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The map label for this gene is cca [H]

Identifier: 85373276

GI number: 85373276

Start: 489058

End: 490245

Strand: Reverse

Name: cca [H]

Synonym: ELI_02245

Alternate gene names: 85373276

Gene position: 490245-489058 (Counterclockwise)

Preceding gene: 85373277

Following gene: 85373275

Centisome position: 16.06

GC content: 68.52

Gene sequence:

>1188_bases
ATGCAGCAAATCTTGCCATCAGCCCCATGGACCCAGCGCGACGACCTCGCGTCGCTGGTCGGTGTGCTGGGCGCCGACAA
TCTGCGCTGGGTGGGAGGCGCGGTGCGCGACACGCTGCTGGGCGATGCTGTCAAGGATGTCGATGCGGCGACGCCGCTGT
TGCCTGAGGATGTGATGGAGCGCTTGGCCGCTGCCGACATCCGCACCGTCCCGACAGGCATCGATCACGGCACCGTCACC
GCCATCCTGCCGGGCGGGCCGGTCGAGATCACGACCCTGCGCAAGGATGTCAGTACCGATGGGCGCCGCGCCACCGTCGC
CTTTGCAGGCGAGTGGCGCGATGACGCGGCGCGGCGCGATTTCACCATCAACGCGCTGTACGCTGACCCGAAGACCTTGG
CGATCAGCGATTTTTTCGGCGGCCTCGAGGACCTTGAGGCGCGCCGTGTCCGCTTTATCGGCGACGCGCGTGAACGTATC
CGTGAAGACCACCTGCGTATTCTCCGCTATTTCCGCTTCCAGGCGCGGTTCGGCGCGGAGCTGGACGAAGAGGCCGAGGA
AGCCTGCGCCGAGCTTGCCGAAACGCTCAAGGGCCTGAGCCGCGAGCGCGTGGCGATGGAATTGCTGAGCCTGCTCGCCC
TGCCCGATCCGGCGCCTACGATCGCGCGGATGCGCGAGCGCGGGGTGCTGCCCGTCATCTTGTCCGAAGCCGGCAAGGCG
GAAGTCGCGCGGCTGCAAGCCCTGATTGCCAGCGAGGCTCGATACTCCATCGCGCCCGATCCCTTGCGGCGGCTCGCCGC
GCTTCTGCCTCCGGTGGCGACGCTGGCCGAAAGCGTCGCGGCGCGGCTGCGCCTGTCGCGAGCCCAACGCGACCGGCTGG
TCTCCTTGGCCCGGCGACAGCCCGAAGACGAGACGCGCGCACGACCGCTGGCCTATGAAGAAGGTCTCGATAATGCACGG
GACAGGCTGCTGCTGGCGGGCGCAGACCCCGCGCCCCTGCAAGACTGGCATGTCCCGCAATTCCCACTCAAGGGCGGTGC
GATCGTCGCGCGCGGGGTCGGGGCCGGCCCGGAGGTTGCGCGGATCCTGCAGGGCATAGAGGCGCGGTGGGTCGAAGAGG
GGTTCCCGCCCGAAGCGCGGGTTGCCGAGCTGCTCGACGAAGCCCTGAACGCCCGCGATTTGCCTTAA

Upstream 100 bases:

>100_bases
TCCGTTCACCCTGAGCTTGTCGAAGGGTCGTACTTTCTTATAGCGCAGGGTTATTAGTGAAGGACGGGGCTTGGACAGGC
GCAGCCCGAACGGATGTTGG

Downstream 100 bases:

>100_bases
GCCGAGCTTCAGGTGCGATAAGCTACCTATCCAAATGTTGTCATGCGGGAACCGATCCCGTTATGGCGCGCTCTTCCGGT
GACCCCGGAATCCAATCAGG

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MQQILPSAPWTQRDDLASLVGVLGADNLRWVGGAVRDTLLGDAVKDVDAATPLLPEDVMERLAAADIRTVPTGIDHGTVT
AILPGGPVEITTLRKDVSTDGRRATVAFAGEWRDDAARRDFTINALYADPKTLAISDFFGGLEDLEARRVRFIGDARERI
REDHLRILRYFRFQARFGAELDEEAEEACAELAETLKGLSRERVAMELLSLLALPDPAPTIARMRERGVLPVILSEAGKA
EVARLQALIASEARYSIAPDPLRRLAALLPPVATLAESVAARLRLSRAQRDRLVSLARRQPEDETRARPLAYEEGLDNAR
DRLLLAGADPAPLQDWHVPQFPLKGGAIVARGVGAGPEVARILQGIEARWVEEGFPPEARVAELLDEALNARDLP

Sequences:

>Translated_395_residues
MQQILPSAPWTQRDDLASLVGVLGADNLRWVGGAVRDTLLGDAVKDVDAATPLLPEDVMERLAAADIRTVPTGIDHGTVT
AILPGGPVEITTLRKDVSTDGRRATVAFAGEWRDDAARRDFTINALYADPKTLAISDFFGGLEDLEARRVRFIGDARERI
REDHLRILRYFRFQARFGAELDEEAEEACAELAETLKGLSRERVAMELLSLLALPDPAPTIARMRERGVLPVILSEAGKA
EVARLQALIASEARYSIAPDPLRRLAALLPPVATLAESVAARLRLSRAQRDRLVSLARRQPEDETRARPLAYEEGLDNAR
DRLLLAGADPAPLQDWHVPQFPLKGGAIVARGVGAGPEVARILQGIEARWVEEGFPPEARVAELLDEALNARDLP
>Mature_395_residues
MQQILPSAPWTQRDDLASLVGVLGADNLRWVGGAVRDTLLGDAVKDVDAATPLLPEDVMERLAAADIRTVPTGIDHGTVT
AILPGGPVEITTLRKDVSTDGRRATVAFAGEWRDDAARRDFTINALYADPKTLAISDFFGGLEDLEARRVRFIGDARERI
REDHLRILRYFRFQARFGAELDEEAEEACAELAETLKGLSRERVAMELLSLLALPDPAPTIARMRERGVLPVILSEAGKA
EVARLQALIASEARYSIAPDPLRRLAALLPPVATLAESVAARLRLSRAQRDRLVSLARRQPEDETRARPLAYEEGLDNAR
DRLLLAGADPAPLQDWHVPQFPLKGGAIVARGVGAGPEVARILQGIEARWVEEGFPPEARVAELLDEALNARDLP

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=386, Percent_Identity=34.7150259067358, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI87081691, Length=192, Percent_Identity=34.375, Blast_Score=92, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=433, Percent_Identity=31.8706697459584, Blast_Score=170, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6321016, Length=241, Percent_Identity=29.4605809128631, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281360112, Length=377, Percent_Identity=30.7692307692308, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21357337, Length=377, Percent_Identity=30.7692307692308, Blast_Score=156, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 43184; Mature: 43184

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQILPSAPWTQRDDLASLVGVLGADNLRWVGGAVRDTLLGDAVKDVDAATPLLPEDVME
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RLAAADIRTVPTGIDHGTVTAILPGGPVEITTLRKDVSTDGRRATVAFAGEWRDDAARRD
HHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCCCEEEEEEECCCCCHHHCCC
FTINALYADPKTLAISDFFGGLEDLEARRVRFIGDARERIREDHLRILRYFRFQARFGAE
EEEEEEECCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDEEAEEACAELAETLKGLSRERVAMELLSLLALPDPAPTIARMRERGVLPVILSEAGKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCHH
EVARLQALIASEARYSIAPDPLRRLAALLPPVATLAESVAARLRLSRAQRDRLVSLARRQ
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDETRARPLAYEEGLDNARDRLLLAGADPAPLQDWHVPQFPLKGGAIVARGVGAGPEVA
CCHHHHCCCCHHHHCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
RILQGIEARWVEEGFPPEARVAELLDEALNARDLP
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MQQILPSAPWTQRDDLASLVGVLGADNLRWVGGAVRDTLLGDAVKDVDAATPLLPEDVME
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RLAAADIRTVPTGIDHGTVTAILPGGPVEITTLRKDVSTDGRRATVAFAGEWRDDAARRD
HHHHHHHHHCCCCCCCCCEEEEECCCCEEEEEEHHHCCCCCCEEEEEEECCCCCHHHCCC
FTINALYADPKTLAISDFFGGLEDLEARRVRFIGDARERIREDHLRILRYFRFQARFGAE
EEEEEEECCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LDEEAEEACAELAETLKGLSRERVAMELLSLLALPDPAPTIARMRERGVLPVILSEAGKA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEECCCCCHH
EVARLQALIASEARYSIAPDPLRRLAALLPPVATLAESVAARLRLSRAQRDRLVSLARRQ
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PEDETRARPLAYEEGLDNARDRLLLAGADPAPLQDWHVPQFPLKGGAIVARGVGAGPEVA
CCHHHHCCCCHHHHCCCHHHCCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHH
RILQGIEARWVEEGFPPEARVAELLDEALNARDLP
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11442348 [H]