Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 85373848

GI number: 85373848

Start: 1054682

End: 1055470

Strand: Reverse

Name: ppnK

Synonym: ELI_05105

Alternate gene names: 85373848

Gene position: 1055470-1054682 (Counterclockwise)

Preceding gene: 85373850

Following gene: 85373844

Centisome position: 34.58

GC content: 61.72

Gene sequence:

>789_bases
TTGGGCCAGAATAATTCCTTCAAAACGCTCGCCCTGCTTGCTGCGGACACCGAGCGCGGCCAGGAAGCCGCCGAGAATCT
TTCCGCGCAATTCGATTTCACCCCGATCGAAGATGCCGATGCAGTGGTCGTCCTCGGCGGCGACGGCTTCATGCTTCAGA
CCCTGCACCAGATGCTCGACGAGGAGGCCGTCAAACCTGCCTATGGCATCAACATGGGCACCGTCGGCTTCCTGATGAAC
AAGCCGCGCAAGCGGCCCGACCTGCTCGGCGCAGTCAATCGCGCGTCGATCCGCAGGGTCGCCCCGCTGCGCACCGAAGT
GAAATTGCAAAATGGATCGGTTGCCACCGCCTGCGCGATCAACGAGGTGTCCTTGCTGCGCGAGACCCGTCAGACGGCGA
AGATAGAGGTCTCGGTCGGCGGCAAGATGCGCATCGGCGAGCTTGTCTGCGATGGCGTACTCGTCTCCACGCCGGTCGGA
TCGACCGCCTACAACCTGTCCGCCAATGGCCCGATCCTGCCCCTCGATTCGCAATTGCTGGCGCTGACGCCGATCTCTGC
CTTCCGTCCCCGGCGCTGGCGCGGTGCCATTCTGCCGGAATACAGCGACATCAAACTGCGCGTACTGGAACCTCACAAGC
GGCCCGTCGCGGTAGTCGCCGATCAACGCGAATACCGCGATATTGCCGAAGTCTCCGTCGCGATTGAGCGCTCGTCCGAA
TTGACCTTACTGTTTGACCCGGGCCATTCGCTGGACGAGCGGATAGTGGCCGAACAATTTGTCACTTGA

Upstream 100 bases:

>100_bases
AGCCTCTGCCTGGGCAACCATCCCGCTCAAAAGCCGGTGCGTTGCCAAATGGCACGGTCCTCCATAGGAAGCCTGCGGAC
GGCAATAGTGGAGAGCGGGT

Downstream 100 bases:

>100_bases
GGCACTTGCAAAACGATTCCGCCGCCCATATACGCGCGGCTCGCTCGCAAGACGGGCTGCTCCCCGATAGCTCAGCGGTA
GAGTAGGTGACTGTTAATCA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MGQNNSFKTLALLAADTERGQEAAENLSAQFDFTPIEDADAVVVLGGDGFMLQTLHQMLDEEAVKPAYGINMGTVGFLMN
KPRKRPDLLGAVNRASIRRVAPLRTEVKLQNGSVATACAINEVSLLRETRQTAKIEVSVGGKMRIGELVCDGVLVSTPVG
STAYNLSANGPILPLDSQLLALTPISAFRPRRWRGAILPEYSDIKLRVLEPHKRPVAVVADQREYRDIAEVSVAIERSSE
LTLLFDPGHSLDERIVAEQFVT

Sequences:

>Translated_262_residues
MGQNNSFKTLALLAADTERGQEAAENLSAQFDFTPIEDADAVVVLGGDGFMLQTLHQMLDEEAVKPAYGINMGTVGFLMN
KPRKRPDLLGAVNRASIRRVAPLRTEVKLQNGSVATACAINEVSLLRETRQTAKIEVSVGGKMRIGELVCDGVLVSTPVG
STAYNLSANGPILPLDSQLLALTPISAFRPRRWRGAILPEYSDIKLRVLEPHKRPVAVVADQREYRDIAEVSVAIERSSE
LTLLFDPGHSLDERIVAEQFVT
>Mature_261_residues
GQNNSFKTLALLAADTERGQEAAENLSAQFDFTPIEDADAVVVLGGDGFMLQTLHQMLDEEAVKPAYGINMGTVGFLMNK
PRKRPDLLGAVNRASIRRVAPLRTEVKLQNGSVATACAINEVSLLRETRQTAKIEVSVGGKMRIGELVCDGVLVSTPVGS
TAYNLSANGPILPLDSQLLALTPISAFRPRRWRGAILPEYSDIKLRVLEPHKRPVAVVADQREYRDIAEVSVAIERSSEL
TLLFDPGHSLDERIVAEQFVT

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=186, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6325068, Length=182, Percent_Identity=31.3186813186813, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 28563; Mature: 28432

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQNNSFKTLALLAADTERGQEAAENLSAQFDFTPIEDADAVVVLGGDGFMLQTLHQMLD
CCCCCCCEEEEEEEECCHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCHHHHHHHHHHH
EEAVKPAYGINMGTVGFLMNKPRKRPDLLGAVNRASIRRVAPLRTEVKLQNGSVATACAI
HHHCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCHHHHHHHCCCCEEEEECCCCEEEEEEH
NEVSLLRETRQTAKIEVSVGGKMRIGELVCDGVLVSTPVGSTAYNLSANGPILPLDSQLL
HHHHHHHHHHHHEEEEEEECCCEEEHHHHCCCEEEECCCCCCEEEECCCCCEEECCCCEE
ALTPISAFRPRRWRGAILPEYSDIKLRVLEPHKRPVAVVADQREYRDIAEVSVAIERSSE
EECCHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHHEEEEECCCC
LTLLFDPGHSLDERIVAEQFVT
EEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
GQNNSFKTLALLAADTERGQEAAENLSAQFDFTPIEDADAVVVLGGDGFMLQTLHQMLD
CCCCCCEEEEEEEECCHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCHHHHHHHHHHH
EEAVKPAYGINMGTVGFLMNKPRKRPDLLGAVNRASIRRVAPLRTEVKLQNGSVATACAI
HHHCCCCCCCCCCHHHHHHCCCCCCCHHHHHCCHHHHHHHCCCCEEEEECCCCEEEEEEH
NEVSLLRETRQTAKIEVSVGGKMRIGELVCDGVLVSTPVGSTAYNLSANGPILPLDSQLL
HHHHHHHHHHHHEEEEEEECCCEEEHHHHCCCEEEECCCCCCEEEECCCCCEEECCCCEE
ALTPISAFRPRRWRGAILPEYSDIKLRVLEPHKRPVAVVADQREYRDIAEVSVAIERSSE
EECCHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEECCHHHHHHHHHEEEEECCCC
LTLLFDPGHSLDERIVAEQFVT
EEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA