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The map label for this gene is zraR [H]

Identifier: 85373292

GI number: 85373292

Start: 505455

End: 506873

Strand: Reverse

Name: zraR [H]

Synonym: ELI_02325

Alternate gene names: 85373292

Gene position: 506873-505455 (Counterclockwise)

Preceding gene: 85373306

Following gene: 85373291

Centisome position: 16.61

GC content: 66.6

Gene sequence:

>1419_bases
ATGGCCGAAGACGAAACGCGCCTCTTGATGTTGATCGACGACGAACCGGCGCAGAGCCGGTTGATCGGCGCGCTCGCCGC
CCGCGAGGGCTGGCGCACGCTGGTCGTCGCCGACGCCGAGACCGCGATTGCGACACTCGGCACGCGTCAGGGCATGCAGC
TTTCCGCCATCATCCTTGACCAGTGGGTCCCGGGCGACGATGCCTGCCAGCTGATCGGGGAATTGAAGAGCCGCCGCCCG
GCCCTGCCGATCCTGATGCTCACGCAAAGCGCCAGTCCGCTGCTCGCCGTCGAAGCGATGCGGGCCGGGGCGACCGACTA
TCTCATCAAGCCCGTCGCACCCGAACGGCTGATGCGCGCGCTGCGCAGCGCCACCAAGCGCGAGGCACCGAAGGACGAAC
TCCAGCCGCTGACGGAAAAGATGCCCGCGGTGCTCGATTTCGATGCCATGATCGGCACCGAGCCCGCCTTCCGCACCGCG
CTCGCCCGCGCCGCCAAGATCGCGCGCGGCCATGGCCACGTGCTGATCGAAGGCGAAAGCGGCACCGGCAAGGAAATGCT
TTTACGCGCCATGCATGCGGCCAGCCCGCGTGCCAAGGCGCCGTTCCGCATGATCAATATCGGCGGCATTCCCGCCAATT
CGATCGAATCCGCGCTGTTCGGGCATGAGCAAAACGCTTTCCCGGGCGCTTTCGACCGCCAGGTCGGCGCGCTCCAGTTC
TGCGACGGCGGCACGCTCATCATCGACGAGATCGATCGCCTGAGCGGCGAACTGCAACAGCGGCTTTCGGCCACGCTGGC
AGAAGGTATCGTGCGCCCGATCGGGGCCAAGCACGGCTTCAAGATCGACATTCGCCTGCTCACGGCAAGCAATCTGCCGC
TCGAAGACCTGGTCGAAGCCGGCCATTTCGAAGCGGACCTGCTGGAACAGATCGCCGCGACCAAGATCTGCCTGCCGCCC
TTGCGCGAGCGGCCGGGTGACATCCCGGCGCTGGCCCGCCACTTCCTCGGCCGCATCGGCGAACAGCCGGGTCTCAAGCA
CCTGTCGCTCGCCGACAGTGCGCTCGGCCTGCTCGGTGCCTATGACTGGCCGGGCAACGTCCGCCAGTTGCAGGCCGTGC
TGTTCCGCGCCGCTGTGTTCTGCGAAGGCGAAGCGTTGACCTCGCGAGATTTCCCGCAGCTGTCCGAGATCGTCGGCGAA
CTCGCCGAGACCGGTGCCAGCCTGCCCGAGGCCGTCGGCGTCATGCTCTACACCGAAGATGGCAATCTGCGCCCGCTCGA
CGAGATCGAAGCCGACGTGATCCGTCTAGCCATCGGCCACTATCGCGGCCGCATGACCGAAGTCGCCCGTCGACTTGGGA
TCGGGCGTTCGACGCTCTATCGCAAGCTGAGCGATCTCGGCATCGACAACGCGGCCTAG

Upstream 100 bases:

>100_bases
TGCCAAGGGTGAAGCGCGGCGAACGATTAGGCTTAATCGGAACTTTACCCCTTCCCGCTAATCCGGGGTGCTGTGCCGCA
CTGGGACGGTTTGGGGAACC

Downstream 100 bases:

>100_bases
GAACACATCGCAATGCCTGATGGCCCCGCACCCAAGCTCGAAGCAGCCGAAAGCGCTGCCCCGGCAGATGATAGTTTCCG
CGACGCTCTCGATCGCCTGT

Product: sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAEDETRLLMLIDDEPAQSRLIGALAAREGWRTLVVADAETAIATLGTRQGMQLSAIILDQWVPGDDACQLIGELKSRRP
ALPILMLTQSASPLLAVEAMRAGATDYLIKPVAPERLMRALRSATKREAPKDELQPLTEKMPAVLDFDAMIGTEPAFRTA
LARAAKIARGHGHVLIEGESGTGKEMLLRAMHAASPRAKAPFRMINIGGIPANSIESALFGHEQNAFPGAFDRQVGALQF
CDGGTLIIDEIDRLSGELQQRLSATLAEGIVRPIGAKHGFKIDIRLLTASNLPLEDLVEAGHFEADLLEQIAATKICLPP
LRERPGDIPALARHFLGRIGEQPGLKHLSLADSALGLLGAYDWPGNVRQLQAVLFRAAVFCEGEALTSRDFPQLSEIVGE
LAETGASLPEAVGVMLYTEDGNLRPLDEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLSDLGIDNAA

Sequences:

>Translated_472_residues
MAEDETRLLMLIDDEPAQSRLIGALAAREGWRTLVVADAETAIATLGTRQGMQLSAIILDQWVPGDDACQLIGELKSRRP
ALPILMLTQSASPLLAVEAMRAGATDYLIKPVAPERLMRALRSATKREAPKDELQPLTEKMPAVLDFDAMIGTEPAFRTA
LARAAKIARGHGHVLIEGESGTGKEMLLRAMHAASPRAKAPFRMINIGGIPANSIESALFGHEQNAFPGAFDRQVGALQF
CDGGTLIIDEIDRLSGELQQRLSATLAEGIVRPIGAKHGFKIDIRLLTASNLPLEDLVEAGHFEADLLEQIAATKICLPP
LRERPGDIPALARHFLGRIGEQPGLKHLSLADSALGLLGAYDWPGNVRQLQAVLFRAAVFCEGEALTSRDFPQLSEIVGE
LAETGASLPEAVGVMLYTEDGNLRPLDEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLSDLGIDNAA
>Mature_471_residues
AEDETRLLMLIDDEPAQSRLIGALAAREGWRTLVVADAETAIATLGTRQGMQLSAIILDQWVPGDDACQLIGELKSRRPA
LPILMLTQSASPLLAVEAMRAGATDYLIKPVAPERLMRALRSATKREAPKDELQPLTEKMPAVLDFDAMIGTEPAFRTAL
ARAAKIARGHGHVLIEGESGTGKEMLLRAMHAASPRAKAPFRMINIGGIPANSIESALFGHEQNAFPGAFDRQVGALQFC
DGGTLIIDEIDRLSGELQQRLSATLAEGIVRPIGAKHGFKIDIRLLTASNLPLEDLVEAGHFEADLLEQIAATKICLPPL
RERPGDIPALARHFLGRIGEQPGLKHLSLADSALGLLGAYDWPGNVRQLQAVLFRAAVFCEGEALTSRDFPQLSEIVGEL
AETGASLPEAVGVMLYTEDGNLRPLDEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLSDLGIDNAA

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=464, Percent_Identity=32.7586206896552, Blast_Score=244, Evalue=7e-66,
Organism=Escherichia coli, GI1790437, Length=457, Percent_Identity=35.0109409190372, Blast_Score=241, Evalue=6e-65,
Organism=Escherichia coli, GI1790299, Length=429, Percent_Identity=36.1305361305361, Blast_Score=232, Evalue=4e-62,
Organism=Escherichia coli, GI1788905, Length=461, Percent_Identity=32.1041214750542, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082117, Length=353, Percent_Identity=34.5609065155807, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789087, Length=352, Percent_Identity=35.2272727272727, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI87082152, Length=322, Percent_Identity=34.7826086956522, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=31.6923076923077, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=32.4159021406728, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI87081872, Length=252, Percent_Identity=34.5238095238095, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786524, Length=335, Percent_Identity=32.5373134328358, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081858, Length=323, Percent_Identity=29.1021671826625, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789828, Length=267, Percent_Identity=28.8389513108614, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50976; Mature: 50844

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDETRLLMLIDDEPAQSRLIGALAAREGWRTLVVADAETAIATLGTRQGMQLSAIILD
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEE
QWVPGDDACQLIGELKSRRPALPILMLTQSASPLLAVEAMRAGATDYLIKPVAPERLMRA
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCHHEECCCCHHHHHHH
LRSATKREAPKDELQPLTEKMPAVLDFDAMIGTEPAFRTALARAAKIARGHGHVLIEGES
HHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GTGKEMLLRAMHAASPRAKAPFRMINIGGIPANSIESALFGHEQNAFPGAFDRQVGALQF
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHCCCCCEEE
CDGGTLIIDEIDRLSGELQQRLSATLAEGIVRPIGAKHGFKIDIRLLTASNLPLEDLVEA
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHC
GHFEADLLEQIAATKICLPPLRERPGDIPALARHFLGRIGEQPGLKHLSLADSALGLLGA
CCCHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
YDWPGNVRQLQAVLFRAAVFCEGEALTSRDFPQLSEIVGELAETGASLPEAVGVMLYTED
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCEEEEEECC
GNLRPLDEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLSDLGIDNAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AEDETRLLMLIDDEPAQSRLIGALAAREGWRTLVVADAETAIATLGTRQGMQLSAIILD
CCCCCEEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEE
QWVPGDDACQLIGELKSRRPALPILMLTQSASPLLAVEAMRAGATDYLIKPVAPERLMRA
CCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCCCHHEECCCCHHHHHHH
LRSATKREAPKDELQPLTEKMPAVLDFDAMIGTEPAFRTALARAAKIARGHGHVLIEGES
HHHHHHCCCCHHHHHHHHHHCCCCEEHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCC
GTGKEMLLRAMHAASPRAKAPFRMINIGGIPANSIESALFGHEQNAFPGAFDRQVGALQF
CCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCHHHCCCCCEEE
CDGGTLIIDEIDRLSGELQQRLSATLAEGIVRPIGAKHGFKIDIRLLTASNLPLEDLVEA
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHHHC
GHFEADLLEQIAATKICLPPLRERPGDIPALARHFLGRIGEQPGLKHLSLADSALGLLGA
CCCHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCC
YDWPGNVRQLQAVLFRAAVFCEGEALTSRDFPQLSEIVGELAETGASLPEAVGVMLYTED
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHCEEEEEECC
GNLRPLDEIEADVIRLAIGHYRGRMTEVARRLGIGRSTLYRKLSDLGIDNAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]