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The map label for this gene is phoB [H]

Identifier: 85373907

GI number: 85373907

Start: 1122611

End: 1123300

Strand: Reverse

Name: phoB [H]

Synonym: ELI_05400

Alternate gene names: 85373907

Gene position: 1123300-1122611 (Counterclockwise)

Preceding gene: 85373908

Following gene: 85373902

Centisome position: 36.8

GC content: 64.78

Gene sequence:

>690_bases
TTGCCTAAAGCGAAACTCCTTCTGGTAGAGGACGACCCCGCCCTGGCGGAGCTCCTCGAATATCGGTTCACGAACGAAGG
CTATGGCGTGCGCACCACGTCGGACGGCGACGAAGCGCTTCTCATGGCGAGCGAGGACGTACCGGACCTGGTCATCCTCG
ACTGGATGATCGAAGGCACCAGCGGGATAGAGGTCTGCCGCCGGTTGCGGCGCGAAAAGGAAACCGCGCACGTCCCTATC
ATCATGTTGACCGCGCGAGAGGCGGAGGACGACCGCGTTCGCGGGCTCGATACCGGCGCCGACGATTACATGGTCAAGCC
GTTCAGCCCGCGCGAATTGCTGGCGCGCGTCGCCGCCGTGATGCGGCGCATCCGCCCTGCCCTTGCCGGCGAGACGATCC
AGGCCGGCGACATCGTGCTCGATCCGGTCGCGCACAAGGTGTCACGCCGCGGTGAGACGCTACAGCTCGGGCCTACCGAG
TATCGCCTGCTCAAGTTCTTCATGGAAAGTCCGGGGCGCGTCTTCAGCCGCGGGCAATTGCTCGATGGCGTGTGGGGCAC
GGGCAGCGATATCGAACTGCGCACGGTCGATGTGCACATTCGCCGTCTGCGCAAGGCAATCGACGTGCCAGGGGCGGACG
ATCCGATTCGCACCGTCCGCTCGGCCGGATACGCGCTGGAGACGAATTAG

Upstream 100 bases:

>100_bases
TATCCGCCCGATGATGACTAAAAACTGTCATCGTTGAAACAACACTGACCCAAGATTGCAATATCGCCCCTCGCAAGGCT
ATCGAAGCGGGGTGGTTGAG

Downstream 100 bases:

>100_bases
CGGCAGACGCCGCCGAAGCCGCGCGCCTGCTGGTCGAAATGGAAGTGATCGCGGTGCGCGGCATTGTAATCGGGTGACAG
CACCGTCCCGAACACCTCGC

Product: phosphate regulon response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MPKAKLLLVEDDPALAELLEYRFTNEGYGVRTTSDGDEALLMASEDVPDLVILDWMIEGTSGIEVCRRLRREKETAHVPI
IMLTAREAEDDRVRGLDTGADDYMVKPFSPRELLARVAAVMRRIRPALAGETIQAGDIVLDPVAHKVSRRGETLQLGPTE
YRLLKFFMESPGRVFSRGQLLDGVWGTGSDIELRTVDVHIRRLRKAIDVPGADDPIRTVRSAGYALETN

Sequences:

>Translated_229_residues
MPKAKLLLVEDDPALAELLEYRFTNEGYGVRTTSDGDEALLMASEDVPDLVILDWMIEGTSGIEVCRRLRREKETAHVPI
IMLTAREAEDDRVRGLDTGADDYMVKPFSPRELLARVAAVMRRIRPALAGETIQAGDIVLDPVAHKVSRRGETLQLGPTE
YRLLKFFMESPGRVFSRGQLLDGVWGTGSDIELRTVDVHIRRLRKAIDVPGADDPIRTVRSAGYALETN
>Mature_228_residues
PKAKLLLVEDDPALAELLEYRFTNEGYGVRTTSDGDEALLMASEDVPDLVILDWMIEGTSGIEVCRRLRREKETAHVPII
MLTAREAEDDRVRGLDTGADDYMVKPFSPRELLARVAAVMRRIRPALAGETIQAGDIVLDPVAHKVSRRGETLQLGPTEY
RLLKFFMESPGRVFSRGQLLDGVWGTGSDIELRTVDVHIRRLRKAIDVPGADDPIRTVRSAGYALETN

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=224, Percent_Identity=50, Blast_Score=228, Evalue=2e-61,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=39.2070484581498, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI87082012, Length=222, Percent_Identity=40.5405405405405, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=40.7894736842105, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786784, Length=224, Percent_Identity=37.5, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1789809, Length=229, Percent_Identity=36.2445414847162, Blast_Score=132, Evalue=1e-32,
Organism=Escherichia coli, GI1787375, Length=223, Percent_Identity=33.1838565022422, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=34.6666666666667, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=33.7606837606838, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI2367329, Length=230, Percent_Identity=37.3913043478261, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786911, Length=214, Percent_Identity=33.6448598130841, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1790552, Length=225, Percent_Identity=35.1111111111111, Blast_Score=113, Evalue=9e-27,
Organism=Escherichia coli, GI1787229, Length=233, Percent_Identity=34.3347639484979, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI145693140, Length=232, Percent_Identity=30.1724137931034, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25542; Mature: 25410

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKAKLLLVEDDPALAELLEYRFTNEGYGVRTTSDGDEALLMASEDVPDLVILDWMIEGT
CCCCEEEEEECCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEEEECCC
SGIEVCRRLRREKETAHVPIIMLTAREAEDDRVRGLDTGADDYMVKPFSPRELLARVAAV
CHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCCCCCCCCCCEECCCCHHHHHHHHHHH
MRRIRPALAGETIQAGDIVLDPVAHKVSRRGETLQLGPTEYRLLKFFMESPGRVFSRGQL
HHHHHHHHCCCCCCCCCEEECHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCHHCCCE
LDGVWGTGSDIELRTVDVHIRRLRKAIDVPGADDPIRTVRSAGYALETN
ECCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEECC
>Mature Secondary Structure 
PKAKLLLVEDDPALAELLEYRFTNEGYGVRTTSDGDEALLMASEDVPDLVILDWMIEGT
CCCEEEEEECCHHHHHHHHHHCCCCCCCEEECCCCCCEEEEECCCCCCEEEEEEEECCC
SGIEVCRRLRREKETAHVPIIMLTAREAEDDRVRGLDTGADDYMVKPFSPRELLARVAAV
CHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHCCCCCCCCCCEECCCCHHHHHHHHHHH
MRRIRPALAGETIQAGDIVLDPVAHKVSRRGETLQLGPTEYRLLKFFMESPGRVFSRGQL
HHHHHHHHCCCCCCCCCEEECHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCHHCCCE
LDGVWGTGSDIELRTVDVHIRRLRKAIDVPGADDPIRTVRSAGYALETN
ECCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]