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The map label for this gene is bhbA [H]

Identifier: 85373809

GI number: 85373809

Start: 1012541

End: 1014694

Strand: Direct

Name: bhbA [H]

Synonym: ELI_04910

Alternate gene names: 85373809

Gene position: 1012541-1014694 (Clockwise)

Preceding gene: 85373808

Following gene: 85373810

Centisome position: 33.17

GC content: 63.18

Gene sequence:

>2154_bases
ATGACTGACAAACCGACCGTTTCCGATTGGGAAGCCCTTGCGGACAAGGAGAGCAAGGGTCGCGACCTCACCTGGGACAC
GCCCGAGGGCTTCACGATCAAGCCGCTCTATACGGCGGAGGACACCGCTGACCTTGATCCCGGCCTGCCGGGATTCGCGC
CTTTCACGCGCGGGGTGAAGGCTTCGATGTATGCCGGGCGGCCGTGGACGATCCGCCAATATGCGGGTTTCTCGACTGCC
GAAGAATCCAACGCCTTTTATCGCCGCAATCTCGCGGCGGGCCAGAAGGGCCTCTCGGTAGCCTTCGATCTCGCCACGCA
CCGCGGCTATGACAGCGACCATCCGCGCGTCGTCGGCGATGTCGGCAAGGCGGGCGTTGCGATTGATACCGTCGCGGACA
TGGAAATCCTGTTCGACCAGATCCCGCTCGATTCGATGTCGGTCAGCATGACGATGAACGGTGCGGTGATCCCCGTGCTG
GCGTTCTACATCGTCGCAGGCGAGCGGCAGGGGGTGAGCGCCGAGCAGCTTTCCGGGACCATCCAGAACGACATCCTCAA
GGAGTTCATGGTCCGCAACACCTATATCTATCCGCCCGAACCGAGCATGCGGATCGTTAGCGACATCATCGCCTACACTT
CCGCCAACATGCCGAAATTCAACAGCATTTCGATTTCGGGCTATCACATGCACGAGGCCGGGGCGACGGCGGTGCAGGAA
CTCGCCTTTACCATCGCCGACGGCAAGGAATATGCCGAGCGGGCGATGGCGACCGGGCTCGATATCGACGCCTTTGCGGG
CCGACTGAGCTTCTTCTTCGGCATCGGCATGAACTTCTTCATGGAGATCGCCAAGCTGCGCGCGGCGCGCACGCTGTGGT
GGAAGGTGATGGACGGGCTGGGCGCCAAGTCCGAACGCTCCAAGATGCTACGAACCCATTGCCAGACATCGGGCGTCAGC
TTGCAGGAGCAGGACCCCTACAACAACGTGATCCGCACCACGATCGAAGCAATGGCCGCGACGCTGGGCGGCACGCAGTC
GCTGCACACCAACGCGCTCGATGAAGCCATCGCGTTGCCGACCGATTTCTCCGCCCGCATTGCTCGCAATACGCAATTGG
TGCTGCAAGAAGAAAGCGGTATCACCAATGTCGTCGATCCGCTCGGCGGCTCTTACTACATCGAAGCGCTGACTGCGAAG
CTGGTCGAAGAGGCCGAGGTTATGCTGGAACAGATCGAGGCGGAAGGCGGTATGACCGCCTATGTCGCGACCGGCAAACC
CAAGGCCGCCATCGAACGCGCGGCGGCGGAAAAGCAGACTAGCGTCGACAAGGGCGAAACGGTGATCGTTGGCGTCAACA
AGTACCGCCGCGATGTCGAGGACGAGATCGACACGCTCGATATCGACAACCACAAGGTCCGGCAGGGCCAGATCGCGCGC
CTCAAGCGCGTCCGCGACGAGCGCGACGAGGCGGCATGCCAGGCTGCGCTTGAAGAGCTCACCAAGGGAGGGGCCGGTGG
CGGTAACGTGTTGGAGCTGGCTGTCAAAGCCGCGCGCCATGACGCGACCTTGGGCGAAATCTCCAGCGCGATGGAAGAGG
TCTTCGGCCGCCACGATGCCACACCCAAGCCCGTCAGCGGCGTGTACAAGAGCGCCTATGAATTCGACCACCGCTGGCAG
CAGGTCACCGACGGTGTCGAAGCGGTCGAACGCCGCTTGGGCCGCAAGCCGAGGATCATGGTCGCCAAGATGGGCCAGGA
CGGCCACGACCGCGGCGCAAATGTGATCGCCAGCGCCTTCGCCGACATGGGCTTCGAAGTCGTCTCCGGCCCGCTGTTCC
AGACGCCGAAGGAAGCCATGGCGATGGCGCTGGAAAACGGCGTCGATGCCATCGGTGCCAGCTCGCTCGCAGCGGGGCAC
AAGACGCTTATCCCGGAACTCATCGACCTGCTCAGGGAAGCGGGCCGCAGCGATATCAAGGTCGTCGCGGGCGGCGTGAT
ACCGCCGCAGGATTACGACTTCCTGCGCGAGGCCGGGGTGCAGGGTATTTACGGCCCGGGAAGCAACGTGGTCGAATGCG
GGGCCGACATGCTGCGCCTGCTCGGCCACAACATGCCGCCTGCCGGGGATGAGCTGGACGAGGCGGCGGAGTGA

Upstream 100 bases:

>100_bases
TTCGGCTGGCTCCTAAGCAGCCGCTCAGGATAAACTACGTCGAGATGCAATTGCCAAAGGTGTCTCGACTTGGCTCGACA
CGAACGGGTAAGGGACCCGC

Downstream 100 bases:

>100_bases
GTCCTGTTCAAATCTCGCCGAGCGGTCATGCGGATGCCGCCATCTTTGCAAGTTGGATGCGGGAAACGGGTGGATTGATT
GCCAAGCATTGGGCTTGTGA

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 717; Mature: 716

Protein sequence:

>717_residues
MTDKPTVSDWEALADKESKGRDLTWDTPEGFTIKPLYTAEDTADLDPGLPGFAPFTRGVKASMYAGRPWTIRQYAGFSTA
EESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDTVADMEILFDQIPLDSMSVSMTMNGAVIPVL
AFYIVAGERQGVSAEQLSGTIQNDILKEFMVRNTYIYPPEPSMRIVSDIIAYTSANMPKFNSISISGYHMHEAGATAVQE
LAFTIADGKEYAERAMATGLDIDAFAGRLSFFFGIGMNFFMEIAKLRAARTLWWKVMDGLGAKSERSKMLRTHCQTSGVS
LQEQDPYNNVIRTTIEAMAATLGGTQSLHTNALDEAIALPTDFSARIARNTQLVLQEESGITNVVDPLGGSYYIEALTAK
LVEEAEVMLEQIEAEGGMTAYVATGKPKAAIERAAAEKQTSVDKGETVIVGVNKYRRDVEDEIDTLDIDNHKVRQGQIAR
LKRVRDERDEAACQAALEELTKGGAGGGNVLELAVKAARHDATLGEISSAMEEVFGRHDATPKPVSGVYKSAYEFDHRWQ
QVTDGVEAVERRLGRKPRIMVAKMGQDGHDRGANVIASAFADMGFEVVSGPLFQTPKEAMAMALENGVDAIGASSLAAGH
KTLIPELIDLLREAGRSDIKVVAGGVIPPQDYDFLREAGVQGIYGPGSNVVECGADMLRLLGHNMPPAGDELDEAAE

Sequences:

>Translated_717_residues
MTDKPTVSDWEALADKESKGRDLTWDTPEGFTIKPLYTAEDTADLDPGLPGFAPFTRGVKASMYAGRPWTIRQYAGFSTA
EESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDTVADMEILFDQIPLDSMSVSMTMNGAVIPVL
AFYIVAGERQGVSAEQLSGTIQNDILKEFMVRNTYIYPPEPSMRIVSDIIAYTSANMPKFNSISISGYHMHEAGATAVQE
LAFTIADGKEYAERAMATGLDIDAFAGRLSFFFGIGMNFFMEIAKLRAARTLWWKVMDGLGAKSERSKMLRTHCQTSGVS
LQEQDPYNNVIRTTIEAMAATLGGTQSLHTNALDEAIALPTDFSARIARNTQLVLQEESGITNVVDPLGGSYYIEALTAK
LVEEAEVMLEQIEAEGGMTAYVATGKPKAAIERAAAEKQTSVDKGETVIVGVNKYRRDVEDEIDTLDIDNHKVRQGQIAR
LKRVRDERDEAACQAALEELTKGGAGGGNVLELAVKAARHDATLGEISSAMEEVFGRHDATPKPVSGVYKSAYEFDHRWQ
QVTDGVEAVERRLGRKPRIMVAKMGQDGHDRGANVIASAFADMGFEVVSGPLFQTPKEAMAMALENGVDAIGASSLAAGH
KTLIPELIDLLREAGRSDIKVVAGGVIPPQDYDFLREAGVQGIYGPGSNVVECGADMLRLLGHNMPPAGDELDEAAE
>Mature_716_residues
TDKPTVSDWEALADKESKGRDLTWDTPEGFTIKPLYTAEDTADLDPGLPGFAPFTRGVKASMYAGRPWTIRQYAGFSTAE
ESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGDVGKAGVAIDTVADMEILFDQIPLDSMSVSMTMNGAVIPVLA
FYIVAGERQGVSAEQLSGTIQNDILKEFMVRNTYIYPPEPSMRIVSDIIAYTSANMPKFNSISISGYHMHEAGATAVQEL
AFTIADGKEYAERAMATGLDIDAFAGRLSFFFGIGMNFFMEIAKLRAARTLWWKVMDGLGAKSERSKMLRTHCQTSGVSL
QEQDPYNNVIRTTIEAMAATLGGTQSLHTNALDEAIALPTDFSARIARNTQLVLQEESGITNVVDPLGGSYYIEALTAKL
VEEAEVMLEQIEAEGGMTAYVATGKPKAAIERAAAEKQTSVDKGETVIVGVNKYRRDVEDEIDTLDIDNHKVRQGQIARL
KRVRDERDEAACQAALEELTKGGAGGGNVLELAVKAARHDATLGEISSAMEEVFGRHDATPKPVSGVYKSAYEFDHRWQQ
VTDGVEAVERRLGRKPRIMVAKMGQDGHDRGANVIASAFADMGFEVVSGPLFQTPKEAMAMALENGVDAIGASSLAAGHK
TLIPELIDLLREAGRSDIKVVAGGVIPPQDYDFLREAGVQGIYGPGSNVVECGADMLRLLGHNMPPAGDELDEAAE

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=703, Percent_Identity=59.8862019914651, Blast_Score=854, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=710, Percent_Identity=61.9718309859155, Blast_Score=875, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=715, Percent_Identity=62.2377622377622, Blast_Score=880, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 77570; Mature: 77439

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKPTVSDWEALADKESKGRDLTWDTPEGFTIKPLYTAEDTADLDPGLPGFAPFTRGVK
CCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCC
ASMYAGRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGD
HHEECCCCEEEHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCEEEEC
VGKAGVAIDTVADMEILFDQIPLDSMSVSMTMNGAVIPVLAFYIVAGERQGVSAEQLSGT
CCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHH
IQNDILKEFMVRNTYIYPPEPSMRIVSDIIAYTSANMPKFNSISISGYHMHEAGATAVQE
HHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHH
LAFTIADGKEYAERAMATGLDIDAFAGRLSFFFGIGMNFFMEIAKLRAARTLWWKVMDGL
HHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAKSERSKMLRTHCQTSGVSLQEQDPYNNVIRTTIEAMAATLGGTQSLHTNALDEAIALP
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TDFSARIARNTQLVLQEESGITNVVDPLGGSYYIEALTAKLVEEAEVMLEQIEAEGGMTA
CCCHHHHHCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
YVATGKPKAAIERAAAEKQTSVDKGETVIVGVNKYRRDVEDEIDTLDIDNHKVRQGQIAR
EEECCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCEECCCCCCCCCHHHHH
LKRVRDERDEAACQAALEELTKGGAGGGNVLELAVKAARHDATLGEISSAMEEVFGRHDA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
TPKPVSGVYKSAYEFDHRWQQVTDGVEAVERRLGRKPRIMVAKMGQDGHDRGANVIASAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
ADMGFEVVSGPLFQTPKEAMAMALENGVDAIGASSLAAGHKTLIPELIDLLREAGRSDIK
HHCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEE
VVAGGVIPPQDYDFLREAGVQGIYGPGSNVVECGADMLRLLGHNMPPAGDELDEAAE
EEECCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCC
>Mature Secondary Structure 
TDKPTVSDWEALADKESKGRDLTWDTPEGFTIKPLYTAEDTADLDPGLPGFAPFTRGVK
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCC
ASMYAGRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVVGD
HHEECCCCEEEHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEEHHHHCCCCCCCCEEEEC
VGKAGVAIDTVADMEILFDQIPLDSMSVSMTMNGAVIPVLAFYIVAGERQGVSAEQLSGT
CCCCCEEEHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCHHHHHHH
IQNDILKEFMVRNTYIYPPEPSMRIVSDIIAYTSANMPKFNSISISGYHMHEAGATAVQE
HHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEEEHHHHHHHHHH
LAFTIADGKEYAERAMATGLDIDAFAGRLSFFFGIGMNFFMEIAKLRAARTLWWKVMDGL
HHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GAKSERSKMLRTHCQTSGVSLQEQDPYNNVIRTTIEAMAATLGGTQSLHTNALDEAIALP
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
TDFSARIARNTQLVLQEESGITNVVDPLGGSYYIEALTAKLVEEAEVMLEQIEAEGGMTA
CCCHHHHHCCCEEEEECCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
YVATGKPKAAIERAAAEKQTSVDKGETVIVGVNKYRRDVEDEIDTLDIDNHKVRQGQIAR
EEECCCCHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHHHHHHCEECCCCCCCCCHHHHH
LKRVRDERDEAACQAALEELTKGGAGGGNVLELAVKAARHDATLGEISSAMEEVFGRHDA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCC
TPKPVSGVYKSAYEFDHRWQQVTDGVEAVERRLGRKPRIMVAKMGQDGHDRGANVIASAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHH
ADMGFEVVSGPLFQTPKEAMAMALENGVDAIGASSLAAGHKTLIPELIDLLREAGRSDIK
HHCCHHHHCCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCEE
VVAGGVIPPQDYDFLREAGVQGIYGPGSNVVECGADMLRLLGHNMPPAGDELDEAAE
EEECCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]