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The map label for this gene is nhaA1

Identifier: 85373075

GI number: 85373075

Start: 287462

End: 288679

Strand: Reverse

Name: nhaA1

Synonym: ELI_01240

Alternate gene names: 85373075

Gene position: 288679-287462 (Counterclockwise)

Preceding gene: 85373083

Following gene: 85373073

Centisome position: 9.46

GC content: 63.46

Gene sequence:

>1218_bases
ATGGTTGACCCGATCCGCAGCCCCCTGTCCCGCGCCTTTGCACCCGTCCGCGCCCTGTTTGTCAGCGATGCCTCTGCCGG
CATTCTGCTGATACTCGTGGCAGCGGCGGCCATGATCGTCGCCAATTCGCCGCTGGCGGGCGCTTACGAAGAAATGTTCT
ACGGCGATCTGGCATGGACCCCGATCGCCAAGCTCGACGATCTGCACCTGTGGATCAACGACGGCCTGATGGCGATTTTC
TTCTTCGTCGTCGGCCTGGAAGTGAAACGCGAGCTTATTTGCGGACAGCTATCCAGTCCCGAGCAACGCACGCTGCCGGT
GCTCGCGGCGATCGCAGGGATGGCCGTACCTGCGATTGTATATGTCGGCGTTGTCGGCACGGACAGCGCGCTGGTGCGCG
GCTGGGCGATCCCGGCCGCCACCGACATTGCCTTCGCCATGGGGGTGCTCGGCCTGCTCGGCAGTCGTGTGCCAGCCTCG
CTGCGGTTGTTCCTGCTGACTGTCGCCATCGTCGACGATATCGGTGCGGTGCTCGTAATCGCCGCCTTCTACACCGCGAA
CCTCAAGGTCATGTGGCTCGTCATCGCGCTGGGCATCTTCGGCGTCATGGTCGGCATGAACAAGTTCGGGGTCGACCGGA
TCTGGCCCTATATCCTCGTCGCGCTGGTGCTTTGGGTGGCGGTGCTGTTCTCGGGCGTTCATGCGACCATCGCCGGCGTG
ATGGCGGCACTTACCATCCCGATGCGCCGCAAGGACGGACACTCGCTTCTGGAGAAGCTCGAGCATGGCCTCGCGCCGTG
GAGCGCCTATCTTGTGGTGCCGATCTTCGGTTTCGCCAATGCCGGTGTGAACCTCAGCGGCATGGGTCTGGACGCTGTCC
TGGCCCCACTGCCGCTGGCCATCGCAGCAGGCCTGGTGGTGGGCAAACAGCTTGGCATTTTCGGCATCATCGTGGCTGCC
GTGAAACTCGGCATTGCCAAAGCACCGGCCAACGCCAACTGGATCGAGATCTGGGGTGTCAGCATCCTGACCGGGATCGG
CTTCACCATGTCGCTCTTCATCAGCGGGCTCGCCTTTACCGACAGCCGCCTGCTGATCGACGAGGCCAAGATCGGCATTC
TCGGCGGCTCGCTGATCTCGGCGATCCTCGGCTACACCATCCTGCGCCTGACGACGACGCATCCCGAAGAGCGACCGCCC
CAGACAGTCACCCCCTGA

Upstream 100 bases:

>100_bases
GAGATTCACGAGCGCTCCTGACTCTGTCCTCGTTAACTGACCTAGCGAACCGGATCGGGCCTTGCAACGCGGGAAATATC
CATCGATAGGGCGCGCACCT

Downstream 100 bases:

>100_bases
CGGAGCGCGAGCGGCTCACCAGCTCCCGGTATTCTCCATGCTGGCCCAGGGTTCCTGCGGCGGCAGATTGCCTTCCTGCA
GCAATTCGACCGATATTCCG

Product: Na+/H+ antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA 1

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MVDPIRSPLSRAFAPVRALFVSDASAGILLILVAAAAMIVANSPLAGAYEEMFYGDLAWTPIAKLDDLHLWINDGLMAIF
FFVVGLEVKRELICGQLSSPEQRTLPVLAAIAGMAVPAIVYVGVVGTDSALVRGWAIPAATDIAFAMGVLGLLGSRVPAS
LRLFLLTVAIVDDIGAVLVIAAFYTANLKVMWLVIALGIFGVMVGMNKFGVDRIWPYILVALVLWVAVLFSGVHATIAGV
MAALTIPMRRKDGHSLLEKLEHGLAPWSAYLVVPIFGFANAGVNLSGMGLDAVLAPLPLAIAAGLVVGKQLGIFGIIVAA
VKLGIAKAPANANWIEIWGVSILTGIGFTMSLFISGLAFTDSRLLIDEAKIGILGGSLISAILGYTILRLTTTHPEERPP
QTVTP

Sequences:

>Translated_405_residues
MVDPIRSPLSRAFAPVRALFVSDASAGILLILVAAAAMIVANSPLAGAYEEMFYGDLAWTPIAKLDDLHLWINDGLMAIF
FFVVGLEVKRELICGQLSSPEQRTLPVLAAIAGMAVPAIVYVGVVGTDSALVRGWAIPAATDIAFAMGVLGLLGSRVPAS
LRLFLLTVAIVDDIGAVLVIAAFYTANLKVMWLVIALGIFGVMVGMNKFGVDRIWPYILVALVLWVAVLFSGVHATIAGV
MAALTIPMRRKDGHSLLEKLEHGLAPWSAYLVVPIFGFANAGVNLSGMGLDAVLAPLPLAIAAGLVVGKQLGIFGIIVAA
VKLGIAKAPANANWIEIWGVSILTGIGFTMSLFISGLAFTDSRLLIDEAKIGILGGSLISAILGYTILRLTTTHPEERPP
QTVTP
>Mature_405_residues
MVDPIRSPLSRAFAPVRALFVSDASAGILLILVAAAAMIVANSPLAGAYEEMFYGDLAWTPIAKLDDLHLWINDGLMAIF
FFVVGLEVKRELICGQLSSPEQRTLPVLAAIAGMAVPAIVYVGVVGTDSALVRGWAIPAATDIAFAMGVLGLLGSRVPAS
LRLFLLTVAIVDDIGAVLVIAAFYTANLKVMWLVIALGIFGVMVGMNKFGVDRIWPYILVALVLWVAVLFSGVHATIAGV
MAALTIPMRRKDGHSLLEKLEHGLAPWSAYLVVPIFGFANAGVNLSGMGLDAVLAPLPLAIAAGLVVGKQLGIFGIIVAA
VKLGIAKAPANANWIEIWGVSILTGIGFTMSLFISGLAFTDSRLLIDEAKIGILGGSLISAILGYTILRLTTTHPEERPP
QTVTP

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=382, Percent_Identity=50, Blast_Score=305, Evalue=3e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA1_ERYLH (Q2NDA1)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_457137.1
- ProteinModelPortal:   Q2NDA1
- SMR:   Q2NDA1
- STRING:   Q2NDA1
- GeneID:   3871730
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_01240
- NMPDR:   fig|314225.3.peg.2840
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   ANANWIE
- PhylomeDB:   Q2NDA1
- BioCyc:   ELIT314225:ELI_01240-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670
- TIGRFAMs:   TIGR00773

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 42597; Mature: 42597

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x12402564)-; HASH(0x134ddd94)-; HASH(0x12d8d2a8)-; HASH(0x134e7acc)-; HASH(0x12f55398)-; HASH(0x12e92b60)-; HASH(0x12cf0900)-; HASH(0x134e96ec)-; HASH(0x134e95b4)-; HASH(0x134e7c10)-; HASH(0x12c90888)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDPIRSPLSRAFAPVRALFVSDASAGILLILVAAAAMIVANSPLAGAYEEMFYGDLAWT
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
PIAKLDDLHLWINDGLMAIFFFVVGLEVKRELICGQLSSPEQRTLPVLAAIAGMAVPAIV
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
YVGVVGTDSALVRGWAIPAATDIAFAMGVLGLLGSRVPASLRLFLLTVAIVDDIGAVLVI
HEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAFYTANLKVMWLVIALGIFGVMVGMNKFGVDRIWPYILVALVLWVAVLFSGVHATIAGV
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAALTIPMRRKDGHSLLEKLEHGLAPWSAYLVVPIFGFANAGVNLSGMGLDAVLAPLPLA
HHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHH
IAAGLVVGKQLGIFGIIVAAVKLGIAKAPANANWIEIWGVSILTGIGFTMSLFISGLAFT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
DSRLLIDEAKIGILGGSLISAILGYTILRLTTTHPEERPPQTVTP
CCEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
>Mature Secondary Structure
MVDPIRSPLSRAFAPVRALFVSDASAGILLILVAAAAMIVANSPLAGAYEEMFYGDLAWT
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
PIAKLDDLHLWINDGLMAIFFFVVGLEVKRELICGQLSSPEQRTLPVLAAIAGMAVPAIV
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
YVGVVGTDSALVRGWAIPAATDIAFAMGVLGLLGSRVPASLRLFLLTVAIVDDIGAVLVI
HEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAFYTANLKVMWLVIALGIFGVMVGMNKFGVDRIWPYILVALVLWVAVLFSGVHATIAGV
HHHHHCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAALTIPMRRKDGHSLLEKLEHGLAPWSAYLVVPIFGFANAGVNLSGMGLDAVLAPLPLA
HHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHH
IAAGLVVGKQLGIFGIIVAAVKLGIAKAPANANWIEIWGVSILTGIGFTMSLFISGLAFT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHC
DSRLLIDEAKIGILGGSLISAILGYTILRLTTTHPEERPPQTVTP
CCEEEEECCCCEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA