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The map label for this gene is ilvD [H]

Identifier: 85374209

GI number: 85374209

Start: 1432588

End: 1434309

Strand: Reverse

Name: ilvD [H]

Synonym: ELI_06910

Alternate gene names: 85374209

Gene position: 1434309-1432588 (Counterclockwise)

Preceding gene: 85374210

Following gene: 85374208

Centisome position: 46.99

GC content: 63.41

Gene sequence:

>1722_bases
ATGTCGTCGAGATTCGACAAGTCCAAGCTGCCCAGCCGCCACGTTTCGGTCGGGCCAGAGCGCGCGCCGCATCGTTCGTA
TTACTACGCGATGGGTCTGACCGAGCAGGAGATCGCGCGACCCTTCGTCGGCATTGTTAGCGCGGGCAACGACAGCGCGC
CCTGCAACACGACGCTCGATGCGCAGGCGGACATCTGTCGCACTGGCGTCAACCAAGCAGGCGGCATGCCGCGGCGCTTC
AACACCATCACCGTGACGGACGGTATCGCCATGGGACACCAGGGCATGAAAAGCTCGCTGGTCAGCCGCGAGGTGATTGC
GGACTCGATCGAGCTATCGGTGCGCGGGCATTGCTATGATGCGCTGGTCGGATTCGCCGGCTGCGACAAGAGCTTGCCCG
GCATGATGATGGCCATGCTGCGGCTCAATGTTCCGAGCATCTTCGTCTATGGCGGATCGATCCTGCCGGGCCGCTTCCAC
GACAAGGACGTGACCGTCGTCGACGTGTTCGAAGCGGTCGGCCAGCACGCGGCGGGCAATTGTCCGCTGAAAGAACTGAC
CGCGCTGGAAAAGGTCGCCTGTCCCGGGCACGGCGCGTGCGGCGGGCAGTTTACCGCCAACACCATGGCCTGCGTCGGCG
AAGCGATCGGCCTGTCGCTGCCCAACAGCAATATGGCCCCCGCGCCCTATCGTTCGCGCGAGGAGATCGCGGTGGCGGCG
GGGCGGCAGGTCATGACGCTGCTGGAGAAAAACCTGCGCCCGCGTGACATCTGCACAAGGGCCGCATTCGAGAATGCAGC
ACGGGTCGTCGCGGCAACGGGCGGCTCGACCAATGCCGCTTTGCACCTGCCTGCCATGGCCCATGAATGCGGCATCGACT
TCGACCTGTTCGATGTCGCCGAGACATTCAAATCAACACCTTATATCGCAGACCTCAAACCTGGCGGGCGCTATGTCGCC
AAGGACATGCACGAGGCGGGCGGGGTCTACATGGCGATGAAAACGCTGCTGGAGGGCGGTTTCCTCGATCCCGAACCGAT
GACCGTCACAGGCAAGACGCTGGGCGAGAATATCGAGGAGATCACCTGGGACCCCGACCAGAAGGTTTTCTACGACGTAT
CCAATCCGATCACGCCGACCGGCGGCGTCGTCGGGCTCAGGGGTTCTCTCGCGCCCGACGGGGCGATTGTGAAGGTGGCG
GGAATGGAACGGCTCCAGTTCAGCGGCCCAGCGCGAGTATTCGATTGCGAGGAGGATGCCTTCAAGGCGGTCGAGCAGCG
CGACATCGCCGAAGGTTGCGTCATCGTAATCCGCTACGAAGGCCCCAAGGGCGGCCCCGGCATGCGCGAAATGCTCTCTA
CTACCGCAGCGCTCTACGGGCAGGGCATGGGCGAGAAAGTGGCGCTCATCACCGACGGGCGCTTCTCCGGCGCGACGCGC
GGCTTTTGCATCGGCCATGTCGGCCCGGAAGCGGCAGACTGCGGCCCGATTGCGCTGGTCGAGGATGGCGATACAATCAG
CATCGATGCCGTTGCAGGCACGATTGAGCTCGACGTCGATGCCGAAACGCTTGAAAAACGGCGTCAAAGCTGGACACCGC
GGGCGAACGATTACCAGTCCGGCGCGCTGTGGCGCTATGCGAGGAACGTCGGTCCTGCGCATGAGGGCGCAGTGACTCAT
CCGGGAGCGAAATCGGAACGCCATGTCTTTGCGGATATCTAG

Upstream 100 bases:

>100_bases
ATTAGCCCGTCATCCCAGCGAAAGCCGGGATCGCTGTCCGCCAAGTCGGACATGTCCGCATGAGACCCCAGCCTTCGCTG
GGGTGACGGGAGGGATGTGA

Downstream 100 bases:

>100_bases
CGCGCTGGTGTGCACGGCTGCTCTTGTCGCCTGCTCCGGCGACGCGGTGGACGAGACGGCCGTCGAACCGGGCGACCAGA
TGCTCGCCTGCGCCATCGGC

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSSRFDKSKLPSRHVSVGPERAPHRSYYYAMGLTEQEIARPFVGIVSAGNDSAPCNTTLDAQADICRTGVNQAGGMPRRF
NTITVTDGIAMGHQGMKSSLVSREVIADSIELSVRGHCYDALVGFAGCDKSLPGMMMAMLRLNVPSIFVYGGSILPGRFH
DKDVTVVDVFEAVGQHAAGNCPLKELTALEKVACPGHGACGGQFTANTMACVGEAIGLSLPNSNMAPAPYRSREEIAVAA
GRQVMTLLEKNLRPRDICTRAAFENAARVVAATGGSTNAALHLPAMAHECGIDFDLFDVAETFKSTPYIADLKPGGRYVA
KDMHEAGGVYMAMKTLLEGGFLDPEPMTVTGKTLGENIEEITWDPDQKVFYDVSNPITPTGGVVGLRGSLAPDGAIVKVA
GMERLQFSGPARVFDCEEDAFKAVEQRDIAEGCVIVIRYEGPKGGPGMREMLSTTAALYGQGMGEKVALITDGRFSGATR
GFCIGHVGPEAADCGPIALVEDGDTISIDAVAGTIELDVDAETLEKRRQSWTPRANDYQSGALWRYARNVGPAHEGAVTH
PGAKSERHVFADI

Sequences:

>Translated_573_residues
MSSRFDKSKLPSRHVSVGPERAPHRSYYYAMGLTEQEIARPFVGIVSAGNDSAPCNTTLDAQADICRTGVNQAGGMPRRF
NTITVTDGIAMGHQGMKSSLVSREVIADSIELSVRGHCYDALVGFAGCDKSLPGMMMAMLRLNVPSIFVYGGSILPGRFH
DKDVTVVDVFEAVGQHAAGNCPLKELTALEKVACPGHGACGGQFTANTMACVGEAIGLSLPNSNMAPAPYRSREEIAVAA
GRQVMTLLEKNLRPRDICTRAAFENAARVVAATGGSTNAALHLPAMAHECGIDFDLFDVAETFKSTPYIADLKPGGRYVA
KDMHEAGGVYMAMKTLLEGGFLDPEPMTVTGKTLGENIEEITWDPDQKVFYDVSNPITPTGGVVGLRGSLAPDGAIVKVA
GMERLQFSGPARVFDCEEDAFKAVEQRDIAEGCVIVIRYEGPKGGPGMREMLSTTAALYGQGMGEKVALITDGRFSGATR
GFCIGHVGPEAADCGPIALVEDGDTISIDAVAGTIELDVDAETLEKRRQSWTPRANDYQSGALWRYARNVGPAHEGAVTH
PGAKSERHVFADI
>Mature_572_residues
SSRFDKSKLPSRHVSVGPERAPHRSYYYAMGLTEQEIARPFVGIVSAGNDSAPCNTTLDAQADICRTGVNQAGGMPRRFN
TITVTDGIAMGHQGMKSSLVSREVIADSIELSVRGHCYDALVGFAGCDKSLPGMMMAMLRLNVPSIFVYGGSILPGRFHD
KDVTVVDVFEAVGQHAAGNCPLKELTALEKVACPGHGACGGQFTANTMACVGEAIGLSLPNSNMAPAPYRSREEIAVAAG
RQVMTLLEKNLRPRDICTRAAFENAARVVAATGGSTNAALHLPAMAHECGIDFDLFDVAETFKSTPYIADLKPGGRYVAK
DMHEAGGVYMAMKTLLEGGFLDPEPMTVTGKTLGENIEEITWDPDQKVFYDVSNPITPTGGVVGLRGSLAPDGAIVKVAG
MERLQFSGPARVFDCEEDAFKAVEQRDIAEGCVIVIRYEGPKGGPGMREMLSTTAALYGQGMGEKVALITDGRFSGATRG
FCIGHVGPEAADCGPIALVEDGDTISIDAVAGTIELDVDAETLEKRRQSWTPRANDYQSGALWRYARNVGPAHEGAVTHP
GAKSERHVFADI

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=37.7887788778878, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1786464, Length=476, Percent_Identity=33.6134453781513, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI2367371, Length=531, Percent_Identity=31.4500941619586, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788157, Length=519, Percent_Identity=29.8651252408478, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=544, Percent_Identity=43.9338235294118, Blast_Score=427, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 60953; Mature: 60822

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRFDKSKLPSRHVSVGPERAPHRSYYYAMGLTEQEIARPFVGIVSAGNDSAPCNTTLD
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCEEEEECCCCCCCCCCCCC
AQADICRTGVNQAGGMPRRFNTITVTDGIAMGHQGMKSSLVSREVIADSIELSVRGHCYD
CCHHHHHHCCHHHCCCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHCEEEEECCCHHH
ALVGFAGCDKSLPGMMMAMLRLNVPSIFVYGGSILPGRFHDKDVTVVDVFEAVGQHAAGN
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCC
CPLKELTALEKVACPGHGACGGQFTANTMACVGEAIGLSLPNSNMAPAPYRSREEIAVAA
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHC
GRQVMTLLEKNLRPRDICTRAAFENAARVVAATGGSTNAALHLPAMAHECGIDFDLFDVA
CHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHHCCCCCHHHHHH
ETFKSTPYIADLKPGGRYVAKDMHEAGGVYMAMKTLLEGGFLDPEPMTVTGKTLGENIEE
HHHCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECHHHHCCHHH
ITWDPDQKVFYDVSNPITPTGGVVGLRGSLAPDGAIVKVAGMERLQFSGPARVFDCEEDA
CCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEECCCCCEEEECCHHH
FKAVEQRDIAEGCVIVIRYEGPKGGPGMREMLSTTAALYGQGMGEKVALITDGRFSGATR
HHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GFCIGHVGPEAADCGPIALVEDGDTISIDAVAGTIELDVDAETLEKRRQSWTPRANDYQS
CEEEECCCCCCCCCCCEEEEECCCEEEEEEEEEEEEEECCHHHHHHHHHCCCCCCCCCCC
GALWRYARNVGPAHEGAVTHPGAKSERHVFADI
CHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
SSRFDKSKLPSRHVSVGPERAPHRSYYYAMGLTEQEIARPFVGIVSAGNDSAPCNTTLD
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHCCCEEEEECCCCCCCCCCCCC
AQADICRTGVNQAGGMPRRFNTITVTDGIAMGHQGMKSSLVSREVIADSIELSVRGHCYD
CCHHHHHHCCHHHCCCCCCCEEEEEECCHHHCCCHHHHHHHHHHHHHHCEEEEECCCHHH
ALVGFAGCDKSLPGMMMAMLRLNVPSIFVYGGSILPGRFHDKDVTVVDVFEAVGQHAAGN
HHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCC
CPLKELTALEKVACPGHGACGGQFTANTMACVGEAIGLSLPNSNMAPAPYRSREEIAVAA
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHC
GRQVMTLLEKNLRPRDICTRAAFENAARVVAATGGSTNAALHLPAMAHECGIDFDLFDVA
CHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEECCHHHHHCCCCCHHHHHH
ETFKSTPYIADLKPGGRYVAKDMHEAGGVYMAMKTLLEGGFLDPEPMTVTGKTLGENIEE
HHHCCCCEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEEECHHHHCCHHH
ITWDPDQKVFYDVSNPITPTGGVVGLRGSLAPDGAIVKVAGMERLQFSGPARVFDCEEDA
CCCCCCCCEEEECCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEECCCCCEEEECCHHH
FKAVEQRDIAEGCVIVIRYEGPKGGPGMREMLSTTAALYGQGMGEKVALITDGRFSGATR
HHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCC
GFCIGHVGPEAADCGPIALVEDGDTISIDAVAGTIELDVDAETLEKRRQSWTPRANDYQS
CEEEECCCCCCCCCCCEEEEECCCEEEEEEEEEEEEEECCHHHHHHHHHCCCCCCCCCCC
GALWRYARNVGPAHEGAVTHPGAKSERHVFADI
CHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA