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The map label for this gene is trmD

Identifier: 85374317

GI number: 85374317

Start: 1545899

End: 1546750

Strand: Reverse

Name: trmD

Synonym: ELI_07450

Alternate gene names: 85374317

Gene position: 1546750-1545899 (Counterclockwise)

Preceding gene: 85374318

Following gene: 85374316

Centisome position: 50.67

GC content: 64.32

Gene sequence:

>852_bases
ATGACCTTCGCCGCCACCATCCTGACGCTTTATCCGGAGATGTTTCCCGGGCCGCTGGGCGTGTCGCTGGCGGGCAAGGC
GCTCGAGCGGGGGGATTGGGTGTGCGAGACCGTCAACCCGCGCGATTTTGCCACCGACAGGCACCGCACGGTCGATGATA
CGCCGGCCGGGGGCGGGGCGGGGATGGTGCTCAAACCGGATGTGATGGCGAGGGCGCTCGATAGCGTCACGTCTCAGGAA
ACCGTTCGGGCTGAGCCTGTCGAAGCCCTGCCTTACACTTCTGGTGCGGCTCCCCAAGAGAAGGACAACCCTTCGACAGG
CTCAGGGCGAACGGATTTTAGGACCGGTCGACCGATACTGGCCATGACACCGCGCGGGAAGCCTATCACCCAAGCCCGCA
TCCGCGAGCTTGCAGCCGGCCCGGGTGTCATCATGCTGTGCGGGCGGTTCGAGGGGTTCGACCAGCGCCTGTTCGACGCC
CGGCCGGAGATCGAAGAGGTGAGCCTCGCCGACATCGTCCTCTCGGGCGGAGAACCTGCCGCGCTGGCCATACTCGACGC
TTGCATTCGGCTGCTTCCGGGGGTAATGGGCGCGCCTTCAAGCGGTACCGAGGAATCCTTCGAAGACGGATTGCTCGAAT
ATCCGCACTACACCCGACCTTACGAATGGGAAGGGCGCACGATCCCTGAAGTGCTGCGATCGGGGGATCATGCGAAGATT
GCCGCCTGGCGCAAGGCCCAGGCGGAAGAGATCACACGGCTACGCAGGCCGGACTTATGGGAGCGTCATCGGGATGTTCG
GGTCCAGTCTGCCTCTGGTGCGCGGCGAAAAGACAAGGAACCGGACCAGTGA

Upstream 100 bases:

>100_bases
CATGGACGAAGGCAGGGAAGGTATTTGTAGGTCCGCTCTTTGTTTTGATCTTCGCCGGGATTTCGTGGCTTTCGAACTTA
GTCGCGGAAAGGGTCATTGA

Downstream 100 bases:

>100_bases
ACCTGATCCAGCAGATCGAAGCGGAAGAAATGGCCGCTGCCGGGAAGGACATTCCCGAATTTCGCGCCGGCGACACCGTT
CGCGTGGGCGTGAAGGTGAC

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTFAATILTLYPEMFPGPLGVSLAGKALERGDWVCETVNPRDFATDRHRTVDDTPAGGGAGMVLKPDVMARALDSVTSQE
TVRAEPVEALPYTSGAAPQEKDNPSTGSGRTDFRTGRPILAMTPRGKPITQARIRELAAGPGVIMLCGRFEGFDQRLFDA
RPEIEEVSLADIVLSGGEPAALAILDACIRLLPGVMGAPSSGTEESFEDGLLEYPHYTRPYEWEGRTIPEVLRSGDHAKI
AAWRKAQAEEITRLRRPDLWERHRDVRVQSASGARRKDKEPDQ

Sequences:

>Translated_283_residues
MTFAATILTLYPEMFPGPLGVSLAGKALERGDWVCETVNPRDFATDRHRTVDDTPAGGGAGMVLKPDVMARALDSVTSQE
TVRAEPVEALPYTSGAAPQEKDNPSTGSGRTDFRTGRPILAMTPRGKPITQARIRELAAGPGVIMLCGRFEGFDQRLFDA
RPEIEEVSLADIVLSGGEPAALAILDACIRLLPGVMGAPSSGTEESFEDGLLEYPHYTRPYEWEGRTIPEVLRSGDHAKI
AAWRKAQAEEITRLRRPDLWERHRDVRVQSASGARRKDKEPDQ
>Mature_282_residues
TFAATILTLYPEMFPGPLGVSLAGKALERGDWVCETVNPRDFATDRHRTVDDTPAGGGAGMVLKPDVMARALDSVTSQET
VRAEPVEALPYTSGAAPQEKDNPSTGSGRTDFRTGRPILAMTPRGKPITQARIRELAAGPGVIMLCGRFEGFDQRLFDAR
PEIEEVSLADIVLSGGEPAALAILDACIRLLPGVMGAPSSGTEESFEDGLLEYPHYTRPYEWEGRTIPEVLRSGDHAKIA
AWRKAQAEEITRLRRPDLWERHRDVRVQSASGARRKDKEPDQ

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=256, Percent_Identity=37.890625, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 30949; Mature: 30818

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFAATILTLYPEMFPGPLGVSLAGKALERGDWVCETVNPRDFATDRHRTVDDTPAGGGA
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCHHCCCCCCCCCCCCCCCCCC
GMVLKPDVMARALDSVTSQETVRAEPVEALPYTSGAAPQEKDNPSTGSGRTDFRTGRPIL
CEEECHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
AMTPRGKPITQARIRELAAGPGVIMLCGRFEGFDQRLFDARPEIEEVSLADIVLSGGEPA
EECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHEEECCCCCH
ALAILDACIRLLPGVMGAPSSGTEESFEDGLLEYPHYTRPYEWEGRTIPEVLRSGDHAKI
HHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHH
AAWRKAQAEEITRLRRPDLWERHRDVRVQSASGARRKDKEPDQ
HHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TFAATILTLYPEMFPGPLGVSLAGKALERGDWVCETVNPRDFATDRHRTVDDTPAGGGA
CHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECCCCHHCCCCCCCCCCCCCCCCCC
GMVLKPDVMARALDSVTSQETVRAEPVEALPYTSGAAPQEKDNPSTGSGRTDFRTGRPIL
CEEECHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
AMTPRGKPITQARIRELAAGPGVIMLCGRFEGFDQRLFDARPEIEEVSLADIVLSGGEPA
EECCCCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHEEECCCCCH
ALAILDACIRLLPGVMGAPSSGTEESFEDGLLEYPHYTRPYEWEGRTIPEVLRSGDHAKI
HHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHH
AAWRKAQAEEITRLRRPDLWERHRDVRVQSASGARRKDKEPDQ
HHHHHHHHHHHHHHCCCCHHHHHHCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA