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The map label for this gene is valS [H]

Identifier: 85373085

GI number: 85373085

Start: 296980

End: 297564

Strand: Reverse

Name: valS [H]

Synonym: ELI_01290

Alternate gene names: 85373085

Gene position: 297564-296980 (Counterclockwise)

Preceding gene: 85373086

Following gene: 85373084

Centisome position: 9.75

GC content: 65.3

Gene sequence:

>585_bases
ATGCCCTTCATCACCGAGGAGCTGTGGCATGCCCAAGGAACAACAAGCGGGCGTGAGCGGCCCTACGAACTGATCCTCGC
GCAATGGCCAAACCCGCGCGCGGAAGTTTCCAAGGAAGCGACCGATGCGATCGACTGGGTGATCGACCTCACGACCAATG
TCCGCTCGGCCAAGAACGAGCTCGGCATCCCGCCGGGCGAGAAGCTGGCGGCCTTCGTGCCCTCGCCTTCGGACTTGGCC
GCCAGCACGATCGAGCGCAGCAGCGCCGCCATCGAACGGCTCGCGCGGTTGACGCCCGTGACAGTCGGCGAGGCCCCGTC
CGGCCCCGCGATGCAGGTGACTGCGCTCTCGGACGTGTTCGTTATCCCGCTCGAAGGCATAATCGATATCGAGGCCGAGA
AAACGCGCCTCAGCAAGGCCCTCGAAGCCTCCCAGAAGGAAGTGAAATCGCTCGACGGGCGGCTGTCCAACCCCCACTTC
GTGGAGCGTGCCAAGCCCGAAGCGGTCGAGAAAGCCAAGGCCGACCACGCGCATCACAGCGCCGAAGTCGAGCGGTTGCA
GACAGCGCTCGCACGGTTGGGGTAG

Upstream 100 bases:

>100_bases
CCCCCTCCCCTGAAGGAGAGGGGAGTGGTCGCCCGCCACCGAAACGCGCCAAGTCGCCGGCTGGGCGCTCGACCAAATCC
TCGTCATGCTACACCCCTTC

Downstream 100 bases:

>100_bases
ACCTACCCAAACCGCCGGAGCGACCTTACCTTCACCTGATGTCGCCTTGGCTTGCCCTCTCGATACCGCTGCTGATCGTC
GCAGCCCTGCTGTACCGGCG

Product: valyl-tRNA synthetase

Products: NA

Alternate protein names: Valine--tRNA ligase; ValRS [H]

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MPFITEELWHAQGTTSGRERPYELILAQWPNPRAEVSKEATDAIDWVIDLTTNVRSAKNELGIPPGEKLAAFVPSPSDLA
ASTIERSSAAIERLARLTPVTVGEAPSGPAMQVTALSDVFVIPLEGIIDIEAEKTRLSKALEASQKEVKSLDGRLSNPHF
VERAKPEAVEKAKADHAHHSAEVERLQTALARLG

Sequences:

>Translated_194_residues
MPFITEELWHAQGTTSGRERPYELILAQWPNPRAEVSKEATDAIDWVIDLTTNVRSAKNELGIPPGEKLAAFVPSPSDLA
ASTIERSSAAIERLARLTPVTVGEAPSGPAMQVTALSDVFVIPLEGIIDIEAEKTRLSKALEASQKEVKSLDGRLSNPHF
VERAKPEAVEKAKADHAHHSAEVERLQTALARLG
>Mature_193_residues
PFITEELWHAQGTTSGRERPYELILAQWPNPRAEVSKEATDAIDWVIDLTTNVRSAKNELGIPPGEKLAAFVPSPSDLAA
STIERSSAAIERLARLTPVTVGEAPSGPAMQVTALSDVFVIPLEGIIDIEAEKTRLSKALEASQKEVKSLDGRLSNPHFV
ERAKPEAVEKAKADHAHHSAEVERLQTALARLG

Specific function: Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val)

COG id: COG0525

COG function: function code J; Valyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790708, Length=174, Percent_Identity=30.4597701149425, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR014729
- InterPro:   IPR010978
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR019499
- InterPro:   IPR009008
- InterPro:   IPR002303 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; PF10458 Val_tRNA-synt_C [H]

EC number: =6.1.1.9 [H]

Molecular weight: Translated: 21044; Mature: 20913

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFITEELWHAQGTTSGRERPYELILAQWPNPRAEVSKEATDAIDWVIDLTTNVRSAKNE
CCCHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIPPGEKLAAFVPSPSDLAASTIERSSAAIERLARLTPVTVGEAPSGPAMQVTALSDVF
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEE
VIPLEGIIDIEAEKTRLSKALEASQKEVKSLDGRLSNPHFVERAKPEAVEKAKADHAHHS
EEECCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
AEVERLQTALARLG
HHHHHHHHHHHHCC
>Mature Secondary Structure 
PFITEELWHAQGTTSGRERPYELILAQWPNPRAEVSKEATDAIDWVIDLTTNVRSAKNE
CCHHHHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGIPPGEKLAAFVPSPSDLAASTIERSSAAIERLARLTPVTVGEAPSGPAMQVTALSDVF
CCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCEE
VIPLEGIIDIEAEKTRLSKALEASQKEVKSLDGRLSNPHFVERAKPEAVEKAKADHAHHS
EEECCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHH
AEVERLQTALARLG
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA