Click here to switch to the map view.

The map label for this gene is eno

Identifier: 85373975

GI number: 85373975

Start: 1184592

End: 1185878

Strand: Direct

Name: eno

Synonym: ELI_05740

Alternate gene names: 85373975

Gene position: 1184592-1185878 (Clockwise)

Preceding gene: 85373967

Following gene: 85373977

Centisome position: 38.81

GC content: 63.09

Gene sequence:

>1287_bases
ATGACCGCCATCATCGACCTGCATGCCCGCGAGATCCTCGATAGCCGGGGCAATCCGACCGTCGAAGTGGACGTGCTTCT
CGAAGACGGCAGCTTCGGGCGCGCCGCGGTGCCGTCGGGCGCTTCGACCGGCGCGCATGAAGCGATCGAGCTGCGCGATG
GCGATCAGGGGCGTTATCTCGGCAAGGGCGTCACAAGGGCGGTCGATGCGGTCAACACGACGATCGCCGACACGCTGCTG
GGCCTCGACGCCGAAGATCAACGCGATATCGATACAGTGATGCTCGATCTCGACGGTACGCCTAACAAGGCGAAGCTTGG
CGCGAATGCTATCCTCGGCACCAGCCTGGCGGTCGCCAAGGCTGCGGCGGCTGCGCGTGGCATGCCGCTGTGGGCCTATG
TCGGCGGCGTCTCCGCGCACATGCTGCCGGTCCCGATGATGAATATCGTCAACGGCGGCGAGCATGCCGACAATCCGATC
GATATCCAGGAATTCATGGTCATGCCGGTCGGCGCGGACAGCCTTGCCGAAGCGGTGCGCTGGGGCGCGGAGATTTTCCA
CACGCTCAAGAAGGGCTTGTCGGAAAAGGGCCTGTCGACCTCGGTGGGCGACGAAGGCGGCTTTGCTCCCGACATCGCCA
GTACGCGCGACGCGCTCGACTTCATCATGCAGTCGATCGAGAAAGCGGGCTTCAAGCCGGGCGAGGAAGTGGCGCTTGCC
CTCGATTGCGCGTCGACGGAATTCTTCGCCGATGGCCGCTATGACCTGGCGGGGGAAGAAGTATCGCTTTCGCCCGAGGA
AATGGCGAAATACCTCGCGGACCTGTGCAACGACTACCCGATCCGTTCGATCGAAGACGGCATGGCGGAGGACGATCTCG
AAGGCTGGAAAGCGCTGACCGACCTGATTGGCAACAAGGTCCAATTGGTGGGCGACGACCTGTTCGTCACCAATTCGGAG
CGTCTCGCCATGGGTATCGACAAGGGACTCGCCAATTCGCTGCTGGTCAAGGTCAACCAGATCGGCACGCTCAGCGAGAC
GCTGGAAGCCGTCGATATGGCGCACCGTGCCGGCTACACCTGTGTGATGAGCCACCGCTCGGGCGAAACCGAGGATGCGA
CCATCGCAGACTTGGCCGTCGCCACCAATTGCGGGCAGATCAAGACCGGCTCGCTGGCGCGGTCGGACAGGCTGGCCAAA
TACAACCAGCTTATCCGGATCGAGGAAGAGCTGGGCAACAGCGCGCATTATGCCGGTGCGGCGTGCTTTGGTCGTCTGGC
CCGCTAG

Upstream 100 bases:

>100_bases
TCGGTTCCGCCGCATATTGCGCCCGCATCCCTCGATATGGGGGTGTCTTAGCCTAATACAACACATACAGACTTCCGTCC
AGCAACCGGAGCCATACCCC

Downstream 100 bases:

>100_bases
GTTCTAGTTTGGAGGTGCGAACTGCGGCCGCTTCGTGGGGCGCATGATCGCGGCTTCGGGATGGGCGGCCGCAATATGCA
CGTCCATCCGATTGACCTGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTAIIDLHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAIELRDGDQGRYLGKGVTRAVDAVNTTIADTLL
GLDAEDQRDIDTVMLDLDGTPNKAKLGANAILGTSLAVAKAAAAARGMPLWAYVGGVSAHMLPVPMMNIVNGGEHADNPI
DIQEFMVMPVGADSLAEAVRWGAEIFHTLKKGLSEKGLSTSVGDEGGFAPDIASTRDALDFIMQSIEKAGFKPGEEVALA
LDCASTEFFADGRYDLAGEEVSLSPEEMAKYLADLCNDYPIRSIEDGMAEDDLEGWKALTDLIGNKVQLVGDDLFVTNSE
RLAMGIDKGLANSLLVKVNQIGTLSETLEAVDMAHRAGYTCVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGNSAHYAGAACFGRLAR

Sequences:

>Translated_428_residues
MTAIIDLHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAIELRDGDQGRYLGKGVTRAVDAVNTTIADTLL
GLDAEDQRDIDTVMLDLDGTPNKAKLGANAILGTSLAVAKAAAAARGMPLWAYVGGVSAHMLPVPMMNIVNGGEHADNPI
DIQEFMVMPVGADSLAEAVRWGAEIFHTLKKGLSEKGLSTSVGDEGGFAPDIASTRDALDFIMQSIEKAGFKPGEEVALA
LDCASTEFFADGRYDLAGEEVSLSPEEMAKYLADLCNDYPIRSIEDGMAEDDLEGWKALTDLIGNKVQLVGDDLFVTNSE
RLAMGIDKGLANSLLVKVNQIGTLSETLEAVDMAHRAGYTCVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGNSAHYAGAACFGRLAR
>Mature_427_residues
TAIIDLHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAIELRDGDQGRYLGKGVTRAVDAVNTTIADTLLG
LDAEDQRDIDTVMLDLDGTPNKAKLGANAILGTSLAVAKAAAAARGMPLWAYVGGVSAHMLPVPMMNIVNGGEHADNPID
IQEFMVMPVGADSLAEAVRWGAEIFHTLKKGLSEKGLSTSVGDEGGFAPDIASTRDALDFIMQSIEKAGFKPGEEVALAL
DCASTEFFADGRYDLAGEEVSLSPEEMAKYLADLCNDYPIRSIEDGMAEDDLEGWKALTDLIGNKVQLVGDDLFVTNSER
LAMGIDKGLANSLLVKVNQIGTLSETLEAVDMAHRAGYTCVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEELGNSAHYAGAACFGRLAR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=433, Percent_Identity=51.270207852194, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=438, Percent_Identity=49.7716894977169, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897477, Length=429, Percent_Identity=49.6503496503497, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=429, Percent_Identity=49.6503496503497, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=45.6674473067916, Blast_Score=357, Evalue=1e-98,
Organism=Homo sapiens, GI169201331, Length=336, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI169201757, Length=336, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI239744207, Length=336, Percent_Identity=26.1904761904762, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=60.1415094339623, Blast_Score=507, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=51.5151515151515, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=51.5151515151515, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=47.3684210526316, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=50.2314814814815, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=50.2314814814815, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=50.2314814814815, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=49.6535796766744, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=50.3464203233256, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=49.5327102803738, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=49.5327102803738, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=49.5327102803738, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=49.5327102803738, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=49.5327102803738, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=49.5327102803738, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ERYLH (Q2NAQ1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458037.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2NAQ1
- SMR:   Q2NAQ1
- STRING:   Q2NAQ1
- GeneID:   3870835
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05740
- NMPDR:   fig|314225.3.peg.1917
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q2NAQ1
- ProtClustDB:   PRK00077
- BioCyc:   ELIT314225:ELI_05740-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45319; Mature: 45188

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDLHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAIELRDGDQGRYL
CCEEEEHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
GKGVTRAVDAVNTTIADTLLGLDAEDQRDIDTVMLDLDGTPNKAKLGANAILGTSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAAAARGMPLWAYVGGVSAHMLPVPMMNIVNGGEHADNPIDIQEFMVMPVGADSLAEAVR
HHHHHCCCCCEEECCCCCHHCCCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
WGAEIFHTLKKGLSEKGLSTSVGDEGGFAPDIASTRDALDFIMQSIEKAGFKPGEEVALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCASTEFFADGRYDLAGEEVSLSPEEMAKYLADLCNDYPIRSIEDGMAEDDLEGWKALT
EEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHH
DLIGNKVQLVGDDLFVTNSERLAMGIDKGLANSLLVKVNQIGTLSETLEAVDMAHRAGYT
HHHCCEEEEEECEEEEECCCHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
CVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGNSAHYAGA
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ACFGRLAR
HHHHHHCC
>Mature Secondary Structure 
TAIIDLHAREILDSRGNPTVEVDVLLEDGSFGRAAVPSGASTGAHEAIELRDGDQGRYL
CEEEEHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
GKGVTRAVDAVNTTIADTLLGLDAEDQRDIDTVMLDLDGTPNKAKLGANAILGTSLAVAK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCCHHHHHHHHHHH
AAAAARGMPLWAYVGGVSAHMLPVPMMNIVNGGEHADNPIDIQEFMVMPVGADSLAEAVR
HHHHHCCCCCEEECCCCCHHCCCCCHHHHHCCCCCCCCCCCHHHHEEECCCHHHHHHHHH
WGAEIFHTLKKGLSEKGLSTSVGDEGGFAPDIASTRDALDFIMQSIEKAGFKPGEEVALA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
LDCASTEFFADGRYDLAGEEVSLSPEEMAKYLADLCNDYPIRSIEDGMAEDDLEGWKALT
EEECCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHH
DLIGNKVQLVGDDLFVTNSERLAMGIDKGLANSLLVKVNQIGTLSETLEAVDMAHRAGYT
HHHCCEEEEEECEEEEECCCHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCE
CVMSHRSGETEDATIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGNSAHYAGA
EEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
ACFGRLAR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA