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The map label for this gene is dapF

Identifier: 85373610

GI number: 85373610

Start: 807542

End: 808351

Strand: Reverse

Name: dapF

Synonym: ELI_03915

Alternate gene names: 85373610

Gene position: 808351-807542 (Counterclockwise)

Preceding gene: 85373611

Following gene: 85373609

Centisome position: 26.48

GC content: 66.67

Gene sequence:

>810_bases
ATGCGCGTCTCCTTCACCAAGATGCACGGCCTCGGCAATGATTTCATCATGCTCGACGCGCGTGCCGAGAGCCTGCCGCC
GATGACGGCTGCGACGGCGCGCGCGCTGGCGGATCGCAAGACCGGGATCGGCTGCGACCAGGTGATCCTGCTCGAACCTT
CCGACAGCGCAGACTTCCGCATGCGTATTTTCAACGCCGATGGCGGCGAAGTGGAAGCATGCGGCAATGCCTCGCGGGCG
GTCGCGCTGCTTCATGGCGAGGCGGCCAACGTCGAGACGAGCGGCGGCGTGATCGCGATAGCGCCCGCCAGTGGCGGCGC
GCGGGTCGACATGGGCGTGCCGCGCTTCGACTGGGAAGCGATCCCGCTGGCCTATGCCATGGACACATTGTCGCTGCCGG
TTGGCTGGGGGGAGCTGGAAGCGCCTGCCGCGGTCAATGTCGGCAATCCGCATGTGGTGTTCTTCGTGGATGATGCCGAT
GCGGTGCCGCTCGATACGATGGGACCCGGGATCGAGACCGATCCGCTGTTTCCCGAGCGTATCAACGTCAATATCGCCAG
CCTGACCGGCGACGATGCGCTCAAGCTGCACGTATGGGAACGCGGCGTCGGCCTGACCCGCGCTTGCGGCACCGGTGCCT
GCGCCACCGCCATCGCCGCAATGCGCCGCGGCCTGACCGGGCGCAAGGTCGCCGTGACCCTGCCCGGCGGCACGTTGCAG
ATCGAATGGGATGCAGACGACCACATCATCATGACCGGCCCCGCCGCCGAGAGCTTTCGCGGCACCTTCGACTGGGGCGA
CTATTCATGA

Upstream 100 bases:

>100_bases
TCCGACGAGGACGAGCCGCTGCAAAAGTCGGCCTGATCGCGGACGAATCGGCGGCGCGCGCTCTTTACGCGCGCTCCCGC
GACCCATAGATGCGCGGAAG

Downstream 100 bases:

>100_bases
GCGGAAGCGACGTCATTTCGCTCGGATGCCGCCTGAACATTTCCGAGAGCGCACAGATCCGCGCGCTGCTGGCGAGCGAA
GACGACGTGGTGGTCGTCAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MRVSFTKMHGLGNDFIMLDARAESLPPMTAATARALADRKTGIGCDQVILLEPSDSADFRMRIFNADGGEVEACGNASRA
VALLHGEAANVETSGGVIAIAPASGGARVDMGVPRFDWEAIPLAYAMDTLSLPVGWGELEAPAAVNVGNPHVVFFVDDAD
AVPLDTMGPGIETDPLFPERINVNIASLTGDDALKLHVWERGVGLTRACGTGACATAIAAMRRGLTGRKVAVTLPGGTLQ
IEWDADDHIIMTGPAAESFRGTFDWGDYS

Sequences:

>Translated_269_residues
MRVSFTKMHGLGNDFIMLDARAESLPPMTAATARALADRKTGIGCDQVILLEPSDSADFRMRIFNADGGEVEACGNASRA
VALLHGEAANVETSGGVIAIAPASGGARVDMGVPRFDWEAIPLAYAMDTLSLPVGWGELEAPAAVNVGNPHVVFFVDDAD
AVPLDTMGPGIETDPLFPERINVNIASLTGDDALKLHVWERGVGLTRACGTGACATAIAAMRRGLTGRKVAVTLPGGTLQ
IEWDADDHIIMTGPAAESFRGTFDWGDYS
>Mature_269_residues
MRVSFTKMHGLGNDFIMLDARAESLPPMTAATARALADRKTGIGCDQVILLEPSDSADFRMRIFNADGGEVEACGNASRA
VALLHGEAANVETSGGVIAIAPASGGARVDMGVPRFDWEAIPLAYAMDTLSLPVGWGELEAPAAVNVGNPHVVFFVDDAD
AVPLDTMGPGIETDPLFPERINVNIASLTGDDALKLHVWERGVGLTRACGTGACATAIAAMRRGLTGRKVAVTLPGGTLQ
IEWDADDHIIMTGPAAESFRGTFDWGDYS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=277, Percent_Identity=37.9061371841155, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ERYLH (Q2NBR6)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457672.1
- HSSP:   P44859
- ProteinModelPortal:   Q2NBR6
- SMR:   Q2NBR6
- STRING:   Q2NBR6
- GeneID:   3870550
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_03915
- NMPDR:   fig|314225.3.peg.2294
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q2NBR6
- ProtClustDB:   PRK00450
- BioCyc:   ELIT314225:ELI_03915-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28238; Mature: 28238

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 74-74 ACT_SITE 209-209

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSFTKMHGLGNDFIMLDARAESLPPMTAATARALADRKTGIGCDQVILLEPSDSADFR
CCEEEEHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEE
MRIFNADGGEVEACGNASRAVALLHGEAANVETSGGVIAIAPASGGARVDMGVPRFDWEA
EEEEECCCCEEEECCCCCEEEEEEECCCCCEECCCCEEEEEECCCCCEEECCCCCCCCCC
IPLAYAMDTLSLPVGWGELEAPAAVNVGNPHVVFFVDDADAVPLDTMGPGIETDPLFPER
CCCEEEEEEEECCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
INVNIASLTGDDALKLHVWERGVGLTRACGTGACATAIAAMRRGLTGRKVAVTLPGGTLQ
EEEEEEEECCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEE
IEWDADDHIIMTGPAAESFRGTFDWGDYS
EEECCCCCEEEECCCCHHCCCCCCCCCCC
>Mature Secondary Structure
MRVSFTKMHGLGNDFIMLDARAESLPPMTAATARALADRKTGIGCDQVILLEPSDSADFR
CCEEEEHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEE
MRIFNADGGEVEACGNASRAVALLHGEAANVETSGGVIAIAPASGGARVDMGVPRFDWEA
EEEEECCCCEEEECCCCCEEEEEEECCCCCEECCCCEEEEEECCCCCEEECCCCCCCCCC
IPLAYAMDTLSLPVGWGELEAPAAVNVGNPHVVFFVDDADAVPLDTMGPGIETDPLFPER
CCCEEEEEEEECCCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
INVNIASLTGDDALKLHVWERGVGLTRACGTGACATAIAAMRRGLTGRKVAVTLPGGTLQ
EEEEEEEECCCCEEEEEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEE
IEWDADDHIIMTGPAAESFRGTFDWGDYS
EEECCCCCEEEECCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA