Click here to switch to the map view.

The map label for this gene is benM [H]

Identifier: 85373028

GI number: 85373028

Start: 238339

End: 239163

Strand: Reverse

Name: benM [H]

Synonym: ELI_01005

Alternate gene names: 85373028

Gene position: 239163-238339 (Counterclockwise)

Preceding gene: 85373029

Following gene: 85373020

Centisome position: 7.84

GC content: 56.61

Gene sequence:

>825_bases
GTGACTAAGAGCGTCGCCGATCTGGAGCAACAACTCGGAATTGCTCTCTTCAATCGAACGTCGCGCGGCATCTTGGTGAC
GCCTGAGGGTCGCGACTTCATCGATCGCGCGGCGCGCTTGCTCGCGGATGCAGACGATTTGTTCAGAGAACGCAATCGTG
GTGCTGATCCCTACGCTGGGCTCCTCAGAATTGGCCTTTTTCCAGGTTCAATGGACTGGCTAATCAACGACGCCGCAATC
GGGTTGCTCAAACGCCACGAGGGAGTTCGTCTCGAGATCGTATCGGGAAATAGTGAGCGCGCAGTGCAATTGCTGTCGCG
CGGAGACATCGACGTCGCCTTCGGGCTCGAGGCTGCGCTTGCCGGTTGGCCTCAGTTCAAATGCGAGCGGATTGCAGCGG
TTGATATCATGCCTTTCGTTCGCAAAGACCATCCGATCCTTTCCGAAACTGCAATCGATAAGCAGATGTTGGCGCGATGC
GACTTCGTCGTCCCTTCGTCGTCCGAGCCGTATACACCTATCATTCGACAGATATACGAAGTGAACGATCAGCGCCCGGA
AGATCGCATCCATATGACGGATTATTTTCCGCTCGTTCGCAGGATCGTGGCCGCTACCGATTCCGTCGGATTGGTGGCTA
GCGCATTCACCTCTGCCCGATGGTTTCGCGACGAATTTGTCACGCTGCCACATACTGGTCTGCTGGAACCGCTGGTGCTA
GCGTACGCCGCACGTGCTCGCTGGCCGGTCAAGCCCGCCGCTAGGGATCTCATCGCCCGAGTGCGACAGAATTGGTCATC
AGAGTCTAATGCTCAGAAACAGTAG

Upstream 100 bases:

>100_bases
CTCATCTGTGCTTGATTCCCGTCTCAAATATGCGGTCGCAGTTGCTCGAATAGGTTCCTTCACAGGAGCGGCCAATGCCG
TGGGCGTGACACAGTCAGCT

Downstream 100 bases:

>100_bases
GCTGGAGCAAGTATATGTCCGGCCATATGCATGCTCGCATCGTAAAGGTCAAATGACAGACCCTCAGTATATAGCCTGTC
CCGATTTTGTGGAATCGGAA

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Ben and cat operon transcriptional regulator [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTKSVADLEQQLGIALFNRTSRGILVTPEGRDFIDRAARLLADADDLFRERNRGADPYAGLLRIGLFPGSMDWLINDAAI
GLLKRHEGVRLEIVSGNSERAVQLLSRGDIDVAFGLEAALAGWPQFKCERIAAVDIMPFVRKDHPILSETAIDKQMLARC
DFVVPSSSEPYTPIIRQIYEVNDQRPEDRIHMTDYFPLVRRIVAATDSVGLVASAFTSARWFRDEFVTLPHTGLLEPLVL
AYAARARWPVKPAARDLIARVRQNWSSESNAQKQ

Sequences:

>Translated_274_residues
MTKSVADLEQQLGIALFNRTSRGILVTPEGRDFIDRAARLLADADDLFRERNRGADPYAGLLRIGLFPGSMDWLINDAAI
GLLKRHEGVRLEIVSGNSERAVQLLSRGDIDVAFGLEAALAGWPQFKCERIAAVDIMPFVRKDHPILSETAIDKQMLARC
DFVVPSSSEPYTPIIRQIYEVNDQRPEDRIHMTDYFPLVRRIVAATDSVGLVASAFTSARWFRDEFVTLPHTGLLEPLVL
AYAARARWPVKPAARDLIARVRQNWSSESNAQKQ
>Mature_273_residues
TKSVADLEQQLGIALFNRTSRGILVTPEGRDFIDRAARLLADADDLFRERNRGADPYAGLLRIGLFPGSMDWLINDAAIG
LLKRHEGVRLEIVSGNSERAVQLLSRGDIDVAFGLEAALAGWPQFKCERIAAVDIMPFVRKDHPILSETAIDKQMLARCD
FVVPSSSEPYTPIIRQIYEVNDQRPEDRIHMTDYFPLVRRIVAATDSVGLVASAFTSARWFRDEFVTLPHTGLLEPLVLA
YAARARWPVKPAARDLIARVRQNWSSESNAQKQ

Specific function: Positive regulator of the ben and cat genes for benzoate degradation. BenM is necessary for ben gene expression but not for expression of the cat genes, which can be regulated by CatM. Binds to the inducers cis,cis-muconate and benzoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30727; Mature: 30595

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSVADLEQQLGIALFNRTSRGILVTPEGRDFIDRAARLLADADDLFRERNRGADPYAG
CCCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHH
LLRIGLFPGSMDWLINDAAIGLLKRHEGVRLEIVSGNSERAVQLLSRGDIDVAFGLEAAL
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEECHHHHH
AGWPQFKCERIAAVDIMPFVRKDHPILSETAIDKQMLARCDFVVPSSSEPYTPIIRQIYE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHH
VNDQRPEDRIHMTDYFPLVRRIVAATDSVGLVASAFTSARWFRDEFVTLPHTGLLEPLVL
HCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEECCCCCHHHHHHH
AYAARARWPVKPAARDLIARVRQNWSSESNAQKQ
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TKSVADLEQQLGIALFNRTSRGILVTPEGRDFIDRAARLLADADDLFRERNRGADPYAG
CCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHH
LLRIGLFPGSMDWLINDAAIGLLKRHEGVRLEIVSGNSERAVQLLSRGDIDVAFGLEAAL
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHCCCCEEEECHHHHH
AGWPQFKCERIAAVDIMPFVRKDHPILSETAIDKQMLARCDFVVPSSSEPYTPIIRQIYE
CCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHH
VNDQRPEDRIHMTDYFPLVRRIVAATDSVGLVASAFTSARWFRDEFVTLPHTGLLEPLVL
HCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEECCCCCHHHHHHH
AYAARARWPVKPAARDLIARVRQNWSSESNAQKQ
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9573203 [H]