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The map label for this gene is dnaG [H]

Identifier: 85373961

GI number: 85373961

Start: 1172710

End: 1174587

Strand: Reverse

Name: dnaG [H]

Synonym: ELI_05670

Alternate gene names: 85373961

Gene position: 1174587-1172710 (Counterclockwise)

Preceding gene: 85373962

Following gene: 85373960

Centisome position: 38.48

GC content: 63.15

Gene sequence:

>1878_bases
ATGCTCTCACCCCAATGGCTTGACGAATTGCGTGCGCGGGTGACGCTTTCCAGCGTCATCATGCGCACGACCAAGCTGCA
AAGAGCGGGCCGCGAATGGAAGGCGTGCTGCCCATTCCATAACGAGAAAACGCCGAGTTTTACGGTCTCGGACGAAAAAG
GCTTTTACCACTGCTTCGGCTGCGGTGCGCATGGCGACGTGATCCGCTGGATGACCGACCAGCGCGGCCTGCAGTTCATG
GATGCGGTGAAGGAGCTGGCGCAGGAAGCGGGGCTGGAAGTCCCTGCCCCCGATCCGCGTGCGGCGCAGCAGGCGGAAAA
GCGCGCCAGCCTGATCGATGTGACCGAGGCTTCGCAGCGATGGTTCGTCGAACAATTGCGTGGTGCGGAGGGTGCCAGGG
CCCGCGACTATCTGGCCTCGCGCGGTTTCTCCAATGGCGTGATCCGCGAATTCGGCTTCGGCTACGCCCCGGACAGCAAG
CAGGCGATGGGCTCCGGTGTCGGTTTCGAGGACGCGATGCTCGTCGAAAGCGGTATGCGGATCGAGACAGAGGATGGCAG
CAAATACGACCGCTTTCGCGACCGGCTGATGCTGCCGATCCAGGATGCGCGGGGGCGCGTGATCGCGTTCGGCGGGCGCA
TTCTCGACAAGCGCGACGGCGTGGCAAAATATCTCAACTCGCCCGATACGCCGCTCTTCGACAAAGGGCGCACGCTTTAC
AACCTGCATCGGGCGGGCCCCGCCTCGCGCCAGACCGGCCGCATTGTCGTCGTCGAAGGCTATATGGACGTCATCGCCCT
CGCCAACGCCGGGATCGAGGATGCGGTCGCCCCGCTCGGAACCGCGCTGACGGAGCGGCAGATCGAAATGCTGTGGCGGA
TGGTCGAGCGGCCGATTCTGTGTTTCGATGGCGATGCCGCCGGCCAGCGCGCGGCGATGCGGGCGATCCAGCGCGCCCTT
CCCTTACTGCGCCCTGCCCATTCGCTTGGCATAGTGCGCCTGCCCGCGGGTCTCGATCCCGATGACCTTATCAATCAGCG
TGGGGCGCAAGCGATGGAAGATATCCTCGCGAAGCCCGGCAGCCTGCTCGATACGCTGTGGCAGTTCGAACGCGATGCCG
AGCCGCTCGACAGTCCCGAGGCGAAAGCGGGCCTCAAGGCGCGGCTGATGGCGCATGTCGATGCCATCGGCGATGCCGAT
ATCAAGGCACTCTACCGCCGCGAATTGCTCGACCGTTTTTCCGACTATGCCTTCCCGAGGCGCGAAAAAGCCCCTTGGAA
ACCTAATGGCTTTTCGAAGAGTCTCGCTTCCCGGCCCAGACCCGATCCCGCGGCGATCAGAGCGCTGCAAAAGACCATCC
AAGGCGGATCGCGCGACGCCTTGACGGGAGCGGTCGTTGCCGGGTTGATCCGCCATCCCGACCAGATCGACCGCCATGCG
GATGCGCTTGCCGAAGCGGCCGAGCGTGACGCGAATATCGCACGGGCGATAAATCCGCTGCTCGACATGGCGGAAATACT
TGAAGACGGTGGCGAATTGCCCATATCTGCCGATAGCGGCCGTCTTGCGCCCCCGGATAGAAGTCATTTCTCCTTCCTCC
AGGAAGGTACCGATCCCGAGGATGCGCGCGAAGACTTGGCCGAAGCCGTATCGTTGCTGGTCGAACGACCTGCGCTGGAA
ACTGCCCTTGCGGCAGCCACGGCGCGGTTCGATACCGACCCCGAAGGTGCTTTTGCCGAACAGCAGAGACTGTTGAAGCG
AAAGCTGGATTTCGAGGCGCGACTCGGCCAAATGGCGAGTTTACAGGCCGCCCGCGGGCAGTCTGATCTGGGGTATACGT
CACAGCCGGAACCGGCGGGCGAAGAAAAAACGGATTGA

Upstream 100 bases:

>100_bases
TGCGGACAGAGCGAGCAATGCGTGTCTGGCACCTGCCACCTCCACAGGCGAATAGGCCGCACTGCTTGAATGCGCGGGCG
TGAGAGCGCATTGAAGCACC

Downstream 100 bases:

>100_bases
AGCGAGACCTATGGCTTCCAAGGCGAAGAAGAAAACCCAGGCGGAAGATGCTCCCCTGATCGATCTCAACGAAGCTTCGA
TCAAGAAGATGATCACCAAG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MLSPQWLDELRARVTLSSVIMRTTKLQRAGREWKACCPFHNEKTPSFTVSDEKGFYHCFGCGAHGDVIRWMTDQRGLQFM
DAVKELAQEAGLEVPAPDPRAAQQAEKRASLIDVTEASQRWFVEQLRGAEGARARDYLASRGFSNGVIREFGFGYAPDSK
QAMGSGVGFEDAMLVESGMRIETEDGSKYDRFRDRLMLPIQDARGRVIAFGGRILDKRDGVAKYLNSPDTPLFDKGRTLY
NLHRAGPASRQTGRIVVVEGYMDVIALANAGIEDAVAPLGTALTERQIEMLWRMVERPILCFDGDAAGQRAAMRAIQRAL
PLLRPAHSLGIVRLPAGLDPDDLINQRGAQAMEDILAKPGSLLDTLWQFERDAEPLDSPEAKAGLKARLMAHVDAIGDAD
IKALYRRELLDRFSDYAFPRREKAPWKPNGFSKSLASRPRPDPAAIRALQKTIQGGSRDALTGAVVAGLIRHPDQIDRHA
DALAEAAERDANIARAINPLLDMAEILEDGGELPISADSGRLAPPDRSHFSFLQEGTDPEDAREDLAEAVSLLVERPALE
TALAAATARFDTDPEGAFAEQQRLLKRKLDFEARLGQMASLQAARGQSDLGYTSQPEPAGEEKTD

Sequences:

>Translated_625_residues
MLSPQWLDELRARVTLSSVIMRTTKLQRAGREWKACCPFHNEKTPSFTVSDEKGFYHCFGCGAHGDVIRWMTDQRGLQFM
DAVKELAQEAGLEVPAPDPRAAQQAEKRASLIDVTEASQRWFVEQLRGAEGARARDYLASRGFSNGVIREFGFGYAPDSK
QAMGSGVGFEDAMLVESGMRIETEDGSKYDRFRDRLMLPIQDARGRVIAFGGRILDKRDGVAKYLNSPDTPLFDKGRTLY
NLHRAGPASRQTGRIVVVEGYMDVIALANAGIEDAVAPLGTALTERQIEMLWRMVERPILCFDGDAAGQRAAMRAIQRAL
PLLRPAHSLGIVRLPAGLDPDDLINQRGAQAMEDILAKPGSLLDTLWQFERDAEPLDSPEAKAGLKARLMAHVDAIGDAD
IKALYRRELLDRFSDYAFPRREKAPWKPNGFSKSLASRPRPDPAAIRALQKTIQGGSRDALTGAVVAGLIRHPDQIDRHA
DALAEAAERDANIARAINPLLDMAEILEDGGELPISADSGRLAPPDRSHFSFLQEGTDPEDAREDLAEAVSLLVERPALE
TALAAATARFDTDPEGAFAEQQRLLKRKLDFEARLGQMASLQAARGQSDLGYTSQPEPAGEEKTD
>Mature_625_residues
MLSPQWLDELRARVTLSSVIMRTTKLQRAGREWKACCPFHNEKTPSFTVSDEKGFYHCFGCGAHGDVIRWMTDQRGLQFM
DAVKELAQEAGLEVPAPDPRAAQQAEKRASLIDVTEASQRWFVEQLRGAEGARARDYLASRGFSNGVIREFGFGYAPDSK
QAMGSGVGFEDAMLVESGMRIETEDGSKYDRFRDRLMLPIQDARGRVIAFGGRILDKRDGVAKYLNSPDTPLFDKGRTLY
NLHRAGPASRQTGRIVVVEGYMDVIALANAGIEDAVAPLGTALTERQIEMLWRMVERPILCFDGDAAGQRAAMRAIQRAL
PLLRPAHSLGIVRLPAGLDPDDLINQRGAQAMEDILAKPGSLLDTLWQFERDAEPLDSPEAKAGLKARLMAHVDAIGDAD
IKALYRRELLDRFSDYAFPRREKAPWKPNGFSKSLASRPRPDPAAIRALQKTIQGGSRDALTGAVVAGLIRHPDQIDRHA
DALAEAAERDANIARAINPLLDMAEILEDGGELPISADSGRLAPPDRSHFSFLQEGTDPEDAREDLAEAVSLLVERPALE
TALAAATARFDTDPEGAFAEQQRLLKRKLDFEARLGQMASLQAARGQSDLGYTSQPEPAGEEKTD

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=413, Percent_Identity=39.4673123486683, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68707; Mature: 68707

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSPQWLDELRARVTLSSVIMRTTKLQRAGREWKACCPFHNEKTPSFTVSDEKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEECCCCCEEEEEE
CGAHGDVIRWMTDQRGLQFMDAVKELAQEAGLEVPAPDPRAAQQAEKRASLIDVTEASQR
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
WFVEQLRGAEGARARDYLASRGFSNGVIREFGFGYAPDSKQAMGSGVGFEDAMLVESGMR
HHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCE
IETEDGSKYDRFRDRLMLPIQDARGRVIAFGGRILDKRDGVAKYLNSPDTPLFDKGRTLY
EECCCCCHHHHHHHHEECCEECCCCCEEEECCCHHCCCCHHHHHHCCCCCCCCCCCCHHH
NLHRAGPASRQTGRIVVVEGYMDVIALANAGIEDAVAPLGTALTERQIEMLWRMVERPIL
HHHHCCCCCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
CFDGDAAGQRAAMRAIQRALPLLRPAHSLGIVRLPAGLDPDDLINQRGAQAMEDILAKPG
EECCCCCHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SLLDTLWQFERDAEPLDSPEAKAGLKARLMAHVDAIGDADIKALYRRELLDRFSDYAFPR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
REKAPWKPNGFSKSLASRPRPDPAAIRALQKTIQGGSRDALTGAVVAGLIRHPDQIDRHA
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHH
DALAEAAERDANIARAINPLLDMAEILEDGGELPISADSGRLAPPDRSHFSFLQEGTDPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHH
DAREDLAEAVSLLVERPALETALAAATARFDTDPEGAFAEQQRLLKRKLDFEARLGQMAS
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LQAARGQSDLGYTSQPEPAGEEKTD
HHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLSPQWLDELRARVTLSSVIMRTTKLQRAGREWKACCPFHNEKTPSFTVSDEKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCEEECCCCCEEEEEE
CGAHGDVIRWMTDQRGLQFMDAVKELAQEAGLEVPAPDPRAAQQAEKRASLIDVTEASQR
CCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
WFVEQLRGAEGARARDYLASRGFSNGVIREFGFGYAPDSKQAMGSGVGFEDAMLVESGMR
HHHHHHCCCCCCHHHHHHHHCCCCCCCHHHCCCCCCCCCHHHHCCCCCCCHHHHHHCCCE
IETEDGSKYDRFRDRLMLPIQDARGRVIAFGGRILDKRDGVAKYLNSPDTPLFDKGRTLY
EECCCCCHHHHHHHHEECCEECCCCCEEEECCCHHCCCCHHHHHHCCCCCCCCCCCCHHH
NLHRAGPASRQTGRIVVVEGYMDVIALANAGIEDAVAPLGTALTERQIEMLWRMVERPIL
HHHHCCCCCCCCCCEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
CFDGDAAGQRAAMRAIQRALPLLRPAHSLGIVRLPAGLDPDDLINQRGAQAMEDILAKPG
EECCCCCHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
SLLDTLWQFERDAEPLDSPEAKAGLKARLMAHVDAIGDADIKALYRRELLDRFSDYAFPR
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCC
REKAPWKPNGFSKSLASRPRPDPAAIRALQKTIQGGSRDALTGAVVAGLIRHPDQIDRHA
CCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHH
DALAEAAERDANIARAINPLLDMAEILEDGGELPISADSGRLAPPDRSHFSFLQEGTDPE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHH
DAREDLAEAVSLLVERPALETALAAATARFDTDPEGAFAEQQRLLKRKLDFEARLGQMAS
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LQAARGQSDLGYTSQPEPAGEEKTD
HHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]