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The map label for this gene is greA

Identifier: 85373967

GI number: 85373967

Start: 1181176

End: 1181643

Strand: Direct

Name: greA

Synonym: ELI_05700

Alternate gene names: 85373967

Gene position: 1181176-1181643 (Clockwise)

Preceding gene: 85373966

Following gene: 85373975

Centisome position: 38.7

GC content: 60.9

Gene sequence:

>468_bases
ATGGAAAAGGTTCCGATGCTGGCAGAAGGCTACGAGCGGCTGACCGCCGATCTCAAGGCCCTGCGTGCGGAACGCCCGAA
GGTGGTCGACGCGATCGAAGAAGCGCGCGCGCATGGCGACCTGTCGGAAAATGCCGAATATCACGCGGCGAAGGAACGCC
AAGGCCAGATCGAAGCGCAGATCGCCGACATCGAAGACCGCGTAACCCGTGCCCAGATCATCGATCCCGCCACTTTATCG
GGCGACAAGATCGTGTTCGGTGCCACCGTGACCCTGCTCGATGAAGACGACAAGCCGGTCAAATACCAGATCGTCGGCCA
GACCGAAGCCGATGCCGCCAAGGGCCGCATCAGCTACAGCTCGCCCATCGGTCGCGCGCTGATCGGCAAGCAGGTCGGTG
ACGAGATCGAAGTGACTGTGCCCTCGGGTGATAAGTTCTACCTCGTCGACAAGGTCGAATTCGTCTGA

Upstream 100 bases:

>100_bases
GATATTGCCGAACCGCGCGACCAAAGGCGCGGCGAAGTCTCGAATTGACAATCGAACCGCGGGGCGGGGCACAAACGAAA
GAAATGAAGTGGGGACGGTA

Downstream 100 bases:

>100_bases
GGTTCGCTTCGGAAGCGGCTCAATCGAGCCGCTTTTCCATCGCATAATTGTGGATCGGCACGCCGCCGATTTTTAAGTCG
CGGCGCTGGATCACGGCGTA

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MEKVPMLAEGYERLTADLKALRAERPKVVDAIEEARAHGDLSENAEYHAAKERQGQIEAQIADIEDRVTRAQIIDPATLS
GDKIVFGATVTLLDEDDKPVKYQIVGQTEADAAKGRISYSSPIGRALIGKQVGDEIEVTVPSGDKFYLVDKVEFV

Sequences:

>Translated_155_residues
MEKVPMLAEGYERLTADLKALRAERPKVVDAIEEARAHGDLSENAEYHAAKERQGQIEAQIADIEDRVTRAQIIDPATLS
GDKIVFGATVTLLDEDDKPVKYQIVGQTEADAAKGRISYSSPIGRALIGKQVGDEIEVTVPSGDKFYLVDKVEFV
>Mature_155_residues
MEKVPMLAEGYERLTADLKALRAERPKVVDAIEEARAHGDLSENAEYHAAKERQGQIEAQIADIEDRVTRAQIIDPATLS
GDKIVFGATVTLLDEDDKPVKYQIVGQTEADAAKGRISYSSPIGRALIGKQVGDEIEVTVPSGDKFYLVDKVEFV

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=45.5696202531646, Blast_Score=133, Evalue=6e-33,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=35.4838709677419, Blast_Score=94, Evalue=5e-21,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 16984; Mature: 16984

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVPMLAEGYERLTADLKALRAERPKVVDAIEEARAHGDLSENAEYHAAKERQGQIEAQ
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEE
IADIEDRVTRAQIIDPATLSGDKIVFGATVTLLDEDDKPVKYQIVGQTEADAAKGRISYS
EECHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEECCCCCCHHCCEECCC
SPIGRALIGKQVGDEIEVTVPSGDKFYLVDKVEFV
CCCHHHHHHHHCCCEEEEEECCCCEEEEEEEEECC
>Mature Secondary Structure
MEKVPMLAEGYERLTADLKALRAERPKVVDAIEEARAHGDLSENAEYHAAKERQGQIEAQ
CCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEEEE
IADIEDRVTRAQIIDPATLSGDKIVFGATVTLLDEDDKPVKYQIVGQTEADAAKGRISYS
EECHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCCEEEEEECCCCCCHHCCEECCC
SPIGRALIGKQVGDEIEVTVPSGDKFYLVDKVEFV
CCCHHHHHHHHCCCEEEEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA