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The map label for this gene is garR [C]

Identifier: 85373152

GI number: 85373152

Start: 366626

End: 367531

Strand: Reverse

Name: garR [C]

Synonym: ELI_01625

Alternate gene names: 85373152

Gene position: 367531-366626 (Counterclockwise)

Preceding gene: 85373157

Following gene: 85373151

Centisome position: 12.04

GC content: 65.67

Gene sequence:

>906_bases
ATGAGCGAGATCAAGACGATAGCTTTTCTCGGCCTCGGTGTGATGGGCGCACCGATGGCAGGGCACCTGGCGTGGGCAGG
CTATGCGGTGACGGGCTACAACCGCACCGCCTCGCGGGCCGCAGCCTGGGCCGATAAGCTCGGCGACGAAGGTCTCGAAG
TCGGGACAGCAGATACACCGGCAGAAGCCGCGCGAGGCAAGGATCTCGTCATGGCCTGCGTCGGAAACGACGAAGATCTG
GAACAGGTCCTGCTCGGCGAAACAGGCGCTCTGCCTGCCATGCGCGAAGGCTCGCTGCTGGTCGACCATACCACCGTTTC
GGCGAAAATGGCGCGGCGGATCGATGCTGCGGCCCGCGAACGCGGGGTCGCAGCGGTCGACGCCCCGGTGTCTGGGGGGC
AGGCCGGTGCCGAAAACGGCAAGCTCGCCGTGATGTGCGGTGGCAGCCAGGCGGCGGTCGAGCGCGCGCGTCCGGCGATG
GAGTCATTCGCGGCGCGCATCGTCCATGTCGGCGAAGCGGGCGCGGGCCAGACCACCAAGATGGCCAACCAGATGTGTAT
CGCCGGGGTCCTGGCGGGCCTGTCCGAAGCGATCCGGCTGTCGCAGGCTTCGGGCCTCGACATGGACAAGGTATATGACG
CCATCTCCGGCGGGGCGGCGCAAAGCTGGCAGATGGACAATCGCTGGGCGACGATGGCGAAGGGCGAATTCGATTTCGGC
TTCGCGATCGACTGGATGCGCAAAGACATGGGCTATGCGCTGGAAGAAGCCCAGCGTCTCGGCCTCAGTTCGCCGGTTTC
GGCGCTGGTTGACCAGTTCTACGCAGAGGTTCAGGCCATGGGCGGTGCGCGGCAGGATACCAGCGCCTTGATCCGTCGCC
TGCCTCAAGGAAAGAAAGACACCTGA

Upstream 100 bases:

>100_bases
CCCTCGTGTCCATGGCTGCGCGCCCTAGGGGCGATACACACCTCGCGCAATTCGAAAGAATGTTGGCAGCGGCAAACATT
GCCATTACGCGCATGGCCGC

Downstream 100 bases:

>100_bases
TGTTCAAGAAATCGCTGGCCGCGCTCGCGGCCCTTTCCCTCGCAACGCCTGCCGTTGCGGATGTGCTGGTCGACAATATC
GCCGGCATCCGCATCGACGA

Product: putative oxidoreductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSEIKTIAFLGLGVMGAPMAGHLAWAGYAVTGYNRTASRAAAWADKLGDEGLEVGTADTPAEAARGKDLVMACVGNDEDL
EQVLLGETGALPAMREGSLLVDHTTVSAKMARRIDAAARERGVAAVDAPVSGGQAGAENGKLAVMCGGSQAAVERARPAM
ESFAARIVHVGEAGAGQTTKMANQMCIAGVLAGLSEAIRLSQASGLDMDKVYDAISGGAAQSWQMDNRWATMAKGEFDFG
FAIDWMRKDMGYALEEAQRLGLSSPVSALVDQFYAEVQAMGGARQDTSALIRRLPQGKKDT

Sequences:

>Translated_301_residues
MSEIKTIAFLGLGVMGAPMAGHLAWAGYAVTGYNRTASRAAAWADKLGDEGLEVGTADTPAEAARGKDLVMACVGNDEDL
EQVLLGETGALPAMREGSLLVDHTTVSAKMARRIDAAARERGVAAVDAPVSGGQAGAENGKLAVMCGGSQAAVERARPAM
ESFAARIVHVGEAGAGQTTKMANQMCIAGVLAGLSEAIRLSQASGLDMDKVYDAISGGAAQSWQMDNRWATMAKGEFDFG
FAIDWMRKDMGYALEEAQRLGLSSPVSALVDQFYAEVQAMGGARQDTSALIRRLPQGKKDT
>Mature_300_residues
SEIKTIAFLGLGVMGAPMAGHLAWAGYAVTGYNRTASRAAAWADKLGDEGLEVGTADTPAEAARGKDLVMACVGNDEDLE
QVLLGETGALPAMREGSLLVDHTTVSAKMARRIDAAARERGVAAVDAPVSGGQAGAENGKLAVMCGGSQAAVERARPAME
SFAARIVHVGEAGAGQTTKMANQMCIAGVLAGLSEAIRLSQASGLDMDKVYDAISGGAAQSWQMDNRWATMAKGEFDFGF
AIDWMRKDMGYALEEAQRLGLSSPVSALVDQFYAEVQAMGGARQDTSALIRRLPQGKKDT

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=27.4305555555556, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=26.7796610169492, Blast_Score=111, Evalue=8e-25,
Organism=Escherichia coli, GI145693186, Length=284, Percent_Identity=35.5633802816901, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1786719, Length=290, Percent_Identity=34.1379310344828, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI1790315, Length=289, Percent_Identity=29.0657439446367, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789092, Length=288, Percent_Identity=30.2083333333333, Blast_Score=120, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17557316, Length=274, Percent_Identity=25.1824817518248, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574115, Length=290, Percent_Identity=23.448275862069, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24655230, Length=301, Percent_Identity=27.906976744186, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922568, Length=301, Percent_Identity=27.906976744186, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31391; Mature: 31260

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIKTIAFLGLGVMGAPMAGHLAWAGYAVTGYNRTASRAAAWADKLGDEGLEVGTADTP
CCCHHHHHHHHHHHHCCCCHHHHHHHCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
AEAARGKDLVMACVGNDEDLEQVLLGETGALPAMREGSLLVDHTTVSAKMARRIDAAARE
HHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
RGVAAVDAPVSGGQAGAENGKLAVMCGGSQAAVERARPAMESFAARIVHVGEAGAGQTTK
CCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
MANQMCIAGVLAGLSEAIRLSQASGLDMDKVYDAISGGAAQSWQMDNRWATMAKGEFDFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
FAIDWMRKDMGYALEEAQRLGLSSPVSALVDQFYAEVQAMGGARQDTSALIRRLPQGKKD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
T
C
>Mature Secondary Structure 
SEIKTIAFLGLGVMGAPMAGHLAWAGYAVTGYNRTASRAAAWADKLGDEGLEVGTADTP
CCHHHHHHHHHHHHCCCCHHHHHHHCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCC
AEAARGKDLVMACVGNDEDLEQVLLGETGALPAMREGSLLVDHTTVSAKMARRIDAAARE
HHHHCCCCEEEEECCCCHHHHHHHHCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHH
RGVAAVDAPVSGGQAGAENGKLAVMCGGSQAAVERARPAMESFAARIVHVGEAGAGQTTK
CCCEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
MANQMCIAGVLAGLSEAIRLSQASGLDMDKVYDAISGGAAQSWQMDNRWATMAKGEFDFG
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCC
FAIDWMRKDMGYALEEAQRLGLSSPVSALVDQFYAEVQAMGGARQDTSALIRRLPQGKKD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
T
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA