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The map label for this gene is thiL [H]

Identifier: 85373344

GI number: 85373344

Start: 559472

End: 560344

Strand: Reverse

Name: thiL [H]

Synonym: ELI_02585

Alternate gene names: 85373344

Gene position: 560344-559472 (Counterclockwise)

Preceding gene: 85373345

Following gene: 85373341

Centisome position: 18.36

GC content: 66.67

Gene sequence:

>873_bases
GTGACCAACGAGTTCGACTTCATCGCGCGATTGCGCGCGCTGGCCACCGATCCGGCGGCGCGGGGCCTCAACGACGACGC
GGCGGTGCTGGAAATCGGCGGGCAATTGCTGGTCGTCACGCACGACATGATGATCGAGGGCGTCCACTGGCTGCAGGGTC
AGGACATGGCCGATGTCGCGTGGAAACTGGTGGCGGTGAACCTCTCCGATCTCGCGGCAAAGGGTGCGCGGCCGATCGGG
ATTATTGTGGGCCAAACCATCCCGGACGAGGAAGAGCGCTTTTTGAGCGGCCTCAGGGATGCGCTCAGGGCCTTCAACGT
GCCGCTTCTCGGCGGCGATACCGTCAGCGCGGGCCAGGGGCGAACAGCCTTGGGCCTGACTGCGCTCGGGAGTTCCGTCA
GCGGGATCGTGCCCGGTCGCGACGGCGCGCAACCCGGCGACCTCCTCTACATCACCGGCCCTGTCGGCGCGGCAATGATG
GGCTTCGAAGCGCTGCAAGCCGGCACCGAGGACAACAGCCTCGCGTTTCGCCGCCCGATGCCGCTGCTTGCCGAAGGTGC
TGCGCTGGCGCCGCATGTGAGTGCGATGATGGATGTCTCGGACGGGCTGTTGCTCGATGCGTGGCGCCTTGCAACCACCA
GCGGCGTAACGCTCGCCATCGACACCGCGGAAGTTCCCCTCGGGGCACCGGAGGAGCGCCGCGACGACGCGCTGCGCTGG
GGTGACGATTATCAGCTGCTCTTCACGGCATCGCCGACGGCAGAGCTTCCCGCCAGGGCCATTCCCATCGGAACGGTCGA
GGAAGGCGAACCGCCTCTGCTGGTCGATGGTATCGCGATTTTCGAACCCGAGGGGCTCGGCTACCGGCATTGA

Upstream 100 bases:

>100_bases
CCGCGATCAGCGAGTATGTCGATGTGGCCAAGGCCTTCTTCGACGATCGCGAAGCGAAGTTCGTCAATGGCCTACTCGAC
GCGGTCGCCAAACAGGTGCG

Downstream 100 bases:

>100_bases
TACTCAGAGTGCCGAGCAATCCACGCCGCGCTTTTCCAGGACCGGTTTCATCTCCTGATACGCCGCCTTGGTCCGGTGGT
TCGGAATTCCCGGATACAAG

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTNEFDFIARLRALATDPAARGLNDDAAVLEIGGQLLVVTHDMMIEGVHWLQGQDMADVAWKLVAVNLSDLAAKGARPIG
IIVGQTIPDEEERFLSGLRDALRAFNVPLLGGDTVSAGQGRTALGLTALGSSVSGIVPGRDGAQPGDLLYITGPVGAAMM
GFEALQAGTEDNSLAFRRPMPLLAEGAALAPHVSAMMDVSDGLLLDAWRLATTSGVTLAIDTAEVPLGAPEERRDDALRW
GDDYQLLFTASPTAELPARAIPIGTVEEGEPPLLVDGIAIFEPEGLGYRH

Sequences:

>Translated_290_residues
MTNEFDFIARLRALATDPAARGLNDDAAVLEIGGQLLVVTHDMMIEGVHWLQGQDMADVAWKLVAVNLSDLAAKGARPIG
IIVGQTIPDEEERFLSGLRDALRAFNVPLLGGDTVSAGQGRTALGLTALGSSVSGIVPGRDGAQPGDLLYITGPVGAAMM
GFEALQAGTEDNSLAFRRPMPLLAEGAALAPHVSAMMDVSDGLLLDAWRLATTSGVTLAIDTAEVPLGAPEERRDDALRW
GDDYQLLFTASPTAELPARAIPIGTVEEGEPPLLVDGIAIFEPEGLGYRH
>Mature_289_residues
TNEFDFIARLRALATDPAARGLNDDAAVLEIGGQLLVVTHDMMIEGVHWLQGQDMADVAWKLVAVNLSDLAAKGARPIGI
IVGQTIPDEEERFLSGLRDALRAFNVPLLGGDTVSAGQGRTALGLTALGSSVSGIVPGRDGAQPGDLLYITGPVGAAMMG
FEALQAGTEDNSLAFRRPMPLLAEGAALAPHVSAMMDVSDGLLLDAWRLATTSGVTLAIDTAEVPLGAPEERRDDALRWG
DDYQLLFTASPTAELPARAIPIGTVEEGEPPLLVDGIAIFEPEGLGYRH

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=277, Percent_Identity=35.7400722021661, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 30455; Mature: 30324

Theoretical pI: Translated: 4.11; Mature: 4.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNEFDFIARLRALATDPAARGLNDDAAVLEIGGQLLVVTHDMMIEGVHWLQGQDMADVA
CCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCEEEEEEEHHHHCCHHHCCCCCHHHHE
WKLVAVNLSDLAAKGARPIGIIVGQTIPDEEERFLSGLRDALRAFNVPLLGGDTVSAGQG
EEEEEECHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC
RTALGLTALGSSVSGIVPGRDGAQPGDLLYITGPVGAAMMGFEALQAGTEDNSLAFRRPM
CCEEEHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCC
PLLAEGAALAPHVSAMMDVSDGLLLDAWRLATTSGVTLAIDTAEVPLGAPEERRDDALRW
CHHHCCCCCCHHHHHHHHCCCCEEEEHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHCC
GDDYQLLFTASPTAELPARAIPIGTVEEGEPPLLVDGIAIFEPEGLGYRH
CCCEEEEEECCCCCCCCCCEECCCCCCCCCCCEEECCEEEECCCCCCCCC
>Mature Secondary Structure 
TNEFDFIARLRALATDPAARGLNDDAAVLEIGGQLLVVTHDMMIEGVHWLQGQDMADVA
CCHHHHHHHHHHHHCCHHHCCCCCCCEEEEECCEEEEEEEHHHHCCHHHCCCCCHHHHE
WKLVAVNLSDLAAKGARPIGIIVGQTIPDEEERFLSGLRDALRAFNVPLLGGDTVSAGQG
EEEEEECHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCC
RTALGLTALGSSVSGIVPGRDGAQPGDLLYITGPVGAAMMGFEALQAGTEDNSLAFRRPM
CCEEEHHHHCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCC
PLLAEGAALAPHVSAMMDVSDGLLLDAWRLATTSGVTLAIDTAEVPLGAPEERRDDALRW
CHHHCCCCCCHHHHHHHHCCCCEEEEHHHHHCCCCEEEEEECCCCCCCCCHHHHCCHHCC
GDDYQLLFTASPTAELPARAIPIGTVEEGEPPLLVDGIAIFEPEGLGYRH
CCCEEEEEECCCCCCCCCCEECCCCCCCCCCCEEECCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]