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The map label for this gene is dnaN [H]

Identifier: 85374293

GI number: 85374293

Start: 1523467

End: 1524594

Strand: Reverse

Name: dnaN [H]

Synonym: ELI_07330

Alternate gene names: 85374293

Gene position: 1524594-1523467 (Counterclockwise)

Preceding gene: 85374294

Following gene: 85374292

Centisome position: 49.95

GC content: 62.41

Gene sequence:

>1128_bases
ATGAAGGCCACCATCGAACGCGCAACGCTCCTCCGTTGCCTCTCCCACGTCCAGTCGGTTGTCGAACGCCGCAACACGAT
CCCGATCCTGTCGAACATCCTGATCGAGGCCGGAGACGGCAGCGCGGTCAAGGTCATGGCGACCGACCTCGATCTGCAGG
TCGTCGAAACGATGAACGCCGCAAGCGTCGAATCCTCCGGCTCGACCACCGTTTCCGCCCACCTATTGTTCGACATCGCC
CGCAAATTGCCCGACGGCAGCCAGGTGAGCCTCGAAGTCGCCGACAACCGGATGACGATCAAGGCCGGGCGCAGCCGCTT
CCAGCTGCCGACCCTGCCGAAGGACGATTTCCCCGTCATCGTGGAAGGCGATTTGCCGACCAGCTTCGAAATCCCGGCCA
AGACCCTCGCCGAACTGATCGACCGCACGCGCTTCGCGATCAGCACCGAGGAAACGCGCTATTACCTCAACGGCATCTTC
CTGCATGTGTCGGACGAGGACACGCCGGTTCTTAAAGCCGCAGCCACCGATGGTCATCGCCTTGCGCGCTTCACGCTCGC
CCGCCCCGACGGCGCGGACGGCATGCCGGATGTCATCGTCCCGCGCAAAGCCGTGGGCGAGCTGCGCAAGCTGCTGGAAG
AGCGGATGGACGGCAATGTCGTCGTCGACCTTTCGGCCAGCAAGATCCGTTTCACGCTCGGTGGCGAGGGCGGCACGGTA
CTCACCTCCAAGCTGATCGACGGCACCTTCCCCGATTACAGCCGTGTGATCCCGACCGGCAACGACAAGCTGCTCAAAGT
CGACCCGAAGAGTTTCTTCGAAGGCGTTGATCGCGTCGCCACCATCGCCACGGAAAAGACCCGCGCGGTGAAGATGGGTC
TGGATGCCGACAAGGTAACGCTTTCGGTCACATCGCCCGATAACGGCACCGCGTCGGAGGAACTCGCGGCCGAATACAAG
TCCGAAGGGTTCGAGATCGGCTTCAACGCCAATTACCTCAAGGACATCCTCAGCCAGATCGACAGCGACATGGTCGAATT
GCACCTGGCCGACGCGGGCGCCCCAACGCTGATCCGCAAGGACGAGAACAGCCCGGCACTTTATGTGCTGATGCCGATGC
GGGTGTAG

Upstream 100 bases:

>100_bases
GCAGGTGCGCTAGGGTTTATGGGCAATTTACCCGGCGCCCGGGGCGATTCCGGTGGGGTTTTTGCACCTTGTGGGCGCCC
ATAAAACGAGAGAAAGACCG

Downstream 100 bases:

>100_bases
CCGCCGCAATATTCTAGGCTCGCCTCGACTATCCCAAGTGGCTAGGGTCGCCTCAAGCAAAGAGGAGACCCCCATGGCTC
AAGCCGTCCACGTCCAGAAA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKATIERATLLRCLSHVQSVVERRNTIPILSNILIEAGDGSAVKVMATDLDLQVVETMNAASVESSGSTTVSAHLLFDIA
RKLPDGSQVSLEVADNRMTIKAGRSRFQLPTLPKDDFPVIVEGDLPTSFEIPAKTLAELIDRTRFAISTEETRYYLNGIF
LHVSDEDTPVLKAAATDGHRLARFTLARPDGADGMPDVIVPRKAVGELRKLLEERMDGNVVVDLSASKIRFTLGGEGGTV
LTSKLIDGTFPDYSRVIPTGNDKLLKVDPKSFFEGVDRVATIATEKTRAVKMGLDADKVTLSVTSPDNGTASEELAAEYK
SEGFEIGFNANYLKDILSQIDSDMVELHLADAGAPTLIRKDENSPALYVLMPMRV

Sequences:

>Translated_375_residues
MKATIERATLLRCLSHVQSVVERRNTIPILSNILIEAGDGSAVKVMATDLDLQVVETMNAASVESSGSTTVSAHLLFDIA
RKLPDGSQVSLEVADNRMTIKAGRSRFQLPTLPKDDFPVIVEGDLPTSFEIPAKTLAELIDRTRFAISTEETRYYLNGIF
LHVSDEDTPVLKAAATDGHRLARFTLARPDGADGMPDVIVPRKAVGELRKLLEERMDGNVVVDLSASKIRFTLGGEGGTV
LTSKLIDGTFPDYSRVIPTGNDKLLKVDPKSFFEGVDRVATIATEKTRAVKMGLDADKVTLSVTSPDNGTASEELAAEYK
SEGFEIGFNANYLKDILSQIDSDMVELHLADAGAPTLIRKDENSPALYVLMPMRV
>Mature_375_residues
MKATIERATLLRCLSHVQSVVERRNTIPILSNILIEAGDGSAVKVMATDLDLQVVETMNAASVESSGSTTVSAHLLFDIA
RKLPDGSQVSLEVADNRMTIKAGRSRFQLPTLPKDDFPVIVEGDLPTSFEIPAKTLAELIDRTRFAISTEETRYYLNGIF
LHVSDEDTPVLKAAATDGHRLARFTLARPDGADGMPDVIVPRKAVGELRKLLEERMDGNVVVDLSASKIRFTLGGEGGTV
LTSKLIDGTFPDYSRVIPTGNDKLLKVDPKSFFEGVDRVATIATEKTRAVKMGLDADKVTLSVTSPDNGTASEELAAEYK
SEGFEIGFNANYLKDILSQIDSDMVELHLADAGAPTLIRKDENSPALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=377, Percent_Identity=35.8090185676393, Blast_Score=238, Evalue=5e-64,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40751; Mature: 40751

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATIERATLLRCLSHVQSVVERRNTIPILSNILIEAGDGSAVKVMATDLDLQVVETMNA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCEEEEHHCCC
ASVESSGSTTVSAHLLFDIARKLPDGSQVSLEVADNRMTIKAGRSRFQLPTLPKDDFPVI
CCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCEE
VEGDLPTSFEIPAKTLAELIDRTRFAISTEETRYYLNGIFLHVSDEDTPVLKAAATDGHR
EECCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCEEEEECCCCCE
LARFTLARPDGADGMPDVIVPRKAVGELRKLLEERMDGNVVVDLSASKIRFTLGGEGGTV
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEE
LTSKLIDGTFPDYSRVIPTGNDKLLKVDPKSFFEGVDRVATIATEKTRAVKMGLDADKVT
EEHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
LSVTSPDNGTASEELAAEYKSEGFEIGFNANYLKDILSQIDSDMVELHLADAGAPTLIRK
EEEECCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEE
DENSPALYVLMPMRV
CCCCCEEEEEEECCC
>Mature Secondary Structure
MKATIERATLLRCLSHVQSVVERRNTIPILSNILIEAGDGSAVKVMATDLDLQVVETMNA
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCEEEEHHCCC
ASVESSGSTTVSAHLLFDIARKLPDGSQVSLEVADNRMTIKAGRSRFQLPTLPKDDFPVI
CCCCCCCCCEEHHHHHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCCCCCCCCCCCCEE
VEGDLPTSFEIPAKTLAELIDRTRFAISTEETRYYLNGIFLHVSDEDTPVLKAAATDGHR
EECCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEEEEEECCCCCCEEEEECCCCCE
LARFTLARPDGADGMPDVIVPRKAVGELRKLLEERMDGNVVVDLSASKIRFTLGGEGGTV
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCEE
LTSKLIDGTFPDYSRVIPTGNDKLLKVDPKSFFEGVDRVATIATEKTRAVKMGLDADKVT
EEHHHHCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEE
LSVTSPDNGTASEELAAEYKSEGFEIGFNANYLKDILSQIDSDMVELHLADAGAPTLIRK
EEEECCCCCCCHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCEEEEE
DENSPALYVLMPMRV
CCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]