Click here to switch to the map view.

The map label for this gene is sppA [H]

Identifier: 85373032

GI number: 85373032

Start: 241588

End: 243468

Strand: Reverse

Name: sppA [H]

Synonym: ELI_01025

Alternate gene names: 85373032

Gene position: 243468-241588 (Counterclockwise)

Preceding gene: 85373036

Following gene: 85373031

Centisome position: 7.98

GC content: 65.71

Gene sequence:

>1881_bases
ATGGCGTTCGCAAAAGGTGTCTGGAAACTGCTGGTCGGGATCAAGGACGGTCTCAGCCTGCTGTTTCTGCTGCTCTTCTT
CATGGCGCTCTATGCCGTCCTCACCGCGCGCCCGAGCCCGGCACAGGTCCGCGAAGGCGCGCTGTTGCTCGAGCTCGACG
GCGTCGTGGTCGAGGAAAAGAGCGTGATCGACCCGCTCGAACTCTTTCTCAGCGGAACGGCTCCGATCGGCGAATACGAC
GCGCAGGACCTTGTCCATGCTATCGACGCCGCAACGGGCGACGACCGGATCAAGGCCATCGTGCTCGACCTGACGGGCTT
TCTCGGCGGCGGACATGTGCATTTGCAGGACATCGGCGAAGCCCTGCAACGTGCGCGCGACGCGGAAAAGCCGGTTCTCA
CTTACGGTATCGGCTACGGCGACGACGCGATGATGCTGGCGGCGCATGCCAGCGAAGTCTGGCTGGACCCGCTCGGCGGT
GCAATCATCGCCGGACCCGGTGGCAACCGCCTCTATTTCGGCCAGTTTCTCGAAGACCTGAATATCAACGCCCGCATCTA
CAAGGTGGGCACCTACAAGTCCGCCGTCGAACCCTTCGACCGCAGCGACATGTCACCCGAAGCCCGCGAAAACGCGCAGG
CCCTGTACGGCGCGCTGTGGGAAGAATGGCAGGCCAAGGTCAAGAAAGCGCGCCCTGCCATTGATCTCGACACCGTGACC
GGGTCCCCTGCCGAATGGGTCGCAGCGAGCCAGGGCGATCTGGCGCAGGCGGCGCTTGCGGCAGGCCTCGTCGATACGCT
GGGCAGCCGCGTCGAATTCGGCGAGCGGGTGGCGGAAATCGCCGGCGAGGACGATTGGGAAGAACGCCCGGGCGCCTTCG
CTCACACCGCTTATGACGCGTGGCTTTCCGACAATGCGCGCGACACATCCGGCAGCGCCATCGGCGTTGTCACCGTCGCC
GGAGAAATCGTCGATGGCGAGGCTGGCCCGGGCACCGCTGGCGGCGACCGCATCGCCGAATTGCTCGACGCTGCCCTGTC
CGACGATCTCGCCGGGCTGATCGTCCGCGTCGATTCGCCGGGCGGCTCGGTCCTTGCGAGCGAAGCGATCCGCGATGCGG
TACTGCGGCACAAGGCGAAGGGCATCCCCATCGCCGTTTCGATGGCCAATGTCGCCGCCAGTGGCGGCTATTGGGTCTCG
ACCCCGGCCGACCGCATCTTTGCCGAACCGGAGACCATCACTGGATCGATCGGCATTTTCGCCGTCATCCCGACGTTCGA
AAACCTGGCCGCGGAATACGGCGTCAACGCCGACGGCGTGGCGACCACGTCCCTTTCCGGACAGCCGGATCTGATCGCGG
GCTTCACGCCGGAAGTCGATGCGATCCTGCAGAGCTCGATCGAAGACGGCTATCGCGACTTCCTCACCCGCGTGTCGGAA
GCCCGCAAACTCCCAATGGAGCGTGTGGACGCCATCGGCCAGGGCCGCGTATGGGATGGCGGCACTGCGCGCCAGATCGG
CCTGGTCGACCAGTATGGCGGGCTCGAAGAAGCGCTCGATTGGGTCGCCGCCCAGGCCGAACTGGAAGAGGGCGACTGGC
ACGTCCGCTTCCTCGGCAACGAGCAGCAGCCGTTCGACAACCTGCTTCGCACCATGCTGCTCACCGACAGCGAAAGCGCG
CCAGCCGGCGACATATTCGGCATGGTTGCGACTCGCCAGCAGGCGTTGCTGGGGCAGGTCCGTACCGATCTCGAGCGGCT
GCTCGGCACCAGGGGGATGCAGGCCTATTGCCTTGCCTGCCCCGTCACGCCTCGCGCCCGGGCCGAGGCCGCCGCCGACG
ATGGGTGGTTCGCGCAAATGGCGGCATTTTTCGCCCGCTAG

Upstream 100 bases:

>100_bases
AGCCTTTAGTCGAGCCATGTTGAGCGCTCAAGCGGTTTCGGCTGGCGATGCGCGGTTTGGCTGCCTATGTGTCAGCCAGG
CAATACAAGGGTTTTGACGC

Downstream 100 bases:

>100_bases
GTGCTTGTCAGGGCCCGCGCCATTCCGTAAAGGCGCGCCCCTGCCCGGCAGTCAGCGAGTAACAGTCTGCTGACGTGCCG
CACATGGGGCGGGCGCGTAG

Product: periplasmic serine protease

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV; Signal peptide peptidase [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MAFAKGVWKLLVGIKDGLSLLFLLLFFMALYAVLTARPSPAQVREGALLLELDGVVVEEKSVIDPLELFLSGTAPIGEYD
AQDLVHAIDAATGDDRIKAIVLDLTGFLGGGHVHLQDIGEALQRARDAEKPVLTYGIGYGDDAMMLAAHASEVWLDPLGG
AIIAGPGGNRLYFGQFLEDLNINARIYKVGTYKSAVEPFDRSDMSPEARENAQALYGALWEEWQAKVKKARPAIDLDTVT
GSPAEWVAASQGDLAQAALAAGLVDTLGSRVEFGERVAEIAGEDDWEERPGAFAHTAYDAWLSDNARDTSGSAIGVVTVA
GEIVDGEAGPGTAGGDRIAELLDAALSDDLAGLIVRVDSPGGSVLASEAIRDAVLRHKAKGIPIAVSMANVAASGGYWVS
TPADRIFAEPETITGSIGIFAVIPTFENLAAEYGVNADGVATTSLSGQPDLIAGFTPEVDAILQSSIEDGYRDFLTRVSE
ARKLPMERVDAIGQGRVWDGGTARQIGLVDQYGGLEEALDWVAAQAELEEGDWHVRFLGNEQQPFDNLLRTMLLTDSESA
PAGDIFGMVATRQQALLGQVRTDLERLLGTRGMQAYCLACPVTPRARAEAAADDGWFAQMAAFFAR

Sequences:

>Translated_626_residues
MAFAKGVWKLLVGIKDGLSLLFLLLFFMALYAVLTARPSPAQVREGALLLELDGVVVEEKSVIDPLELFLSGTAPIGEYD
AQDLVHAIDAATGDDRIKAIVLDLTGFLGGGHVHLQDIGEALQRARDAEKPVLTYGIGYGDDAMMLAAHASEVWLDPLGG
AIIAGPGGNRLYFGQFLEDLNINARIYKVGTYKSAVEPFDRSDMSPEARENAQALYGALWEEWQAKVKKARPAIDLDTVT
GSPAEWVAASQGDLAQAALAAGLVDTLGSRVEFGERVAEIAGEDDWEERPGAFAHTAYDAWLSDNARDTSGSAIGVVTVA
GEIVDGEAGPGTAGGDRIAELLDAALSDDLAGLIVRVDSPGGSVLASEAIRDAVLRHKAKGIPIAVSMANVAASGGYWVS
TPADRIFAEPETITGSIGIFAVIPTFENLAAEYGVNADGVATTSLSGQPDLIAGFTPEVDAILQSSIEDGYRDFLTRVSE
ARKLPMERVDAIGQGRVWDGGTARQIGLVDQYGGLEEALDWVAAQAELEEGDWHVRFLGNEQQPFDNLLRTMLLTDSESA
PAGDIFGMVATRQQALLGQVRTDLERLLGTRGMQAYCLACPVTPRARAEAAADDGWFAQMAAFFAR
>Mature_625_residues
AFAKGVWKLLVGIKDGLSLLFLLLFFMALYAVLTARPSPAQVREGALLLELDGVVVEEKSVIDPLELFLSGTAPIGEYDA
QDLVHAIDAATGDDRIKAIVLDLTGFLGGGHVHLQDIGEALQRARDAEKPVLTYGIGYGDDAMMLAAHASEVWLDPLGGA
IIAGPGGNRLYFGQFLEDLNINARIYKVGTYKSAVEPFDRSDMSPEARENAQALYGALWEEWQAKVKKARPAIDLDTVTG
SPAEWVAASQGDLAQAALAAGLVDTLGSRVEFGERVAEIAGEDDWEERPGAFAHTAYDAWLSDNARDTSGSAIGVVTVAG
EIVDGEAGPGTAGGDRIAELLDAALSDDLAGLIVRVDSPGGSVLASEAIRDAVLRHKAKGIPIAVSMANVAASGGYWVST
PADRIFAEPETITGSIGIFAVIPTFENLAAEYGVNADGVATTSLSGQPDLIAGFTPEVDAILQSSIEDGYRDFLTRVSEA
RKLPMERVDAIGQGRVWDGGTARQIGLVDQYGGLEEALDWVAAQAELEEGDWHVRFLGNEQQPFDNLLRTMLLTDSESAP
AGDIFGMVATRQQALLGQVRTDLERLLGTRGMQAYCLACPVTPRARAEAAADDGWFAQMAAFFAR

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=621, Percent_Identity=35.9098228663446, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1787527, Length=170, Percent_Identity=27.0588235294118, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.- [C]

Molecular weight: Translated: 66524; Mature: 66392

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFAKGVWKLLVGIKDGLSLLFLLLFFMALYAVLTARPSPAQVREGALLLELDGVVVEEK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCEEECCH
SVIDPLELFLSGTAPIGEYDAQDLVHAIDAATGDDRIKAIVLDLTGFLGGGHVHLQDIGE
HHCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEEHHHHCCCCEEEHHHHHH
ALQRARDAEKPVLTYGIGYGDDAMMLAAHASEVWLDPLGGAIIAGPGGNRLYFGQFLEDL
HHHHHHCCCCCEEEEECCCCCCHHEEEHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHCC
NINARIYKVGTYKSAVEPFDRSDMSPEARENAQALYGALWEEWQAKVKKARPAIDLDTVT
CCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
GSPAEWVAASQGDLAQAALAAGLVDTLGSRVEFGERVAEIAGEDDWEERPGAFAHTAYDA
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
WLSDNARDTSGSAIGVVTVAGEIVDGEAGPGTAGGDRIAELLDAALSDDLAGLIVRVDSP
HHCCCCCCCCCCEEEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECC
GGSVLASEAIRDAVLRHKAKGIPIAVSMANVAASGGYWVSTPADRIFAEPETITGSIGIF
CCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCEEEECCHHHHCCCCCCEECCCEEE
AVIPTFENLAAEYGVNADGVATTSLSGQPDLIAGFTPEVDAILQSSIEDGYRDFLTRVSE
EEECCHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ARKLPMERVDAIGQGRVWDGGTARQIGLVDQYGGLEEALDWVAAQAELEEGDWHVRFLGN
HHCCCHHHHHCCCCCCCCCCCCCCEECCHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECC
EQQPFDNLLRTMLLTDSESAPAGDIFGMVATRQQALLGQVRTDLERLLGTRGMQAYCLAC
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PVTPRARAEAAADDGWFAQMAAFFAR
CCCCCHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AFAKGVWKLLVGIKDGLSLLFLLLFFMALYAVLTARPSPAQVREGALLLELDGVVVEEK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCEEEEEECCEEECCH
SVIDPLELFLSGTAPIGEYDAQDLVHAIDAATGDDRIKAIVLDLTGFLGGGHVHLQDIGE
HHCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEEHHHHCCCCEEEHHHHHH
ALQRARDAEKPVLTYGIGYGDDAMMLAAHASEVWLDPLGGAIIAGPGGNRLYFGQFLEDL
HHHHHHCCCCCEEEEECCCCCCHHEEEHHHHHHHCCCCCCEEEECCCCCEEEHHHHHHCC
NINARIYKVGTYKSAVEPFDRSDMSPEARENAQALYGALWEEWQAKVKKARPAIDLDTVT
CCCEEEEEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
GSPAEWVAASQGDLAQAALAAGLVDTLGSRVEFGERVAEIAGEDDWEERPGAFAHTAYDA
CCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHH
WLSDNARDTSGSAIGVVTVAGEIVDGEAGPGTAGGDRIAELLDAALSDDLAGLIVRVDSP
HHCCCCCCCCCCEEEEEEEECEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEECC
GGSVLASEAIRDAVLRHKAKGIPIAVSMANVAASGGYWVSTPADRIFAEPETITGSIGIF
CCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCCCCEEEECCHHHHCCCCCCEECCCEEE
AVIPTFENLAAEYGVNADGVATTSLSGQPDLIAGFTPEVDAILQSSIEDGYRDFLTRVSE
EEECCHHHHHHHHCCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHH
ARKLPMERVDAIGQGRVWDGGTARQIGLVDQYGGLEEALDWVAAQAELEEGDWHVRFLGN
HHCCCHHHHHCCCCCCCCCCCCCCEECCHHHCCCHHHHHHHHHHHHHCCCCCEEEEEECC
EQQPFDNLLRTMLLTDSESAPAGDIFGMVATRQQALLGQVRTDLERLLGTRGMQAYCLAC
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
PVTPRARAEAAADDGWFAQMAAFFAR
CCCCCHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]