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The map label for this gene is pnp

Identifier: 85373534

GI number: 85373534

Start: 730555

End: 732864

Strand: Direct

Name: pnp

Synonym: ELI_03535

Alternate gene names: 85373534

Gene position: 730555-732864 (Clockwise)

Preceding gene: 85373533

Following gene: 85373535

Centisome position: 23.93

GC content: 62.47

Gene sequence:

>2310_bases
ATGTTCGACAAGAAAACCGTATCGATTGAATGGGGCGGAAAAACCCTCACTCTCGAAACCGGCCAGATTGCCCGTCAGGC
TGACGGTGCCGTGCTGGCCACCTATGGCGAAACCGTGGTGCTGTGCGCCGTGACCGCCGCCCGGAGCGTCAAGGAAGGGC
AGGACTTCTTCCCGCTGACCGTCCACTACCAGGAAAAATTCTCCGCTGCTGGCCGTATCCCCGGCGGCTTCTTCAAGCGC
GAAGGCCGCGCGACGGAGAAGGAAACGCTGACCTCGCGCCTGATCGATCGCCCCGTGCGGCCGCTGTTCCCGGAAGGGTT
CTACAACGAAATCAACGTGATCGCGCAGGTCCTCAGCTATGACGGCGAAACCGAGCCTGACATTGTCGCGATGATTGCCG
CTTCGGCTGCGCTGACGATCTCCGGCCTGCCTTTCATGGGCCCGATCGGCGCCGCGCGCGTCGGCTTCACCAATGACGGC
GAATACGTCCTCAACCCGTCCGTCGTCGACGCGCTGGGTGAAGACGGCAATCTCGATCTCGTCGTCGCTGCAACCCAAGA
CGCGGTGATGATGGTGGAATCCGAAGCCAAGGAACTGACCGAAGAGCAGATGCTCGGCGCCGTCATGTTCGCGCACGAGG
AAAGCCGCAAGGTCATCGGTGCGATCATCGATCTGGCCGAACAGGCTGCCAAGGATCCGTGGGAACTCGATCCGGTCGAA
GACAAGTCCGCCGCTCTGGAAAAGCTGCGCGGTGTCATCGGCGACGATCTCGCCAAGGCCTACAAGATCACCAACAAGGC
TCAGCGTCAGGACGCGGTAAATGCCGCTCGCACCAAGGCGCGCGAGCACTATTCCGATCTCGAAGAGAGCGATCCGGCTG
AATACATGGGCCGCCTCAAGCTCGTGAAGAAGCTGGAAAGCGACATCGTGCGCAAGGCGATCCTCAAGGATGGCCAGCGT
ATCGACGGCCGCAAGACCGACGAAGTCCGCCCGATCGAAGCGATGGTCGGCCTCCTGCCGCGGACGCACGGTTCGGCGCT
GTTCACGCGCGGTGAAACGCAGGCAATCTGCACCACCACGCTGGGCACGAAGGACTCCGAGCAGATGATCGACGGGCTGG
AAGGCCTTAGCTACAGCAATTTCATGTTGCACTATAACTTCCCGCCCTATTCGGTCGGGGAAGTGGGCCGTTTCGGCTTC
ACCAGCCGCCGCGAGACCGGCCATGGCAAGCTTGCCTTCCGTGCGCTGCGTCCGGTCCTGCCCGATAACGAGGAATTCCC
CTACACCATCCGCGTTCTGTCCGACATCACCGAGTCCAACGGCTCGAGCTCGATGGCGACGGTATGTGGTGGCTCGCTCT
CGATGATGGATGCCGGCGTTCCGCTCAAGCGTCCGGTTTCGGGTATTGCGATGGGCCTGATCCTCGAAGGCGATGACTTC
ACCGTCCTCTCCGACATCCTGGGCGATGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCTGGTTCGGAAGAAGGCAT
CACCAGCCTCCAGATGGATATCAAGGTGGCCGGCATCACGCAGGAAATCATGACCAAGGCGCTCGAACAGGCGAAGGCCG
GTCGTGCGCACATCCTGGGCAAGATGACCGAAGCCCTCGGGTCCTCGCGCGGCGAAGTCTCGAAGCACGCTCCGCGTATC
GAGACGATGCAGATCGACAAGTCGAAGATCCGCGATGTCATCGGCACGGGCGGCAAGGTGATCCGCGAGATCGTCGCCGA
AACCGGCGCCAAGGTCGACATCGACGACGAGGGCGTGATCAAGATCAGCTCTTCCAACGCCGACGAGATCGAAGCGGCAC
GCAAGTGGATCGAAGGCATCGTCGAAGAGGCGGAAGTCGGCAAGATCTACAACGGCAAGGTCGTCAACATCGTCGACTTC
GGTGCATTCGTGAACTTCATGGGCGGCAAGGACGGTCTCGTCCACGTCAGCGAAATGAAGAACGAGCGCGTCGAGAAGCC
GACCGATGTCGTGTCGGAAGGCCAGGAAGTGAAGGTCAAGGTCCTCGAGATCGACCAGCGCGGCAAGGTTCGCCTGTCGA
TGCGCGTGGTCGACCAGGAAACCGGCGAAGAGCTGGAAGACACCCGCCCGCCACGCGAACCGCGCGGTGATCGTGGCCCG
CGTAGCGGCGGTGGCGATCGTCGTGGTGGCCGTGGTGGCCCGCGTCGTGGCGGTGGTGGCGGTGGCGGCGGTGGCCGCGA
TCGCGGCGGTCGTGACGGCGGCAACGACGGCGGCGGCGAAGCCCATGTGCCGGACTTCCTGAAGGACTGA

Upstream 100 bases:

>100_bases
CAAGGGGCACACAAAAGCCCCATACCGCACCGGGGCGGCTACCCCGGAACAGTAGGCCCCGCATCGCAATAAGGCCGTGC
GGGTCATTTTAGGAAAACAC

Downstream 100 bases:

>100_bases
CGTTTCGTGTCGCCCCAGCGCAAGCTGGGGCCTCCGTCCGCCTGGTCGGATCCTGCCGCACCAGATCCCGGCTTTCGCTG
GGATGATGGAGAAAGAGAGA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MFDKKTVSIEWGGKTLTLETGQIARQADGAVLATYGETVVLCAVTAARSVKEGQDFFPLTVHYQEKFSAAGRIPGGFFKR
EGRATEKETLTSRLIDRPVRPLFPEGFYNEINVIAQVLSYDGETEPDIVAMIAASAALTISGLPFMGPIGAARVGFTNDG
EYVLNPSVVDALGEDGNLDLVVAATQDAVMMVESEAKELTEEQMLGAVMFAHEESRKVIGAIIDLAEQAAKDPWELDPVE
DKSAALEKLRGVIGDDLAKAYKITNKAQRQDAVNAARTKAREHYSDLEESDPAEYMGRLKLVKKLESDIVRKAILKDGQR
IDGRKTDEVRPIEAMVGLLPRTHGSALFTRGETQAICTTTLGTKDSEQMIDGLEGLSYSNFMLHYNFPPYSVGEVGRFGF
TSRRETGHGKLAFRALRPVLPDNEEFPYTIRVLSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILEGDDF
TVLSDILGDEDHLGDMDFKVAGSEEGITSLQMDIKVAGITQEIMTKALEQAKAGRAHILGKMTEALGSSRGEVSKHAPRI
ETMQIDKSKIRDVIGTGGKVIREIVAETGAKVDIDDEGVIKISSSNADEIEAARKWIEGIVEEAEVGKIYNGKVVNIVDF
GAFVNFMGGKDGLVHVSEMKNERVEKPTDVVSEGQEVKVKVLEIDQRGKVRLSMRVVDQETGEELEDTRPPREPRGDRGP
RSGGGDRRGGRGGPRRGGGGGGGGGRDRGGRDGGNDGGGEAHVPDFLKD

Sequences:

>Translated_769_residues
MFDKKTVSIEWGGKTLTLETGQIARQADGAVLATYGETVVLCAVTAARSVKEGQDFFPLTVHYQEKFSAAGRIPGGFFKR
EGRATEKETLTSRLIDRPVRPLFPEGFYNEINVIAQVLSYDGETEPDIVAMIAASAALTISGLPFMGPIGAARVGFTNDG
EYVLNPSVVDALGEDGNLDLVVAATQDAVMMVESEAKELTEEQMLGAVMFAHEESRKVIGAIIDLAEQAAKDPWELDPVE
DKSAALEKLRGVIGDDLAKAYKITNKAQRQDAVNAARTKAREHYSDLEESDPAEYMGRLKLVKKLESDIVRKAILKDGQR
IDGRKTDEVRPIEAMVGLLPRTHGSALFTRGETQAICTTTLGTKDSEQMIDGLEGLSYSNFMLHYNFPPYSVGEVGRFGF
TSRRETGHGKLAFRALRPVLPDNEEFPYTIRVLSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILEGDDF
TVLSDILGDEDHLGDMDFKVAGSEEGITSLQMDIKVAGITQEIMTKALEQAKAGRAHILGKMTEALGSSRGEVSKHAPRI
ETMQIDKSKIRDVIGTGGKVIREIVAETGAKVDIDDEGVIKISSSNADEIEAARKWIEGIVEEAEVGKIYNGKVVNIVDF
GAFVNFMGGKDGLVHVSEMKNERVEKPTDVVSEGQEVKVKVLEIDQRGKVRLSMRVVDQETGEELEDTRPPREPRGDRGP
RSGGGDRRGGRGGPRRGGGGGGGGGRDRGGRDGGNDGGGEAHVPDFLKD
>Mature_769_residues
MFDKKTVSIEWGGKTLTLETGQIARQADGAVLATYGETVVLCAVTAARSVKEGQDFFPLTVHYQEKFSAAGRIPGGFFKR
EGRATEKETLTSRLIDRPVRPLFPEGFYNEINVIAQVLSYDGETEPDIVAMIAASAALTISGLPFMGPIGAARVGFTNDG
EYVLNPSVVDALGEDGNLDLVVAATQDAVMMVESEAKELTEEQMLGAVMFAHEESRKVIGAIIDLAEQAAKDPWELDPVE
DKSAALEKLRGVIGDDLAKAYKITNKAQRQDAVNAARTKAREHYSDLEESDPAEYMGRLKLVKKLESDIVRKAILKDGQR
IDGRKTDEVRPIEAMVGLLPRTHGSALFTRGETQAICTTTLGTKDSEQMIDGLEGLSYSNFMLHYNFPPYSVGEVGRFGF
TSRRETGHGKLAFRALRPVLPDNEEFPYTIRVLSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILEGDDF
TVLSDILGDEDHLGDMDFKVAGSEEGITSLQMDIKVAGITQEIMTKALEQAKAGRAHILGKMTEALGSSRGEVSKHAPRI
ETMQIDKSKIRDVIGTGGKVIREIVAETGAKVDIDDEGVIKISSSNADEIEAARKWIEGIVEEAEVGKIYNGKVVNIVDF
GAFVNFMGGKDGLVHVSEMKNERVEKPTDVVSEGQEVKVKVLEIDQRGKVRLSMRVVDQETGEELEDTRPPREPRGDRGP
RSGGGDRRGGRGGPRRGGGGGGGGGRDRGGRDGGNDGGGEAHVPDFLKD

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=724, Percent_Identity=36.4640883977901, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI4826690, Length=92, Percent_Identity=53.2608695652174, Blast_Score=83, Evalue=9e-16,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=54.1007194244604, Blast_Score=747, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=109, Percent_Identity=42.2018348623853, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115534063, Length=675, Percent_Identity=33.037037037037, Blast_Score=344, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI17535281, Length=140, Percent_Identity=40.7142857142857, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320850, Length=107, Percent_Identity=42.0560747663551, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI281362905, Length=712, Percent_Identity=36.376404494382, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651641, Length=712, Percent_Identity=36.376404494382, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI24651643, Length=712, Percent_Identity=36.376404494382, Blast_Score=441, Evalue=1e-123,
Organism=Drosophila melanogaster, GI161079377, Length=655, Percent_Identity=36.4885496183206, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI20129977, Length=91, Percent_Identity=47.2527472527472, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_ERYLH (Q2NBZ2)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457596.1
- ProteinModelPortal:   Q2NBZ2
- SMR:   Q2NBZ2
- STRING:   Q2NBZ2
- GeneID:   3870494
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_03535
- NMPDR:   fig|314225.3.peg.2374
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q2NBZ2
- ProtClustDB:   PRK11824
- BioCyc:   ELIT314225:ELI_03535-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 82951; Mature: 82951

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKKTVSIEWGGKTLTLETGQIARQADGAVLATYGETVVLCAVTAARSVKEGQDFFPLT
CCCCEEEEEEECCEEEEEECCHHHHHCCCCEEEECCCEEEEEHHHHHHHHHCCCCCCEEE
VHYQEKFSAAGRIPGGFFKREGRATEKETLTSRLIDRPVRPLFPEGFYNEINVIAQVLSY
EEEHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
DGETEPDIVAMIAASAALTISGLPFMGPIGAARVGFTNDGEYVLNPSVVDALGEDGNLDL
CCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCEECCCCCCCEEECCHHHHHCCCCCCEEE
VVAATQDAVMMVESEAKELTEEQMLGAVMFAHEESRKVIGAIIDLAEQAAKDPWELDPVE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DKSAALEKLRGVIGDDLAKAYKITNKAQRQDAVNAARTKAREHYSDLEESDPAEYMGRLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVKKLESDIVRKAILKDGQRIDGRKTDEVRPIEAMVGLLPRTHGSALFTRGETQAICTTT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEE
LGTKDSEQMIDGLEGLSYSNFMLHYNFPPYSVGEVGRFGFTSRRETGHGKLAFRALRPVL
CCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
PDNEEFPYTIRVLSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILEGDDF
CCCCCCCEEEHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCC
TVLSDILGDEDHLGDMDFKVAGSEEGITSLQMDIKVAGITQEIMTKALEQAKAGRAHILG
HHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHH
KMTEALGSSRGEVSKHAPRIETMQIDKSKIRDVIGTGGKVIREIVAETGAKVDIDDEGVI
HHHHHHCCCCCCHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEE
KISSSNADEIEAARKWIEGIVEEAEVGKIYNGKVVNIVDFGAFVNFMGGKDGLVHVSEMK
EECCCCHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEHHHHHHHCCCCCCEEEHHHHH
NERVEKPTDVVSEGQEVKVKVLEIDQRGKVRLSMRVVDQETGEELEDTRPPREPRGDRGP
HHHCCCCHHHHCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCC
RSGGGDRRGGRGGPRRGGGGGGGGGRDRGGRDGGNDGGGEAHVPDFLKD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MFDKKTVSIEWGGKTLTLETGQIARQADGAVLATYGETVVLCAVTAARSVKEGQDFFPLT
CCCCEEEEEEECCEEEEEECCHHHHHCCCCEEEECCCEEEEEHHHHHHHHHCCCCCCEEE
VHYQEKFSAAGRIPGGFFKREGRATEKETLTSRLIDRPVRPLFPEGFYNEINVIAQVLSY
EEEHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
DGETEPDIVAMIAASAALTISGLPFMGPIGAARVGFTNDGEYVLNPSVVDALGEDGNLDL
CCCCCCHHHHHHHHHHHHEECCCCCCCCCCCCEECCCCCCCEEECCHHHHHCCCCCCEEE
VVAATQDAVMMVESEAKELTEEQMLGAVMFAHEESRKVIGAIIDLAEQAAKDPWELDPVE
EEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DKSAALEKLRGVIGDDLAKAYKITNKAQRQDAVNAARTKAREHYSDLEESDPAEYMGRLK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LVKKLESDIVRKAILKDGQRIDGRKTDEVRPIEAMVGLLPRTHGSALFTRGETQAICTTT
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEEEE
LGTKDSEQMIDGLEGLSYSNFMLHYNFPPYSVGEVGRFGFTSRRETGHGKLAFRALRPVL
CCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
PDNEEFPYTIRVLSDITESNGSSSMATVCGGSLSMMDAGVPLKRPVSGIAMGLILEGDDF
CCCCCCCEEEHHHHHHHHCCCCCCCHHHCCCCHHHHHCCCCCCCCCCCEEEEEEEECCCC
TVLSDILGDEDHLGDMDFKVAGSEEGITSLQMDIKVAGITQEIMTKALEQAKAGRAHILG
HHHHHHCCCCCCCCCCCEEECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHCCHHHHHH
KMTEALGSSRGEVSKHAPRIETMQIDKSKIRDVIGTGGKVIREIVAETGAKVDIDDEGVI
HHHHHHCCCCCCHHHCCCCCEEEECCHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCEE
KISSSNADEIEAARKWIEGIVEEAEVGKIYNGKVVNIVDFGAFVNFMGGKDGLVHVSEMK
EECCCCHHHHHHHHHHHHHHHHHHHCCEEECCCEEEEEEHHHHHHHCCCCCCEEEHHHHH
NERVEKPTDVVSEGQEVKVKVLEIDQRGKVRLSMRVVDQETGEELEDTRPPREPRGDRGP
HHHCCCCHHHHCCCCEEEEEEEEECCCCCEEEEEEEECCCCCCHHHHCCCCCCCCCCCCC
RSGGGDRRGGRGGPRRGGGGGGGGGRDRGGRDGGNDGGGEAHVPDFLKD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA