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The map label for this gene is glpK [H]

Identifier: 85372923

GI number: 85372923

Start: 126049

End: 127521

Strand: Direct

Name: glpK [H]

Synonym: ELI_00480

Alternate gene names: 85372923

Gene position: 126049-127521 (Clockwise)

Preceding gene: 85372916

Following gene: 85372926

Centisome position: 4.13

GC content: 67.68

Gene sequence:

>1473_bases
ATGCGCTTGTCCGACCTGATCCTCGTCTTCGATGAAGGCACCACATCTACCCGCGCCATGCTGTTCGGGCTCGACGGCTC
GCTGCACGGCGTTTCGCAGAAGGAACTAACCCAGCATTATCCCAGGCCCGGCTGGGTCGAGCACGATGCTGGCGAGATAT
GGGAGCGGACGCTGGCGTGCGCGCGGGCAGTAGTGGAAAGGGTCGGCGCGGATCGGATCGCCTGCATCGGCATCACCAAC
CAGCGCGAAACGGTGGTGGCGTGGGACGCCCAGACGCTTGAGCCGCTGACCCGCGCGATCGTGTGGCAGGATCGGCGGAC
GGCCGATTTCTGCGAAGAGTTGCGCGCGGCGGGGCACGAGGCCGAGATCCAGAAAAGGACCGGCCTACTGCTCGACCCCT
ATTTCTCGGGCACCAAAATGCGCTGGCTGCTCGACCACGACGAGCGCGTGAAGCAGGCGGCGCGCAAGGGCACACTGGCG
CTCGGCACAATCGAAAGCTGGCTGGTCGCGAAACTGAGCGGCGGGGCGCATGTCTCGGACGCCAGCAATGCCAGCCGCAC
GCAACTGCTCGCGCTCGACGGGGCGCAATTCGACGAAGGATTGTGCGACCTGCTCGGCGTGCCGCGAAGGGCGCTGCCCG
AAGTGGTCGATACGCAGGGGCGCTTGGCTGAGGCAAAGCCAGACTGGCTGGGCCGCGCGATCCCGATCACCGGCATGGTC
GGCGACCAGCAATCGGCGACGCTGGGGCAAGGCTGCCTGTCGGTCGGCAATACCAAGGCGACCTACGGCACCGGTGCCTT
CGTACTCGCCAACAAGGGCAGCGAGTTGCCGCGCTCGGACAACCGCCTGCTCGGCACGGTGCTGTATCAGCAAGGCGGAG
AGCGGACCTACGCGATCGAAGGATCGGTCTTCGTCGCCGGCAGTCTCGTCCAGTGGCTGCGCGACCAGCTGGGCCTGATC
TCCAGCGCGGCGGAAACCGAAGCGCTGGCGCGCGCCATTCCCGACAGCGGCGAAGTCGTGATCGTGCCCGCGCTCTCGGG
CCTTGGCGCGCCGCATTGGCGGGCCGAGGCGCGCGGGGTGATCGCCGGGTTGAGTTTTTCCAGCGGCAAGGCGCAGATCG
CCCGCGCAGCGCTGGAAGCGATGGCGCACCAGACCTACGATCTGGCGCAGGCCTTCGCCGCCGACGGCGCGGAATGGACC
GGACTCAAGATCGACGGCGGGATGAGCGCCAACGACTGGATGGCGCAGGACCTCGCCGACATGCTCGACCTGACCGTGGA
GCGCCCGGACTTTGTGGAGACCACTGCACTGGGCGCGGCGATGAGCGCGGCGGTCGGAGCAGGGCTGCACCCTTCTCTCG
AGGACGCAGCCAAGCACATGCGCGGCAGGGTCACGAGCTTCGATCCGGCGATGGACGCCGCAATACGGGAAGAGCGTCTC
GCGCGCTGGCGCAAGGCGCTGGCTGCGTCCTAG

Upstream 100 bases:

>100_bases
TTGCCGGTCGGCCACGGCTTGGGAGGGATATCGGCCATGCCCTTGCATATGGGATGGAAGCTGGCCCATGTCACGCCACA
AGCAATTTGCACGTGGAGAG

Downstream 100 bases:

>100_bases
GCGCTTTCCGACATTTGCCGGATCGCGCGAGTCAGGCGTTCCTGCAACTCGTCGATCGGGCTTGCGGGAAATTCGAACCG
CTCGCGCCAGGCCCGGTCGA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MRLSDLILVFDEGTTSTRAMLFGLDGSLHGVSQKELTQHYPRPGWVEHDAGEIWERTLACARAVVERVGADRIACIGITN
QRETVVAWDAQTLEPLTRAIVWQDRRTADFCEELRAAGHEAEIQKRTGLLLDPYFSGTKMRWLLDHDERVKQAARKGTLA
LGTIESWLVAKLSGGAHVSDASNASRTQLLALDGAQFDEGLCDLLGVPRRALPEVVDTQGRLAEAKPDWLGRAIPITGMV
GDQQSATLGQGCLSVGNTKATYGTGAFVLANKGSELPRSDNRLLGTVLYQQGGERTYAIEGSVFVAGSLVQWLRDQLGLI
SSAAETEALARAIPDSGEVVIVPALSGLGAPHWRAEARGVIAGLSFSSGKAQIARAALEAMAHQTYDLAQAFAADGAEWT
GLKIDGGMSANDWMAQDLADMLDLTVERPDFVETTALGAAMSAAVGAGLHPSLEDAAKHMRGRVTSFDPAMDAAIREERL
ARWRKALAAS

Sequences:

>Translated_490_residues
MRLSDLILVFDEGTTSTRAMLFGLDGSLHGVSQKELTQHYPRPGWVEHDAGEIWERTLACARAVVERVGADRIACIGITN
QRETVVAWDAQTLEPLTRAIVWQDRRTADFCEELRAAGHEAEIQKRTGLLLDPYFSGTKMRWLLDHDERVKQAARKGTLA
LGTIESWLVAKLSGGAHVSDASNASRTQLLALDGAQFDEGLCDLLGVPRRALPEVVDTQGRLAEAKPDWLGRAIPITGMV
GDQQSATLGQGCLSVGNTKATYGTGAFVLANKGSELPRSDNRLLGTVLYQQGGERTYAIEGSVFVAGSLVQWLRDQLGLI
SSAAETEALARAIPDSGEVVIVPALSGLGAPHWRAEARGVIAGLSFSSGKAQIARAALEAMAHQTYDLAQAFAADGAEWT
GLKIDGGMSANDWMAQDLADMLDLTVERPDFVETTALGAAMSAAVGAGLHPSLEDAAKHMRGRVTSFDPAMDAAIREERL
ARWRKALAAS
>Mature_490_residues
MRLSDLILVFDEGTTSTRAMLFGLDGSLHGVSQKELTQHYPRPGWVEHDAGEIWERTLACARAVVERVGADRIACIGITN
QRETVVAWDAQTLEPLTRAIVWQDRRTADFCEELRAAGHEAEIQKRTGLLLDPYFSGTKMRWLLDHDERVKQAARKGTLA
LGTIESWLVAKLSGGAHVSDASNASRTQLLALDGAQFDEGLCDLLGVPRRALPEVVDTQGRLAEAKPDWLGRAIPITGMV
GDQQSATLGQGCLSVGNTKATYGTGAFVLANKGSELPRSDNRLLGTVLYQQGGERTYAIEGSVFVAGSLVQWLRDQLGLI
SSAAETEALARAIPDSGEVVIVPALSGLGAPHWRAEARGVIAGLSFSSGKAQIARAALEAMAHQTYDLAQAFAADGAEWT
GLKIDGGMSANDWMAQDLADMLDLTVERPDFVETTALGAAMSAAVGAGLHPSLEDAAKHMRGRVTSFDPAMDAAIREERL
ARWRKALAAS

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI4504007, Length=508, Percent_Identity=40.3543307086614, Blast_Score=345, Evalue=5e-95,
Organism=Homo sapiens, GI189409120, Length=508, Percent_Identity=40.3543307086614, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=39.9610136452242, Blast_Score=343, Evalue=3e-94,
Organism=Homo sapiens, GI41393575, Length=495, Percent_Identity=40.2020202020202, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=27.7013752455796, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=43.0020283975659, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI1790005, Length=503, Percent_Identity=25.6461232604374, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1789987, Length=439, Percent_Identity=23.6902050113895, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1787789, Length=488, Percent_Identity=27.4590163934426, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535599, Length=505, Percent_Identity=37.029702970297, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI71986140, Length=479, Percent_Identity=25.2609603340292, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71986147, Length=256, Percent_Identity=28.515625, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321755, Length=492, Percent_Identity=32.9268292682927, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI17864214, Length=500, Percent_Identity=38.4, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24654573, Length=444, Percent_Identity=39.6396396396396, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24655555, Length=501, Percent_Identity=34.9301397205589, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655560, Length=501, Percent_Identity=34.9301397205589, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655570, Length=501, Percent_Identity=34.9301397205589, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655574, Length=501, Percent_Identity=34.9301397205589, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655565, Length=501, Percent_Identity=34.9301397205589, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=30.4511278195489, Blast_Score=232, Evalue=5e-61,
Organism=Drosophila melanogaster, GI24652871, Length=487, Percent_Identity=31.211498973306, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI21357867, Length=523, Percent_Identity=25.4302103250478, Blast_Score=177, Evalue=1e-44,
Organism=Drosophila melanogaster, GI24656367, Length=512, Percent_Identity=25.9765625, Blast_Score=176, Evalue=5e-44,
Organism=Drosophila melanogaster, GI24656372, Length=365, Percent_Identity=28.2191780821918, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI45552909, Length=266, Percent_Identity=28.1954887218045, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 52550; Mature: 52550

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSDLILVFDEGTTSTRAMLFGLDGSLHGVSQKELTQHYPRPGWVEHDAGEIWERTLAC
CCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ARAVVERVGADRIACIGITNQRETVVAWDAQTLEPLTRAIVWQDRRTADFCEELRAAGHE
HHHHHHHCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCH
AEIQKRTGLLLDPYFSGTKMRWLLDHDERVKQAARKGTLALGTIESWLVAKLSGGAHVSD
HHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCC
ASNASRTQLLALDGAQFDEGLCDLLGVPRRALPEVVDTQGRLAEAKPDWLGRAIPITGMV
CCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEC
GDQQSATLGQGCLSVGNTKATYGTGAFVLANKGSELPRSDNRLLGTVLYQQGGERTYAIE
CCCCHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEC
GSVFVAGSLVQWLRDQLGLISSAAETEALARAIPDSGEVVIVPALSGLGAPHWRAEARGV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCEE
IAGLSFSSGKAQIARAALEAMAHQTYDLAQAFAADGAEWTGLKIDGGMSANDWMAQDLAD
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHH
MLDLTVERPDFVETTALGAAMSAAVGAGLHPSLEDAAKHMRGRVTSFDPAMDAAIREERL
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ARWRKALAAS
HHHHHHHCCC
>Mature Secondary Structure
MRLSDLILVFDEGTTSTRAMLFGLDGSLHGVSQKELTQHYPRPGWVEHDAGEIWERTLAC
CCCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHH
ARAVVERVGADRIACIGITNQRETVVAWDAQTLEPLTRAIVWQDRRTADFCEELRAAGHE
HHHHHHHCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCH
AEIQKRTGLLLDPYFSGTKMRWLLDHDERVKQAARKGTLALGTIESWLVAKLSGGAHVSD
HHHHHHCCCEECCCCCCCEEEEEECCHHHHHHHHHCCCEEEEHHHHHHHHHCCCCCCCCC
ASNASRTQLLALDGAQFDEGLCDLLGVPRRALPEVVDTQGRLAEAKPDWLGRAIPITGMV
CCCCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEC
GDQQSATLGQGCLSVGNTKATYGTGAFVLANKGSELPRSDNRLLGTVLYQQGGERTYAIE
CCCCHHHHHHHHHHCCCCCEECCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCEEEEEC
GSVFVAGSLVQWLRDQLGLISSAAETEALARAIPDSGEVVIVPALSGLGAPHWRAEARGV
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHCCEE
IAGLSFSSGKAQIARAALEAMAHQTYDLAQAFAADGAEWTGLKIDGGMSANDWMAQDLAD
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHH
MLDLTVERPDFVETTALGAAMSAAVGAGLHPSLEDAAKHMRGRVTSFDPAMDAAIREERL
HHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ARWRKALAAS
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA