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The map label for this gene is hemN [H]

Identifier: 85374027

GI number: 85374027

Start: 1241633

End: 1242784

Strand: Reverse

Name: hemN [H]

Synonym: ELI_06000

Alternate gene names: 85374027

Gene position: 1242784-1241633 (Counterclockwise)

Preceding gene: 85374028

Following gene: 85374026

Centisome position: 40.72

GC content: 62.33

Gene sequence:

>1152_bases
ATGGCCCGCGCGCTCTACATCCATTGGCCCTTCTGCGCGAAGAAGTGCCCCTATTGCGATTTCAACAGCCACGTGCGCGC
CAGCGTCGATCACGCACTGTGGGAGCGCGCGATGCTGGCCGATATGCGCTACGAGGCGGGGAAAGCCGGTGGAGAGGCGC
TGACCTCCATTTTCTTCGGCGGGGGCACACCGTCGCTTATGCCGCCTGCTCTGGTTGCTTCGCTGTTGCAGCAGGCCGAG
CGGCTCTGGGGCTTCGCGCCCGATATCGAGATCACGCTCGAAGCCAATCCCTCGTCGGTAGAAACGGCGAATTTTCATGC
CCTGGCTGCGGCGGGTGTCAATCGCGTGTCGCTGGGCGTACAATCGCTGGACGATGCGGCTCTCCGCTTCCTCGGACGCT
TGCACGGCGTCGAAGAGGCGCTGACTGCTTTGGAAACCGCGCAGAAAGCCTTCCAGCGCGTCAGCTTCGACCTGATTTAC
GCCCTGCCCGACCAGACCGAGAGCGATTGGCGCGCCGGACTTGAGCGGGCGCTATCCTTCGGCACGTCACACCTGTCTCT
TTACCAACTGACGATCGAGCCCGGGACGCGCTTTGCGACCGATGTCAGGCGGGGAGTCTTCGATCCGCTGGCGGATGATC
CGGCTGCCGATCTGTACACAGTGACACAGGCGGTCACCGCGAGTGCAGGCCTTCCGGCTTATGAAATCAGCAATCACGCC
AGGCCGGGTGAGGAAAGCCGTCACAACCTCACGTACTGGCGTTACCACGATTATTGCGGGATCGGTCCCGGCGCACATGG
GCGCCGCGGCGGTTTCGCAACCCTGCGACACAAAAAGCCCGAGAACTGGCTGGCGGCGGTCGATGAGTGCGGCAGCGGAG
CGCAAGAGGAGCGTCGCCTTTCACCCGCTGAGCAAGCGTCAGAAGCATTGGTCATGGGCTTGAGGCTCGCTGCAGGAGTC
GATCTCGAGACTCTATCGGCACGCTTCGGCATCGGCGAAGTTTCTTTGATCGATCCCGATAAGCGACAATTGTATTCCCA
GCTCGGCCTGATCGAGCACGTAAACGAGCGATTGCGCGTGACGGCCAAGGGCATGCCGCTGCTCGATGCGCTTCTGGGTG
AGTTGGTCTCGAGCGAACTGGTGCTGGCGTGA

Upstream 100 bases:

>100_bases
ATTCGCCGAAATCGACCCGGAAGAGAAGCACGCCATCAGCCATCGCGCCGATGCTTTCGCCAAGCTGGTCGCGAAACAGT
TTGGCAGCTAGACTGCGGCC

Downstream 100 bases:

>100_bases
GCGCCGCTGAATTACTCGCGCAATGGCACACTTATCTCGCCGACGAGAGACGACGTTCGCCCCATACCGTGCGGGCCTAT
CACACGGCGGCGCGCCGAAT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MARALYIHWPFCAKKCPYCDFNSHVRASVDHALWERAMLADMRYEAGKAGGEALTSIFFGGGTPSLMPPALVASLLQQAE
RLWGFAPDIEITLEANPSSVETANFHALAAAGVNRVSLGVQSLDDAALRFLGRLHGVEEALTALETAQKAFQRVSFDLIY
ALPDQTESDWRAGLERALSFGTSHLSLYQLTIEPGTRFATDVRRGVFDPLADDPAADLYTVTQAVTASAGLPAYEISNHA
RPGEESRHNLTYWRYHDYCGIGPGAHGRRGGFATLRHKKPENWLAAVDECGSGAQEERRLSPAEQASEALVMGLRLAAGV
DLETLSARFGIGEVSLIDPDKRQLYSQLGLIEHVNERLRVTAKGMPLLDALLGELVSSELVLA

Sequences:

>Translated_383_residues
MARALYIHWPFCAKKCPYCDFNSHVRASVDHALWERAMLADMRYEAGKAGGEALTSIFFGGGTPSLMPPALVASLLQQAE
RLWGFAPDIEITLEANPSSVETANFHALAAAGVNRVSLGVQSLDDAALRFLGRLHGVEEALTALETAQKAFQRVSFDLIY
ALPDQTESDWRAGLERALSFGTSHLSLYQLTIEPGTRFATDVRRGVFDPLADDPAADLYTVTQAVTASAGLPAYEISNHA
RPGEESRHNLTYWRYHDYCGIGPGAHGRRGGFATLRHKKPENWLAAVDECGSGAQEERRLSPAEQASEALVMGLRLAAGV
DLETLSARFGIGEVSLIDPDKRQLYSQLGLIEHVNERLRVTAKGMPLLDALLGELVSSELVLA
>Mature_382_residues
ARALYIHWPFCAKKCPYCDFNSHVRASVDHALWERAMLADMRYEAGKAGGEALTSIFFGGGTPSLMPPALVASLLQQAER
LWGFAPDIEITLEANPSSVETANFHALAAAGVNRVSLGVQSLDDAALRFLGRLHGVEEALTALETAQKAFQRVSFDLIYA
LPDQTESDWRAGLERALSFGTSHLSLYQLTIEPGTRFATDVRRGVFDPLADDPAADLYTVTQAVTASAGLPAYEISNHAR
PGEESRHNLTYWRYHDYCGIGPGAHGRRGGFATLRHKKPENWLAAVDECGSGAQEERRLSPAEQASEALVMGLRLAAGVD
LETLSARFGIGEVSLIDPDKRQLYSQLGLIEHVNERLRVTAKGMPLLDALLGELVSSELVLA

Specific function: Anaerobic transformation of coproporphyrinogen-III into protoporphyrinogen-IX [H]

COG id: COG0635

COG function: function code H; Coproporphyrinogen III oxidase and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anaerobic coproporphyrinogen-III oxidase family [H]

Homologues:

Organism=Homo sapiens, GI8922911, Length=302, Percent_Identity=41.0596026490066, Blast_Score=228, Evalue=5e-60,
Organism=Escherichia coli, GI1789325, Length=376, Percent_Identity=35.1063829787234, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI87082341, Length=381, Percent_Identity=24.4094488188976, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR004559
- InterPro:   IPR010723
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06969 HemN_C; PF04055 Radical_SAM [H]

EC number: =1.3.99.22 [H]

Molecular weight: Translated: 41688; Mature: 41556

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARALYIHWPFCAKKCPYCDFNSHVRASVDHALWERAMLADMRYEAGKAGGEALTSIFFG
CCCEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GGTPSLMPPALVASLLQQAERLWGFAPDIEITLEANPSSVETANFHALAAAGVNRVSLGV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHH
QSLDDAALRFLGRLHGVEEALTALETAQKAFQRVSFDLIYALPDQTESDWRAGLERALSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHH
GTSHLSLYQLTIEPGTRFATDVRRGVFDPLADDPAADLYTVTQAVTASAGLPAYEISNHA
CCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCC
RPGEESRHNLTYWRYHDYCGIGPGAHGRRGGFATLRHKKPENWLAAVDECGSGAQEERRL
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHCC
SPAEQASEALVMGLRLAAGVDLETLSARFGIGEVSLIDPDKRQLYSQLGLIEHVNERLRV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHEE
TAKGMPLLDALLGELVSSELVLA
EECCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARALYIHWPFCAKKCPYCDFNSHVRASVDHALWERAMLADMRYEAGKAGGEALTSIFFG
CCEEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCC
GGTPSLMPPALVASLLQQAERLWGFAPDIEITLEANPSSVETANFHALAAAGVNRVSLGV
CCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCHHHHHHH
QSLDDAALRFLGRLHGVEEALTALETAQKAFQRVSFDLIYALPDQTESDWRAGLERALSF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHH
GTSHLSLYQLTIEPGTRFATDVRRGVFDPLADDPAADLYTVTQAVTASAGLPAYEISNHA
CCCCEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHCCCC
RPGEESRHNLTYWRYHDYCGIGPGAHGRRGGFATLRHKKPENWLAAVDECGSGAQEERRL
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHCC
SPAEQASEALVMGLRLAAGVDLETLSARFGIGEVSLIDPDKRQLYSQLGLIEHVNERLRV
CHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHEE
TAKGMPLLDALLGELVSSELVLA
EECCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8757728; 8969508; 9384377; 1339421 [H]