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The map label for this gene is ispH [H]

Identifier: 85373139

GI number: 85373139

Start: 355682

End: 356659

Strand: Reverse

Name: ispH [H]

Synonym: ELI_01560

Alternate gene names: 85373139

Gene position: 356659-355682 (Counterclockwise)

Preceding gene: 85373149

Following gene: 85373138

Centisome position: 11.68

GC content: 60.94

Gene sequence:

>978_bases
ATGAACGCTCCCTTTCAATCCCCCGAGCCCTCGCAGTCCGGCAAACCCGGCTTGAGCCTCCTGATTGCGGCTCCGCGTGG
CTTTTGTGCCGGTGTCGACCGCGCCATCGACATCGTCGAACGCGCCCTCGATAAATACGGTGCGCCGGTCTATGTCCGGC
ATGAGATCGTGCACAACAAGTACGTGGTCGACGCTTTGCGTGAAAAAGGGGCCATCTTCGTCGAAGAGCTCGACGAGGTG
CCGGACGGTGTCCCCGTGGTGTTCAGTGCGCACGGTGTGCCGAAGTCGGTGCCGGCCGAAGCCGACCGGCGGCAATTGCT
CTATGTCGATGCGACCTGCCCGCTCGTAAGCAAGGTTCATCGTCAGGCCGAGAGACAGATCGAGAAAGACCGACATATCG
TGTTTATCGGCCACGCCGGGCATCCTGAAGTGGTGGGTACGATGGGGCAGGTCGAAGCCGGGCAGATGACGCTGGTTGAA
ACCGTGGAGGATGTTGCCGCACTGCCGTTTGGCGCGGATGACAAGCTCGCCTACCTCACGCAGACCACACTGTCGGTCGA
CGACACGGCGGAAATCATCGCTGCGCTCGAGGCGCGCTATCCCGAGATCGCAGGACCGAAAGCGGAGGATATCTGCTACG
CCACATCGAACCGACAGGCTGCCGTCAAACGGATTGCCCCCGGTAGCGACCTGGTGCTGGTCATCGGCGCGACCAATTCC
TCGAATTCGCTGCGACTGGTCGAGGTTGCAGAAAGGCTCGGGACGAAGGCCAAGCTGATCCAGCGTGCAGACGATATCGA
TCCCGCTTGGCTCGAAGGAGTCGGCACGATCGGTCTCACCGCCGGCGCTTCCGCGCCCGAACTGCTTGTTCGCGAAGTGG
TCGAGAAACTCGGCGAGTGGCGCGAAGTCCAAGAGAACACGATTGCTGCCGCCGAGGAAAATATGGTCTTCAAATTGCCG
CGCCAATTGATGGATTGA

Upstream 100 bases:

>100_bases
GGGCAAGGGTCGGGCGGAAATTGCAGTTCATCGAAGTCCTCACGTCAATCGCTCACGCGTCCCCTAGCGGGCGTCTTTCG
AAAGCGCTAGAGGGCGGGCT

Downstream 100 bases:

>100_bases
GCACGCCACATGGCGGTTTACACCCATCTTTCGGCAGAAGATCTGGCCCGCCTGATCGGGCACTACGATGTCGGAAAACT
GGTTTCGGCGAAGGGAATTG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MNAPFQSPEPSQSGKPGLSLLIAAPRGFCAGVDRAIDIVERALDKYGAPVYVRHEIVHNKYVVDALREKGAIFVEELDEV
PDGVPVVFSAHGVPKSVPAEADRRQLLYVDATCPLVSKVHRQAERQIEKDRHIVFIGHAGHPEVVGTMGQVEAGQMTLVE
TVEDVAALPFGADDKLAYLTQTTLSVDDTAEIIAALEARYPEIAGPKAEDICYATSNRQAAVKRIAPGSDLVLVIGATNS
SNSLRLVEVAERLGTKAKLIQRADDIDPAWLEGVGTIGLTAGASAPELLVREVVEKLGEWREVQENTIAAAEENMVFKLP
RQLMD

Sequences:

>Translated_325_residues
MNAPFQSPEPSQSGKPGLSLLIAAPRGFCAGVDRAIDIVERALDKYGAPVYVRHEIVHNKYVVDALREKGAIFVEELDEV
PDGVPVVFSAHGVPKSVPAEADRRQLLYVDATCPLVSKVHRQAERQIEKDRHIVFIGHAGHPEVVGTMGQVEAGQMTLVE
TVEDVAALPFGADDKLAYLTQTTLSVDDTAEIIAALEARYPEIAGPKAEDICYATSNRQAAVKRIAPGSDLVLVIGATNS
SNSLRLVEVAERLGTKAKLIQRADDIDPAWLEGVGTIGLTAGASAPELLVREVVEKLGEWREVQENTIAAAEENMVFKLP
RQLMD
>Mature_325_residues
MNAPFQSPEPSQSGKPGLSLLIAAPRGFCAGVDRAIDIVERALDKYGAPVYVRHEIVHNKYVVDALREKGAIFVEELDEV
PDGVPVVFSAHGVPKSVPAEADRRQLLYVDATCPLVSKVHRQAERQIEKDRHIVFIGHAGHPEVVGTMGQVEAGQMTLVE
TVEDVAALPFGADDKLAYLTQTTLSVDDTAEIIAALEARYPEIAGPKAEDICYATSNRQAAVKRIAPGSDLVLVIGATNS
SNSLRLVEVAERLGTKAKLIQRADDIDPAWLEGVGTIGLTAGASAPELLVREVVEKLGEWREVQENTIAAAEENMVFKLP
RQLMD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=308, Percent_Identity=54.2207792207792, Blast_Score=344, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 35113; Mature: 35113

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPFQSPEPSQSGKPGLSLLIAAPRGFCAGVDRAIDIVERALDKYGAPVYVRHEIVHNK
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
YVVDALREKGAIFVEELDEVPDGVPVVFSAHGVPKSVPAEADRRQLLYVDATCPLVSKVH
HHHHHHHHCCCEEHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
RQAERQIEKDRHIVFIGHAGHPEVVGTMGQVEAGQMTLVETVEDVAALPFGADDKLAYLT
HHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
QTTLSVDDTAEIIAALEARYPEIAGPKAEDICYATSNRQAAVKRIAPGSDLVLVIGATNS
EEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCC
SNSLRLVEVAERLGTKAKLIQRADDIDPAWLEGVGTIGLTAGASAPELLVREVVEKLGEW
CCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHH
REVQENTIAAAEENMVFKLPRQLMD
HHHHHHHHHHHHCCHHHCCHHHHCC
>Mature Secondary Structure
MNAPFQSPEPSQSGKPGLSLLIAAPRGFCAGVDRAIDIVERALDKYGAPVYVRHEIVHNK
CCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
YVVDALREKGAIFVEELDEVPDGVPVVFSAHGVPKSVPAEADRRQLLYVDATCPLVSKVH
HHHHHHHHCCCEEHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHH
RQAERQIEKDRHIVFIGHAGHPEVVGTMGQVEAGQMTLVETVEDVAALPFGADDKLAYLT
HHHHHHHHCCCCEEEEECCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEE
QTTLSVDDTAEIIAALEARYPEIAGPKAEDICYATSNRQAAVKRIAPGSDLVLVIGATNS
EEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHCCCCCCEEEEEECCCC
SNSLRLVEVAERLGTKAKLIQRADDIDPAWLEGVGTIGLTAGASAPELLVREVVEKLGEW
CCCCHHHHHHHHHCCHHHHHHHHCCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHH
REVQENTIAAAEENMVFKLPRQLMD
HHHHHHHHHHHHCCHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA