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The map label for this gene is scrR [H]

Identifier: 85373677

GI number: 85373677

Start: 880753

End: 881790

Strand: Direct

Name: scrR [H]

Synonym: ELI_04250

Alternate gene names: 85373677

Gene position: 880753-881790 (Clockwise)

Preceding gene: 85373676

Following gene: 85373683

Centisome position: 28.85

GC content: 60.98

Gene sequence:

>1038_bases
ATGACAGATCCCAAGCCAACCGACCGGGCCGATGCCGCCAAGCCAGTCCGCACGATCACGGATCTGGCGCGCATAGCTGG
CGTGTCGGCAGGAACTGTCTCGCGAGCGCTGTCAGGCAACAGTCTGGTCAACACCAAGACCCGCGAGAAGATCGAAGCCA
TTGCGCGCGAACATGGCTTTCGTCCGAACCAGATGGCCAGCCGACTACGCCGGCAAAAGACCGGTGTCATTGGCGTGGCC
ATCCCGCTGGGTCACGACGTCCAACAACAGATTTCCGACACTTTCTTCATGACCCTGCTCGGCTTTCTTGCCGACGAGCT
GACTGCCAAGGGTTATGACCTGATGCTGCGCCGCGTCGTGCCGAGCAATGACGAGGACTGGCTGGATCGCTTCATCGGTT
CCGGCATGATCGACGGCGTCGTCGTGATCGGACAATCCGACCAGTTCGAGCGGATCGAGGAGGTGGCTGACGGCTACCTG
CCCATGGTCGTGTGGGGAAACCACCAGGAAGGCCAAAGGCATTGCGTCGTCGGCACCGATAACCGGCTGGGCGGAAAGCT
CGCGGCTGAACGCCTGATCGCGGCTGGCGCCACGAGCTTGGCATTTCTTGGTGACACCGACCCCATCGAGTTCGCAGCCC
GGTTTGGCGGGGCCAAGGAAGTTGCGGACAAGCGCAACGTGCCGATACGTGCGTTGCCAACGCATCTTTCGCCCGGCCAG
GTTTCGTCCGAGATCGCAGCGCATCTCGAAGTGATCCAGCATGAAGCCGATGGGGTATTTGCTGCGACTGATACGATCGC
CGCCACTTGCCTGCAGGAGATGCGAACCAAGGGCATCGTTGTGCCAAATCACGTCAAACTTGTCGGGTTCGACGATTTGC
CTATCGCCAGCCAGACAATGCCGCCCCTGACCACAATCAAACAGGACATTGCGGCTGGCGCGAAGGGACTGGTCGACCTG
TTGCTGCGCCGGATGGCGGGCGAAGATACTGAAAGCCTTGTCCTGCCTCCCCATCTGGTGGCGAGGCAGACGGCTTAG

Upstream 100 bases:

>100_bases
GGAGCTCTGATGCTAGTCGGAGCCATTGCTACCTTGTTCGTTGATGCAGGACATCGCGTCCCGCGCGAACAAAAGCTCAT
GGCAGGATAGAGACAGCAGC

Downstream 100 bases:

>100_bases
CTCTTAGCGGTATCTTGGACCGCGCCCGCCCCTGGGAGTCGCCTATGGAGGCAGCGGCGGGATAACAGATCACTGTCGGC
TCGACTCGCCAGAAGATCGA

Product: transcriptional regulator, LacI family protein

Products: NA

Alternate protein names: Scr operon regulatory protein [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MTDPKPTDRADAAKPVRTITDLARIAGVSAGTVSRALSGNSLVNTKTREKIEAIAREHGFRPNQMASRLRRQKTGVIGVA
IPLGHDVQQQISDTFFMTLLGFLADELTAKGYDLMLRRVVPSNDEDWLDRFIGSGMIDGVVVIGQSDQFERIEEVADGYL
PMVVWGNHQEGQRHCVVGTDNRLGGKLAAERLIAAGATSLAFLGDTDPIEFAARFGGAKEVADKRNVPIRALPTHLSPGQ
VSSEIAAHLEVIQHEADGVFAATDTIAATCLQEMRTKGIVVPNHVKLVGFDDLPIASQTMPPLTTIKQDIAAGAKGLVDL
LLRRMAGEDTESLVLPPHLVARQTA

Sequences:

>Translated_345_residues
MTDPKPTDRADAAKPVRTITDLARIAGVSAGTVSRALSGNSLVNTKTREKIEAIAREHGFRPNQMASRLRRQKTGVIGVA
IPLGHDVQQQISDTFFMTLLGFLADELTAKGYDLMLRRVVPSNDEDWLDRFIGSGMIDGVVVIGQSDQFERIEEVADGYL
PMVVWGNHQEGQRHCVVGTDNRLGGKLAAERLIAAGATSLAFLGDTDPIEFAARFGGAKEVADKRNVPIRALPTHLSPGQ
VSSEIAAHLEVIQHEADGVFAATDTIAATCLQEMRTKGIVVPNHVKLVGFDDLPIASQTMPPLTTIKQDIAAGAKGLVDL
LLRRMAGEDTESLVLPPHLVARQTA
>Mature_344_residues
TDPKPTDRADAAKPVRTITDLARIAGVSAGTVSRALSGNSLVNTKTREKIEAIAREHGFRPNQMASRLRRQKTGVIGVAI
PLGHDVQQQISDTFFMTLLGFLADELTAKGYDLMLRRVVPSNDEDWLDRFIGSGMIDGVVVIGQSDQFERIEEVADGYLP
MVVWGNHQEGQRHCVVGTDNRLGGKLAAERLIAAGATSLAFLGDTDPIEFAARFGGAKEVADKRNVPIRALPTHLSPGQV
SSEIAAHLEVIQHEADGVFAATDTIAATCLQEMRTKGIVVPNHVKLVGFDDLPIASQTMPPLTTIKQDIAAGAKGLVDLL
LRRMAGEDTESLVLPPHLVARQTA

Specific function: Negative regulator of scrB expression [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=29.7619047619048, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789068, Length=321, Percent_Identity=24.9221183800623, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790369, Length=331, Percent_Identity=25.3776435045317, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790194, Length=338, Percent_Identity=24.8520710059172, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786540, Length=339, Percent_Identity=27.1386430678466, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=22.8668941979522, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787906, Length=347, Percent_Identity=27.9538904899135, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787948, Length=345, Percent_Identity=24.9275362318841, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790689, Length=331, Percent_Identity=25.6797583081571, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787580, Length=336, Percent_Identity=24.4047619047619, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=23.841059602649, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI48994940, Length=302, Percent_Identity=24.5033112582781, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1789456, Length=309, Percent_Identity=23.3009708737864, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37148; Mature: 37017

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPKPTDRADAAKPVRTITDLARIAGVSAGTVSRALSGNSLVNTKTREKIEAIAREHGF
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
RPNQMASRLRRQKTGVIGVAIPLGHDVQQQISDTFFMTLLGFLADELTAKGYDLMLRRVV
CHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
PSNDEDWLDRFIGSGMIDGVVVIGQSDQFERIEEVADGYLPMVVWGNHQEGQRHCVVGTD
CCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECC
NRLGGKLAAERLIAAGATSLAFLGDTDPIEFAARFGGAKEVADKRNVPIRALPTHLSPGQ
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCH
VSSEIAAHLEVIQHEADGVFAATDTIAATCLQEMRTKGIVVPNHVKLVGFDDLPIASQTM
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
PPLTTIKQDIAAGAKGLVDLLLRRMAGEDTESLVLPPHLVARQTA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHCCCC
>Mature Secondary Structure 
TDPKPTDRADAAKPVRTITDLARIAGVSAGTVSRALSGNSLVNTKTREKIEAIAREHGF
CCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
RPNQMASRLRRQKTGVIGVAIPLGHDVQQQISDTFFMTLLGFLADELTAKGYDLMLRRVV
CHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
PSNDEDWLDRFIGSGMIDGVVVIGQSDQFERIEEVADGYLPMVVWGNHQEGQRHCVVGTD
CCCCHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEECC
NRLGGKLAAERLIAAGATSLAFLGDTDPIEFAARFGGAKEVADKRNVPIRALPTHLSPGQ
CCCCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHCCCHHHHHHHCCCCEEEECCCCCCCH
VSSEIAAHLEVIQHEADGVFAATDTIAATCLQEMRTKGIVVPNHVKLVGFDDLPIASQTM
HHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCC
PPLTTIKQDIAAGAKGLVDLLLRRMAGEDTESLVLPPHLVARQTA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA