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The map label for this gene is purC

Identifier: 85373319

GI number: 85373319

Start: 531446

End: 532264

Strand: Reverse

Name: purC

Synonym: ELI_02460

Alternate gene names: 85373319

Gene position: 532264-531446 (Counterclockwise)

Preceding gene: 85373321

Following gene: 85373317

Centisome position: 17.44

GC content: 60.68

Gene sequence:

>819_bases
ATGTCGCGCCGCCGCCAGATCTACGAAGGCAAGGCCAAGATCCTTTACGAGGGTCCGGAGCCGGGCACCATCATCCAGTA
TTTCAAGGACGATGCCACTGCTTTCAACGCCGAGAAAAAAGGCACGATCAGCGGCAAGGGGGTGATCAATAACCGCATCA
GCGAGCATGTGTTCACGCGGCTCAGCCACATCGGCATCCCGACGCATTTCATCCGCCGCCTCAACATGCGCGAACAGCTG
GTCCGCCAGTGCGAGATCATCCCGATCGAAGTCGTGGTGCGCAATGTCGCCGCCGGATCGATCAGCAAGCGGCTGGGCAT
CCCCGAAGGCGAGCCGCTGCCGCACACGCTGATCGAATATTACTATAAGGACGATTCGCTCGGCGATCCGCTAATTGCTG
AAGAACATATCGCCTGTTTCAACTGGGCGAGCCCTGAGGAAATGCAGGACATCGCCAGCATGGCGATCCGCATCAACGAT
TTCATGGCCGGCATGTTCGCGGCGATCGACATCCGGCTGGTCGATTTCAAGCTGGAATTCGGACGCATCTATGATGGTGA
CTTCAGCCGCGTGATCCTGGCCGACGAGATCAGTCCCGACGGCTGCCGCCTGTGGGACATGAATTCGGGCGAGAAGCTCG
ACAAGGATCGCTTCCGCCGCGATCTCGGCGGGGAGAGCGAGGCTTACCAGGAAGTCGCGCGGCGGCTCGGCCTGCTCGAC
GAGGACAGGAACAACGGCGAAGGCGGTCCCGGCGAAGTCTTCGACATGTCGGCCCATCGCAGCCGTCTGCGCGGCACGCC
GACCCCGCCGAAAAAATAG

Upstream 100 bases:

>100_bases
ACAAGGCCGCGTTCCGCTGTAAACGCTTCCGTCGCCCCGGTAGCCGAAGCACACGCTTCGCGTGCCGGGGCCATCTGTTT
CAAGCCGAGGGACCACCGCC

Downstream 100 bases:

>100_bases
GGGCTAGACCACGTAGGTAAGCGTGATGCCGCTCGGCTCGATGGCGAAGGCGCAATCGGGCATGTGGTCGCCGTCGACCT
GCACCGGCGTGATGCAGCGG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MSRRRQIYEGKAKILYEGPEPGTIIQYFKDDATAFNAEKKGTISGKGVINNRISEHVFTRLSHIGIPTHFIRRLNMREQL
VRQCEIIPIEVVVRNVAAGSISKRLGIPEGEPLPHTLIEYYYKDDSLGDPLIAEEHIACFNWASPEEMQDIASMAIRIND
FMAGMFAAIDIRLVDFKLEFGRIYDGDFSRVILADEISPDGCRLWDMNSGEKLDKDRFRRDLGGESEAYQEVARRLGLLD
EDRNNGEGGPGEVFDMSAHRSRLRGTPTPPKK

Sequences:

>Translated_272_residues
MSRRRQIYEGKAKILYEGPEPGTIIQYFKDDATAFNAEKKGTISGKGVINNRISEHVFTRLSHIGIPTHFIRRLNMREQL
VRQCEIIPIEVVVRNVAAGSISKRLGIPEGEPLPHTLIEYYYKDDSLGDPLIAEEHIACFNWASPEEMQDIASMAIRIND
FMAGMFAAIDIRLVDFKLEFGRIYDGDFSRVILADEISPDGCRLWDMNSGEKLDKDRFRRDLGGESEAYQEVARRLGLLD
EDRNNGEGGPGEVFDMSAHRSRLRGTPTPPKK
>Mature_271_residues
SRRRQIYEGKAKILYEGPEPGTIIQYFKDDATAFNAEKKGTISGKGVINNRISEHVFTRLSHIGIPTHFIRRLNMREQLV
RQCEIIPIEVVVRNVAAGSISKRLGIPEGEPLPHTLIEYYYKDDSLGDPLIAEEHIACFNWASPEEMQDIASMAIRINDF
MAGMFAAIDIRLVDFKLEFGRIYDGDFSRVILADEISPDGCRLWDMNSGEKLDKDRFRRDLGGESEAYQEVARRLGLLDE
DRNNGEGGPGEVFDMSAHRSRLRGTPTPPKK

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Homo sapiens, GI119220557, Length=251, Percent_Identity=33.8645418326693, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI5453539, Length=251, Percent_Identity=33.8645418326693, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI119220559, Length=258, Percent_Identity=32.5581395348837, Blast_Score=105, Evalue=3e-23,
Organism=Escherichia coli, GI1788820, Length=238, Percent_Identity=41.5966386554622, Blast_Score=200, Evalue=9e-53,
Organism=Caenorhabditis elegans, GI17531275, Length=222, Percent_Identity=32.8828828828829, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18860083, Length=239, Percent_Identity=29.7071129707113, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24583917, Length=207, Percent_Identity=31.4009661835749, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_ERYLH (Q2NCK7)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457381.1
- HSSP:   P0A7D7
- ProteinModelPortal:   Q2NCK7
- SMR:   Q2NCK7
- STRING:   Q2NCK7
- GeneID:   3871445
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_02460
- NMPDR:   fig|314225.3.peg.2593
- eggNOG:   COG0152
- HOGENOM:   HBG306070
- OMA:   YKDDALG
- PhylomeDB:   Q2NCK7
- ProtClustDB:   PRK09362
- BioCyc:   ELIT314225:ELI_02460-MONOMER
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 30831; Mature: 30700

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRQIYEGKAKILYEGPEPGTIIQYFKDDATAFNAEKKGTISGKGVINNRISEHVFTR
CCCCCCHHCCCEEEEEECCCCCCEEEEECCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHH
LSHIGIPTHFIRRLNMREQLVRQCEIIPIEVVVRNVAAGSISKRLGIPEGEPLPHTLIEY
HHHCCCCHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
YYKDDSLGDPLIAEEHIACFNWASPEEMQDIASMAIRINDFMAGMFAAIDIRLVDFKLEF
HHCCCCCCCCCHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEE
GRIYDGDFSRVILADEISPDGCRLWDMNSGEKLDKDRFRRDLGGESEAYQEVARRLGLLD
CEEECCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCC
EDRNNGEGGPGEVFDMSAHRSRLRGTPTPPKK
CCCCCCCCCCCCEEECHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRRRQIYEGKAKILYEGPEPGTIIQYFKDDATAFNAEKKGTISGKGVINNRISEHVFTR
CCCCCHHCCCEEEEEECCCCCCEEEEECCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHH
LSHIGIPTHFIRRLNMREQLVRQCEIIPIEVVVRNVAAGSISKRLGIPEGEPLPHTLIEY
HHHCCCCHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
YYKDDSLGDPLIAEEHIACFNWASPEEMQDIASMAIRINDFMAGMFAAIDIRLVDFKLEF
HHCCCCCCCCCHHHCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEE
GRIYDGDFSRVILADEISPDGCRLWDMNSGEKLDKDRFRRDLGGESEAYQEVARRLGLLD
CEEECCCCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCCC
EDRNNGEGGPGEVFDMSAHRSRLRGTPTPPKK
CCCCCCCCCCCCEEECHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA