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The map label for this gene is proB [H]

Identifier: 85374274

GI number: 85374274

Start: 1496088

End: 1497323

Strand: Reverse

Name: proB [H]

Synonym: ELI_07235

Alternate gene names: 85374274

Gene position: 1497323-1496088 (Counterclockwise)

Preceding gene: 85374277

Following gene: 85374273

Centisome position: 49.05

GC content: 64.97

Gene sequence:

>1236_bases
TTGACCCAGCAGACCCGCACAGCTTCGCCCGACACCACCAAATCGCGAAAGATCCCGGAACCGCGCAAGATCGTCGTCAA
GGTCGGCTCCGCGCTGCTCGCCAATACCGAGCGGCTGACTCCGCGCTTCGGCTTCATACAGCGCCTGATGGAGGATATCG
CCCGGCTGCGTGCGGAGGGGCACGAAGTGATCCTGTGCTCGAGCGGGGCCGTGGCGCTGGGCATGAAGGCGGTCGGCGTC
GAGCCGGGCGAGGCCGGATTGACCGACAAGCAGGCGGCCGCGGCCTGCGGCATGCCCCAGCTGCTCAACGCCTACAAGCA
GGTCGGATACGAATTCGATTTCGAGATCGCGCAGATCCTGCTGACGCTGGGCGACTTCGAGGATCACCGCCGCTTCCTCA
ACACCAAGAACACCGTCCACCGCCTGCTGCAGTCGAACATCATCCCGATCGTCAACGAGAACGATACAATTACGACCGAA
GAAATCCGCGTCGGCGATAACGACCGGCTGGCGGCCAAGATCGCGCAGATGGTGCAGGCCGACGATTTCGTGATCCTGAC
CACCATCGACGGGCTGTACGATCGCAATCCGGACGATCCGGACGCAAAGCTGGTCGAGGAAGTCAGCGACGTGAGCGAAT
ACCTGGCCGCGACGGCCGGCAAGACCACGCTCGGAACGGGCGGCATGCTGACCAAGCTGCAGGCGGCGAACATGGCGCAG
AACGCGGGCTGCACCGCGTGGATTGCCGATGGTGAGGAACCGCGGCCGATTTCAGCCATCCTGAACGGCGAGCGGCGCTG
CACCAAGTGCCTGCCCAATCCCAATCCGGCCTCGGTCTGGGATACCTGGCTGGCCGACCGCCTGCAAATGGCCGGGCATC
TCGTCATCGGCGACGAGGCAGCGGCGAAGCTTGGCAGCGGCGCACCGATCCACCGCGACGACGTGCTGTCGATGGATGGC
GATTTCAGCCGCGGCGACGTGCTCCACATCTACGACAAGGACGGCGTGGAGCGTGCCCGCGGCCTGACTGACTTCACCAG
CGAGGAGACGCGGGTGATGATCAACAACCCGGACCTGCCGGCCGAACAACTGCTCGGCTACCAGACCCACGCCGAGATTA
TCCGCGGCAACAACCTCGTCGCGCTCGAAGGGCATCACCTGACCTGGGATGCGCCCGAAGGCGAAAGCGGCCAGCGCCGC
GTCGCACCGCTCGAAGACGATCCGATGGCGGACTGA

Upstream 100 bases:

>100_bases
AAGTTGCTGCATTGCAACATAGGTAGTCCTGCGAGAATTGTAACCGTTAGCGGCGCAGGCAGACACGACTGACCGCCCGC
CACCGAGAATAAGGCACGTT

Downstream 100 bases:

>100_bases
TCCCCGCCTGCGCGCCGGTTCCGATCATTCCCCAGTTTTGGCTGCCGACGGCTCACAACTTCACTTGGCGTTCAGCGGGG
GCAGGGCTATAGCGCCACCC

Product: hypothetical protein

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MTQQTRTASPDTTKSRKIPEPRKIVVKVGSALLANTERLTPRFGFIQRLMEDIARLRAEGHEVILCSSGAVALGMKAVGV
EPGEAGLTDKQAAAACGMPQLLNAYKQVGYEFDFEIAQILLTLGDFEDHRRFLNTKNTVHRLLQSNIIPIVNENDTITTE
EIRVGDNDRLAAKIAQMVQADDFVILTTIDGLYDRNPDDPDAKLVEEVSDVSEYLAATAGKTTLGTGGMLTKLQAANMAQ
NAGCTAWIADGEEPRPISAILNGERRCTKCLPNPNPASVWDTWLADRLQMAGHLVIGDEAAAKLGSGAPIHRDDVLSMDG
DFSRGDVLHIYDKDGVERARGLTDFTSEETRVMINNPDLPAEQLLGYQTHAEIIRGNNLVALEGHHLTWDAPEGESGQRR
VAPLEDDPMAD

Sequences:

>Translated_411_residues
MTQQTRTASPDTTKSRKIPEPRKIVVKVGSALLANTERLTPRFGFIQRLMEDIARLRAEGHEVILCSSGAVALGMKAVGV
EPGEAGLTDKQAAAACGMPQLLNAYKQVGYEFDFEIAQILLTLGDFEDHRRFLNTKNTVHRLLQSNIIPIVNENDTITTE
EIRVGDNDRLAAKIAQMVQADDFVILTTIDGLYDRNPDDPDAKLVEEVSDVSEYLAATAGKTTLGTGGMLTKLQAANMAQ
NAGCTAWIADGEEPRPISAILNGERRCTKCLPNPNPASVWDTWLADRLQMAGHLVIGDEAAAKLGSGAPIHRDDVLSMDG
DFSRGDVLHIYDKDGVERARGLTDFTSEETRVMINNPDLPAEQLLGYQTHAEIIRGNNLVALEGHHLTWDAPEGESGQRR
VAPLEDDPMAD
>Mature_410_residues
TQQTRTASPDTTKSRKIPEPRKIVVKVGSALLANTERLTPRFGFIQRLMEDIARLRAEGHEVILCSSGAVALGMKAVGVE
PGEAGLTDKQAAAACGMPQLLNAYKQVGYEFDFEIAQILLTLGDFEDHRRFLNTKNTVHRLLQSNIIPIVNENDTITTEE
IRVGDNDRLAAKIAQMVQADDFVILTTIDGLYDRNPDDPDAKLVEEVSDVSEYLAATAGKTTLGTGGMLTKLQAANMAQN
AGCTAWIADGEEPRPISAILNGERRCTKCLPNPNPASVWDTWLADRLQMAGHLVIGDEAAAKLGSGAPIHRDDVLSMDGD
FSRGDVLHIYDKDGVERARGLTDFTSEETRVMINNPDLPAEQLLGYQTHAEIIRGNNLVALEGHHLTWDAPEGESGQRRV
APLEDDPMAD

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI21361368, Length=295, Percent_Identity=29.1525423728814, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI62912457, Length=293, Percent_Identity=29.3515358361775, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1786437, Length=355, Percent_Identity=34.3661971830986, Blast_Score=198, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17569969, Length=279, Percent_Identity=30.4659498207885, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17569967, Length=277, Percent_Identity=30.6859205776173, Blast_Score=140, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320506, Length=243, Percent_Identity=39.5061728395062, Blast_Score=166, Evalue=4e-42,
Organism=Saccharomyces cerevisiae, GI6321822, Length=244, Percent_Identity=36.4754098360656, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=29.4117647058824, Blast_Score=128, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 44817; Mature: 44686

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTRTASPDTTKSRKIPEPRKIVVKVGSALLANTERLTPRFGFIQRLMEDIARLRAEG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCC
HEVILCSSGAVALGMKAVGVEPGEAGLTDKQAAAACGMPQLLNAYKQVGYEFDFEIAQIL
CEEEEECCCCCEECEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHH
LTLGDFEDHRRFLNTKNTVHRLLQSNIIPIVNENDTITTEEIRVGDNDRLAAKIAQMVQA
HHHCCCHHHHHHHCCHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHCC
DDFVILTTIDGLYDRNPDDPDAKLVEEVSDVSEYLAATAGKTTLGTGGMLTKLQAANMAQ
CCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
NAGCTAWIADGEEPRPISAILNGERRCTKCLPNPNPASVWDTWLADRLQMAGHLVIGDEA
CCCCEEEECCCCCCCCHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECCHH
AAKLGSGAPIHRDDVLSMDGDFSRGDVLHIYDKDGVERARGLTDFTSEETRVMINNPDLP
HHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCEEEECCCCCC
AEQLLGYQTHAEIIRGNNLVALEGHHLTWDAPEGESGQRRVAPLEDDPMAD
HHHHHCHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQQTRTASPDTTKSRKIPEPRKIVVKVGSALLANTERLTPRFGFIQRLMEDIARLRAEG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHCCC
HEVILCSSGAVALGMKAVGVEPGEAGLTDKQAAAACGMPQLLNAYKQVGYEFDFEIAQIL
CEEEEECCCCCEECEEEECCCCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHH
LTLGDFEDHRRFLNTKNTVHRLLQSNIIPIVNENDTITTEEIRVGDNDRLAAKIAQMVQA
HHHCCCHHHHHHHCCHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCHHHHHHHHHHCC
DDFVILTTIDGLYDRNPDDPDAKLVEEVSDVSEYLAATAGKTTLGTGGMLTKLQAANMAQ
CCEEEEEEECHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
NAGCTAWIADGEEPRPISAILNGERRCTKCLPNPNPASVWDTWLADRLQMAGHLVIGDEA
CCCCEEEECCCCCCCCHHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEECCHH
AAKLGSGAPIHRDDVLSMDGDFSRGDVLHIYDKDGVERARGLTDFTSEETRVMINNPDLP
HHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHCCCCCCCCCCCEEEECCCCCC
AEQLLGYQTHAEIIRGNNLVALEGHHLTWDAPEGESGQRRVAPLEDDPMAD
HHHHHCHHHHHHHHCCCCEEEEECCEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA