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The map label for this gene is merA [H]

Identifier: 85372897

GI number: 85372897

Start: 95835

End: 97253

Strand: Reverse

Name: merA [H]

Synonym: ELI_00350

Alternate gene names: 85372897

Gene position: 97253-95835 (Counterclockwise)

Preceding gene: 85372900

Following gene: 85372896

Centisome position: 3.19

GC content: 61.17

Gene sequence:

>1419_bases
ATGAAATTCACGCACGACGTTATTGTTATCGGCGGTGGTGCCGCAGGACTGACCGCAGCGGGCGGCTGTGCGCTGTTCGG
GCTGAAAGTGGCGCTGATCGAGGGTCACAAGATGGGCGGCGAGTGCCTCAACAACGGCTGCGTCCCTTCCAAGGCACTGA
TAACCGCCGCAAAGCGCGCTGCAGAAGCCAGGAAGCAGAAACGCTTCGGTGTAGAGCTTGCCGCCCCGAATGTAGAGTGG
AGCGGTGTTCATACACACATTCACCGAGCCATTGCGGAAATCGAGCCGCATGACAGCGCGGAACGGTTCGAAGAGATGGG
CTGCGAAGTAATCCAGGACTGGGCGCGGGTCACCGGCAAGCAGTCGGTCGAAATCGGCGGGCGCACGCTCACCGCACCGC
GGATCGTCATCGCGACCGGCTCAGGCCCCTCGGTGCCGCCGATCCCGGGCCTGGACGCGGTGCCCTATCTCACCAATGAA
AACATCTTCGATCTCGAAGCGCAGCCGGATCATCTGGTGATCATCGGCGGCGGCGTGATCGGGATGGAAATGGCGCAGAG
TTTCGCCCGGCTTGGCAGCAAGGTGACCGTCATCGAGCCGGGCCGTCCGATGGGCCGCGATGATGAGGACTCGGTCGCCG
TCGTGATGGAAGTTATGAAAAAAGAAGGTGTGACCTTCGTGCAGGGCAAGGCCGAGAAGGTCGAAGGCAGGAATGGCGCG
ATTACGGTGCATGTCGACAATGGCGAACAGGTGAGCGGATCGCATCTGCTCATCGCGGTCGGGCGCAAGGCGCGGGTTTC
CGGCTTCGGACTCGAAGATCTGGGCGTCGAACTCGGCAACAACGGGATCAAGGTCGACGAGCGCCGCCGCACTTCGGTGA
AAAACATCTACGCCATCGGCGATTGCCGCGAAGGGCCGCGACTGACGCATGTTAGCGGCTACGAAGGCTCCAATGTGGCG
CTGGAGATTACGCTCGGGATCCCAACCAAGGTCGACTACAAGGCGCTGCCGTGGTGCACCTATACCGAGCCCGAGGTCGC
GCAGATCGGGCTGACCGAAGCGGAAGCGAAAGAAAAGTTCGGCGACAAGGTGACAGTGGTGAAGGAAGGTTTCGACCACA
ATGAGCGCGCCATCACCGAGGGCGACACGAAGGGGCACATGAAGGTCATCCTGAAAGGCAAGAAAGTGCTCGGAGCGAGC
ATCGTCGGCAAGAATGCCGGGGAACTGCTCCTGCCCTTCAGCCAGACGATTACCGGTAAGAGCAGCACGTTCGCCATGGG
CAGCGCAATCGTCAGCTATCCGACCCGTAGTGAGATTACGAAAGCCGCCGCTTTTGCCGCGTGGGAGCCGACCGTCTTTG
GGGCGATCCCGAAGAAATATGCCGGCTTCGTCGCCAAAGTCCGGAGAGCCTTCGCCTGA

Upstream 100 bases:

>100_bases
ATGTAACTTTGCGGCCCCCGGATACGGATTTGAGCGTGACTGCGCCGTGCGACGCAATTGTGATTCGCATATTTCGCAGG
ATGGGTTACGCGAGGGCGGC

Downstream 100 bases:

>100_bases
TGGCCTCCCGCAGCAAGAGCCGCAATGCGCCTGCAGGCGCTTCGCCCTTCGCGGTGGAGGCCGCGCGCCTGCCCGAGGAG
AAGTTCTCCGATCCGGACTG

Product: mercuric reductase, putative

Products: NA

Alternate protein names: Hg(II) reductase [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MKFTHDVIVIGGGAAGLTAAGGCALFGLKVALIEGHKMGGECLNNGCVPSKALITAAKRAAEARKQKRFGVELAAPNVEW
SGVHTHIHRAIAEIEPHDSAERFEEMGCEVIQDWARVTGKQSVEIGGRTLTAPRIVIATGSGPSVPPIPGLDAVPYLTNE
NIFDLEAQPDHLVIIGGGVIGMEMAQSFARLGSKVTVIEPGRPMGRDDEDSVAVVMEVMKKEGVTFVQGKAEKVEGRNGA
ITVHVDNGEQVSGSHLLIAVGRKARVSGFGLEDLGVELGNNGIKVDERRRTSVKNIYAIGDCREGPRLTHVSGYEGSNVA
LEITLGIPTKVDYKALPWCTYTEPEVAQIGLTEAEAKEKFGDKVTVVKEGFDHNERAITEGDTKGHMKVILKGKKVLGAS
IVGKNAGELLLPFSQTITGKSSTFAMGSAIVSYPTRSEITKAAAFAAWEPTVFGAIPKKYAGFVAKVRRAFA

Sequences:

>Translated_472_residues
MKFTHDVIVIGGGAAGLTAAGGCALFGLKVALIEGHKMGGECLNNGCVPSKALITAAKRAAEARKQKRFGVELAAPNVEW
SGVHTHIHRAIAEIEPHDSAERFEEMGCEVIQDWARVTGKQSVEIGGRTLTAPRIVIATGSGPSVPPIPGLDAVPYLTNE
NIFDLEAQPDHLVIIGGGVIGMEMAQSFARLGSKVTVIEPGRPMGRDDEDSVAVVMEVMKKEGVTFVQGKAEKVEGRNGA
ITVHVDNGEQVSGSHLLIAVGRKARVSGFGLEDLGVELGNNGIKVDERRRTSVKNIYAIGDCREGPRLTHVSGYEGSNVA
LEITLGIPTKVDYKALPWCTYTEPEVAQIGLTEAEAKEKFGDKVTVVKEGFDHNERAITEGDTKGHMKVILKGKKVLGAS
IVGKNAGELLLPFSQTITGKSSTFAMGSAIVSYPTRSEITKAAAFAAWEPTVFGAIPKKYAGFVAKVRRAFA
>Mature_472_residues
MKFTHDVIVIGGGAAGLTAAGGCALFGLKVALIEGHKMGGECLNNGCVPSKALITAAKRAAEARKQKRFGVELAAPNVEW
SGVHTHIHRAIAEIEPHDSAERFEEMGCEVIQDWARVTGKQSVEIGGRTLTAPRIVIATGSGPSVPPIPGLDAVPYLTNE
NIFDLEAQPDHLVIIGGGVIGMEMAQSFARLGSKVTVIEPGRPMGRDDEDSVAVVMEVMKKEGVTFVQGKAEKVEGRNGA
ITVHVDNGEQVSGSHLLIAVGRKARVSGFGLEDLGVELGNNGIKVDERRRTSVKNIYAIGDCREGPRLTHVSGYEGSNVA
LEITLGIPTKVDYKALPWCTYTEPEVAQIGLTEAEAKEKFGDKVTVVKEGFDHNERAITEGDTKGHMKVILKGKKVLGAS
IVGKNAGELLLPFSQTITGKSSTFAMGSAIVSYPTRSEITKAAAFAAWEPTVFGAIPKKYAGFVAKVRRAFA

Specific function: Resistance to Hg(2+) in bacteria appears to be governed by a specialized system which includes mercuric reductase. MerA protein is responsible for volatilizing mercury as Hg(0) [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=476, Percent_Identity=32.1428571428571, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI50301238, Length=462, Percent_Identity=29.8701298701299, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI33519430, Length=427, Percent_Identity=26.463700234192, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519428, Length=427, Percent_Identity=26.463700234192, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI33519426, Length=427, Percent_Identity=26.463700234192, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=427, Percent_Identity=26.463700234192, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI148277071, Length=427, Percent_Identity=26.463700234192, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI22035672, Length=454, Percent_Identity=28.1938325991189, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI291045266, Length=474, Percent_Identity=25.3164556962025, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI291045268, Length=469, Percent_Identity=24.7334754797441, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1786307, Length=452, Percent_Identity=32.0796460176991, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789915, Length=427, Percent_Identity=29.9765807962529, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=28.6343612334802, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI87082354, Length=458, Percent_Identity=27.0742358078603, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789065, Length=193, Percent_Identity=26.4248704663212, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=31.5450643776824, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI71983429, Length=430, Percent_Identity=28.3720930232558, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=430, Percent_Identity=28.3720930232558, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=466, Percent_Identity=26.8240343347639, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=447, Percent_Identity=28.4116331096197, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=487, Percent_Identity=31.6221765913758, Blast_Score=166, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=28.3549783549784, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=22.964509394572, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI21358499, Length=464, Percent_Identity=32.3275862068966, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI24640553, Length=468, Percent_Identity=28.6324786324786, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24640549, Length=468, Percent_Identity=28.6324786324786, Blast_Score=148, Evalue=9e-36,
Organism=Drosophila melanogaster, GI24640551, Length=468, Percent_Identity=28.6324786324786, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17737741, Length=468, Percent_Identity=25.2136752136752, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000815
- InterPro:   IPR021179
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00403 HMA; PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.16.1.1 [H]

Molecular weight: Translated: 50132; Mature: 50132

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50006 FHA_DOMAIN ; PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTHDVIVIGGGAAGLTAAGGCALFGLKVALIEGHKMGGECLNNGCVPSKALITAAKRA
CCEEEEEEEECCCCCCCCCCCCHHHEEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHH
AEARKQKRFGVELAAPNVEWSGVHTHIHRAIAEIEPHDSAERFEEMGCEVIQDWARVTGK
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
QSVEIGGRTLTAPRIVIATGSGPSVPPIPGLDAVPYLTNENIFDLEAQPDHLVIIGGGVI
CEEEECCEEECCCEEEEEECCCCCCCCCCCCCCCCEECCCCEEEECCCCCEEEEECCCCH
GMEMAQSFARLGSKVTVIEPGRPMGRDDEDSVAVVMEVMKKEGVTFVQGKAEKVEGRNGA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCCE
ITVHVDNGEQVSGSHLLIAVGRKARVSGFGLEDLGVELGNNGIKVDERRRTSVKNIYAIG
EEEEECCCCEECCCEEEEEECCCCEECCCCHHHHHHEECCCCCEECHHHHCCCCEEEEEE
DCREGPRLTHVSGYEGSNVALEITLGIPTKVDYKALPWCTYTEPEVAQIGLTEAEAKEKF
CCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCHHEECCCHHHHHHHC
GDKVTVVKEGFDHNERAITEGDTKGHMKVILKGKKVLGASIVGKNAGELLLPFSQTITGK
CCEEEEEECCCCCCCCEECCCCCCCCEEEEEECCCEEEEEEECCCCCCEEEECHHHCCCC
SSTFAMGSAIVSYPTRSEITKAAAFAAWEPTVFGAIPKKYAGFVAKVRRAFA
CCCEECCCHHCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFTHDVIVIGGGAAGLTAAGGCALFGLKVALIEGHKMGGECLNNGCVPSKALITAAKRA
CCEEEEEEEECCCCCCCCCCCCHHHEEEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHH
AEARKQKRFGVELAAPNVEWSGVHTHIHRAIAEIEPHDSAERFEEMGCEVIQDWARVTGK
HHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
QSVEIGGRTLTAPRIVIATGSGPSVPPIPGLDAVPYLTNENIFDLEAQPDHLVIIGGGVI
CEEEECCEEECCCEEEEEECCCCCCCCCCCCCCCCEECCCCEEEECCCCCEEEEECCCCH
GMEMAQSFARLGSKVTVIEPGRPMGRDDEDSVAVVMEVMKKEGVTFVQGKAEKVEGRNGA
HHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCHHHCCCCCCE
ITVHVDNGEQVSGSHLLIAVGRKARVSGFGLEDLGVELGNNGIKVDERRRTSVKNIYAIG
EEEEECCCCEECCCEEEEEECCCCEECCCCHHHHHHEECCCCCEECHHHHCCCCEEEEEE
DCREGPRLTHVSGYEGSNVALEITLGIPTKVDYKALPWCTYTEPEVAQIGLTEAEAKEKF
CCCCCCCEEEECCCCCCCEEEEEEECCCCCCCCCCCCEEECCCCCHHEECCCHHHHHHHC
GDKVTVVKEGFDHNERAITEGDTKGHMKVILKGKKVLGASIVGKNAGELLLPFSQTITGK
CCEEEEEECCCCCCCCEECCCCCCCCEEEEEECCCEEEEEEECCCCCCEEEECHHHCCCC
SSTFAMGSAIVSYPTRSEITKAAAFAAWEPTVFGAIPKKYAGFVAKVRRAFA
CCCEECCCHHCCCCCHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2536669; 10559175; 2067577 [H]