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The map label for this gene is ysgA [H]

Identifier: 85373248

GI number: 85373248

Start: 463936

End: 464748

Strand: Reverse

Name: ysgA [H]

Synonym: ELI_02105

Alternate gene names: 85373248

Gene position: 464748-463936 (Counterclockwise)

Preceding gene: 85373249

Following gene: 85373247

Centisome position: 15.23

GC content: 66.05

Gene sequence:

>813_bases
GTGACCCGCCGGCAGATCACCGGCTTTTCCAACCCGACGGTCAAGTATCTCCGTTCGCTGCGCGAGAAGAAGCACCGCAA
GCGCGAGCGCAAGTTCCTCGCCGAAGGGTTGCGGTTGCTAACCGATGCGCGCGAATGCGACCGGGTGCCGGAGATGCTGG
TGATGGCCGAACGGCGCGATCCGCACCCGCTCCTCACGGCGCTGGAAGAAGCGGTCACGGGCGCGGGCGGCGAGGTCATC
GAAACCTCCGACGAGATACTCGCCAAGATCACCGGCAAGTCCAACCCGCAAGCGGTCGCAGGCGTATTCGCGGAGTGGAA
CACTTCGCTGTCGGCGCTGGACCGCCAGAGCGCACCGATCTGGCTGGTAGCGCAGGCTCTGCGCGATCCTGGCAACCTCG
GCACCATGCTGCGCACCGGCGATGCCGTGGGGGCTGGCGGGCTTATCCTGCTGGATGATTGCGTCGATCCTTTCAGCGTC
GAAGCCGTGCGCGCCAGCATGGGTGCGGTGTTTACCCAGCAGATCGCCCAAGCCCGCTGGGACGATTTCCTGCTGTGGCT
CCGCAGCGGTGACGGACAATTGGTCGCCGCGTCCCTGCGCGATGCCGTGCCCTATCGCGGCGCCGCCTACGCCGCCCCGT
GTTTCATTCTCGTCGGCAACGAGTCGCAAGGGCTGCCCGAAAATTACGAAACGGCCTGCGACTTGCGGGTGACCATGCCG
ATGCGCGGGCGCGCCGACAGCCTCAACGCGGCCGTCGCCGGAGCGGTATTGGCTTACGAAGTCCTGGACACCTTCGAGAA
CCCGGCCTCCTAA

Upstream 100 bases:

>100_bases
GGCGACACGATCGAGATCATCGTCGCCGGCGACGGCCGGGACCAGGTGCTGGAAGAGCTTTGCACACTGGTCGAAGACGG
TTTCGGCGAGGAGTAGCGCC

Downstream 100 bases:

>100_bases
GCGCCGGTCTTGCCAAAAAGCCGAAACGGCGCTTAGCACTAGGACCGTTTTTTTAGGGTGGATGGGGACGAAATGCAGGA
TTTGCTCGCGAAATATGGCC

Product: rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTRRQITGFSNPTVKYLRSLREKKHRKRERKFLAEGLRLLTDARECDRVPEMLVMAERRDPHPLLTALEEAVTGAGGEVI
ETSDEILAKITGKSNPQAVAGVFAEWNTSLSALDRQSAPIWLVAQALRDPGNLGTMLRTGDAVGAGGLILLDDCVDPFSV
EAVRASMGAVFTQQIAQARWDDFLLWLRSGDGQLVAASLRDAVPYRGAAYAAPCFILVGNESQGLPENYETACDLRVTMP
MRGRADSLNAAVAGAVLAYEVLDTFENPAS

Sequences:

>Translated_270_residues
MTRRQITGFSNPTVKYLRSLREKKHRKRERKFLAEGLRLLTDARECDRVPEMLVMAERRDPHPLLTALEEAVTGAGGEVI
ETSDEILAKITGKSNPQAVAGVFAEWNTSLSALDRQSAPIWLVAQALRDPGNLGTMLRTGDAVGAGGLILLDDCVDPFSV
EAVRASMGAVFTQQIAQARWDDFLLWLRSGDGQLVAASLRDAVPYRGAAYAAPCFILVGNESQGLPENYETACDLRVTMP
MRGRADSLNAAVAGAVLAYEVLDTFENPAS
>Mature_269_residues
TRRQITGFSNPTVKYLRSLREKKHRKRERKFLAEGLRLLTDARECDRVPEMLVMAERRDPHPLLTALEEAVTGAGGEVIE
TSDEILAKITGKSNPQAVAGVFAEWNTSLSALDRQSAPIWLVAQALRDPGNLGTMLRTGDAVGAGGLILLDDCVDPFSVE
AVRASMGAVFTQQIAQARWDDFLLWLRSGDGQLVAASLRDAVPYRGAAYAAPCFILVGNESQGLPENYETACDLRVTMPM
RGRADSLNAAVAGAVLAYEVLDTFENPAS

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=298, Percent_Identity=24.496644295302, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24666840, Length=275, Percent_Identity=24, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29349; Mature: 29218

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00496 PII_GLNB_UMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRQITGFSNPTVKYLRSLREKKHRKRERKFLAEGLRLLTDARECDRVPEMLVMAERRD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PHPLLTALEEAVTGAGGEVIETSDEILAKITGKSNPQAVAGVFAEWNTSLSALDRQSAPI
CCHHHHHHHHHHCCCCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCCCCH
WLVAQALRDPGNLGTMLRTGDAVGAGGLILLDDCVDPFSVEAVRASMGAVFTQQIAQARW
HHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
DDFLLWLRSGDGQLVAASLRDAVPYRGAAYAAPCFILVGNESQGLPENYETACDLRVTMP
HHEEEEEECCCCCEEEEHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECC
MRGRADSLNAAVAGAVLAYEVLDTFENPAS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRRQITGFSNPTVKYLRSLREKKHRKRERKFLAEGLRLLTDARECDRVPEMLVMAERRD
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PHPLLTALEEAVTGAGGEVIETSDEILAKITGKSNPQAVAGVFAEWNTSLSALDRQSAPI
CCHHHHHHHHHHCCCCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHCCCCCH
WLVAQALRDPGNLGTMLRTGDAVGAGGLILLDDCVDPFSVEAVRASMGAVFTQQIAQARW
HHHHHHHHCCCCCCHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
DDFLLWLRSGDGQLVAASLRDAVPYRGAAYAAPCFILVGNESQGLPENYETACDLRVTMP
HHEEEEEECCCCCEEEEHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEEEECC
MRGRADSLNAAVAGAVLAYEVLDTFENPAS
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]