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The map label for this gene is glyA

Identifier: 85373957

GI number: 85373957

Start: 1167945

End: 1169249

Strand: Reverse

Name: glyA

Synonym: ELI_05650

Alternate gene names: 85373957

Gene position: 1169249-1167945 (Counterclockwise)

Preceding gene: 85373958

Following gene: 85373956

Centisome position: 38.31

GC content: 62.99

Gene sequence:

>1305_bases
ATGAGCACAGCCGAACAGACCCAGTCCGACATCATGCACCGTTTCTGGCACGACGATCTTGCTGCCGCCGATCCGGAAAT
CGCCGAAGCCGTGTCGAACGAGCTCAAGCGGCAGCAGGACAAGATCGAGCTGATCGCCAGCGAGAACATCGCCTCCAAGG
CCGTGCTCGAAGCGACCGGTAGCGTCTTCACCAACAAATATGCCGAGGGCTATCCGGGCAAGCGTTACTACGGCGGGTGC
GATTACGCCGATGTCGTCGAAACGCTGGCTATCGAGCGGGCGAAAGAGCTCTTCGGCTGCAATTTCGCCAATGTGCAGCC
CAATTCGGGCTCTCAGATGAACCAGGCAGTGTTCCTCGCGCTGCTGCAGCCGGGCGACACTTTCATGGGCCTCGACCTCA
ATTCGGGTGGCCACCTGACCCATGGATCACCGGTGAATATCAGCGGCAAGTGGTTCAATCCGGTCAGCTACGGTGTGCGC
AAGGACGACGAGCTCATCGACATGGACGAAGTTGCAGAGACTGCGCGCGAACACAAGCCCAAGCTGATCATCTGCGGCGG
CACCGCCTATTCGCGCCTGTGGGACTTCCCGCGCTTTCGCGAAATTGCCGATGAAGTCGACGCGACGCTGCTGTGCGACA
TGAGCCACATCTCCGGCCTCGTTGCAGGCGGCGCGCATCCCTCACCCTTCCCGCACGCGCATATCGTCACCAGCACCACG
CACAAGAGCCTGCGCGGTCCGCGCTCGGGCATCATCCTGTGGAATGACGAGGATTTGACCAAGCCGCTCAACATGGCGGT
CTTCCCCGGCCTGCAAGGCGGCCCGCTGATGCATGTCGTCGCGGCCAAGGCCGTCGCATTCCGCGAAGCGCTGCGGCCCG
ACTTCAGGACCTATGCCCACGCCGTGGTCGAGAACGCCCGTGCGCTCGCCGCCAGCCTCGAAGAGAACGGCCTGCGGATC
GTGTCGGGCGGGACCGACAATCATTCGATGCTGGTCGATCTCACGGCCAAGGATGTGACCGGCAAGGCCGCCGAAGCCGG
TCTCGACCGTGCATGGCTGACGTGCAACAAGAATGGCATCCCTTACGACACCCGCAGCCCTTTCGTGACCAGCGGCATTC
GCCTGGGCACGCCTGCCGGTACCACGCGTGGCTTCGGCCCGGCCGAGTTCCGCAAGGTCGGCGCGCTGATCGCGGAAGTG
GTAGACGGCCTGGCCAAGAACGGCCCCGAAGGCGATGCGCAGGTCGAAGAAAGTGTGCGCGGGCGGGTAAGTGAGCTATG
CTCGCAGTTCCCTGTTTACCCTTGA

Upstream 100 bases:

>100_bases
TGGCCAAGGCCTGCCTCACCGCCTTCCTCGAAACCGAATTCGGCGGCGGTCGTCACCAGCGCCGCGTCGACAAACTCTCC
AACCCGAACTGAGATCCGCC

Downstream 100 bases:

>100_bases
GGAACCGATGATGACCGGCACACCCCAACCGGAAGAGAATGACTGGGTCGAGAGCGCCCGCGAAGAGATCAAGGGCATGG
CGAGGCAAGGCATGAAGCAC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MSTAEQTQSDIMHRFWHDDLAAADPEIAEAVSNELKRQQDKIELIASENIASKAVLEATGSVFTNKYAEGYPGKRYYGGC
DYADVVETLAIERAKELFGCNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNISGKWFNPVSYGVR
KDDELIDMDEVAETAREHKPKLIICGGTAYSRLWDFPRFREIADEVDATLLCDMSHISGLVAGGAHPSPFPHAHIVTSTT
HKSLRGPRSGIILWNDEDLTKPLNMAVFPGLQGGPLMHVVAAKAVAFREALRPDFRTYAHAVVENARALAASLEENGLRI
VSGGTDNHSMLVDLTAKDVTGKAAEAGLDRAWLTCNKNGIPYDTRSPFVTSGIRLGTPAGTTRGFGPAEFRKVGALIAEV
VDGLAKNGPEGDAQVEESVRGRVSELCSQFPVYP

Sequences:

>Translated_434_residues
MSTAEQTQSDIMHRFWHDDLAAADPEIAEAVSNELKRQQDKIELIASENIASKAVLEATGSVFTNKYAEGYPGKRYYGGC
DYADVVETLAIERAKELFGCNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNISGKWFNPVSYGVR
KDDELIDMDEVAETAREHKPKLIICGGTAYSRLWDFPRFREIADEVDATLLCDMSHISGLVAGGAHPSPFPHAHIVTSTT
HKSLRGPRSGIILWNDEDLTKPLNMAVFPGLQGGPLMHVVAAKAVAFREALRPDFRTYAHAVVENARALAASLEENGLRI
VSGGTDNHSMLVDLTAKDVTGKAAEAGLDRAWLTCNKNGIPYDTRSPFVTSGIRLGTPAGTTRGFGPAEFRKVGALIAEV
VDGLAKNGPEGDAQVEESVRGRVSELCSQFPVYP
>Mature_433_residues
STAEQTQSDIMHRFWHDDLAAADPEIAEAVSNELKRQQDKIELIASENIASKAVLEATGSVFTNKYAEGYPGKRYYGGCD
YADVVETLAIERAKELFGCNFANVQPNSGSQMNQAVFLALLQPGDTFMGLDLNSGGHLTHGSPVNISGKWFNPVSYGVRK
DDELIDMDEVAETAREHKPKLIICGGTAYSRLWDFPRFREIADEVDATLLCDMSHISGLVAGGAHPSPFPHAHIVTSTTH
KSLRGPRSGIILWNDEDLTKPLNMAVFPGLQGGPLMHVVAAKAVAFREALRPDFRTYAHAVVENARALAASLEENGLRIV
SGGTDNHSMLVDLTAKDVTGKAAEAGLDRAWLTCNKNGIPYDTRSPFVTSGIRLGTPAGTTRGFGPAEFRKVGALIAEVV
DGLAKNGPEGDAQVEESVRGRVSELCSQFPVYP

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=471, Percent_Identity=45.6475583864119, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=471, Percent_Identity=45.6475583864119, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=471, Percent_Identity=45.6475583864119, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI19923315, Length=471, Percent_Identity=45.6475583864119, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=471, Percent_Identity=45.0106157112527, Blast_Score=349, Evalue=2e-96,
Organism=Homo sapiens, GI22547186, Length=413, Percent_Identity=46.7312348668281, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI22547189, Length=395, Percent_Identity=45.0632911392405, Blast_Score=299, Evalue=4e-81,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=55.3956834532374, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25144732, Length=422, Percent_Identity=45.7345971563981, Blast_Score=346, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=406, Percent_Identity=47.0443349753695, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=436, Percent_Identity=42.8899082568807, Blast_Score=342, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6323087, Length=419, Percent_Identity=41.527446300716, Blast_Score=325, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24640005, Length=461, Percent_Identity=43.1670281995662, Blast_Score=357, Evalue=1e-98,
Organism=Drosophila melanogaster, GI221329721, Length=461, Percent_Identity=43.1670281995662, Blast_Score=356, Evalue=2e-98,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_ERYLH (Q2NAR9)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_458019.1
- HSSP:   P34897
- ProteinModelPortal:   Q2NAR9
- SMR:   Q2NAR9
- STRING:   Q2NAR9
- GeneID:   3870681
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_05650
- NMPDR:   fig|314225.3.peg.1934
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   GRAYVTC
- PhylomeDB:   Q2NAR9
- ProtClustDB:   PRK00011
- BioCyc:   ELIT314225:ELI_05650-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46882; Mature: 46751

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAEQTQSDIMHRFWHDDLAAADPEIAEAVSNELKRQQDKIELIASENIASKAVLEATG
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SVFTNKYAEGYPGKRYYGGCDYADVVETLAIERAKELFGCNFANVQPNSGSQMNQAVFLA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEE
LLQPGDTFMGLDLNSGGHLTHGSPVNISGKWFNPVSYGVRKDDELIDMDEVAETAREHKP
EECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCC
KLIICGGTAYSRLWDFPRFREIADEVDATLLCDMSHISGLVAGGAHPSPFPHAHIVTSTT
CEEEECCCHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCEEEECCC
HKSLRGPRSGIILWNDEDLTKPLNMAVFPGLQGGPLMHVVAAKAVAFREALRPDFRTYAH
HHHHCCCCCCEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
AVVENARALAASLEENGLRIVSGGTDNHSMLVDLTAKDVTGKAAEAGLDRAWLTCNKNGI
HHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCHHHHHCCCCEEEEECCCCC
PYDTRSPFVTSGIRLGTPAGTTRGFGPAEFRKVGALIAEVVDGLAKNGPEGDAQVEESVR
CCCCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GRVSELCSQFPVYP
HHHHHHHHHCCCCC
>Mature Secondary Structure 
STAEQTQSDIMHRFWHDDLAAADPEIAEAVSNELKRQQDKIELIASENIASKAVLEATG
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SVFTNKYAEGYPGKRYYGGCDYADVVETLAIERAKELFGCNFANVQPNSGSQMNQAVFLA
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHEEEEE
LLQPGDTFMGLDLNSGGHLTHGSPVNISGKWFNPVSYGVRKDDELIDMDEVAETAREHKP
EECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCC
KLIICGGTAYSRLWDFPRFREIADEVDATLLCDMSHISGLVAGGAHPSPFPHAHIVTSTT
CEEEECCCHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHCCCCCCCCCCCCEEEECCC
HKSLRGPRSGIILWNDEDLTKPLNMAVFPGLQGGPLMHVVAAKAVAFREALRPDFRTYAH
HHHHCCCCCCEEEECCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHH
AVVENARALAASLEENGLRIVSGGTDNHSMLVDLTAKDVTGKAAEAGLDRAWLTCNKNGI
HHHHHHHHHHHHHHHCCEEEEECCCCCCEEEEEEECCCCCCHHHHHCCCCEEEEECCCCC
PYDTRSPFVTSGIRLGTPAGTTRGFGPAEFRKVGALIAEVVDGLAKNGPEGDAQVEESVR
CCCCCCCHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
GRVSELCSQFPVYP
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA