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The map label for this gene is rpoH [H]

Identifier: 85373062

GI number: 85373062

Start: 272331

End: 273236

Strand: Reverse

Name: rpoH [H]

Synonym: ELI_01175

Alternate gene names: 85373062

Gene position: 273236-272331 (Counterclockwise)

Preceding gene: 85373063

Following gene: 85373061

Centisome position: 8.95

GC content: 61.92

Gene sequence:

>906_bases
GTGAGCAAATCAAAAGCAGTCGCCATTCCGGCGCTGGGCGGTGAGCAGAGCCTCAACCGCTATCTCTCCGAAATCAAGAA
ATACCCCGTGCTGACCGCCGAGCAGGAATATATGCTCGCCAAGCGGTATCAGGAGCATGAGGATCCGGAAGCCGCCGCAC
AGCTGGTCTCCAGCCATCTGCGGCTCGTGGCCAAGATCGCGATGGGATATCGCGGCTACGGCCTGCCGGTCAGCGACCTC
ATTTCCGAAGGCAATGTCGGGCTGATGCAGGGCGTGAAAAAGTTCGAGCCCGATCGTGGCTTCCGCCTCGCGACCTATGC
GATGTGGTGGATCAAGGCGAGCATGCAGGAGTTCATCTTGCGCAGCTGGTCGCTCGTAAAAATGGGCACCACCGCCGCGC
AGAAGAAGCTGTTCTTCAACCTGCGCCGGATGAAGAAGCAGCTCGAGGCCTATGAAGACACCGACCTGAGCCCCGAAGAC
GTAACCAAGATCGCAACCGACCTCGGCGTGCCCGAGCAGGAAGTGATCAACATGAACCGCCGGATGATGATGGGCGGCGA
TGGCTCGCTCAACACACCGATGCGCAATGGCGAGGACGGCTCGGGCGAGTGGCAGGACTGGTTGACCGACGATCGCCCGC
TGCAGGACGAGACCGTGGCCAACGCCGAAGAGGCGCAGGTCCGGCACGAAATGCTGGTCGAGGCGATGGACGCGCTCAAC
GAGCGTGAGCAGCACATCCTGACCGAACGCCGACTGACGGAAAACCCGCAGACGCTGGAAGAGCTCTCGCAGGTCTACGA
TGTCAGCCGCGAACGCATCCGCCAGATCGAGGTGCGCGCTTTCGAGAAGCTGCAGAAGGCGATGCAGCGGATCGCTGGCG
AGAGGCTGCTGCCCGGCGCGGCGTAA

Upstream 100 bases:

>100_bases
TCCGTGGGCATTGCTCTATATGTAACCATGTGGCCCACCCGCACGGATGCCCAGCAGGGGTCCGGCATCTGAGGTCGACG
CTAGAAAGGTTAGGGAAGAC

Downstream 100 bases:

>100_bases
GTCGTTTCGCGAATTCTTGACGAGGCCCCGTTCCTGCAAGGTCGGGGCCTCGTTGCATTTGGCGATAAGCGAACCTAATC
CAGCGCCATGCTCTGGTTCG

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSKSKAVAIPALGGEQSLNRYLSEIKKYPVLTAEQEYMLAKRYQEHEDPEAAAQLVSSHLRLVAKIAMGYRGYGLPVSDL
ISEGNVGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKQLEAYEDTDLSPED
VTKIATDLGVPEQEVINMNRRMMMGGDGSLNTPMRNGEDGSGEWQDWLTDDRPLQDETVANAEEAQVRHEMLVEAMDALN
EREQHILTERRLTENPQTLEELSQVYDVSRERIRQIEVRAFEKLQKAMQRIAGERLLPGAA

Sequences:

>Translated_301_residues
MSKSKAVAIPALGGEQSLNRYLSEIKKYPVLTAEQEYMLAKRYQEHEDPEAAAQLVSSHLRLVAKIAMGYRGYGLPVSDL
ISEGNVGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKQLEAYEDTDLSPED
VTKIATDLGVPEQEVINMNRRMMMGGDGSLNTPMRNGEDGSGEWQDWLTDDRPLQDETVANAEEAQVRHEMLVEAMDALN
EREQHILTERRLTENPQTLEELSQVYDVSRERIRQIEVRAFEKLQKAMQRIAGERLLPGAA
>Mature_300_residues
SKSKAVAIPALGGEQSLNRYLSEIKKYPVLTAEQEYMLAKRYQEHEDPEAAAQLVSSHLRLVAKIAMGYRGYGLPVSDLI
SEGNVGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFILRSWSLVKMGTTAAQKKLFFNLRRMKKQLEAYEDTDLSPEDV
TKIATDLGVPEQEVINMNRRMMMGGDGSLNTPMRNGEDGSGEWQDWLTDDRPLQDETVANAEEAQVRHEMLVEAMDALNE
REQHILTERRLTENPQTLEELSQVYDVSRERIRQIEVRAFEKLQKAMQRIAGERLLPGAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=300, Percent_Identity=38.6666666666667, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI1789098, Length=274, Percent_Identity=32.4817518248175, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1789448, Length=271, Percent_Identity=31.3653136531365, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34356; Mature: 34225

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSKAVAIPALGGEQSLNRYLSEIKKYPVLTAEQEYMLAKRYQEHEDPEAAAQLVSSHL
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RLVAKIAMGYRGYGLPVSDLISEGNVGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFIL
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RSWSLVKMGTTAAQKKLFFNLRRMKKQLEAYEDTDLSPEDVTKIATDLGVPEQEVINMNR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCC
RMMMGGDGSLNTPMRNGEDGSGEWQDWLTDDRPLQDETVANAEEAQVRHEMLVEAMDALN
CEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
EREQHILTERRLTENPQTLEELSQVYDVSRERIRQIEVRAFEKLQKAMQRIAGERLLPGA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
A
C
>Mature Secondary Structure 
SKSKAVAIPALGGEQSLNRYLSEIKKYPVLTAEQEYMLAKRYQEHEDPEAAAQLVSSHL
CCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHH
RLVAKIAMGYRGYGLPVSDLISEGNVGLMQGVKKFEPDRGFRLATYAMWWIKASMQEFIL
HHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
RSWSLVKMGTTAAQKKLFFNLRRMKKQLEAYEDTDLSPEDVTKIATDLGVPEQEVINMNR
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHCCCC
RMMMGGDGSLNTPMRNGEDGSGEWQDWLTDDRPLQDETVANAEEAQVRHEMLVEAMDALN
CEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
EREQHILTERRLTENPQTLEELSQVYDVSRERIRQIEVRAFEKLQKAMQRIAGERLLPGA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]