAGCCGGGTTCACGATATCCGAC
GCTCTGCGCGGGCGGGCGACCTACAGCTTCATCGATACCGAAGATCGCAGCGCGGGAGCGTTGACACAAGGCAATCGGCT
CGCCCGCCGTCCGCGCAATGCCGCGAGTGCTTCGCTCGACTGGGACAGCGCGCGGGGCTTGGAATCGCTGCTGCGCCCGA
AGATCGGCGCGGACCTGCGCTATGTATCGGGCGCGTTCGACGATGCTTTCAATACGGTGCCGCTTGAAAGCTACGTCGTG
GTCGATCTGCGCGCGGAATTGTCGGTGGCGCAATTGAATGGCGATCATCCGCTGGTGCTGTTCGCCCGCGTCGAGAACGT
GTTCGCCGAGGACTACCAGACCGCCGCCGGATATGCGCAGGCCGGGCGCGGCGTGTTCGCAGGCGCGAGGTTCAGTTTTT
GA

Upstream 100 bases:

>100_bases
CCCGGACCTAGAGGAGCCGAATAAAGAGTGTGATGAACCGCCCCAGTTCGTCGGCACCGGGCATCCTGTTCGATGTGGCA
GCGAAGATGTTTCAGTCTGG

Downstream 100 bases:

>100_bases
GCCGAGCTTTCCGCCCGTCATCTTCGCGAATGCTGAGGCCCGGACTTGCAGGCATCGCCAGATCGCCCTGGGTTTCCGCC
CTCGCCGGAATGGCGCTCTT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MYQKSDAPRTITVTGTPIETEDTGQPVTIITREEIEQVQGPDLARILRRAPGVTISRNGGVGGFTGVRVRGAEAEQLLVL
IDGVRVNDPSSPAGGFDFGNLLPGNIDQIDLLRGSNSTVWGSDAIGGVLAVKTRSESGIEGSAEYGARDSFNANLAGSIE
GEDAFLSLAGGYYTSDGFSAAASGTEPDGFEQFALTARGRVFLSATTEVFARGRYAEGELEIDGFPFPTFTLSDTPEFQE
TTQYSGAFGLLHDNGPLYLQAAYSFAETERANFDPTFGTAPGFTSDGHSDRIDVKGEYRLIGPLLVNFGADAEWTSLETN
FTPRQDVRILGGYVQAGIEFGGLSGHIGLRQDDHQRFGGATSFGADVSYEVAPDVRVKASVGEGFKAPSLFQLFSDFGNE
LLRPETSTSFDLGLAFRDRSAPAYAGITLFRRDSEDLIQFVSCFGVVGGICTGRPFGTYDNVARARAQGVEMEAGFTISD
ALRGRATYSFIDTEDRSAGALTQGNRLARRPRNAASASLDWDSARGLESLLRPKIGADLRYVSGAFDDAFNTVPLESYVV
VDLRAELSVAQLNGDHPLVLFARVENVFAEDYQTAAGYAQAGRGVFAGARFSF

Sequences:

>Translated_613_residues
MYQKSDAPRTITVTGTPIETEDTGQPVTIITREEIEQVQGPDLARILRRAPGVTISRNGGVGGFTGVRVRGAEAEQLLVL
IDGVRVNDPSSPAGGFDFGNLLPGNIDQIDLLRGSNSTVWGSDAIGGVLAVKTRSESGIEGSAEYGARDSFNANLAGSIE
GEDAFLSLAGGYYTSDGFSAAASGTEPDGFEQFALTARGRVFLSATTEVFARGRYAEGELEIDGFPFPTFTLSDTPEFQE
TTQYSGAFGLLHDNGPLYLQAAYSFAETERANFDPTFGTAPGFTSDGHSDRIDVKGEYRLIGPLLVNFGADAEWTSLETN
FTPRQDVRILGGYVQAGIEFGGLSGHIGLRQDDHQRFGGATSFGADVSYEVAPDVRVKASVGEGFKAPSLFQLFSDFGNE
LLRPETSTSFDLGLAFRDRSAPAYAGITLFRRDSEDLIQFVSCFGVVGGICTGRPFGTYDNVARARAQGVEMEAGFTISD
ALRGRATYSFIDTEDRSAGALTQGNRLARRPRNAASASLDWDSARGLESLLRPKIGADLRYVSGAFDDAFNTVPLESYVV
VDLRAELSVAQLNGDHPLVLFARVENVFAEDYQTAAGYAQAGRGVFAGARFSF
>Mature_613_residues
MYQKSDAPRTITVTGTPIETEDTGQPVTIITREEIEQVQGPDLARILRRAPGVTISRNGGVGGFTGVRVRGAEAEQLLVL
IDGVRVNDPSSPAGGFDFGNLLPGNIDQIDLLRGSNSTVWGSDAIGGVLAVKTRSESGIEGSAEYGARDSFNANLAGSIE
GEDAFLSLAGGYYTSDGFSAAASGTEPDGFEQFALTARGRVFLSATTEVFARGRYAEGELEIDGFPFPTFTLSDTPEFQE
TTQYSGAFGLLHDNGPLYLQAAYSFAETERANFDPTFGTAPGFTSDGHSDRIDVKGEYRLIGPLLVNFGADAEWTSLETN
FTPRQDVRILGGYVQAGIEFGGLSGHIGLRQDDHQRFGGATSFGADVSYEVAPDVRVKASVGEGFKAPSLFQLFSDFGNE
LLRPETSTSFDLGLAFRDRSAPAYAGITLFRRDSEDLIQFVSCFGVVGGICTGRPFGTYDNVARARAQGVEMEAGFTISD
ALRGRATYSFIDTEDRSAGALTQGNRLARRPRNAASASLDWDSARGLESLLRPKIGADLRYVSGAFDDAFNTVPLESYVV
VDLRAELSVAQLNGDHPLVLFARVENVFAEDYQTAAGYAQAGRGVFAGARFSF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=639, Percent_Identity=27.5430359937402, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788478, Length=210, Percent_Identity=31.4285714285714, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786798, Length=200, Percent_Identity=25.5, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 65592; Mature: 65592

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS01156 TONB_DEPENDENT_REC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQKSDAPRTITVTGTPIETEDTGQPVTIITREEIEQVQGPDLARILRRAPGVTISRNGG
CCCCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHCCCCEEECCCC
VGGFTGVRVRGAEAEQLLVLIDGVRVNDPSSPAGGFDFGNLLPGNIDQIDLLRGSNSTVW
CCCCCCEEEECCCCCEEEEEEECEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
GSDAIGGVLAVKTRSESGIEGSAEYGARDSFNANLAGSIEGEDAFLSLAGGYYTSDGFSA
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCEEECCCCCC
AASGTEPDGFEQFALTARGRVFLSATTEVFARGRYAEGELEIDGFPFPTFTLSDTPEFQE
CCCCCCCCCHHHEEEEECCEEEEEEHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCHHH
TTQYSGAFGLLHDNGPLYLQAAYSFAETERANFDPTFGTAPGFTSDGHSDRIDVKGEYRL
HHCCCCEEEEEECCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEE
IGPLLVNFGADAEWTSLETNFTPRQDVRILGGYVQAGIEFGGLSGHIGLRQDDHQRFGGA
EEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHCCCC
TSFGADVSYEVAPDVRVKASVGEGFKAPSLFQLFSDFGNELLRPETSTSFDLGLAFRDRS
CCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
APAYAGITLFRRDSEDLIQFVSCFGVVGGICTGRPFGTYDNVARARAQGVEMEAGFTISD
CCCEECEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCHHH
ALRGRATYSFIDTEDRSAGALTQGNRLARRPRNAASASLDWDSARGLESLLRPKIGADLR
HHCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
YVSGAFDDAFNTVPLESYVVVDLRAELSVAQLNGDHPLVLFARVENVFAEDYQTAAGYAQ
EECCCHHHCCCCCCCCEEEEEEEECCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHH
AGRGVFAGARFSF
CCCCEEECCCCCC
>Mature Secondary Structure
MYQKSDAPRTITVTGTPIETEDTGQPVTIITREEIEQVQGPDLARILRRAPGVTISRNGG
CCCCCCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHCCCCHHHHHHHCCCCEEECCCC
VGGFTGVRVRGAEAEQLLVLIDGVRVNDPSSPAGGFDFGNLLPGNIDQIDLLRGSNSTVW
CCCCCCEEEECCCCCEEEEEEECEEECCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEE
GSDAIGGVLAVKTRSESGIEGSAEYGARDSFNANLAGSIEGEDAFLSLAGGYYTSDGFSA
CCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCEEEEEECCEEECCCCCC
AASGTEPDGFEQFALTARGRVFLSATTEVFARGRYAEGELEIDGFPFPTFTLSDTPEFQE
CCCCCCCCCHHHEEEEECCEEEEEEHHHHHHCCCCCCCEEEECCCCCCEEECCCCCCHHH
TTQYSGAFGLLHDNGPLYLQAAYSFAETERANFDPTFGTAPGFTSDGHSDRIDVKGEYRL
HHCCCCEEEEEECCCCEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCEEE
IGPLLVNFGADAEWTSLETNFTPRQDVRILGGYVQAGIEFGGLSGHIGLRQDDHQRFGGA
EEEEEEECCCCCCCCEEECCCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHCCCC
TSFGADVSYEVAPDVRVKASVGEGFKAPSLFQLFSDFGNELLRPETSTSFDLGLAFRDRS
CCCCCCEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCC
APAYAGITLFRRDSEDLIQFVSCFGVVGGICTGRPFGTYDNVARARAQGVEMEAGFTISD
CCCEECEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEECCCCHHH
ALRGRATYSFIDTEDRSAGALTQGNRLARRPRNAASASLDWDSARGLESLLRPKIGADLR
HHCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEE
YVSGAFDDAFNTVPLESYVVVDLRAELSVAQLNGDHPLVLFARVENVFAEDYQTAAGYAQ
EECCCHHHCCCCCCCCEEEEEEEECCEEEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHH
AGRGVFAGARFSF
CCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11598060 [H]