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The map label for this gene is otsA [H]

Identifier: 85373765

GI number: 85373765

Start: 970229

End: 971614

Strand: Direct

Name: otsA [H]

Synonym: ELI_04690

Alternate gene names: 85373765

Gene position: 970229-971614 (Clockwise)

Preceding gene: 85373764

Following gene: 85373766

Centisome position: 31.79

GC content: 63.06

Gene sequence:

>1386_bases
GTGAGCCGCCTGGTCGTCATTTCCAATCGCGTCGCCGTGCCCAAGGCGCGCGGCGCGGCGGGGGCGCAGGGCGGGCTGGC
CGGCGCGCTCAACTCTGCGCTCAAGAAATTCGGCGGCATCTGGTTCGGCTGGTCGGGTCAGGAGAGCGAGGACCGCACCG
GCAATATCAACCTGCAGCGCAACGACGGCGTGACGACCGCGACCATCGATCTCTCGACCCGCGATATCGACGAATATTAC
AACGGCTACGCCAATTCGACGCTGTGGCCGCTGTTTCACTATCGCCTTGATCTCACTGAATATGAACGCGAAACCGGCAA
GGGCTACGAACGCGTCAACGAGCGGTTCGCGGAATGCGTAACCCCGCTGATCGAGGAAGACGACCTCGTCTGGGTGCACG
ACTACCACCTTCTGCCGCTGGGCGAACGGCTGCGCTCGCGCGGCCTCAAGAACCGCATCGGCTTCTTCCTCCACATCCCC
TGGCCACCGACGCGGCTGTTCGTCTCGCTGCCGTTCCATGACCGCTTGGTGAAGACCATGCTGGATTACGATGTGATCGG
TTTCCAGACGCAGGAATGGCTGGAAAGCTTCCTGCATTATTGCTCCAAGGAGCTGGGCGCGGAGGTGGACGAGGAGACGG
GCCGGATTTCCTACGAGGGCCGCACCGTGCTCGCCAAGGCCTATCCGATCGGTATCGACTGGGATCATTTCCAGGCGCAG
GGCGAAACCGGTGAAGCGCGGCAGGCGCAGCAGCGCCTGATGTCATCCGTACGCCACCGCACCGCGATGATCGGGGTCGA
CCGGCTCGATTATTCCAAGGGACTGCCGGAGCGTATCGACGGCATCGGGCGCTTCTTCGACCAGCATCCGGATCGTGTAC
GTGACCTCGTCTTTATCCAGATTGCGCCGCCGAGCCGCGAGGATGTCGAGAGCTACCAGCAGATCCGCACCGTGCTCGAG
CAGAAGAGCGGCCAGATCAATGGCGCGCGCTCCGAAGTCGATGTCGTACCGATCCGTTATGTCAACCGCGGCTACAGCCA
TGCCGAGCTGTATGGCTTCTTCCGCGCCAGCAAGATCGGGCTTGTCACGCCCCTTCGTGACGGAATGAACCTGGTGGCGA
AAGAATATCTCGCCGCGCAGGACCCGGAAGACCCCGGCGTGCTGATCCTCTCGCGCTTTGCCGGCGCGGCGGCGCAGTTG
CCCGATGCGGTACTGGTCAATCCGCATAGCCCCGACGATCTCAGCCATGCGATCAAGAAGGCGCTCGACATGCCGCTCGA
CGAGCGCAAGCGGCGCTACGAAGGCACGATCGGCACCGTGCGCGACGACAATGTGCAGGACTGGACCGAGGCGTTTACCA
ACGATCTCGCGTCGGTGCCGATCTAG

Upstream 100 bases:

>100_bases
ACGGGCGAACTGTGGGGGAACTTCCCGCAGACCTACTCGCTGGTAGGTATAATCAACTGTGCGGGGCTGTTGTCCAAACC
ATGGAGCGAGGTGCGATGAA

Downstream 100 bases:

>100_bases
GGTTGAGCGACCGGATACGCCGTGCCACTGGACTTTGCATGACAGACAACCGCGTGCTGCCGCTGCAACAGGTCCATAAT
TTCCGCGATTACGGGGGCTA

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSRLVVISNRVAVPKARGAAGAQGGLAGALNSALKKFGGIWFGWSGQESEDRTGNINLQRNDGVTTATIDLSTRDIDEYY
NGYANSTLWPLFHYRLDLTEYERETGKGYERVNERFAECVTPLIEEDDLVWVHDYHLLPLGERLRSRGLKNRIGFFLHIP
WPPTRLFVSLPFHDRLVKTMLDYDVIGFQTQEWLESFLHYCSKELGAEVDEETGRISYEGRTVLAKAYPIGIDWDHFQAQ
GETGEARQAQQRLMSSVRHRTAMIGVDRLDYSKGLPERIDGIGRFFDQHPDRVRDLVFIQIAPPSREDVESYQQIRTVLE
QKSGQINGARSEVDVVPIRYVNRGYSHAELYGFFRASKIGLVTPLRDGMNLVAKEYLAAQDPEDPGVLILSRFAGAAAQL
PDAVLVNPHSPDDLSHAIKKALDMPLDERKRRYEGTIGTVRDDNVQDWTEAFTNDLASVPI

Sequences:

>Translated_461_residues
MSRLVVISNRVAVPKARGAAGAQGGLAGALNSALKKFGGIWFGWSGQESEDRTGNINLQRNDGVTTATIDLSTRDIDEYY
NGYANSTLWPLFHYRLDLTEYERETGKGYERVNERFAECVTPLIEEDDLVWVHDYHLLPLGERLRSRGLKNRIGFFLHIP
WPPTRLFVSLPFHDRLVKTMLDYDVIGFQTQEWLESFLHYCSKELGAEVDEETGRISYEGRTVLAKAYPIGIDWDHFQAQ
GETGEARQAQQRLMSSVRHRTAMIGVDRLDYSKGLPERIDGIGRFFDQHPDRVRDLVFIQIAPPSREDVESYQQIRTVLE
QKSGQINGARSEVDVVPIRYVNRGYSHAELYGFFRASKIGLVTPLRDGMNLVAKEYLAAQDPEDPGVLILSRFAGAAAQL
PDAVLVNPHSPDDLSHAIKKALDMPLDERKRRYEGTIGTVRDDNVQDWTEAFTNDLASVPI
>Mature_460_residues
SRLVVISNRVAVPKARGAAGAQGGLAGALNSALKKFGGIWFGWSGQESEDRTGNINLQRNDGVTTATIDLSTRDIDEYYN
GYANSTLWPLFHYRLDLTEYERETGKGYERVNERFAECVTPLIEEDDLVWVHDYHLLPLGERLRSRGLKNRIGFFLHIPW
PPTRLFVSLPFHDRLVKTMLDYDVIGFQTQEWLESFLHYCSKELGAEVDEETGRISYEGRTVLAKAYPIGIDWDHFQAQG
ETGEARQAQQRLMSSVRHRTAMIGVDRLDYSKGLPERIDGIGRFFDQHPDRVRDLVFIQIAPPSREDVESYQQIRTVLEQ
KSGQINGARSEVDVVPIRYVNRGYSHAELYGFFRASKIGLVTPLRDGMNLVAKEYLAAQDPEDPGVLILSRFAGAAAQLP
DAVLVNPHSPDDLSHAIKKALDMPLDERKRRYEGTIGTVRDDNVQDWTEAFTNDLASVPI

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=459, Percent_Identity=43.1372549019608, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI115533560, Length=351, Percent_Identity=30.7692307692308, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI115533558, Length=351, Percent_Identity=30.7692307692308, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=414, Percent_Identity=29.4685990338164, Blast_Score=148, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=34.9036402569593, Blast_Score=296, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6323917, Length=379, Percent_Identity=27.4406332453826, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6323537, Length=464, Percent_Identity=26.5086206896552, Blast_Score=192, Evalue=8e-50,
Organism=Saccharomyces cerevisiae, GI6320279, Length=398, Percent_Identity=26.3819095477387, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI19920676, Length=489, Percent_Identity=33.5378323108384, Blast_Score=278, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52156; Mature: 52025

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVVISNRVAVPKARGAAGAQGGLAGALNSALKKFGGIWFGWSGQESEDRTGNINLQR
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEE
NDGVTTATIDLSTRDIDEYYNGYANSTLWPLFHYRLDLTEYERETGKGYERVNERFAECV
CCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHEECHHHHHHHHCCHHHHHHHHHHHHH
TPLIEEDDLVWVHDYHLLPLGERLRSRGLKNRIGFFLHIPWPPTRLFVSLPFHDRLVKTM
HHHCCCCCEEEEECEEECCHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCHHHHHHHHH
LDYDVIGFQTQEWLESFLHYCSKELGAEVDEETGRISYEGRTVLAKAYPIGIDWDHFQAQ
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHCCC
GETGEARQAQQRLMSSVRHRTAMIGVDRLDYSKGLPERIDGIGRFFDQHPDRVRDLVFIQ
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHCEEEEE
IAPPSREDVESYQQIRTVLEQKSGQINGARSEVDVVPIRYVNRGYSHAELYGFFRASKIG
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHCCCC
LVTPLRDGMNLVAKEYLAAQDPEDPGVLILSRFAGAAAQLPDAVLVNPHSPDDLSHAIKK
EECCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCHHHHCCCEEEECCCCCHHHHHHHHH
ALDMPLDERKRRYEGTIGTVRDDNVQDWTEAFTNDLASVPI
HHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRLVVISNRVAVPKARGAAGAQGGLAGALNSALKKFGGIWFGWSGQESEDRTGNINLQR
CCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEECCCCCCCCCCCCCEEEEE
NDGVTTATIDLSTRDIDEYYNGYANSTLWPLFHYRLDLTEYERETGKGYERVNERFAECV
CCCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHEECHHHHHHHHCCHHHHHHHHHHHHH
TPLIEEDDLVWVHDYHLLPLGERLRSRGLKNRIGFFLHIPWPPTRLFVSLPFHDRLVKTM
HHHCCCCCEEEEECEEECCHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCHHHHHHHHH
LDYDVIGFQTQEWLESFLHYCSKELGAEVDEETGRISYEGRTVLAKAYPIGIDWDHFQAQ
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEEEEECCCCHHHHCCC
GETGEARQAQQRLMSSVRHRTAMIGVDRLDYSKGLPERIDGIGRFFDQHPDRVRDLVFIQ
CCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCCCCHHHCEEEEE
IAPPSREDVESYQQIRTVLEQKSGQINGARSEVDVVPIRYVNRGYSHAELYGFFRASKIG
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHCCCC
LVTPLRDGMNLVAKEYLAAQDPEDPGVLILSRFAGAAAQLPDAVLVNPHSPDDLSHAIKK
EECCCHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCHHHHCCCEEEECCCCCHHHHHHHHH
ALDMPLDERKRRYEGTIGTVRDDNVQDWTEAFTNDLASVPI
HHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]