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The map label for this gene is lcfB [H]

Identifier: 85374201

GI number: 85374201

Start: 1425279

End: 1426805

Strand: Reverse

Name: lcfB [H]

Synonym: ELI_06870

Alternate gene names: 85374201

Gene position: 1426805-1425279 (Counterclockwise)

Preceding gene: 85374206

Following gene: 85374200

Centisome position: 46.74

GC content: 63.06

Gene sequence:

>1527_bases
ATGTCCCAAACGCCTGACCCGACGCCAAAGCCGCTCGATCACCTTGCCGAATGCGGCGAGGGCGATGCTCCGGCGCTGGT
CTTGCGCGGTGAGACACTTACCTATGAGGACTTAAGAAGCCGTGTCGCTGCACTGGCTTCGTGGCTGCAGGCTCAGGTCC
CCGAAGAGGGCGAGCGGATTGCGACGTGGGCCGCAAAGGGCGCGCTGACGTGCCTGATGCCGCTCGCCTGTGCGCGGGCC
GGACTGGTGCATGTGCCGATCAATCCGCTGCTCAAGCGGGCACAGGTCGCGCATATTTTGGCCGATAGCGGCGCGCTGTT
GCTGCTGGGGACGGCGGCGCGATTGAAGTCGCTGGAACAGGGCGATGTGCCCGCCGGATGCACCCCGATGGACGAAAAGG
CGGTATGGGAGGCAGTCGAGGCGCATGAAGGCGCTTTGAAACCTTCAGACGCCGATCCGGATGCGCTGGCGGCGATCCTT
TACACCAGCGGTTCCACCGGGCGGCCCAAGGGCGTCATGCTGAGCCATGCGAATATGTGGTTGGGGGCGGTTAGCGTGGC
CCATTATCTCGGCATGGAGAGCGATGACGTGACGCTCGGCGTGCTGCCGCTCAGCTTCGATTACGGGCAGAACCAGCTGC
TTTCGACCTGGTATACCGGCGGCAGCGTGGTGCCGCTCGATTACTTATTCCCGAACGATGTCGTAAAGGCCTGTGCGAAA
CACAACATCACGACGCTCGCGGCGGTACCGCCTCTGTGGGTCCAGCTGACCGAGATCGAATGGCCGTCTGAAGCAGTCGC
ATCGATGCGACGGATGACCAATTCGGGTGGCGCGCTGACCGAAGATCTCGTGCGAGATTTGCGCGCTATCTTCCCGCAGA
CACGGCTTTTTCCGATGTACGGCCTGACCGAAGCCTTCCGTTCGACATATCTCGATCCTTCATTGGTGGACACGCATCCG
ACTTCGATGGGGACGGCGATTCCCTTTGCAGAGATTCTCGTGATCGACGACAATGGCGAAGTGGCGCAGGCCGGTGAGGA
GGGCGAACTCGTCCATTGCGGCCCGCTGGTGGCGCAGGGATATTGGCAGGATGCCGAGCGCACTGCCGAGCGATTCAAGT
CCGCACCGGATGGCTCACATTATGGCGGGACTGCCGTCTGGTCCGGAGATCGCGTGAAGCGAAACGCCGACGGCCTGCTT
TACTTCGTCGGTCGCCGCGACGCGATGATCAAGAGTGCGGGCAATCGCATCAGTCCGCAGGAGATCGAGGACGCAGCGAT
CGCCACCGGACTGGTCGCCGAGGCGGTGGCGCTGGGCGTCAAGGATGCCAGGCTGGGCCATGCCGTCCATCTGGTCGTGC
GCGCAGCGCCCGAAGCGGACGACCCGCAGGGCAAGCTTTTGCCGCGGCTTGTCAAGGAACTGCCCAATTTCATGTTGCCG
CAGAAGGTCCATTGGCGCGAGACCCTGCCGCTCAATCCAAACGGCAAGCTTGACCGTACCGCGATCGCGCGCGAGATCGG
GGCATGA

Upstream 100 bases:

>100_bases
CGCGTCTTTTGGCATCATGCCTTGCAAGACGTTAACCCTGTCGTGATAGCTGGCAGCGATTGCAGTCACAAAAACCCCAC
AAAACCCTCTCGGAAACCCG

Downstream 100 bases:

>100_bases
GCAGCGCTGCCGCAGAGACGGAAAAGCCGCCTGGCAAGCCGCTGGGGCCGATACCGGAGAATTTCGAGGCACAGGACGGC
GAGTTGGCAATCGGCGGCAA

Product: AMP-binding domain-containing protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSQTPDPTPKPLDHLAECGEGDAPALVLRGETLTYEDLRSRVAALASWLQAQVPEEGERIATWAAKGALTCLMPLACARA
GLVHVPINPLLKRAQVAHILADSGALLLLGTAARLKSLEQGDVPAGCTPMDEKAVWEAVEAHEGALKPSDADPDALAAIL
YTSGSTGRPKGVMLSHANMWLGAVSVAHYLGMESDDVTLGVLPLSFDYGQNQLLSTWYTGGSVVPLDYLFPNDVVKACAK
HNITTLAAVPPLWVQLTEIEWPSEAVASMRRMTNSGGALTEDLVRDLRAIFPQTRLFPMYGLTEAFRSTYLDPSLVDTHP
TSMGTAIPFAEILVIDDNGEVAQAGEEGELVHCGPLVAQGYWQDAERTAERFKSAPDGSHYGGTAVWSGDRVKRNADGLL
YFVGRRDAMIKSAGNRISPQEIEDAAIATGLVAEAVALGVKDARLGHAVHLVVRAAPEADDPQGKLLPRLVKELPNFMLP
QKVHWRETLPLNPNGKLDRTAIAREIGA

Sequences:

>Translated_508_residues
MSQTPDPTPKPLDHLAECGEGDAPALVLRGETLTYEDLRSRVAALASWLQAQVPEEGERIATWAAKGALTCLMPLACARA
GLVHVPINPLLKRAQVAHILADSGALLLLGTAARLKSLEQGDVPAGCTPMDEKAVWEAVEAHEGALKPSDADPDALAAIL
YTSGSTGRPKGVMLSHANMWLGAVSVAHYLGMESDDVTLGVLPLSFDYGQNQLLSTWYTGGSVVPLDYLFPNDVVKACAK
HNITTLAAVPPLWVQLTEIEWPSEAVASMRRMTNSGGALTEDLVRDLRAIFPQTRLFPMYGLTEAFRSTYLDPSLVDTHP
TSMGTAIPFAEILVIDDNGEVAQAGEEGELVHCGPLVAQGYWQDAERTAERFKSAPDGSHYGGTAVWSGDRVKRNADGLL
YFVGRRDAMIKSAGNRISPQEIEDAAIATGLVAEAVALGVKDARLGHAVHLVVRAAPEADDPQGKLLPRLVKELPNFMLP
QKVHWRETLPLNPNGKLDRTAIAREIGA
>Mature_507_residues
SQTPDPTPKPLDHLAECGEGDAPALVLRGETLTYEDLRSRVAALASWLQAQVPEEGERIATWAAKGALTCLMPLACARAG
LVHVPINPLLKRAQVAHILADSGALLLLGTAARLKSLEQGDVPAGCTPMDEKAVWEAVEAHEGALKPSDADPDALAAILY
TSGSTGRPKGVMLSHANMWLGAVSVAHYLGMESDDVTLGVLPLSFDYGQNQLLSTWYTGGSVVPLDYLFPNDVVKACAKH
NITTLAAVPPLWVQLTEIEWPSEAVASMRRMTNSGGALTEDLVRDLRAIFPQTRLFPMYGLTEAFRSTYLDPSLVDTHPT
SMGTAIPFAEILVIDDNGEVAQAGEEGELVHCGPLVAQGYWQDAERTAERFKSAPDGSHYGGTAVWSGDRVKRNADGLLY
FVGRRDAMIKSAGNRISPQEIEDAAIATGLVAEAVALGVKDARLGHAVHLVVRAAPEADDPQGKLLPRLVKELPNFMLPQ
KVHWRETLPLNPNGKLDRTAIAREIGA

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=26.5037593984962, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=26.5037593984962, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI38505220, Length=453, Percent_Identity=25.60706401766, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI156151445, Length=521, Percent_Identity=23.4165067178503, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI157311624, Length=484, Percent_Identity=24.5867768595041, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI157311622, Length=484, Percent_Identity=24.5867768595041, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI42544132, Length=501, Percent_Identity=23.75249500998, Blast_Score=106, Evalue=6e-23,
Organism=Homo sapiens, GI58082049, Length=484, Percent_Identity=23.9669421487603, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI122937307, Length=472, Percent_Identity=23.3050847457627, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI115511026, Length=515, Percent_Identity=23.1067961165049, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI195947378, Length=378, Percent_Identity=22.7513227513227, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI40807357, Length=521, Percent_Identity=22.6487523992322, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1788107, Length=535, Percent_Identity=27.2897196261682, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=25.2, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1786801, Length=499, Percent_Identity=26.25250501002, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI221142682, Length=492, Percent_Identity=23.9837398373984, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786810, Length=364, Percent_Identity=25.5494505494505, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790505, Length=546, Percent_Identity=22.3443223443223, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=487, Percent_Identity=26.0780287474333, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=537, Percent_Identity=23.8361266294227, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=490, Percent_Identity=22.8571428571429, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=498, Percent_Identity=22.6907630522088, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71996755, Length=452, Percent_Identity=23.8938053097345, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=371, Percent_Identity=27.4932614555256, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=540, Percent_Identity=23.5185185185185, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=540, Percent_Identity=23.5185185185185, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71994703, Length=540, Percent_Identity=23.5185185185185, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17557194, Length=372, Percent_Identity=24.1935483870968, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=370, Percent_Identity=23.7837837837838, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556356, Length=352, Percent_Identity=24.1477272727273, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=27.7777777777778, Blast_Score=109, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24581924, Length=537, Percent_Identity=24.3947858472998, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI18859661, Length=523, Percent_Identity=24.282982791587, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653035, Length=503, Percent_Identity=24.4532803180915, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648676, Length=468, Percent_Identity=25.6410256410256, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=449, Percent_Identity=22.9398663697105, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130357, Length=401, Percent_Identity=24.4389027431421, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355181, Length=513, Percent_Identity=23.9766081871345, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24648260, Length=290, Percent_Identity=24.8275862068966, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI281365686, Length=291, Percent_Identity=23.7113402061856, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24583465, Length=520, Percent_Identity=20.9615384615385, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583463, Length=520, Percent_Identity=20.9615384615385, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364798, Length=520, Percent_Identity=20.9615384615385, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54510; Mature: 54378

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTPDPTPKPLDHLAECGEGDAPALVLRGETLTYEDLRSRVAALASWLQAQVPEEGERI
CCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
ATWAAKGALTCLMPLACARAGLVHVPINPLLKRAQVAHILADSGALLLLGTAARLKSLEQ
HHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCC
GDVPAGCTPMDEKAVWEAVEAHEGALKPSDADPDALAAILYTSGSTGRPKGVMLSHANMW
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCCCCCCCEEEECCHHH
LGAVSVAHYLGMESDDVTLGVLPLSFDYGQNQLLSTWYTGGSVVPLDYLFPNDVVKACAK
HHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHEECCCCEEEECCCCCHHHHHHHHH
HNITTLAAVPPLWVQLTEIEWPSEAVASMRRMTNSGGALTEDLVRDLRAIFPQTRLFPMY
CCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEHH
GLTEAFRSTYLDPSLVDTHPTSMGTAIPFAEILVIDDNGEVAQAGEEGELVHCGPLVAQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCEEECCHHHHCC
YWQDAERTAERFKSAPDGSHYGGTAVWSGDRVKRNADGLLYFVGRRDAMIKSAGNRISPQ
CHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEECCCCEEEEECCCHHHHHHCCCCCCHH
EIEDAAIATGLVAEAVALGVKDARLGHAVHLVVRAAPEADDPQGKLLPRLVKELPNFMLP
HHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
QKVHWRETLPLNPNGKLDRTAIAREIGA
CCCCCCCCCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SQTPDPTPKPLDHLAECGEGDAPALVLRGETLTYEDLRSRVAALASWLQAQVPEEGERI
CCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
ATWAAKGALTCLMPLACARAGLVHVPINPLLKRAQVAHILADSGALLLLGTAARLKSLEQ
HHHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCC
GDVPAGCTPMDEKAVWEAVEAHEGALKPSDADPDALAAILYTSGSTGRPKGVMLSHANMW
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCCCCCCCEEEECCHHH
LGAVSVAHYLGMESDDVTLGVLPLSFDYGQNQLLSTWYTGGSVVPLDYLFPNDVVKACAK
HHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHEECCCCEEEECCCCCHHHHHHHHH
HNITTLAAVPPLWVQLTEIEWPSEAVASMRRMTNSGGALTEDLVRDLRAIFPQTRLFPMY
CCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEHH
GLTEAFRSTYLDPSLVDTHPTSMGTAIPFAEILVIDDNGEVAQAGEEGELVHCGPLVAQG
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEECCCCCCCEEECCHHHHCC
YWQDAERTAERFKSAPDGSHYGGTAVWSGDRVKRNADGLLYFVGRRDAMIKSAGNRISPQ
CHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCEECCCCEEEEECCCHHHHHHCCCCCCHH
EIEDAAIATGLVAEAVALGVKDARLGHAVHLVVRAAPEADDPQGKLLPRLVKELPNFMLP
HHHHHHHHHHHHHHHHHHCCCHHHHHHEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
QKVHWRETLPLNPNGKLDRTAIAREIGA
CCCCCCCCCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]