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The map label for this gene is 85373410

Identifier: 85373410

GI number: 85373410

Start: 614707

End: 616077

Strand: Reverse

Name: 85373410

Synonym: ELI_02915

Alternate gene names: NA

Gene position: 616077-614707 (Counterclockwise)

Preceding gene: 228879506

Following gene: 85373409

Centisome position: 20.18

GC content: 63.68

Gene sequence:

>1371_bases
ATGAGCACGCGGGAGGATAGCGGGGCCGACGAGCCGATCATCGAATCGCGCGACCAGTTGGTGGCGCCGATGCAGGCGGG
CGAGAAACCGAAAGACCGCTGGCGCATCGGTACCGAACACGAGAAGCTCGTCTACAAACGCGACGGATTCCGCGCGCCTT
CCTACGACGAAGACGGAGGTATCCGCGATATCCTGCTGGAAATGCAGCAGTTCGGCTGGCAGCCGGTCGAGGAGGATGGA
AAGGTCATCGCACTCAAGGGCGAGGACGGGGCGGTCAGTCTCGAGCCTGCCGGGCAGCTCGAGCTCAGCGGCGCGCCGCT
CGAAAACCTGCACCAGACCTGCAACGAAACCGGCCGCCACCTGACGCAGGTCAAGGCGATCGGCGAGAAATGCGGCGTCG
GCTTCCTCGGCCTCGGCATGTGGCCCGACAAGACCCGCGAAGAGCTGCCGGTCATGCCCAAGGGACGCTATGACATCATG
ATGCGGCACATGCCGCGCGTCGGCAGCCTCGGTCTCGACATGATGCTGCGCACCTGCACCATCCAGGTCAATCTCGACTA
TTCCAGCGAAGCCGATATGGCGAAGAAATTTCGCACGAGCCTCGCACTGCAACCGCTCGCCACCGCGCTGTTCGCCAACT
CTCCTTTCACCGAGGGCAAGCCCAACGACTTCCTCAGCTATCGCAGCCATATCTGGTCGGACACCGATCCGCATCGCACC
GGCATGCTGCCTTTCGTGTTCGAGGATGGCTTCGGCTATGAGCGCTGGACCGACTACATGCTCGATGTGCCGATGTATTT
CGTCTATCGCGACGGCAAATATATCGACGCAGCGGGCCACAGCTTCCGCGATTTTCTGGACGGGAAGCTGTCCGTCCTTC
CCGGCGAAAAACCGCGGCCAAGCGACTGGTGGGATCACCTCAGCACCGCCTTCCCCGAAGTGCGCCTCAAAAGCTTTCTC
GAAATGCGCGGCGCAGACGGCGGGCCCTGGAGCCGCATCTGCGCCCTGCCTGCCCTCTGGGTCGGCCTGCTCTACGACGA
TTCCGCGCTCGACGCCGCTTGGGACCTCGTCAAGGACTGGTCGATGGAAGAGCGCGAAGCGCTGCGTAATGCGGTGCCCA
GGCTCGCGCTCGACGCACCCATCCCGGGAGGGCATCGCTTGCAGGACCTCGCCCGAGACGTGCTGAAAATCGCCCGCCAG
GGCCTCACCGCCCGCGCCCGCCTCGGCGAAAGCGGCGACAACGAAACCGGCTTCCTCTCGACGCTCGACGAGATCGTCGA
GACCGGCAAGGTCCCGGCGCAGCGCCTGCTCGACATGTATCACGGCGAATGGGGCGGCGATATCAAGCGGGTCTACAAAT
ACAGTTTCTGA

Upstream 100 bases:

>100_bases
TAGTCGCAGTGGGGTGGGCTGTCCGGCAAAAGATTAGGCGTTGCATCGGAAAACCGATTTTTCTTGGCCTCGGCAAATCC
GCCGCCTAACGCGCTGAATT

Downstream 100 bases:

>100_bases
CGCACGCACAGCCGATCGAGGGAGAGGACACATGATCCAGATCAACGGCACGATTCAACTGGCCCCCGGCACGGTTGCCG
ACAATCCGGACACGGTCGAA

Product: glutamate-cysteine ligase precursor

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSTREDSGADEPIIESRDQLVAPMQAGEKPKDRWRIGTEHEKLVYKRDGFRAPSYDEDGGIRDILLEMQQFGWQPVEEDG
KVIALKGEDGAVSLEPAGQLELSGAPLENLHQTCNETGRHLTQVKAIGEKCGVGFLGLGMWPDKTREELPVMPKGRYDIM
MRHMPRVGSLGLDMMLRTCTIQVNLDYSSEADMAKKFRTSLALQPLATALFANSPFTEGKPNDFLSYRSHIWSDTDPHRT
GMLPFVFEDGFGYERWTDYMLDVPMYFVYRDGKYIDAAGHSFRDFLDGKLSVLPGEKPRPSDWWDHLSTAFPEVRLKSFL
EMRGADGGPWSRICALPALWVGLLYDDSALDAAWDLVKDWSMEEREALRNAVPRLALDAPIPGGHRLQDLARDVLKIARQ
GLTARARLGESGDNETGFLSTLDEIVETGKVPAQRLLDMYHGEWGGDIKRVYKYSF

Sequences:

>Translated_456_residues
MSTREDSGADEPIIESRDQLVAPMQAGEKPKDRWRIGTEHEKLVYKRDGFRAPSYDEDGGIRDILLEMQQFGWQPVEEDG
KVIALKGEDGAVSLEPAGQLELSGAPLENLHQTCNETGRHLTQVKAIGEKCGVGFLGLGMWPDKTREELPVMPKGRYDIM
MRHMPRVGSLGLDMMLRTCTIQVNLDYSSEADMAKKFRTSLALQPLATALFANSPFTEGKPNDFLSYRSHIWSDTDPHRT
GMLPFVFEDGFGYERWTDYMLDVPMYFVYRDGKYIDAAGHSFRDFLDGKLSVLPGEKPRPSDWWDHLSTAFPEVRLKSFL
EMRGADGGPWSRICALPALWVGLLYDDSALDAAWDLVKDWSMEEREALRNAVPRLALDAPIPGGHRLQDLARDVLKIARQ
GLTARARLGESGDNETGFLSTLDEIVETGKVPAQRLLDMYHGEWGGDIKRVYKYSF
>Mature_455_residues
STREDSGADEPIIESRDQLVAPMQAGEKPKDRWRIGTEHEKLVYKRDGFRAPSYDEDGGIRDILLEMQQFGWQPVEEDGK
VIALKGEDGAVSLEPAGQLELSGAPLENLHQTCNETGRHLTQVKAIGEKCGVGFLGLGMWPDKTREELPVMPKGRYDIMM
RHMPRVGSLGLDMMLRTCTIQVNLDYSSEADMAKKFRTSLALQPLATALFANSPFTEGKPNDFLSYRSHIWSDTDPHRTG
MLPFVFEDGFGYERWTDYMLDVPMYFVYRDGKYIDAAGHSFRDFLDGKLSVLPGEKPRPSDWWDHLSTAFPEVRLKSFLE
MRGADGGPWSRICALPALWVGLLYDDSALDAAWDLVKDWSMEEREALRNAVPRLALDAPIPGGHRLQDLARDVLKIARQG
LTARARLGESGDNETGFLSTLDEIVETGKVPAQRLLDMYHGEWGGDIKRVYKYSF

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 51412; Mature: 51281

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTREDSGADEPIIESRDQLVAPMQAGEKPKDRWRIGTEHEKLVYKRDGFRAPSYDEDGG
CCCCCCCCCCCCHHHCHHHHCCHHHCCCCCHHHCCCCCCHHHHEEECCCCCCCCCCCCCC
IRDILLEMQQFGWQPVEEDGKVIALKGEDGAVSLEPAGQLELSGAPLENLHQTCNETGRH
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LTQVKAIGEKCGVGFLGLGMWPDKTREELPVMPKGRYDIMMRHMPRVGSLGLDMMLRTCT
HHHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCCHHHEEEEEE
IQVNLDYSSEADMAKKFRTSLALQPLATALFANSPFTEGKPNDFLSYRSHIWSDTDPHRT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GMLPFVFEDGFGYERWTDYMLDVPMYFVYRDGKYIDAAGHSFRDFLDGKLSVLPGEKPRP
CCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCEEECCCCHHHHHHCCCEEECCCCCCCC
SDWWDHLSTAFPEVRLKSFLEMRGADGGPWSRICALPALWVGLLYDDSALDAAWDLVKDW
CHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
SMEEREALRNAVPRLALDAPIPGGHRLQDLARDVLKIARQGLTARARLGESGDNETGFLS
CHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
TLDEIVETGKVPAQRLLDMYHGEWGGDIKRVYKYSF
HHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
STREDSGADEPIIESRDQLVAPMQAGEKPKDRWRIGTEHEKLVYKRDGFRAPSYDEDGG
CCCCCCCCCCCHHHCHHHHCCHHHCCCCCHHHCCCCCCHHHHEEECCCCCCCCCCCCCC
IRDILLEMQQFGWQPVEEDGKVIALKGEDGAVSLEPAGQLELSGAPLENLHQTCNETGRH
HHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEECCCCCEEECCCCHHHHHHHHHHHHHH
LTQVKAIGEKCGVGFLGLGMWPDKTREELPVMPKGRYDIMMRHMPRVGSLGLDMMLRTCT
HHHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCCHHHEEEEEE
IQVNLDYSSEADMAKKFRTSLALQPLATALFANSPFTEGKPNDFLSYRSHIWSDTDPHRT
EEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
GMLPFVFEDGFGYERWTDYMLDVPMYFVYRDGKYIDAAGHSFRDFLDGKLSVLPGEKPRP
CCCCEEEECCCCHHHHHHHHHCCCEEEEEECCCEEECCCCHHHHHHCCCEEECCCCCCCC
SDWWDHLSTAFPEVRLKSFLEMRGADGGPWSRICALPALWVGLLYDDSALDAAWDLVKDW
CHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
SMEEREALRNAVPRLALDAPIPGGHRLQDLARDVLKIARQGLTARARLGESGDNETGFLS
CHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
TLDEIVETGKVPAQRLLDMYHGEWGGDIKRVYKYSF
HHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA