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The map label for this gene is yrkH [H]

Identifier: 85373736

GI number: 85373736

Start: 944040

End: 945392

Strand: Reverse

Name: yrkH [H]

Synonym: ELI_04545

Alternate gene names: 85373736

Gene position: 945392-944040 (Counterclockwise)

Preceding gene: 85373737

Following gene: 85373733

Centisome position: 30.97

GC content: 62.16

Gene sequence:

>1353_bases
ATGTTTCTGGAGAAAATCAAAACCCCCGGTCTCTCACACCTTTCCTACATCATCGGCTCGGGCGGAAAGGCAGCGGTCAT
CGATCCGCGCCGGGACTGCGAGGTCTATGTCGAAAAGGCCCGGGCCGAAGGGCTGGAGATAACGCATATCTTCGAAACCC
ATCGCAATGAGGATCTGCTCAGCGGCTCGCCGATCTTGGCCAATTTGACAGGAGCCACGGTCTATCACGGGCCCAACGCG
GCAGGCGAAGTCATCTTTGCCAGGACCGCGCGCGAAGGCGATTGTTTCGAGCTGGGTCAATTGAAGATCGAAGTGCTTGA
AACACCTGGACACACCGACGATCATCTCGCTTTCGTCATCCATGACAGCGAATATTCCGAGGGGCCGGTCGGCGTCTTTA
CCGGCGATGCCCTGTTCGTCGGCGATGTCGGGCGCACCGATTTCTATCCCGAGCGCAAGGAAGAAGTCGCGGGCCTTCTA
TTCGACTCCCTGCAGAAACTTTGCGGCCTCGGCGATCAGGCGATTATCTACCCGGCGCATGGTGCGGGCTCGGTCTGCGG
GTCGGGAATGGCGGACCGGGAATTCTCGACAATCGGCCACGAGCGGCGCAACAACCCGCGCCTGCGCATCTCCGACCGCG
ACGAGTTCATCGAGGCGAAGGTAGCAGAGCACCACTACCAGCCGCCCTATTTCCGGCTGATGGAGCGGCTCAACCTTGCA
GGGTCCGATGCCGCGCCGCGCGTCATGCGGCCAAGGCGTCTGGGGCTGGAAGACCTTAGCGAGAGCGGCGCCGATCACCT
GGTCGATGTGCGCGAGCCGCTCGCCTACGCTGCCGGCCATCTGCCGGGTGCCATGTGCCTTCCGGTTGGAATGATACCGG
CCTTTGCCGGATGGTTCCTCGGTGAAGGCGACAGGATCGCCCTCATTGCTTCCGAGGAAGGCCAGCTAGCCCAGGCCATG
GCCCATCTGGTGCGCATCGGTCTCGATAATGTTGTGGGCGGCTATGTCGGCGTGGTTCCAGCCGCCGCACAGGGCAAAGC
GATGCGCAGCATTCCCATGATTGGCACCGAGGAAGTCGCGCGCAGGCTCGATCAAGACAGCGGCGAGTGGACCTTGCTCG
ACGTGCGCGACGCGGACGAACGGCAAGAAGCAAGCATCGACGGCTCAGGACATATCTATGTCGGTGAGCTCAACACGCGG
TGGGAAGAGCTCGACCGCGACCGCCACTACACGCTGATGTGCGCGAGCGGCATGCGGGCGAGCGTTGCGGCGGGTTGGCT
GGCCAGCAAGGGTTTCGAGCACCTCGATATCTATCTGGGCTCTATGGGGGCGTGGACAAACGCCTCCGGCTGA

Upstream 100 bases:

>100_bases
CCATCAAGGCCGAACTCGGGATCGATTAAACGTCGTAACATTTTTCAATCTGTAACGAGCCCGAACGTTACAGACTTTGA
GCAACAGACAAGGAAGTCGC

Downstream 100 bases:

>100_bases
AGCGCCAAAATCTTGAAAAGCAAGCCAAAATCTTTTGCGAGATTTTGCTCGTATGTGGTAGGTGAGCTTGCTCTTCGCTC
AACGAGCAGCGCGACCGGGC

Product: hydrolase

Products: glutathione; 2-hydroxy carboxylate

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MFLEKIKTPGLSHLSYIIGSGGKAAVIDPRRDCEVYVEKARAEGLEITHIFETHRNEDLLSGSPILANLTGATVYHGPNA
AGEVIFARTAREGDCFELGQLKIEVLETPGHTDDHLAFVIHDSEYSEGPVGVFTGDALFVGDVGRTDFYPERKEEVAGLL
FDSLQKLCGLGDQAIIYPAHGAGSVCGSGMADREFSTIGHERRNNPRLRISDRDEFIEAKVAEHHYQPPYFRLMERLNLA
GSDAAPRVMRPRRLGLEDLSESGADHLVDVREPLAYAAGHLPGAMCLPVGMIPAFAGWFLGEGDRIALIASEEGQLAQAM
AHLVRIGLDNVVGGYVGVVPAAAQGKAMRSIPMIGTEEVARRLDQDSGEWTLLDVRDADERQEASIDGSGHIYVGELNTR
WEELDRDRHYTLMCASGMRASVAAGWLASKGFEHLDIYLGSMGAWTNASG

Sequences:

>Translated_450_residues
MFLEKIKTPGLSHLSYIIGSGGKAAVIDPRRDCEVYVEKARAEGLEITHIFETHRNEDLLSGSPILANLTGATVYHGPNA
AGEVIFARTAREGDCFELGQLKIEVLETPGHTDDHLAFVIHDSEYSEGPVGVFTGDALFVGDVGRTDFYPERKEEVAGLL
FDSLQKLCGLGDQAIIYPAHGAGSVCGSGMADREFSTIGHERRNNPRLRISDRDEFIEAKVAEHHYQPPYFRLMERLNLA
GSDAAPRVMRPRRLGLEDLSESGADHLVDVREPLAYAAGHLPGAMCLPVGMIPAFAGWFLGEGDRIALIASEEGQLAQAM
AHLVRIGLDNVVGGYVGVVPAAAQGKAMRSIPMIGTEEVARRLDQDSGEWTLLDVRDADERQEASIDGSGHIYVGELNTR
WEELDRDRHYTLMCASGMRASVAAGWLASKGFEHLDIYLGSMGAWTNASG
>Mature_450_residues
MFLEKIKTPGLSHLSYIIGSGGKAAVIDPRRDCEVYVEKARAEGLEITHIFETHRNEDLLSGSPILANLTGATVYHGPNA
AGEVIFARTAREGDCFELGQLKIEVLETPGHTDDHLAFVIHDSEYSEGPVGVFTGDALFVGDVGRTDFYPERKEEVAGLL
FDSLQKLCGLGDQAIIYPAHGAGSVCGSGMADREFSTIGHERRNNPRLRISDRDEFIEAKVAEHHYQPPYFRLMERLNLA
GSDAAPRVMRPRRLGLEDLSESGADHLVDVREPLAYAAGHLPGAMCLPVGMIPAFAGWFLGEGDRIALIASEEGQLAQAM
AHLVRIGLDNVVGGYVGVVPAAAQGKAMRSIPMIGTEEVARRLDQDSGEWTLLDVRDADERQEASIDGSGHIYVGELNTR
WEELDRDRHYTLMCASGMRASVAAGWLASKGFEHLDIYLGSMGAWTNASG

Specific function: Unknown

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI21703352, Length=220, Percent_Identity=30.4545454545455, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI116642887, Length=221, Percent_Identity=30.316742081448, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI41327741, Length=229, Percent_Identity=27.9475982532751, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI94538322, Length=146, Percent_Identity=32.8767123287671, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI94538320, Length=146, Percent_Identity=32.8767123287671, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1787158, Length=206, Percent_Identity=28.6407766990291, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538952, Length=213, Percent_Identity=32.3943661971831, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330176, Length=236, Percent_Identity=31.3559322033898, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00753 Lactamase_B; PF00581 Rhodanese [H]

EC number: 3.1.2.6

Molecular weight: Translated: 48941; Mature: 48941

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEKIKTPGLSHLSYIIGSGGKAAVIDPRRDCEVYVEKARAEGLEITHIFETHRNEDLL
CCHHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEHHCCCCCCC
SGSPILANLTGATVYHGPNAAGEVIFARTAREGDCFELGQLKIEVLETPGHTDDHLAFVI
CCCCEEEECCCCEEEECCCCCCCEEEEECCCCCCCEEECCEEEEEEECCCCCCCEEEEEE
HDSEYSEGPVGVFTGDALFVGDVGRTDFYPERKEEVAGLLFDSLQKLCGLGDQAIIYPAH
ECCCCCCCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GAGSVCGSGMADREFSTIGHERRNNPRLRISDRDEFIEAKVAEHHYQPPYFRLMERLNLA
CCCCHHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCC
GSDAAPRVMRPRRLGLEDLSESGADHLVDVREPLAYAAGHLPGAMCLPVGMIPAFAGWFL
CCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GEGDRIALIASEEGQLAQAMAHLVRIGLDNVVGGYVGVVPAAAQGKAMRSIPMIGTEEVA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHCCCCCCHHHHH
RRLDQDSGEWTLLDVRDADERQEASIDGSGHIYVGELNTRWEELDRDRHYTLMCASGMRA
HHHCCCCCCEEEEECCCCCCHHHCCCCCCCCEEECCCCCHHHHHCCCCCEEEEEECCCCH
SVAAGWLASKGFEHLDIYLGSMGAWTNASG
HHHHHHHHHCCCCEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MFLEKIKTPGLSHLSYIIGSGGKAAVIDPRRDCEVYVEKARAEGLEITHIFETHRNEDLL
CCHHHHCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHCCCCEEEEEEHHCCCCCCC
SGSPILANLTGATVYHGPNAAGEVIFARTAREGDCFELGQLKIEVLETPGHTDDHLAFVI
CCCCEEEECCCCEEEECCCCCCCEEEEECCCCCCCEEECCEEEEEEECCCCCCCEEEEEE
HDSEYSEGPVGVFTGDALFVGDVGRTDFYPERKEEVAGLLFDSLQKLCGLGDQAIIYPAH
ECCCCCCCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECC
GAGSVCGSGMADREFSTIGHERRNNPRLRISDRDEFIEAKVAEHHYQPPYFRLMERLNLA
CCCCHHCCCCCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHCCC
GSDAAPRVMRPRRLGLEDLSESGADHLVDVREPLAYAAGHLPGAMCLPVGMIPAFAGWFL
CCCCCCCCCCCHHCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
GEGDRIALIASEEGQLAQAMAHLVRIGLDNVVGGYVGVVPAAAQGKAMRSIPMIGTEEVA
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHCCCCCCHHHHH
RRLDQDSGEWTLLDVRDADERQEASIDGSGHIYVGELNTRWEELDRDRHYTLMCASGMRA
HHHCCCCCCEEEEECCCCCCHHHCCCCCCCCEEECCCCCHHHHHCCCCCEEEEEECCCCH
SVAAGWLASKGFEHLDIYLGSMGAWTNASG
HHHHHHHHHCCCCEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-(2-hydroxyacyl)glutathione; H2O

Specific reaction: S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]