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The map label for this gene is tyrS [H]

Identifier: 85374010

GI number: 85374010

Start: 1223387

End: 1224616

Strand: Reverse

Name: tyrS [H]

Synonym: ELI_05915

Alternate gene names: 85374010

Gene position: 1224616-1223387 (Counterclockwise)

Preceding gene: 85374013

Following gene: 85374009

Centisome position: 40.12

GC content: 60.89

Gene sequence:

>1230_bases
ATGAGCGAATTCGAATCCGACCTCCTGCAGCTGCTTTCAGAACGCGGCCACTTGCACCAGATCACCGATGCCGGTGCGCT
CGACGCGTTGGCGATGAAAGAGGTCGTGACCGGTTATGTCGGCTTCGACCCCACCGCACCCTCGCTCCATATCGGCAATC
TCGCCTCGATCATGCTGCTGAAGCGATTGCAGCAGGCCGGGCATCGCCCGATCGTCATCATGGGCGGCGGCACGGCCAAG
GTGGGTGACCCCTCGGGTCGGGACGAAACCCGCACGATGCTGACCGACGAGAAGCTCGCCGGCAATATCGCTTCGATCAA
GGGTAGTTTCGAACAGATCCTGCGCTTCGACGACAGCGAGACCGGCGCGCTGCTGATAAACAACGACGATTGGCTGAACG
AGCTCGGTTATATCGAGCTGCTGCGCGATGTCGGGCCGCATTTCACTATCAACCGCATGCTGACATTCGACTCGGTCAAG
CTGCGGCTCGATCGCGAGCAGCCGCTGACCTTCCTCGAATTCAATTACATGATCATGCAGGCCTACGATTATCTCGTGCT
CAACCGCGAATATGGGTGCCGGTTGCAGCTGGGTGGCAGCGACCAGTGGGGCAATATCGTCAACGGTATCGAGCTATGTC
GCCGCATCGACGGCAAGGAAGTCTATGGCGTCACCGCACCGCTGATTACGCGTGCCGATGGCGCGAAAATGGGCAAGTCG
GTCGACGGTGCCGTGTGGCTCAATGAAGCGCAACTGCCCGCTTATGATTTCTGGCAATACTGGCGGAACACCGATGACCG
AGACGTCGGCAGGTTCCTGCGGCTGTTTACCGACCTGCCGCTCGACGAGATTGCACGGCTGGAACAGCTCGAAGGTGCCG
CAATCAACGACGCCAAAATCGTACTCGCCAACGAAGTGACGGCAATGGTACGCGGTGCAGACGCTGCCAGGTCCGCCGAG
GCGACTGCCGCGCAGACCTTTGCCGGCGGCGGTGCGGGCGAAGACCTACCGACTCTCACGCTTGGCGAGGAGGAAATGTC
CTTGCCTGCCGTCCTGACGGCGATCGGGTTCACCAGGTCCAATGGCGAGGCCAAGCGCAAGATTGCCGAAGGCGCAGTCA
AGCTCGATGGCGAGGCCACACGCGATCCCGGCGCCATGATCGCGCTTGCCGAAGGCGCGGAGATCAAGATCAGCCTGGGC
AAGAAACGGCACGGCCTGCTTGTTCGCTAA

Upstream 100 bases:

>100_bases
ATGGAGGACAAGCTCATGCATGATCGCCGCTATTGCCCAGTGCAGCGATGCCCGCAAGCCACTTGGAGCCGCCTCTCCCC
GCCGCTATAGGCCGCGCCCT

Downstream 100 bases:

>100_bases
ACCGCGCAACCCCGCAGAAACGCTGGGTTTTGTGCCAAATCGGGACAGCGCAGCTTAACCTAATTTCAGGCAATTGGCGG
CATAAGTGCCCTCCACACCA

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MSEFESDLLQLLSERGHLHQITDAGALDALAMKEVVTGYVGFDPTAPSLHIGNLASIMLLKRLQQAGHRPIVIMGGGTAK
VGDPSGRDETRTMLTDEKLAGNIASIKGSFEQILRFDDSETGALLINNDDWLNELGYIELLRDVGPHFTINRMLTFDSVK
LRLDREQPLTFLEFNYMIMQAYDYLVLNREYGCRLQLGGSDQWGNIVNGIELCRRIDGKEVYGVTAPLITRADGAKMGKS
VDGAVWLNEAQLPAYDFWQYWRNTDDRDVGRFLRLFTDLPLDEIARLEQLEGAAINDAKIVLANEVTAMVRGADAARSAE
ATAAQTFAGGGAGEDLPTLTLGEEEMSLPAVLTAIGFTRSNGEAKRKIAEGAVKLDGEATRDPGAMIALAEGAEIKISLG
KKRHGLLVR

Sequences:

>Translated_409_residues
MSEFESDLLQLLSERGHLHQITDAGALDALAMKEVVTGYVGFDPTAPSLHIGNLASIMLLKRLQQAGHRPIVIMGGGTAK
VGDPSGRDETRTMLTDEKLAGNIASIKGSFEQILRFDDSETGALLINNDDWLNELGYIELLRDVGPHFTINRMLTFDSVK
LRLDREQPLTFLEFNYMIMQAYDYLVLNREYGCRLQLGGSDQWGNIVNGIELCRRIDGKEVYGVTAPLITRADGAKMGKS
VDGAVWLNEAQLPAYDFWQYWRNTDDRDVGRFLRLFTDLPLDEIARLEQLEGAAINDAKIVLANEVTAMVRGADAARSAE
ATAAQTFAGGGAGEDLPTLTLGEEEMSLPAVLTAIGFTRSNGEAKRKIAEGAVKLDGEATRDPGAMIALAEGAEIKISLG
KKRHGLLVR
>Mature_408_residues
SEFESDLLQLLSERGHLHQITDAGALDALAMKEVVTGYVGFDPTAPSLHIGNLASIMLLKRLQQAGHRPIVIMGGGTAKV
GDPSGRDETRTMLTDEKLAGNIASIKGSFEQILRFDDSETGALLINNDDWLNELGYIELLRDVGPHFTINRMLTFDSVKL
RLDREQPLTFLEFNYMIMQAYDYLVLNREYGCRLQLGGSDQWGNIVNGIELCRRIDGKEVYGVTAPLITRADGAKMGKSV
DGAVWLNEAQLPAYDFWQYWRNTDDRDVGRFLRLFTDLPLDEIARLEQLEGAAINDAKIVLANEVTAMVRGADAARSAEA
TAAQTFAGGGAGEDLPTLTLGEEEMSLPAVLTAIGFTRSNGEAKRKIAEGAVKLDGEATRDPGAMIALAEGAEIKISLGK
KRHGLLVR

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=293, Percent_Identity=40.6143344709898, Blast_Score=212, Evalue=5e-55,
Organism=Escherichia coli, GI1787925, Length=392, Percent_Identity=39.7959183673469, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI25145802, Length=397, Percent_Identity=31.2342569269521, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25145805, Length=325, Percent_Identity=28, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6325160, Length=409, Percent_Identity=32.518337408313, Blast_Score=211, Evalue=2e-55,
Organism=Drosophila melanogaster, GI20130383, Length=363, Percent_Identity=36.3636363636364, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44712; Mature: 44581

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFESDLLQLLSERGHLHQITDAGALDALAMKEVVTGYVGFDPTAPSLHIGNLASIMLL
CCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHH
KRLQQAGHRPIVIMGGGTAKVGDPSGRDETRTMLTDEKLAGNIASIKGSFEQILRFDDSE
HHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHCCHHHHHHHHHHHHHCCCCC
TGALLINNDDWLNELGYIELLRDVGPHFTINRMLTFDSVKLRLDREQPLTFLEFNYMIMQ
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCEEEEHHHHHHH
AYDYLVLNREYGCRLQLGGSDQWGNIVNGIELCRRIDGKEVYGVTAPLITRADGAKMGKS
HHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCHHEECCCCHHCCCC
VDGAVWLNEAQLPAYDFWQYWRNTDDRDVGRFLRLFTDLPLDEIARLEQLEGAAINDAKI
CCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEE
VLANEVTAMVRGADAARSAEATAAQTFAGGGAGEDLPTLTLGEEEMSLPAVLTAIGFTRS
EEEHHHHHHHHCCCHHHCCHHHHHHHHCCCCCCCCCCEEEECCHHHCHHHHHHHHCCCCC
NGEAKRKIAEGAVKLDGEATRDPGAMIALAEGAEIKISLGKKRHGLLVR
CCHHHHHHHCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
SEFESDLLQLLSERGHLHQITDAGALDALAMKEVVTGYVGFDPTAPSLHIGNLASIMLL
CHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHH
KRLQQAGHRPIVIMGGGTAKVGDPSGRDETRTMLTDEKLAGNIASIKGSFEQILRFDDSE
HHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHCHHHHHCCHHHHHHHHHHHHHCCCCC
TGALLINNDDWLNELGYIELLRDVGPHFTINRMLTFDSVKLRLDREQPLTFLEFNYMIMQ
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEEEEECCCCCCEEEEHHHHHHH
AYDYLVLNREYGCRLQLGGSDQWGNIVNGIELCRRIDGKEVYGVTAPLITRADGAKMGKS
HHCEEEEECCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCHHEECCCCHHCCCC
VDGAVWLNEAQLPAYDFWQYWRNTDDRDVGRFLRLFTDLPLDEIARLEQLEGAAINDAKI
CCCEEEEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCEE
VLANEVTAMVRGADAARSAEATAAQTFAGGGAGEDLPTLTLGEEEMSLPAVLTAIGFTRS
EEEHHHHHHHHCCCHHHCCHHHHHHHHCCCCCCCCCCEEEECCHHHCHHHHHHHHCCCCC
NGEAKRKIAEGAVKLDGEATRDPGAMIALAEGAEIKISLGKKRHGLLVR
CCHHHHHHHCCCEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA