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The map label for this gene is 85373112

Identifier: 85373112

GI number: 85373112

Start: 319908

End: 322139

Strand: Reverse

Name: 85373112

Synonym: ELI_01425

Alternate gene names: NA

Gene position: 322139-319908 (Counterclockwise)

Preceding gene: 85373117

Following gene: 85373111

Centisome position: 10.55

GC content: 65.1

Gene sequence:

>2232_bases
ATGCTGCGGATCGGCGGCGGCGTGCTGGCGCTCGTGCTGGTGGTGCTTGTAGGGCTGATGACGTGGGAGCCGTTCTTCGC
CCGCGAGACCGCCGCGCCCAGCACGCACCGTACGTACCGGGCCGAAATTCTCCGCAGCGACTACGGCGTGCCGCATATCT
ACGGCGAGACCGATGCCGATGTCGCTTACGGCGTGGCCATCGCGCAGAGCGAGGACGATTTTTTCACGCTGCAGGACGTG
GTCGCCATGTCCAAGGGCCGCTATGGCGCGATCGCCGGGCCCGACGGCGCGCAGGTCGATTACGTCTATTACCTGCTCGA
CGCGCGCGGCACGGCCGAACGGCAGTATGGCAGCCTGCCCGCCGATACGCGCGCCATCTTCGATGCTTATGCCACCGGGC
TCAACCAATACGCCGAAGAGAATCCCGACGAGCTGAAACTCGCCGGGCTGTTCCCGGTCAATGGCGAGGATGTCGCCGCG
GGCTTTGCGCTGCGTCAGCCTTTCTTCTTCGGGCTTGCCGGAGTCATCGGTCAACTCTCCGACAACAAGCCGCCGCGGCG
CGAGTTCGGCCCCGATATTCCCGGCTTCCCGCGCGAAGGCAGCGGGGGCGACGAGATCGAAGTGGAGCGCAAACCTGCGC
AGACCGCCCGTGCCATGCCGCTTCCGTGGGGTGAAATGGCGGCGCTTTCAGGCTCCAATGCCTTTGCCGTCGCGCCTGAA
AAGTCGGGGGAAGGCACGATCCTGATCTCCAACAGCCACCAGCCGCTGGAAGGGGGCGTCGCGTGGTACGAAATGGTCGT
CGAGAGCGGCGAAGGGTGGCATTTTACCGGGGCCAACTTCCCCGGCTCGCCCTTCCCCTTCCTCGGGCACAATCGCACGC
TCGGCTGGACCAACACCGTCAATCGCCCGGACATGGTCGATATCTACGAGCTGGTGCTGAACGAGGACGGTACCCAATAC
CGGCTCGACGGCGAATGGCGTGATCTCGAAGCGCGCGAGGTCACGCTGCCGGTAAAGCTCGGCCCGCTTGTGCTGCCGAT
CCGCGAAACCGTCTATCGCAGCGTCCACGGCCCGGTGATCGTGAACGACAACGGCGCCTTCGCTATGCGCTATGGCGGGA
TCGACAATATCGACCAGCTCGACGCCTATTACCGGCTCAACAAGGCCAGCACGCTGGAGGAATGGCAGGCGATCCTCTCC
CGGATGGACGTGCCCTCCACCAATTTCATCTATGGCGATGCCGAGGGCAATATCGCCATGGTCTACAACGCAGCGATCCC
GGACCGCCCGGAAGGGCACGACTGGCGCGGCATCCTGCCCGGCGATCGCTCAGACCTGATCTGGGACGGTCCGGTCGACT
ACGCCGCAATCCCCAAATACGTGAACCCGGCCAGCGGCTGGCTGATGAATGCCAACAACACGCCGTTCAGGGCGGCAGGC
CCGGACAGCGACCTCTCGCCCGACAGCGTTTCGCCCTATCTCGGCGTCGAGCTGAAGCAGACCAACCGGTCGCGCCGCGC
GTGGAAGCTGATGAGCGAGGCCGGGCGGCTCGACAACGACACCCTGCGGCGGATCAAATACGACACCGCCTACGAACGCG
AAGGCTATGTCGCAGATCTGTGGGACGCGCTCGAACAATTCGAGCCGGGCGATGCCGATCTCGGCGAAGCGCGCCGCATC
CTGCTGGAATGGGATTTCACCGCCGACAATGTCGGCCCGGGCGACGCCCTGGCGCTGCTGATGATTAGGGACTTCATGTC
GGCCGAATACCAGAACAAAGCCGAAGCGCCCGATGTCGCCACACATCTGCGCAAGGCGGTCGATCACCTGCAGGAGCACT
TCGGTCGGCTCGATCCGCCGATGAGCGAGCTGCTGCGCCTGCGGCAAGACCCGGGGCTGCATGCCGTCGACCTGCCGCTC
GACGGCGGTTCGGACACGCTGCGCGCATCGACCACCTGGCAGGTCGATCCCGACGGCCGCCTGCGGCTGGTCCATGGCGA
CAGCTTCATCCAGTGGGTCGAGTGGCGACCGGGCGAGCGGGTGCGATCGCGCTCGGTCCAGCCGTTCGGTGCAGCGATTA
CCCGGCCCGACAGCCCGCATTTCACCGATCAGGCGACGCTGTTCGTCCAGAAGAAGCTGAAACCGGTCCACTTCTGGCGC
GAAGATGCGCTCGCCAATGCCCGGAGCCGCAAAATCGTCACGAACCAAAAGGCAGTGACCAGCCGGAACTGA

Upstream 100 bases:

>100_bases
GCCCCGCGACTTATCCTGAGTCGATTTGCCCCTTCACCCCGCTCACGCGGGTCGCTAGGCTGGCCACAACCGGAGAGGTC
TGACTACGATGAGAAAATGG

Downstream 100 bases:

>100_bases
CTCGATACATACCCTTTTCAATAAAGAACCACACCTGTCCTGGAGAGACCATGCTTCGTTTTGCTTCGACCAGCCTAGCC
GCCATCGCAATCGCGACGTC

Product: penicillin amidase family protein

Products: NA

Alternate protein names: Glutaryl-7-ACA acylase; 7-beta-(4-carboxybutanamido)cephalosporanic acid acylase; CAD; GL-7-ACA acylase; GCA; Glutaryl-7-ACA acylase subunit alpha; Glutaryl-7-ACA acylase subunit beta [H]

Number of amino acids: Translated: 743; Mature: 743

Protein sequence:

>743_residues
MLRIGGGVLALVLVVLVGLMTWEPFFARETAAPSTHRTYRAEILRSDYGVPHIYGETDADVAYGVAIAQSEDDFFTLQDV
VAMSKGRYGAIAGPDGAQVDYVYYLLDARGTAERQYGSLPADTRAIFDAYATGLNQYAEENPDELKLAGLFPVNGEDVAA
GFALRQPFFFGLAGVIGQLSDNKPPRREFGPDIPGFPREGSGGDEIEVERKPAQTARAMPLPWGEMAALSGSNAFAVAPE
KSGEGTILISNSHQPLEGGVAWYEMVVESGEGWHFTGANFPGSPFPFLGHNRTLGWTNTVNRPDMVDIYELVLNEDGTQY
RLDGEWRDLEAREVTLPVKLGPLVLPIRETVYRSVHGPVIVNDNGAFAMRYGGIDNIDQLDAYYRLNKASTLEEWQAILS
RMDVPSTNFIYGDAEGNIAMVYNAAIPDRPEGHDWRGILPGDRSDLIWDGPVDYAAIPKYVNPASGWLMNANNTPFRAAG
PDSDLSPDSVSPYLGVELKQTNRSRRAWKLMSEAGRLDNDTLRRIKYDTAYEREGYVADLWDALEQFEPGDADLGEARRI
LLEWDFTADNVGPGDALALLMIRDFMSAEYQNKAEAPDVATHLRKAVDHLQEHFGRLDPPMSELLRLRQDPGLHAVDLPL
DGGSDTLRASTTWQVDPDGRLRLVHGDSFIQWVEWRPGERVRSRSVQPFGAAITRPDSPHFTDQATLFVQKKLKPVHFWR
EDALANARSRKIVTNQKAVTSRN

Sequences:

>Translated_743_residues
MLRIGGGVLALVLVVLVGLMTWEPFFARETAAPSTHRTYRAEILRSDYGVPHIYGETDADVAYGVAIAQSEDDFFTLQDV
VAMSKGRYGAIAGPDGAQVDYVYYLLDARGTAERQYGSLPADTRAIFDAYATGLNQYAEENPDELKLAGLFPVNGEDVAA
GFALRQPFFFGLAGVIGQLSDNKPPRREFGPDIPGFPREGSGGDEIEVERKPAQTARAMPLPWGEMAALSGSNAFAVAPE
KSGEGTILISNSHQPLEGGVAWYEMVVESGEGWHFTGANFPGSPFPFLGHNRTLGWTNTVNRPDMVDIYELVLNEDGTQY
RLDGEWRDLEAREVTLPVKLGPLVLPIRETVYRSVHGPVIVNDNGAFAMRYGGIDNIDQLDAYYRLNKASTLEEWQAILS
RMDVPSTNFIYGDAEGNIAMVYNAAIPDRPEGHDWRGILPGDRSDLIWDGPVDYAAIPKYVNPASGWLMNANNTPFRAAG
PDSDLSPDSVSPYLGVELKQTNRSRRAWKLMSEAGRLDNDTLRRIKYDTAYEREGYVADLWDALEQFEPGDADLGEARRI
LLEWDFTADNVGPGDALALLMIRDFMSAEYQNKAEAPDVATHLRKAVDHLQEHFGRLDPPMSELLRLRQDPGLHAVDLPL
DGGSDTLRASTTWQVDPDGRLRLVHGDSFIQWVEWRPGERVRSRSVQPFGAAITRPDSPHFTDQATLFVQKKLKPVHFWR
EDALANARSRKIVTNQKAVTSRN
>Mature_743_residues
MLRIGGGVLALVLVVLVGLMTWEPFFARETAAPSTHRTYRAEILRSDYGVPHIYGETDADVAYGVAIAQSEDDFFTLQDV
VAMSKGRYGAIAGPDGAQVDYVYYLLDARGTAERQYGSLPADTRAIFDAYATGLNQYAEENPDELKLAGLFPVNGEDVAA
GFALRQPFFFGLAGVIGQLSDNKPPRREFGPDIPGFPREGSGGDEIEVERKPAQTARAMPLPWGEMAALSGSNAFAVAPE
KSGEGTILISNSHQPLEGGVAWYEMVVESGEGWHFTGANFPGSPFPFLGHNRTLGWTNTVNRPDMVDIYELVLNEDGTQY
RLDGEWRDLEAREVTLPVKLGPLVLPIRETVYRSVHGPVIVNDNGAFAMRYGGIDNIDQLDAYYRLNKASTLEEWQAILS
RMDVPSTNFIYGDAEGNIAMVYNAAIPDRPEGHDWRGILPGDRSDLIWDGPVDYAAIPKYVNPASGWLMNANNTPFRAAG
PDSDLSPDSVSPYLGVELKQTNRSRRAWKLMSEAGRLDNDTLRRIKYDTAYEREGYVADLWDALEQFEPGDADLGEARRI
LLEWDFTADNVGPGDALALLMIRDFMSAEYQNKAEAPDVATHLRKAVDHLQEHFGRLDPPMSELLRLRQDPGLHAVDLPL
DGGSDTLRASTTWQVDPDGRLRLVHGDSFIQWVEWRPGERVRSRSVQPFGAAITRPDSPHFTDQATLFVQKKLKPVHFWR
EDALANARSRKIVTNQKAVTSRN

Specific function: Catalyzes the deacylation of 7 beta-(4- carboxybutanamido)cephalosporanic acid (glutaryl-7- aminocephalosporanic acid or GL-7-ACA) to 7-aminocephalosporanic acid (7-ACA). Can not efficiently use cephalosporin C (CPC), penicillin G, or ampicillin as substr

COG id: COG2366

COG function: function code R; Protein related to penicillin acylase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S45 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014395
- InterPro:   IPR002692 [H]

Pfam domain/function: PF01804 Penicil_amidase [H]

EC number: =3.5.1.93 [H]

Molecular weight: Translated: 82388; Mature: 82388

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIGGGVLALVLVVLVGLMTWEPFFARETAAPSTHRTYRAEILRSDYGVPHIYGETDAD
CEEECCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCC
VAYGVAIAQSEDDFFTLQDVVAMSKGRYGAIAGPDGAQVDYVYYLLDARGTAERQYGSLP
CEEEEEEECCCCCCCHHHHHHHHCCCCCCEEECCCCCCEEEEEEEEECCCCCHHHCCCCC
ADTRAIFDAYATGLNQYAEENPDELKLAGLFPVNGEDVAAGFALRQPFFFGLAGVIGQLS
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCHHHHCHHHHHHHHHHHHHC
DNKPPRREFGPDIPGFPREGSGGDEIEVERKPAQTARAMPLPWGEMAALSGSNAFAVAPE
CCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCCHHHEECCCCEEEECCC
KSGEGTILISNSHQPLEGGVAWYEMVVESGEGWHFTGANFPGSPFPFLGHNRTLGWTNTV
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
NRPDMVDIYELVLNEDGTQYRLDGEWRDLEAREVTLPVKLGPLVLPIRETVYRSVHGPVI
CCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEHHHHHHHHHCCCCEE
VNDNGAFAMRYGGIDNIDQLDAYYRLNKASTLEEWQAILSRMDVPSTNFIYGDAEGNIAM
ECCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEE
VYNAAIPDRPEGHDWRGILPGDRSDLIWDGPVDYAAIPKYVNPASGWLMNANNTPFRAAG
EEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCCCCCEEECCCCCCCCCCC
PDSDLSPDSVSPYLGVELKQTNRSRRAWKLMSEAGRLDNDTLRRIKYDTAYEREGYVADL
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCCHHHH
WDALEQFEPGDADLGEARRILLEWDFTADNVGPGDALALLMIRDFMSAEYQNKAEAPDVA
HHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
THLRKAVDHLQEHFGRLDPPMSELLRLRQDPGLHAVDLPLDGGSDTLRASTTWQVDPDGR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCEEECEEEEECCCCC
LRLVHGDSFIQWVEWRPGERVRSRSVQPFGAAITRPDSPHFTDQATLFVQKKLKPVHFWR
EEEEECCCEEEEECCCCCHHHHHCCCCCCCCEEECCCCCCCCCHHHEEEHHHCCHHHHHH
EDALANARSRKIVTNQKAVTSRN
HHHHHCCCCCEEEECCCHHCCCC
>Mature Secondary Structure
MLRIGGGVLALVLVVLVGLMTWEPFFARETAAPSTHRTYRAEILRSDYGVPHIYGETDAD
CEEECCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCC
VAYGVAIAQSEDDFFTLQDVVAMSKGRYGAIAGPDGAQVDYVYYLLDARGTAERQYGSLP
CEEEEEEECCCCCCCHHHHHHHHCCCCCCEEECCCCCCEEEEEEEEECCCCCHHHCCCCC
ADTRAIFDAYATGLNQYAEENPDELKLAGLFPVNGEDVAAGFALRQPFFFGLAGVIGQLS
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHCCHHHHCHHHHHHHHHHHHHC
DNKPPRREFGPDIPGFPREGSGGDEIEVERKPAQTARAMPLPWGEMAALSGSNAFAVAPE
CCCCCHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHCCCCCCCCHHHEECCCCEEEECCC
KSGEGTILISNSHQPLEGGVAWYEMVVESGEGWHFTGANFPGSPFPFLGHNRTLGWTNTV
CCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCC
NRPDMVDIYELVLNEDGTQYRLDGEWRDLEAREVTLPVKLGPLVLPIRETVYRSVHGPVI
CCCCHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEEEEEECCEEEHHHHHHHHHCCCCEE
VNDNGAFAMRYGGIDNIDQLDAYYRLNKASTLEEWQAILSRMDVPSTNFIYGDAEGNIAM
ECCCCEEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCEEE
VYNAAIPDRPEGHDWRGILPGDRSDLIWDGPVDYAAIPKYVNPASGWLMNANNTPFRAAG
EEECCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCCCCCCCCCEEECCCCCCCCCCC
PDSDLSPDSVSPYLGVELKQTNRSRRAWKLMSEAGRLDNDTLRRIKYDTAYEREGYVADL
CCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCCHHHH
WDALEQFEPGDADLGEARRILLEWDFTADNVGPGDALALLMIRDFMSAEYQNKAEAPDVA
HHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
THLRKAVDHLQEHFGRLDPPMSELLRLRQDPGLHAVDLPLDGGSDTLRASTTWQVDPDGR
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCEEECEEEEECCCCC
LRLVHGDSFIQWVEWRPGERVRSRSVQPFGAAITRPDSPHFTDQATLFVQKKLKPVHFWR
EEEEECCCEEEEECCCCCHHHHHCCCCCCCCEEECCCCCCCCCHHHEEEHHHCCHHHHHH
EDALANARSRKIVTNQKAVTSRN
HHHHHCCCCCEEEECCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11080627 [H]