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The map label for this gene is pdxJ [H]

Identifier: 85373370

GI number: 85373370

Start: 579894

End: 580610

Strand: Reverse

Name: pdxJ [H]

Synonym: ELI_02715

Alternate gene names: 85373370

Gene position: 580610-579894 (Counterclockwise)

Preceding gene: 85373371

Following gene: 85373369

Centisome position: 19.02

GC content: 65.27

Gene sequence:

>717_bases
GTGAACATCGATCACGTCGCCACCATCCGCAATGCACGCGGCGGGGACCATCCCGACCCGGTTCGCGCGGCCGAAATCGT
CGCGGCAGTCGGTGGCGACGGCATCACCGCACACTTGCGCGAAGACCGCCGCCACATTCGCGACGAAGACCTCGCGCGCA
TCCAGGCTGCAACCGACCTACCGCTCAATCTCGAAATGGCTGCGACCGAAGAGATGCTGGCGATCGCGCTTCGCCACCAG
CCGCATGCCGCCTGCATCGTCCCCGAAAAGCGCGAGGAGCGCACGACCGAAGGCGGGCTCGACGCGGCAGGATTGCACAA
CACCCTCGTGCCGATTGTCGACAAGCTCCGCGAGGCGGACATTCGCGTGTCGCTGTTTATCGAAGCGGACGAGCGCCAGC
TCGATGCCGCGCTGCGCCTCGGCGCACCGGTGGTGGAGTTCCACACGGGCGAATATGCCCATGCCCATCTCGACGGGGAT
CGCGACAAAACGGCGAGCGAGCTCAAGCGCATCGCCGACATGTCCGCGCTGGCCGCCAAGAACGGTATCGAACCCCATGC
CGGGCACGGGCTGACCTACGAAAACGTCCAACCGATCGCCGCCATCCCGCAACTGGCCGAACTCAATATTGGCCACTATC
TGATCGGCGAGGCGGTCTTCGTCGGCCTGGAGAATGCCGTGCGCAGAATGCGCGAGCTCATGGACGATGCGCGGTGA

Upstream 100 bases:

>100_bases
ACGGCGAAGTGCCCGAGGGACTGGCCAGGATCCCCGTCACCAAGCCGGGCAGCCGGGCGAAGTGACTCAGCCGCTCCGCC
ACCATGCTTTGAGATTGGGC

Downstream 100 bases:

>100_bases
TGGTTGGCCACTATACAACTCCGTCGCCCCGGCCTCCGAGCCGGGGTCCCGCTCCCTTGGGACCTGCGCTTGAAGAAAGC
GGGACCCCGGATCAGGTCCG

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNIDHVATIRNARGGDHPDPVRAAEIVAAVGGDGITAHLREDRRHIRDEDLARIQAATDLPLNLEMAATEEMLAIALRHQ
PHAACIVPEKREERTTEGGLDAAGLHNTLVPIVDKLREADIRVSLFIEADERQLDAALRLGAPVVEFHTGEYAHAHLDGD
RDKTASELKRIADMSALAAKNGIEPHAGHGLTYENVQPIAAIPQLAELNIGHYLIGEAVFVGLENAVRRMRELMDDAR

Sequences:

>Translated_238_residues
MNIDHVATIRNARGGDHPDPVRAAEIVAAVGGDGITAHLREDRRHIRDEDLARIQAATDLPLNLEMAATEEMLAIALRHQ
PHAACIVPEKREERTTEGGLDAAGLHNTLVPIVDKLREADIRVSLFIEADERQLDAALRLGAPVVEFHTGEYAHAHLDGD
RDKTASELKRIADMSALAAKNGIEPHAGHGLTYENVQPIAAIPQLAELNIGHYLIGEAVFVGLENAVRRMRELMDDAR
>Mature_238_residues
MNIDHVATIRNARGGDHPDPVRAAEIVAAVGGDGITAHLREDRRHIRDEDLARIQAATDLPLNLEMAATEEMLAIALRHQ
PHAACIVPEKREERTTEGGLDAAGLHNTLVPIVDKLREADIRVSLFIEADERQLDAALRLGAPVVEFHTGEYAHAHLDGD
RDKTASELKRIADMSALAAKNGIEPHAGHGLTYENVQPIAAIPQLAELNIGHYLIGEAVFVGLENAVRRMRELMDDAR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=238, Percent_Identity=55.8823529411765, Blast_Score=257, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 25995; Mature: 25995

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIDHVATIRNARGGDHPDPVRAAEIVAAVGGDGITAHLREDRRHIRDEDLARIQAATDL
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHCCC
PLNLEMAATEEMLAIALRHQPHAACIVPEKREERTTEGGLDAAGLHNTLVPIVDKLREAD
CCCEEHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
IRVSLFIEADERQLDAALRLGAPVVEFHTGEYAHAHLDGDRDKTASELKRIADMSALAAK
EEEEEEEECCHHHHHHHHHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
NGIEPHAGHGLTYENVQPIAAIPQLAELNIGHYLIGEAVFVGLENAVRRMRELMDDAR
CCCCCCCCCCCCCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIDHVATIRNARGGDHPDPVRAAEIVAAVGGDGITAHLREDRRHIRDEDLARIQAATDL
CCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHCCC
PLNLEMAATEEMLAIALRHQPHAACIVPEKREERTTEGGLDAAGLHNTLVPIVDKLREAD
CCCEEHHHHHHHHHHHHHCCCCEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
IRVSLFIEADERQLDAALRLGAPVVEFHTGEYAHAHLDGDRDKTASELKRIADMSALAAK
EEEEEEEECCHHHHHHHHHCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
NGIEPHAGHGLTYENVQPIAAIPQLAELNIGHYLIGEAVFVGLENAVRRMRELMDDAR
CCCCCCCCCCCCCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA