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The map label for this gene is aspS

Identifier: 85373620

GI number: 85373620

Start: 817566

End: 819380

Strand: Direct

Name: aspS

Synonym: ELI_03965

Alternate gene names: 85373620

Gene position: 817566-819380 (Clockwise)

Preceding gene: 85373616

Following gene: 85373624

Centisome position: 26.78

GC content: 61.98

Gene sequence:

>1815_bases
ATGCACGCCTATCGTACCCACAACTGCGCAGCGCTGAGCAAGAAAAATGTCGGCGAAACCGTCCGCCTGTCGGGCTGGGT
GCACAACAAGCGCGACCACGGGGGCGTGCTGTTCGTCGATCTGCGCGACCATTACGGCATCACCCAGATCGTCGCGGACG
AAGACAGCGCGGCGCTGCCGGTACTCGACAAGCTCAAGCTGGAGAGCGTCGTCACCATCGACGGCGACGTGAAGGCGCGC
GATGCCGAGGCGGTCAATCCGAACCTGCCGACCGGCGAGATCGAAGTCTTCGCCCGCTCGGTCGAGATCCAGAGCCGCGC
CGAGGAACTGCCGCTGATCGTCAATTCGGCGGAGGACTATCCCGAGGATGTGCGCCTCAAGTACCGCTTCGTCGACCTGC
GGCGCGAACGTCTGCACCGCAACATCATGCTGCGGAGCCAGGTCATCACCTCGATCCGCAACCGCATGACGGCGCAGGGC
TTCACCGAGTTCCAGACGCCGATCCTAGCCGCCAGCAGCCCCGAGGGCGCGCGCGACTATATCGTGCCGAGCCGGTTGCA
TCCGGGCACCTTCTACGCGCTGCCGCAAGCGCCGCAGATGTTCAAGCAGCTGCTGATGGTGGCCGGTTTCGACCGCTACT
TCCAGATTGCGCCGTGCTTCCGCGATGAAGACCTGCGCGCCGATCGCTCGCCGGAATTCTACCAGCTCGACTTCGAAATG
AGCTTCGTCACGCAGGAAGACGTATTCCAGGCCATCGAGCCGGTGCTGGCGGGCGTGTTCGAAGAGTTCGCGAACGGCAA
GAGTGTGACCAAGTCGGGCGAATTTCCGCGTATTCCCTATGCGGAAGCGATGCTCAAATACGGCACCGACAAGCCCGACC
TGCGCAACCCGCTGGTCATCAGCGACGTGACCGATCATTTCCAGTCGAGCGGTTTCGGCCTGTTCGAAAAGATCGTCGGC
ATTGGTGGCCGCGTCCGCGTGATCCCCGCGCCGAACACGCAAGAGAAGAGCCGCAAGTTCTTCGACGACATGAACGACTG
GGCGCGCAAGGAAGGCTTTGCCGGGCTGGGCTATGTCACGCGCAAGCAGGGCGAATTCGGCGGTCCGATCGCCAAGAACC
ACGGTCCCGAAAACATGCAGAAAATCTACGACGAGCTCGGCCTCGGCGAGAATGACGGCCTGTTCTTCGCCGCGGGCAAG
GAGAAGGACGCCGCGAAGCTCGCCGGTGCCGCCCGTACCCGTGTCGGCGAGGAATTGGGCCTGATAGAGCAGGGCTGTTT
CAAGTTCTGCTGGATCGTCGACTTCCCGATGTTCGAATACGACGAAGAGCTCAAGAAGATCGATTTCAGCCACAATCCCT
TCTCGATGCCGCAGGGCGAGATGGAGGCGCTGGAAAACAAGGATCCGCTCGAGATCCTGGCTTGGCAGTACGACATCGTT
TGCAACGGCTACGAGCTGTCGTCGGGCGCGATCCGGAACCACAAGCCGGAAATCATGTACAAGGCCTTCGAGATTGCCGG
TTACGACCGGGAAACGGTGGACCGTGAGTTCTCCGGCATGATCGAAGCCTTCAAGCTCGGCGCCCCGCCGCACGGTGGCT
CTGCGCCAGGAATCGACCGTATCGTGATGCTGCTGGCCGACGAGCCCAATATCCGCGAAGTGATCGCTTTCCCGCTCAAC
CAGAAGGCGCAGGACCTGATGATGGGCGCACCCAGCCAGGTTCCGCCCAAGGCGCTGCGCGATGTGCATATCCGGACGGT
CGAACCGCCGAAGAAACAGCAGGTCGGCACCCATCTTGTGAACGACGACAGCTAA

Upstream 100 bases:

>100_bases
TGTTAGCGCGCGCGAATTCCGGCTAGGGGCCTTGTCGCCCCGCGCAATCGCGAACGCGCGCGCCCTTAGCCTCATAGCTC
CGAAAATGGAAAGATTTTAG

Downstream 100 bases:

>100_bases
GGCGTTCCCTGGACTGGCGCGCTGCGCGATGCCCCGCCCGAGTAGCGATGATCAACCGCGCTGTCTTGCGGCCCACTGTT
CCAGCTGCGCGTTGCAAGCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MHAYRTHNCAALSKKNVGETVRLSGWVHNKRDHGGVLFVDLRDHYGITQIVADEDSAALPVLDKLKLESVVTIDGDVKAR
DAEAVNPNLPTGEIEVFARSVEIQSRAEELPLIVNSAEDYPEDVRLKYRFVDLRRERLHRNIMLRSQVITSIRNRMTAQG
FTEFQTPILAASSPEGARDYIVPSRLHPGTFYALPQAPQMFKQLLMVAGFDRYFQIAPCFRDEDLRADRSPEFYQLDFEM
SFVTQEDVFQAIEPVLAGVFEEFANGKSVTKSGEFPRIPYAEAMLKYGTDKPDLRNPLVISDVTDHFQSSGFGLFEKIVG
IGGRVRVIPAPNTQEKSRKFFDDMNDWARKEGFAGLGYVTRKQGEFGGPIAKNHGPENMQKIYDELGLGENDGLFFAAGK
EKDAAKLAGAARTRVGEELGLIEQGCFKFCWIVDFPMFEYDEELKKIDFSHNPFSMPQGEMEALENKDPLEILAWQYDIV
CNGYELSSGAIRNHKPEIMYKAFEIAGYDRETVDREFSGMIEAFKLGAPPHGGSAPGIDRIVMLLADEPNIREVIAFPLN
QKAQDLMMGAPSQVPPKALRDVHIRTVEPPKKQQVGTHLVNDDS

Sequences:

>Translated_604_residues
MHAYRTHNCAALSKKNVGETVRLSGWVHNKRDHGGVLFVDLRDHYGITQIVADEDSAALPVLDKLKLESVVTIDGDVKAR
DAEAVNPNLPTGEIEVFARSVEIQSRAEELPLIVNSAEDYPEDVRLKYRFVDLRRERLHRNIMLRSQVITSIRNRMTAQG
FTEFQTPILAASSPEGARDYIVPSRLHPGTFYALPQAPQMFKQLLMVAGFDRYFQIAPCFRDEDLRADRSPEFYQLDFEM
SFVTQEDVFQAIEPVLAGVFEEFANGKSVTKSGEFPRIPYAEAMLKYGTDKPDLRNPLVISDVTDHFQSSGFGLFEKIVG
IGGRVRVIPAPNTQEKSRKFFDDMNDWARKEGFAGLGYVTRKQGEFGGPIAKNHGPENMQKIYDELGLGENDGLFFAAGK
EKDAAKLAGAARTRVGEELGLIEQGCFKFCWIVDFPMFEYDEELKKIDFSHNPFSMPQGEMEALENKDPLEILAWQYDIV
CNGYELSSGAIRNHKPEIMYKAFEIAGYDRETVDREFSGMIEAFKLGAPPHGGSAPGIDRIVMLLADEPNIREVIAFPLN
QKAQDLMMGAPSQVPPKALRDVHIRTVEPPKKQQVGTHLVNDDS
>Mature_604_residues
MHAYRTHNCAALSKKNVGETVRLSGWVHNKRDHGGVLFVDLRDHYGITQIVADEDSAALPVLDKLKLESVVTIDGDVKAR
DAEAVNPNLPTGEIEVFARSVEIQSRAEELPLIVNSAEDYPEDVRLKYRFVDLRRERLHRNIMLRSQVITSIRNRMTAQG
FTEFQTPILAASSPEGARDYIVPSRLHPGTFYALPQAPQMFKQLLMVAGFDRYFQIAPCFRDEDLRADRSPEFYQLDFEM
SFVTQEDVFQAIEPVLAGVFEEFANGKSVTKSGEFPRIPYAEAMLKYGTDKPDLRNPLVISDVTDHFQSSGFGLFEKIVG
IGGRVRVIPAPNTQEKSRKFFDDMNDWARKEGFAGLGYVTRKQGEFGGPIAKNHGPENMQKIYDELGLGENDGLFFAAGK
EKDAAKLAGAARTRVGEELGLIEQGCFKFCWIVDFPMFEYDEELKKIDFSHNPFSMPQGEMEALENKDPLEILAWQYDIV
CNGYELSSGAIRNHKPEIMYKAFEIAGYDRETVDREFSGMIEAFKLGAPPHGGSAPGIDRIVMLLADEPNIREVIAFPLN
QKAQDLMMGAPSQVPPKALRDVHIRTVEPPKKQQVGTHLVNDDS

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=607, Percent_Identity=34.9258649093904, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI45439306, Length=292, Percent_Identity=26.3698630136986, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI5031815, Length=262, Percent_Identity=24.8091603053435, Blast_Score=83, Evalue=6e-16,
Organism=Homo sapiens, GI194272210, Length=262, Percent_Identity=24.8091603053435, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI226491502, Length=259, Percent_Identity=25.8687258687259, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=41.2859560067682, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1790571, Length=291, Percent_Identity=27.8350515463918, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1789256, Length=291, Percent_Identity=26.8041237113402, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=595, Percent_Identity=35.6302521008403, Blast_Score=308, Evalue=6e-84,
Organism=Caenorhabditis elegans, GI71994340, Length=250, Percent_Identity=26, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=250, Percent_Identity=26, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=251, Percent_Identity=26.6932270916335, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17551876, Length=273, Percent_Identity=27.1062271062271, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71984122, Length=298, Percent_Identity=25.8389261744966, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325153, Length=654, Percent_Identity=29.6636085626911, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6323011, Length=270, Percent_Identity=28.5185185185185, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=251, Percent_Identity=27.4900398406374, Blast_Score=77, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24584738, Length=622, Percent_Identity=33.6012861736334, Blast_Score=313, Evalue=3e-85,
Organism=Drosophila melanogaster, GI17136276, Length=261, Percent_Identity=27.9693486590038, Blast_Score=80, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24640851, Length=284, Percent_Identity=24.6478873239437, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640849, Length=284, Percent_Identity=24.6478873239437, Blast_Score=70, Evalue=6e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_ERYLH (Q2NBQ6)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457682.1
- ProteinModelPortal:   Q2NBQ6
- SMR:   Q2NBQ6
- STRING:   Q2NBQ6
- GeneID:   3869507
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_03965
- NMPDR:   fig|314225.3.peg.2284
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   YQLDVEM
- ProtClustDB:   PRK00476
- BioCyc:   ELIT314225:ELI_03965-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 67891; Mature: 67891

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYRTHNCAALSKKNVGETVRLSGWVHNKRDHGGVLFVDLRDHYGITQIVADEDSAALP
CCCCCCCCCHHHHHCCCCCEEEEECEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCC
VLDKLKLESVVTIDGDVKARDAEAVNPNLPTGEIEVFARSVEIQSRAEELPLIVNSAEDY
HHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHCCEEEECCCCC
PEDVRLKYRFVDLRRERLHRNIMLRSQVITSIRNRMTAQGFTEFQTPILAASSPEGARDY
CCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCCCCCCCCC
IVPSRLHPGTFYALPQAPQMFKQLLMVAGFDRYFQIAPCFRDEDLRADRSPEFYQLDFEM
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEEEEEEE
SFVTQEDVFQAIEPVLAGVFEEFANGKSVTKSGEFPRIPYAEAMLKYGTDKPDLRNPLVI
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEE
SDVTDHFQSSGFGLFEKIVGIGGRVRVIPAPNTQEKSRKFFDDMNDWARKEGFAGLGYVT
EHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
RKQGEFGGPIAKNHGPENMQKIYDELGLGENDGLFFAAGKEKDAAKLAGAARTRVGEELG
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LIEQGCFKFCWIVDFPMFEYDEELKKIDFSHNPFSMPQGEMEALENKDPLEILAWQYDIV
HHHHHHHHEEHEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEE
CNGYELSSGAIRNHKPEIMYKAFEIAGYDRETVDREFSGMIEAFKLGAPPHGGSAPGIDR
ECCEECCCCCCCCCCCHHHEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADEPNIREVIAFPLNQKAQDLMMGAPSQVPPKALRDVHIRTVEPPKKQQVGTHLV
EEEEEECCCCHHHHEECCCCCCHHHHHCCCCCCCCHHHHHCCEEEECCCCHHHHHCCCCC
NDDS
CCCC
>Mature Secondary Structure
MHAYRTHNCAALSKKNVGETVRLSGWVHNKRDHGGVLFVDLRDHYGITQIVADEDSAALP
CCCCCCCCCHHHHHCCCCCEEEEECEEECCCCCCCEEEEEECCCCCEEEEEECCCCCCCC
VLDKLKLESVVTIDGDVKARDAEAVNPNLPTGEIEVFARSVEIQSRAEELPLIVNSAEDY
HHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHEEEEHHHHHHHHHHHCCEEEECCCCC
PEDVRLKYRFVDLRRERLHRNIMLRSQVITSIRNRMTAQGFTEFQTPILAASSPEGARDY
CCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEECCCCCCCCCC
IVPSRLHPGTFYALPQAPQMFKQLLMVAGFDRYFQIAPCFRDEDLRADRSPEFYQLDFEM
CCCCCCCCCEEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCEEEEEEEE
SFVTQEDVFQAIEPVLAGVFEEFANGKSVTKSGEFPRIPYAEAMLKYGTDKPDLRNPLVI
EECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCEEE
SDVTDHFQSSGFGLFEKIVGIGGRVRVIPAPNTQEKSRKFFDDMNDWARKEGFAGLGYVT
EHHHHHHHCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEE
RKQGEFGGPIAKNHGPENMQKIYDELGLGENDGLFFAAGKEKDAAKLAGAARTRVGEELG
ECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LIEQGCFKFCWIVDFPMFEYDEELKKIDFSHNPFSMPQGEMEALENKDPLEILAWQYDIV
HHHHHHHHEEHEECCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCEEEEEEEEEE
CNGYELSSGAIRNHKPEIMYKAFEIAGYDRETVDREFSGMIEAFKLGAPPHGGSAPGIDR
ECCEECCCCCCCCCCCHHHEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
IVMLLADEPNIREVIAFPLNQKAQDLMMGAPSQVPPKALRDVHIRTVEPPKKQQVGTHLV
EEEEEECCCCHHHHEECCCCCCHHHHHCCCCCCCCHHHHHCCEEEECCCCHHHHHCCCCC
NDDS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA