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The map label for this gene is fabI1 [H]

Identifier: 85374174

GI number: 85374174

Start: 1393391

End: 1394146

Strand: Reverse

Name: fabI1 [H]

Synonym: ELI_06735

Alternate gene names: 85374174

Gene position: 1394146-1393391 (Counterclockwise)

Preceding gene: 85374175

Following gene: 85374173

Centisome position: 45.67

GC content: 64.81

Gene sequence:

>756_bases
ATGGGCGTCGCCAACGAACGGTCGATCGCCTGGGGCATCGCCAAGGCCTGCGCCGAGGCGGGGGCGGAGCTGGCGTTCAC
CTATCAGGGCGATGCCTTCGGCAAGCGGCTCGAGCCGCTCGCGCAAAGCGTCGGTTCGGACATTATGCTCGATGTCGATG
TCACGGACGATGTCTCCCTGGACAACGCCTTTGCGGAATTGGGGAGGAAGTGGGGCCGGCTCGATTTCGTGATCCACGCC
ATTGCCTTTTCGGACAAGGCGGAACTGACCGGCCGCATTCTCGATACCAGCCGCGAGAACTTCAAGAATTCGCTCGATAT
CTCCGCCTACAGCTTCATCGAGATCGCGCGCCGCGCGCACCCGATGATGGTGGAGCATGGCGGCACGCTGATCACGCTGA
GCTACATGGGCTCCAACCGCGTCACGCCGAACTACAATGTGATGGGCGTCGCCAAGGCGGCGCTGGAATCGGCTGTACGA
TACCTCGCAGACGATCTCGGCCCGGAAGGGATCCGGGTCAATGCGATCAGCCCCGGCCCCATGAAGACGCTGGCCGGGTC
CGCCATCGGCGGCGCGCGCAAGACGTGGAAGCACACCGAGAGCAACGCGCCGCTCCGCGCGAATGCCTCGCTCGAGGCCG
TGGGGGGCACGGCAGTCTATCTGTGCTCGGACGCAGGGGCGTGCACGACGGGCGAGATCATCCGCGTCGATGGCGGTTTC
CACGTGCTCGGCATGCCGCAGCACGAAAACCTCTGA

Upstream 100 bases:

>100_bases
GTTCGGCTTCGGCGGCACCAACGGCACTATGCTGCTGTCGAAGTTTCACGGCTAGGAGGTAGGGATCATGGGCGCACTAG
CGGGAAAACGCGGCCTGGTG

Downstream 100 bases:

>100_bases
CGAGGGCATTATGCGAATACGCGCGACCGATCTTTCCGAACCGGAAGTTCGCGCGCTGCTAGAACTACATCATGCCGAGA
TGACGGCCTATTCGCCGCCG

Product: enoyl-(acyl-carrier-protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 1 [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MGVANERSIAWGIAKACAEAGAELAFTYQGDAFGKRLEPLAQSVGSDIMLDVDVTDDVSLDNAFAELGRKWGRLDFVIHA
IAFSDKAELTGRILDTSRENFKNSLDISAYSFIEIARRAHPMMVEHGGTLITLSYMGSNRVTPNYNVMGVAKAALESAVR
YLADDLGPEGIRVNAISPGPMKTLAGSAIGGARKTWKHTESNAPLRANASLEAVGGTAVYLCSDAGACTTGEIIRVDGGF
HVLGMPQHENL

Sequences:

>Translated_251_residues
MGVANERSIAWGIAKACAEAGAELAFTYQGDAFGKRLEPLAQSVGSDIMLDVDVTDDVSLDNAFAELGRKWGRLDFVIHA
IAFSDKAELTGRILDTSRENFKNSLDISAYSFIEIARRAHPMMVEHGGTLITLSYMGSNRVTPNYNVMGVAKAALESAVR
YLADDLGPEGIRVNAISPGPMKTLAGSAIGGARKTWKHTESNAPLRANASLEAVGGTAVYLCSDAGACTTGEIIRVDGGF
HVLGMPQHENL
>Mature_250_residues
GVANERSIAWGIAKACAEAGAELAFTYQGDAFGKRLEPLAQSVGSDIMLDVDVTDDVSLDNAFAELGRKWGRLDFVIHAI
AFSDKAELTGRILDTSRENFKNSLDISAYSFIEIARRAHPMMVEHGGTLITLSYMGSNRVTPNYNVMGVAKAALESAVRY
LADDLGPEGIRVNAISPGPMKTLAGSAIGGARKTWKHTESNAPLRANASLEAVGGTAVYLCSDAGACTTGEIIRVDGGFH
VLGMPQHENL

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=171, Percent_Identity=28.0701754385965, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI59889578, Length=233, Percent_Identity=26.1802575107296, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI10190704, Length=196, Percent_Identity=26.530612244898, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI32483357, Length=156, Percent_Identity=27.5641025641026, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI126723750, Length=175, Percent_Identity=26.8571428571429, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787545, Length=249, Percent_Identity=48.995983935743, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1787905, Length=233, Percent_Identity=26.6094420600858, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788459, Length=250, Percent_Identity=28, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367365, Length=240, Percent_Identity=30, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1789378, Length=238, Percent_Identity=24.7899159663866, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=183, Percent_Identity=28.4153005464481, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17559104, Length=148, Percent_Identity=33.1081081081081, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=240, Percent_Identity=24.1666666666667, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=137, Percent_Identity=28.4671532846715, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=119, Percent_Identity=31.9327731092437, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6324126, Length=194, Percent_Identity=27.8350515463918, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571526, Length=198, Percent_Identity=26.7676767676768, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21357041, Length=207, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI23397609, Length=239, Percent_Identity=25.1046025104602, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644339, Length=195, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644337, Length=179, Percent_Identity=26.2569832402235, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 26690; Mature: 26558

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVANERSIAWGIAKACAEAGAELAFTYQGDAFGKRLEPLAQSVGSDIMLDVDVTDDVSL
CCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCH
DNAFAELGRKWGRLDFVIHAIAFSDKAELTGRILDTSRENFKNSLDISAYSFIEIARRAH
HHHHHHHHHHCCCHHHEEEEHHCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCC
PMMVEHGGTLITLSYMGSNRVTPNYNVMGVAKAALESAVRYLADDLGPEGIRVNAISPGP
CEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
MKTLAGSAIGGARKTWKHTESNAPLRANASLEAVGGTAVYLCSDAGACTTGEIIRVDGGF
HHHHHCCCCCCCHHHHCCCCCCCCEEECCCEEECCCEEEEEECCCCCCCCCCEEEECCCE
HVLGMPQHENL
EEEECCCCCCC
>Mature Secondary Structure 
GVANERSIAWGIAKACAEAGAELAFTYQGDAFGKRLEPLAQSVGSDIMLDVDVTDDVSL
CCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCH
DNAFAELGRKWGRLDFVIHAIAFSDKAELTGRILDTSRENFKNSLDISAYSFIEIARRAH
HHHHHHHHHHCCCHHHEEEEHHCCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHCC
PMMVEHGGTLITLSYMGSNRVTPNYNVMGVAKAALESAVRYLADDLGPEGIRVNAISPGP
CEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
MKTLAGSAIGGARKTWKHTESNAPLRANASLEAVGGTAVYLCSDAGACTTGEIIRVDGGF
HHHHHCCCCCCCHHHHCCCCCCCCEEECCCEEECCCEEEEEECCCCCCCCCCEEEECCCE
HVLGMPQHENL
EEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]