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The map label for this gene is leuC [H]

Identifier: 85373489

GI number: 85373489

Start: 697401

End: 698864

Strand: Reverse

Name: leuC [H]

Synonym: ELI_03310

Alternate gene names: 85373489

Gene position: 698864-697401 (Counterclockwise)

Preceding gene: 85373493

Following gene: 85373488

Centisome position: 22.9

GC content: 63.66

Gene sequence:

>1464_bases
GTGTTGCGCAAAGGTCGCAAAGCTGCGGCCATGGAGAGCACCCCGCGCACATTGTACGAGAAAATCTGGGACGCGCATGT
TGTCGAACAGCGCGACGATGGCACCGCGCTGATCTATATCGACCGGCATCTCGTCCATGAGGTTACCAGTCCGCAGGCGT
TCGAGGCTTTGCGGGTCAGCGGCCGTACCGTGCGCCGTCCCGACCTGACGCTGGCGGTGCCCGACCACAACCTGCCGACC
ACAGCACGGCGTACGTCGTCCGGTTCGCGCATCCCGATCGCCGACCCGCAAAGCGCGGCGCAATTGGACGCTCTCGCCCG
AAATGCGGCCGAATTCGGCATCCGCTATATCGGTGATGCCGATGCCGAGCAGGGAATTGTTCACGTGGTGGGGCCCGAGC
AAGGCTTCTCGCTCCCCGGCGCGACGATCGTATGCGGGGACTCGCACACCGCCTGTCACGGTGGTTTGGGTGCCCTCGCC
TTCGGCATCGGCACCAGCGAAGTCGAGCACGTGCTGGCAACGCAGACGCTGCTGCTCAAGCAGTCGAAAACGATGGAAAT
CCGTGTTGAAGGCGAACTGGGCGCGGGTGTCACCTCGAAAGACCTGATCATGCACATCATCGGCGTGATCGGAGCAGCGG
GCGGGACCGGATATGTCTTCGAATATCGCGGCAAAGTGTTCGAGCGCATGAGCATCGAAAGCCGCCTTACGGTCTGCAAC
ATGAGCATCGAAGCCGGTGCCCGCGCAGGCCTCATCGCACCGGACGAGACGACCTTCGCCTATTTGAAAGGCCGCCCCTA
TGCGCCCGACGGCGAAGAGTGGGATGCAGCAGTCGCCTGGTGGCGCTCGCTCGCAACCGATGACGGCGCGACATTCGACA
GATCGGTCACGATCGATGCGGCGGAGGTCGCCCCGAGCGTCACCTGGGGCACCAGCCCCGAAGATGTCCTGCCGATCACT
GGCGAAGTGCCATCCCCCGCAAGTTTCGACGACACCGGCAAACAGGCGGCCGCTGCCAAGAGCCTCGAATACATGGGCCT
CACCCCCGGCCAGAAATTGACGGAGATTTCGGTCGAGAACATCTTCATCGGCAGTTGCACAAACAGCCGCATCGAAGACC
TGCGCGCTGCCGCCAAGGTGCTGCGAGGACGGAGAAAAGCGAGCAACGTAAACTGGGCCATCGTTGTCCCGGGCTCAGGC
CTCGTGAAGCGTCAGGCTGAAGAGGAAGGCCTCGACCGCATTTTTACCGAGGCCGGCTTCGAATGGCGCGAACCCGGCTG
TTCGGCCTGCCTCGGCATGAACCCCGACAAGGTCCCGCCGGGCCAGCGCTGCGCATCGACGAGCAATCGCAATTTCGTCG
GTCGCCAGGGCCCGGGTGCGCGAACGCACCTTTTGTCGCCTGCCATGGCCGCTGCGGCGGCCGTCAACGGTCGGCTCACC
GATGTTCGGGAGTTGTCCGACTAA

Upstream 100 bases:

>100_bases
AATGGCTTGGCGGGGGAGCTTGCTGACATGACTGTCAGTTAGGCGCGGAAAGCTTTCTTTTCCATGACACCGCAATTGCT
TGGGCCACGTAATCTTGCCT

Downstream 100 bases:

>100_bases
TCGCCCTTGCGCGGCGCGCAACACATCACCAAAGCAGAGAGCATGACGAAAAAACTTCACGCCAAGACCATCGCCGGAGC
CATCGGATCCGCCGCTATCG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MLRKGRKAAAMESTPRTLYEKIWDAHVVEQRDDGTALIYIDRHLVHEVTSPQAFEALRVSGRTVRRPDLTLAVPDHNLPT
TARRTSSGSRIPIADPQSAAQLDALARNAAEFGIRYIGDADAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALA
FGIGTSEVEHVLATQTLLLKQSKTMEIRVEGELGAGVTSKDLIMHIIGVIGAAGGTGYVFEYRGKVFERMSIESRLTVCN
MSIEAGARAGLIAPDETTFAYLKGRPYAPDGEEWDAAVAWWRSLATDDGATFDRSVTIDAAEVAPSVTWGTSPEDVLPIT
GEVPSPASFDDTGKQAAAAKSLEYMGLTPGQKLTEISVENIFIGSCTNSRIEDLRAAAKVLRGRRKASNVNWAIVVPGSG
LVKRQAEEEGLDRIFTEAGFEWREPGCSACLGMNPDKVPPGQRCASTSNRNFVGRQGPGARTHLLSPAMAAAAAVNGRLT
DVRELSD

Sequences:

>Translated_487_residues
MLRKGRKAAAMESTPRTLYEKIWDAHVVEQRDDGTALIYIDRHLVHEVTSPQAFEALRVSGRTVRRPDLTLAVPDHNLPT
TARRTSSGSRIPIADPQSAAQLDALARNAAEFGIRYIGDADAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALA
FGIGTSEVEHVLATQTLLLKQSKTMEIRVEGELGAGVTSKDLIMHIIGVIGAAGGTGYVFEYRGKVFERMSIESRLTVCN
MSIEAGARAGLIAPDETTFAYLKGRPYAPDGEEWDAAVAWWRSLATDDGATFDRSVTIDAAEVAPSVTWGTSPEDVLPIT
GEVPSPASFDDTGKQAAAAKSLEYMGLTPGQKLTEISVENIFIGSCTNSRIEDLRAAAKVLRGRRKASNVNWAIVVPGSG
LVKRQAEEEGLDRIFTEAGFEWREPGCSACLGMNPDKVPPGQRCASTSNRNFVGRQGPGARTHLLSPAMAAAAAVNGRLT
DVRELSD
>Mature_487_residues
MLRKGRKAAAMESTPRTLYEKIWDAHVVEQRDDGTALIYIDRHLVHEVTSPQAFEALRVSGRTVRRPDLTLAVPDHNLPT
TARRTSSGSRIPIADPQSAAQLDALARNAAEFGIRYIGDADAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALA
FGIGTSEVEHVLATQTLLLKQSKTMEIRVEGELGAGVTSKDLIMHIIGVIGAAGGTGYVFEYRGKVFERMSIESRLTVCN
MSIEAGARAGLIAPDETTFAYLKGRPYAPDGEEWDAAVAWWRSLATDDGATFDRSVTIDAAEVAPSVTWGTSPEDVLPIT
GEVPSPASFDDTGKQAAAAKSLEYMGLTPGQKLTEISVENIFIGSCTNSRIEDLRAAAKVLRGRRKASNVNWAIVVPGSG
LVKRQAEEEGLDRIFTEAGFEWREPGCSACLGMNPDKVPPGQRCASTSNRNFVGRQGPGARTHLLSPAMAAAAAVNGRLT
DVRELSD

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=389, Percent_Identity=26.7352185089974, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI8659555, Length=398, Percent_Identity=27.1356783919598, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=26.8421052631579, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786259, Length=471, Percent_Identity=58.8110403397028, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=25.9668508287293, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI2367097, Length=354, Percent_Identity=25.1412429378531, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=395, Percent_Identity=28.6075949367089, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=395, Percent_Identity=28.6075949367089, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI25149342, Length=326, Percent_Identity=28.5276073619632, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=402, Percent_Identity=27.6119402985075, Blast_Score=103, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=61.1464968152866, Blast_Score=608, Evalue=1e-175,
Organism=Saccharomyces cerevisiae, GI6322261, Length=386, Percent_Identity=29.2746113989637, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323335, Length=365, Percent_Identity=29.041095890411, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320440, Length=435, Percent_Identity=23.2183908045977, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281365315, Length=398, Percent_Identity=27.1356783919598, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864292, Length=398, Percent_Identity=27.1356783919598, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI161076999, Length=398, Percent_Identity=27.3869346733668, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571643, Length=373, Percent_Identity=27.6139410187668, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24645686, Length=373, Percent_Identity=29.2225201072386, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17137564, Length=368, Percent_Identity=27.9891304347826, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 51990; Mature: 51990

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKGRKAAAMESTPRTLYEKIWDAHVVEQRDDGTALIYIDRHLVHEVTSPQAFEALRVS
CCCCCCCHHHHCCCHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHC
GRTVRRPDLTLAVPDHNLPTTARRTSSGSRIPIADPQSAAQLDALARNAAEFGIRYIGDA
CCEEECCCEEEEECCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCC
DAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVEHVLATQTLLLK
CCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHC
QSKTMEIRVEGELGAGVTSKDLIMHIIGVIGAAGGTGYVFEYRGKVFERMSIESRLTVCN
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHEEEEEE
MSIEAGARAGLIAPDETTFAYLKGRPYAPDGEEWDAAVAWWRSLATDDGATFDRSVTIDA
EEHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEH
AEVAPSVTWGTSPEDVLPITGEVPSPASFDDTGKQAAAAKSLEYMGLTPGQKLTEISVEN
HHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCCEEEEEECE
IFIGSCTNSRIEDLRAAAKVLRGRRKASNVNWAIVVPGSGLVKRQAEEEGLDRIFTEAGF
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCC
EWREPGCSACLGMNPDKVPPGQRCASTSNRNFVGRQGPGARTHLLSPAMAAAAAVNGRLT
CCCCCCCCEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHCCCCH
DVRELSD
HHHHCCC
>Mature Secondary Structure
MLRKGRKAAAMESTPRTLYEKIWDAHVVEQRDDGTALIYIDRHLVHEVTSPQAFEALRVS
CCCCCCCHHHHCCCHHHHHHHHHHHHHHEECCCCCEEEEEEHHHHHHCCCCHHHHHHHHC
GRTVRRPDLTLAVPDHNLPTTARRTSSGSRIPIADPQSAAQLDALARNAAEFGIRYIGDA
CCEEECCCEEEEECCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCC
DAEQGIVHVVGPEQGFSLPGATIVCGDSHTACHGGLGALAFGIGTSEVEHVLATQTLLLK
CCCCCEEEEECCCCCCCCCCCEEEECCCCCCHHCCHHHHHCCCCHHHHHHHHHHHHHHHC
QSKTMEIRVEGELGAGVTSKDLIMHIIGVIGAAGGTGYVFEYRGKVFERMSIESRLTVCN
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHEEEEEE
MSIEAGARAGLIAPDETTFAYLKGRPYAPDGEEWDAAVAWWRSLATDDGATFDRSVTIDA
EEHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEH
AEVAPSVTWGTSPEDVLPITGEVPSPASFDDTGKQAAAAKSLEYMGLTPGQKLTEISVEN
HHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCCEEEEEECE
IFIGSCTNSRIEDLRAAAKVLRGRRKASNVNWAIVVPGSGLVKRQAEEEGLDRIFTEAGF
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCC
EWREPGCSACLGMNPDKVPPGQRCASTSNRNFVGRQGPGARTHLLSPAMAAAAAVNGRLT
CCCCCCCCEECCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHCCCCH
DVRELSD
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA