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The map label for this gene is pth

Identifier: 85373994

GI number: 85373994

Start: 1204251

End: 1204820

Strand: Reverse

Name: pth

Synonym: ELI_05835

Alternate gene names: 85373994

Gene position: 1204820-1204251 (Counterclockwise)

Preceding gene: 85373995

Following gene: 85373993

Centisome position: 39.47

GC content: 62.11

Gene sequence:

>570_bases
ATGCAAATTTGGGCAGGCCTCGGAAATCCCGGACCGAAATACGCGCTGCACCGGCACAATGTCGGGTTCATGGCGTGCGA
TGTCATCGCCGAGATGCACGGGTTCGAGCCGGTGCAGAAAAAGTTCCAGGGCTGGGTCCAAGAGGGCCGCATCGGCTCGG
AGAAGGTGCTCCTGCTCAAGCCCGGCACTTTCATGAACGAAAGCGGTCGCGCGATCGGTGAAGCCATGCGCTTCTATAAG
CTCGAACCGCAGGACGTGACGGTCTTCTACGACGAGCTCGACCTCGAACCCTTCAAGATCAAGGTGAAACGCGGCGGCGG
GGCGGCAGGGCATAACGGCATCCGCTCGACGATCCGCCATATCGGCGAGGACTTTCGCCGTATCCGGATCGGTATCGGCC
ATCCGGGCCACAAGGACCGGGTCACCGGCCATGTGCTCGGCAATTATGCCAAGGCGGAACAGGACGATCTCGTGCAGATG
CTCGGCGCGATCGGCGCGGAGGCCGAATGGCTGGCCAAGGGTGATGATGCGCGCTTCATGAGCGACATCGCGCTGCGACT
GCAGGCGTGA

Upstream 100 bases:

>100_bases
GACTAAGCCCGCGCTTCATCGCACGCACATCACCAAGGCGCCGGGCTGGGAGACCAGTCCGGCGTTTTCCTTTTTGCGTG
AGCCGCACTAGAGGGACGCG

Downstream 100 bases:

>100_bases
ACCGGAGCCTCGTTCTTTTCATCGGATTGGCCTGTGCGGCCTGCGCGATTTCGCAACCTTCCAAACAGATCGACCTTGCG
CGCTTCCAGTCCGAACGCCC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MQIWAGLGNPGPKYALHRHNVGFMACDVIAEMHGFEPVQKKFQGWVQEGRIGSEKVLLLKPGTFMNESGRAIGEAMRFYK
LEPQDVTVFYDELDLEPFKIKVKRGGGAAGHNGIRSTIRHIGEDFRRIRIGIGHPGHKDRVTGHVLGNYAKAEQDDLVQM
LGAIGAEAEWLAKGDDARFMSDIALRLQA

Sequences:

>Translated_189_residues
MQIWAGLGNPGPKYALHRHNVGFMACDVIAEMHGFEPVQKKFQGWVQEGRIGSEKVLLLKPGTFMNESGRAIGEAMRFYK
LEPQDVTVFYDELDLEPFKIKVKRGGGAAGHNGIRSTIRHIGEDFRRIRIGIGHPGHKDRVTGHVLGNYAKAEQDDLVQM
LGAIGAEAEWLAKGDDARFMSDIALRLQA
>Mature_189_residues
MQIWAGLGNPGPKYALHRHNVGFMACDVIAEMHGFEPVQKKFQGWVQEGRIGSEKVLLLKPGTFMNESGRAIGEAMRFYK
LEPQDVTVFYDELDLEPFKIKVKRGGGAAGHNGIRSTIRHIGEDFRRIRIGIGHPGHKDRVTGHVLGNYAKAEQDDLVQM
LGAIGAEAEWLAKGDDARFMSDIALRLQA

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=167, Percent_Identity=34.1317365269461, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1787455, Length=157, Percent_Identity=43.312101910828, Blast_Score=110, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_ERYLH (Q2NAN2)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458056.1
- ProteinModelPortal:   Q2NAN2
- SMR:   Q2NAN2
- STRING:   Q2NAN2
- GeneID:   3870874
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05835
- NMPDR:   fig|314225.3.peg.1897
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q2NAN2
- ProtClustDB:   PRK05426
- BioCyc:   ELIT314225:ELI_05835-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20994; Mature: 20994

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIWAGLGNPGPKYALHRHNVGFMACDVIAEMHGFEPVQKKFQGWVQEGRIGSEKVLLLK
CEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEC
PGTFMNESGRAIGEAMRFYKLEPQDVTVFYDELDLEPFKIKVKRGGGAAGHNGIRSTIRH
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHH
IGEDFRRIRIGIGHPGHKDRVTGHVLGNYAKAEQDDLVQMLGAIGAEAEWLAKGDDARFM
HHHHHHEEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHH
SDIALRLQA
HHHHHHCCC
>Mature Secondary Structure
MQIWAGLGNPGPKYALHRHNVGFMACDVIAEMHGFEPVQKKFQGWVQEGRIGSEKVLLLK
CEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEC
PGTFMNESGRAIGEAMRFYKLEPQDVTVFYDELDLEPFKIKVKRGGGAAGHNGIRSTIRH
CCCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHHHHHH
IGEDFRRIRIGIGHPGHKDRVTGHVLGNYAKAEQDDLVQMLGAIGAEAEWLAKGDDARFM
HHHHHHEEEEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHH
SDIALRLQA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA