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The map label for this gene is silB [H]

Identifier: 85373727

GI number: 85373727

Start: 931780

End: 933288

Strand: Direct

Name: silB [H]

Synonym: ELI_04500

Alternate gene names: 85373727

Gene position: 931780-933288 (Clockwise)

Preceding gene: 85373726

Following gene: 85373728

Centisome position: 30.53

GC content: 64.81

Gene sequence:

>1509_bases
ATGAACTCCGCGCTCGAACGATTCACACCGCGCCAGCGCATGTATGCGGCTGGACTTGGTATTGCTCTGGTCAGTCTGTT
GGCGGGCTACGGTCTCTCAATGCTCGGCAGCGAGAGCGGCGGCGATGCAGCGACAGATACGGGCTGCGAGGACGTGCTCT
ATTGGTACGATCCGATGGTCCCTGACCAGCGCTTCGACGAGCCGGGCAAATCTCCTTTCATGGACATGCAGCTGGTCCCC
AAATGCGCGGGCGGCGACGCGCAGGGCGCCGGGGGTGTGAGTATCGATTCGACGCTCGTCCAGAATTTCGGGATCCGGAC
TGCTGAAGCGGAATATGGCGTGCTGGAGCCTGAAATCAGCGTCACCGGCACGCTCGCATATAACGGCAGCGAGATGGCGA
TCGTTCAGCCGCGCGCCGGCGGCTATGTTCAGCGGACCTACGGCCATGCACCGCAGGACCTGATTGCCAGAGGCGCTCCG
ATCGTCGACATTCTTGTGCCCGAATGGGGCGGCGCACAAAACGAGTATCTTGCTGTTGCCGCAACCGGCGACGAAGCGTT
GACCCGGGCCGCCCGCGAGCGCCTGCGGCTGCTTGGCATGCCGGACTCGATGATCGCCTCGGTCACCCGAAGCGGAAGAC
CGCAAACGATCATCACCGTCACAGCACCGCAATCGGGCGCGATCACGTCGCTCGCAGTAAGGCCGGGCATGACCGTGGCG
GCGGGCCAATCGCTGGCCGAGATCAGCGGGTACAGTCCGATCTGGCTCGAGGCGGCGGTACCCGAGGCACTGGCTGGCAA
CGCGCGTGTCGGGCAGCCGGTGAGCGCCACGCTCACTGCCTTTCCCGGCGAACGGTTTGCCGGACGCATCATCGCCATTC
TGCCGAGCGCGCAAGATGCGAGCCGCACGATAACCGTGCGGGCAGCGATGCCCAATCCGGGCCTGCGGCTCAAGCCGGGA
ATGTTTGCCCAAGTGATGCTCGCGCCTGAACGGCGCGAAGCGCTGCTGGTCCCTTCGGAAGCTGTCATTCGCACCGGCGA
GCGGACCCTGGCGATGATCGCGCAGGGAGGTGGCGGCTATCGGCCCGCCGAAATCCGTATCGGCCGCGAAGCGAACGGAC
GGACCGAAGTCCTTGCCGGGCTGGCTGCAGGAGAGAGAGTGGTCACCTCGGGCCAGTTCCTGCTCGATTCCGAAGCCAGC
CTTGCGGGCATCGACGTGCGTCCCGTCGATCAGGCACCATCCGGTGCGAGCGAAGACCGAGGGTCGGACGATGCAGATGA
ACCGCAGACTTATCGCGCGACCGGGACGATCGAGCGCATAAACGCCAACAGCGTGACCTTGCGCCACGGTCCGGTGCCTG
CGCTCGAATGGCCGGCAATGACCATGCCCTTTGCCAGCGAAGGACCGGAACAGCTGCGCGGTTTTGCGCGCGGCGACCGG
GTCTCCTTCACATTTGTGCAAGCCAGTACGGGGCCGCGCATCGTCTCGATCCGGAAAATCGGCCAATGA

Upstream 100 bases:

>100_bases
AATCCGCGCTCGCGCTGCTCGAACTGGAGATACTCGAGCGCGAACAGGCAGCTGTCGAGGCGGCGGTGAGGCTGCGACTG
ACCTACGGGGAGAGCAGGCC

Downstream 100 bases:

>100_bases
TCGCGCGCATCATCGATAGCTCGATCGCCAACCGCTTTTTCGTGGTGCTGGCAGCGATCGGGTTGGCGCTGGCCGGTTTC
TGGGCGGTACGGACGACGCC

Product: heavy metal RND efflux membrane fusion protein, CzcB family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MNSALERFTPRQRMYAAGLGIALVSLLAGYGLSMLGSESGGDAATDTGCEDVLYWYDPMVPDQRFDEPGKSPFMDMQLVP
KCAGGDAQGAGGVSIDSTLVQNFGIRTAEAEYGVLEPEISVTGTLAYNGSEMAIVQPRAGGYVQRTYGHAPQDLIARGAP
IVDILVPEWGGAQNEYLAVAATGDEALTRAARERLRLLGMPDSMIASVTRSGRPQTIITVTAPQSGAITSLAVRPGMTVA
AGQSLAEISGYSPIWLEAAVPEALAGNARVGQPVSATLTAFPGERFAGRIIAILPSAQDASRTITVRAAMPNPGLRLKPG
MFAQVMLAPERREALLVPSEAVIRTGERTLAMIAQGGGGYRPAEIRIGREANGRTEVLAGLAAGERVVTSGQFLLDSEAS
LAGIDVRPVDQAPSGASEDRGSDDADEPQTYRATGTIERINANSVTLRHGPVPALEWPAMTMPFASEGPEQLRGFARGDR
VSFTFVQASTGPRIVSIRKIGQ

Sequences:

>Translated_502_residues
MNSALERFTPRQRMYAAGLGIALVSLLAGYGLSMLGSESGGDAATDTGCEDVLYWYDPMVPDQRFDEPGKSPFMDMQLVP
KCAGGDAQGAGGVSIDSTLVQNFGIRTAEAEYGVLEPEISVTGTLAYNGSEMAIVQPRAGGYVQRTYGHAPQDLIARGAP
IVDILVPEWGGAQNEYLAVAATGDEALTRAARERLRLLGMPDSMIASVTRSGRPQTIITVTAPQSGAITSLAVRPGMTVA
AGQSLAEISGYSPIWLEAAVPEALAGNARVGQPVSATLTAFPGERFAGRIIAILPSAQDASRTITVRAAMPNPGLRLKPG
MFAQVMLAPERREALLVPSEAVIRTGERTLAMIAQGGGGYRPAEIRIGREANGRTEVLAGLAAGERVVTSGQFLLDSEAS
LAGIDVRPVDQAPSGASEDRGSDDADEPQTYRATGTIERINANSVTLRHGPVPALEWPAMTMPFASEGPEQLRGFARGDR
VSFTFVQASTGPRIVSIRKIGQ
>Mature_502_residues
MNSALERFTPRQRMYAAGLGIALVSLLAGYGLSMLGSESGGDAATDTGCEDVLYWYDPMVPDQRFDEPGKSPFMDMQLVP
KCAGGDAQGAGGVSIDSTLVQNFGIRTAEAEYGVLEPEISVTGTLAYNGSEMAIVQPRAGGYVQRTYGHAPQDLIARGAP
IVDILVPEWGGAQNEYLAVAATGDEALTRAARERLRLLGMPDSMIASVTRSGRPQTIITVTAPQSGAITSLAVRPGMTVA
AGQSLAEISGYSPIWLEAAVPEALAGNARVGQPVSATLTAFPGERFAGRIIAILPSAQDASRTITVRAAMPNPGLRLKPG
MFAQVMLAPERREALLVPSEAVIRTGERTLAMIAQGGGGYRPAEIRIGREANGRTEVLAGLAAGERVVTSGQFLLDSEAS
LAGIDVRPVDQAPSGASEDRGSDDADEPQTYRATGTIERINANSVTLRHGPVPALEWPAMTMPFASEGPEQLRGFARGDR
VSFTFVQASTGPRIVSIRKIGQ

Specific function: Component of the sil cation efflux system that confers resistance to silver. May be part of a three-component cation/proton antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=360, Percent_Identity=39.4444444444444, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 52781; Mature: 52781

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSALERFTPRQRMYAAGLGIALVSLLAGYGLSMLGSESGGDAATDTGCEDVLYWYDPMV
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEECCCCC
PDQRFDEPGKSPFMDMQLVPKCAGGDAQGAGGVSIDSTLVQNFGIRTAEAEYGVLEPEIS
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCEECCCEE
VTGTLAYNGSEMAIVQPRAGGYVQRTYGHAPQDLIARGAPIVDILVPEWGGAQNEYLAVA
EEEEEEECCCEEEEECCCCCCEEEECCCCCCHHHHHCCCCEEEEEECCCCCCCCCEEEEE
ATGDEALTRAARERLRLLGMPDSMIASVTRSGRPQTIITVTAPQSGAITSLAVRPGMTVA
ECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCEEE
AGQSLAEISGYSPIWLEAAVPEALAGNARVGQPVSATLTAFPGERFAGRIIAILPSAQDA
CCCCHHHHCCCCCEEEEECCCHHHCCCCCCCCCCEEEEEECCCCHHCCEEEEEECCCCCC
SRTITVRAAMPNPGLRLKPGMFAQVMLAPERREALLVPSEAVIRTGERTLAMIAQGGGGY
CEEEEEEEECCCCCCEECCCCEEEEEECCCCCCEEECCCHHHHHCCCCEEEEEEECCCCC
RPAEIRIGREANGRTEVLAGLAAGERVVTSGQFLLDSEASLAGIDVRPVDQAPSGASEDR
CCEEEEECCCCCCHHHHHHHHHCCCEEEECCCEEEECCCCEEECEEEECCCCCCCCCCCC
GSDDADEPQTYRATGTIERINANSVTLRHGPVPALEWPAMTMPFASEGPEQLRGFARGDR
CCCCCCCCCEEEECCCEEEECCCEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHCCCCE
VSFTFVQASTGPRIVSIRKIGQ
EEEEEEEECCCCEEEEEEECCC
>Mature Secondary Structure
MNSALERFTPRQRMYAAGLGIALVSLLAGYGLSMLGSESGGDAATDTGCEDVLYWYDPMV
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEECCCCC
PDQRFDEPGKSPFMDMQLVPKCAGGDAQGAGGVSIDSTLVQNFGIRTAEAEYGVLEPEIS
CCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHCCCEECCCCCCEECCCEE
VTGTLAYNGSEMAIVQPRAGGYVQRTYGHAPQDLIARGAPIVDILVPEWGGAQNEYLAVA
EEEEEEECCCEEEEECCCCCCEEEECCCCCCHHHHHCCCCEEEEEECCCCCCCCCEEEEE
ATGDEALTRAARERLRLLGMPDSMIASVTRSGRPQTIITVTAPQSGAITSLAVRPGMTVA
ECCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCEEE
AGQSLAEISGYSPIWLEAAVPEALAGNARVGQPVSATLTAFPGERFAGRIIAILPSAQDA
CCCCHHHHCCCCCEEEEECCCHHHCCCCCCCCCCEEEEEECCCCHHCCEEEEEECCCCCC
SRTITVRAAMPNPGLRLKPGMFAQVMLAPERREALLVPSEAVIRTGERTLAMIAQGGGGY
CEEEEEEEECCCCCCEECCCCEEEEEECCCCCCEEECCCHHHHHCCCCEEEEEEECCCCC
RPAEIRIGREANGRTEVLAGLAAGERVVTSGQFLLDSEASLAGIDVRPVDQAPSGASEDR
CCEEEEECCCCCCHHHHHHHHHCCCEEEECCCEEEECCCCEEECEEEECCCCCCCCCCCC
GSDDADEPQTYRATGTIERINANSVTLRHGPVPALEWPAMTMPFASEGPEQLRGFARGDR
CCCCCCCCCEEEECCCEEEECCCEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHCCCCE
VSFTFVQASTGPRIVSIRKIGQ
EEEEEEEECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9930866 [H]