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The map label for this gene is trmJ [H]

Identifier: 85373954

GI number: 85373954

Start: 1166091

End: 1167245

Strand: Reverse

Name: trmJ [H]

Synonym: ELI_05635

Alternate gene names: 85373954

Gene position: 1167245-1166091 (Counterclockwise)

Preceding gene: 85373955

Following gene: 85373953

Centisome position: 38.24

GC content: 61.3

Gene sequence:

>1155_bases
ATGTTCTGGGCTTACATGATCCGTTGCGGCGACGGGACGTTCTACACGGGTCATACAGACGATCTTGAAATGAGATGGGC
TCAGCATCTCGATGGGTCTTTTGCGGGCTATACGGCATCGCGGCATCCGTTGGAGCTGGTATGGGCGGCTGACTTCGCTA
CGAGAGAAGAAGCGCTCGCGAGCGAATTGCGCATCAAGAAGTGGTCGCAAGCCAAGAAGGTTGGATTGATCGAAGGCGAC
TGGAACAAGGTTGCCCATTTTGCAAAACCTCCCTCTCGCAGGACGTCTCGACAAGCCATCTCGACAGGCTCGATGTCTGC
TCGACACGAACGGGTGAAAGGCACTGCCGTTCGTGTCGAGCGGGGTTCGAGCGAAGTCGAGAACCCCAGTCGAGACATCG
GCAAGCCGCAGAAACCCGCCATTGTCCTCGTTCGCCCGCAATTGGGCGAGAACATAGGCAATGCGGCACGGGCGATGCGC
AATTTCGGCCTCACCGAGCTGCGCCTGGTAGCGCCGCGCGACGGCTGGCCGAATCCTGACGCCGGCCCCTCTGCAGCCGG
AGCCGACGATGTGCTCGAACGCGCGCAGGTTTTTGCGACCACCGCGGAGGCCGTTGCGGATTGCGCGCATATCTACGCCA
CGACCGTGCGCAAGCGCGGGGTCACCAAACCCGTCGTGACGCCCGAGGAGGCGGCGCAGGCCATCCACAGCCAAGCGGGC
AAGTCGGCCGTCCTGTTCGGGCCCGAAAGGTCAGGACTGGAAACAGAGGACGTTGCTCTCGCTCGCGCTATACTGACGGT
CCCGATCGATCCCGAGTTCGGGTCGCTGAACCTTGCGCAGGCGGTCATCCTGTGCGCTTACGAATGGTCGAAAGGTGCCG
AACTCGAACAGCCGACGCAGGAGGACCTGCTGCCGCCTGCCCCGCAGGCCGAGCTAGACGATCTCATTGCACATCTGGAA
GCTATGCTGCAGCCGAAGGGCTATTTCCTCCCCGCAAACCGTACGGAAGCGACGCGGAGAACGCTCAGAACGGTGCTGAC
CAAGCCAGGCTGGAACCATTTGGAAATCCGAACCTTGCGCGGTGTTCTGTCTTCGCTGCAGCGTGGCGACAAAGTGCCGA
AAGGCCCCCTCGACAACGCCGAAAGTTCCGATTAG

Upstream 100 bases:

>100_bases
TCGCCTATATCCGCTTTGCCAGTGTCTATCGCGACTTCAGTGAGGCGCGGGACTTCGAGGAATTCGCCAGCACGGTGCAG
GAAGCGGCGAAGGACTAGAG

Downstream 100 bases:

>100_bases
CACGCGCGCTCCAAGTCCAATTCCCCAATCACAGCAACAGGATACGCTTCATGCTTTCCACCGTGGTCGCCCTCGCCCTG
CTCGCAAGCCCTGAAGGTGC

Product: rRNA methylase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MFWAYMIRCGDGTFYTGHTDDLEMRWAQHLDGSFAGYTASRHPLELVWAADFATREEALASELRIKKWSQAKKVGLIEGD
WNKVAHFAKPPSRRTSRQAISTGSMSARHERVKGTAVRVERGSSEVENPSRDIGKPQKPAIVLVRPQLGENIGNAARAMR
NFGLTELRLVAPRDGWPNPDAGPSAAGADDVLERAQVFATTAEAVADCAHIYATTVRKRGVTKPVVTPEEAAQAIHSQAG
KSAVLFGPERSGLETEDVALARAILTVPIDPEFGSLNLAQAVILCAYEWSKGAELEQPTQEDLLPPAPQAELDDLIAHLE
AMLQPKGYFLPANRTEATRRTLRTVLTKPGWNHLEIRTLRGVLSSLQRGDKVPKGPLDNAESSD

Sequences:

>Translated_384_residues
MFWAYMIRCGDGTFYTGHTDDLEMRWAQHLDGSFAGYTASRHPLELVWAADFATREEALASELRIKKWSQAKKVGLIEGD
WNKVAHFAKPPSRRTSRQAISTGSMSARHERVKGTAVRVERGSSEVENPSRDIGKPQKPAIVLVRPQLGENIGNAARAMR
NFGLTELRLVAPRDGWPNPDAGPSAAGADDVLERAQVFATTAEAVADCAHIYATTVRKRGVTKPVVTPEEAAQAIHSQAG
KSAVLFGPERSGLETEDVALARAILTVPIDPEFGSLNLAQAVILCAYEWSKGAELEQPTQEDLLPPAPQAELDDLIAHLE
AMLQPKGYFLPANRTEATRRTLRTVLTKPGWNHLEIRTLRGVLSSLQRGDKVPKGPLDNAESSD
>Mature_384_residues
MFWAYMIRCGDGTFYTGHTDDLEMRWAQHLDGSFAGYTASRHPLELVWAADFATREEALASELRIKKWSQAKKVGLIEGD
WNKVAHFAKPPSRRTSRQAISTGSMSARHERVKGTAVRVERGSSEVENPSRDIGKPQKPAIVLVRPQLGENIGNAARAMR
NFGLTELRLVAPRDGWPNPDAGPSAAGADDVLERAQVFATTAEAVADCAHIYATTVRKRGVTKPVVTPEEAAQAIHSQAG
KSAVLFGPERSGLETEDVALARAILTVPIDPEFGSLNLAQAVILCAYEWSKGAELEQPTQEDLLPPAPQAELDDLIAHLE
AMLQPKGYFLPANRTEATRRTLRTVLTKPGWNHLEIRTLRGVLSSLQRGDKVPKGPLDNAESSD

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=241, Percent_Identity=33.195020746888, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790865, Length=165, Percent_Identity=35.7575757575758, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 41981; Mature: 41981

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFWAYMIRCGDGTFYTGHTDDLEMRWAQHLDGSFAGYTASRHPLELVWAADFATREEALA
CCEEEEEEECCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
SELRIKKWSQAKKVGLIEGDWNKVAHFAKPPSRRTSRQAISTGSMSARHERVKGTAVRVE
HHHHHHHHHHHHHCCEEECCHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCEEEEEE
RGSSEVENPSRDIGKPQKPAIVLVRPQLGENIGNAARAMRNFGLTELRLVAPRDGWPNPD
CCCCCCCCCHHHCCCCCCCEEEEECCHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
AGPSAAGADDVLERAQVFATTAEAVADCAHIYATTVRKRGVTKPVVTPEEAAQAIHSQAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
KSAVLFGPERSGLETEDVALARAILTVPIDPEFGSLNLAQAVILCAYEWSKGAELEQPTQ
CEEEEECCCCCCCCHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCH
EDLLPPAPQAELDDLIAHLEAMLQPKGYFLPANRTEATRRTLRTVLTKPGWNHLEIRTLR
HHCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
GVLSSLQRGDKVPKGPLDNAESSD
HHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFWAYMIRCGDGTFYTGHTDDLEMRWAQHLDGSFAGYTASRHPLELVWAADFATREEALA
CCEEEEEEECCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHH
SELRIKKWSQAKKVGLIEGDWNKVAHFAKPPSRRTSRQAISTGSMSARHERVKGTAVRVE
HHHHHHHHHHHHHCCEEECCHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCEEEEEE
RGSSEVENPSRDIGKPQKPAIVLVRPQLGENIGNAARAMRNFGLTELRLVAPRDGWPNPD
CCCCCCCCCHHHCCCCCCCEEEEECCHHCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
AGPSAAGADDVLERAQVFATTAEAVADCAHIYATTVRKRGVTKPVVTPEEAAQAIHSQAG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
KSAVLFGPERSGLETEDVALARAILTVPIDPEFGSLNLAQAVILCAYEWSKGAELEQPTQ
CEEEEECCCCCCCCHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCH
EDLLPPAPQAELDDLIAHLEAMLQPKGYFLPANRTEATRRTLRTVLTKPGWNHLEIRTLR
HHCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHH
GVLSSLQRGDKVPKGPLDNAESSD
HHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]