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The map label for this gene is mfnA [H]

Identifier: 85372879

GI number: 85372879

Start: 71238

End: 72476

Strand: Direct

Name: mfnA [H]

Synonym: ELI_00260

Alternate gene names: 85372879

Gene position: 71238-72476 (Clockwise)

Preceding gene: 85372878

Following gene: 85372882

Centisome position: 2.33

GC content: 64.41

Gene sequence:

>1239_bases
ATGACGATGCCGAAACGCGGCCGGGACTGGGCCGAAGTGCGTGCCGAAATGATCGATCGCGGCGCCGGCGACGCCAAGTG
GCGCGATGGCAAGACGGCGGTGTATGTCTTCAATGCCGGTCCCGATATCGCCGAAGTCCAGCACGAAGCGTATGCGCTCT
ACATGTCCGAGAACGGCCTCGGTCCGCTCGCCTTTCCCTCGCTCGCGCAAATGGAGCGCGAGGTCATCGAAATGGCACTG
TCGCTGCTGCGCGGTCCCGAGGGTGCGGCAGGGGCGATGACTTCCGGCGGGACCGACAGCATCACAATGGCGGTCAAGGC
CGCGCGCGATTTCGCGCGGGCGGAAAAGGGCCTGAGCGGGCCCGCCAATATCGTCCTGCCGAAATCGGCGCACCCGGCCT
TCGACAAGGCAGCGCATCTGATGGACATCGAAGTGCGCCGCGTGCCGCTGAAAGACGACGGCAGTTACGAGGCCGATCCA
GCGGCGATGGATGCGGCTTGCGACGCAGCGACGATCATGATGGTCGGTTCGGCCCCCAACTTCCCGCACGGCATCGTCGA
CCCGATCATGACGCTCGGCGAAGTGGCGCAGGCGCGGGACATCTGGCTGCACACCGACGCCTGCGTCGGAGGCTATTTCG
CGCCGTTTGCGCGCATGAACGGTGTCGACGTTCCGCCCTTCGATTTCGAAGTGCCCGCCGTGCGTTCGATGAGCGCCGAC
CTGCACAAATACGGCTATGCCGCCAAGGGCGCGTCGACCGTGCTGTTCCGCTCGGAAGAGTATTACAATCATATGCCGTT
CGAGAACCGCGACTGGAGCGGCGCGCCGATGAAGACGCCGACCCTGGCCGGCACGCGGCCCGGCGGAGCCATTTCCGCGG
CTTGGGCTGTGATGCAGGTACTGGGCGTCGAAGGCTATCGCGAGAAACAGGGTCTCGTGTGCGCCACTCGCGAGCGCGTG
GAGGCTGGCGTGCGCGAGCTGGGCTTCGAAGTGCTGGGCAATCCGCTGCTCGGGCTGATTGCGTTCCGCCATCCCGAACA
CGACAATTACGCGATCTACAGCGAAATGTACCGCAAGGGCTGGTTCACCTCGCTGACCATCGAGCCGCCTGCTCTGCACC
TGATGCTTTCGCCCAAGCATGCCGAAGTCATCGACGATTACCTGGTGGATCTTGCTGCAGGCCTAGAAACTGTCGCGGCC
GGCAAGGAAGGGCCCCAGGTCGAAGCGCGTTACAACTGA

Upstream 100 bases:

>100_bases
ATATACGCGCATCGCCAACTTTCGCAGTTGGATCGAACGTGCCAAGGTTGCCGCGCGTGTTCCGGGCAAGCACCGGATTC
CCTGACGAAGTGAGATTGCG

Downstream 100 bases:

>100_bases
CACGTTGCAGCCCGAATATAAAAACGGGGCCGGATTGCTCCGGCCCCGCTGTAGTGTGCGCGTCGCGGAAGTGCTTACTG
CTCTTCGCGCGGACCGGTGA

Product: putative sphingosine-1-phosphate lyase

Products: NA

Alternate protein names: TDC [H]

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTMPKRGRDWAEVRAEMIDRGAGDAKWRDGKTAVYVFNAGPDIAEVQHEAYALYMSENGLGPLAFPSLAQMEREVIEMAL
SLLRGPEGAAGAMTSGGTDSITMAVKAARDFARAEKGLSGPANIVLPKSAHPAFDKAAHLMDIEVRRVPLKDDGSYEADP
AAMDAACDAATIMMVGSAPNFPHGIVDPIMTLGEVAQARDIWLHTDACVGGYFAPFARMNGVDVPPFDFEVPAVRSMSAD
LHKYGYAAKGASTVLFRSEEYYNHMPFENRDWSGAPMKTPTLAGTRPGGAISAAWAVMQVLGVEGYREKQGLVCATRERV
EAGVRELGFEVLGNPLLGLIAFRHPEHDNYAIYSEMYRKGWFTSLTIEPPALHLMLSPKHAEVIDDYLVDLAAGLETVAA
GKEGPQVEARYN

Sequences:

>Translated_412_residues
MTMPKRGRDWAEVRAEMIDRGAGDAKWRDGKTAVYVFNAGPDIAEVQHEAYALYMSENGLGPLAFPSLAQMEREVIEMAL
SLLRGPEGAAGAMTSGGTDSITMAVKAARDFARAEKGLSGPANIVLPKSAHPAFDKAAHLMDIEVRRVPLKDDGSYEADP
AAMDAACDAATIMMVGSAPNFPHGIVDPIMTLGEVAQARDIWLHTDACVGGYFAPFARMNGVDVPPFDFEVPAVRSMSAD
LHKYGYAAKGASTVLFRSEEYYNHMPFENRDWSGAPMKTPTLAGTRPGGAISAAWAVMQVLGVEGYREKQGLVCATRERV
EAGVRELGFEVLGNPLLGLIAFRHPEHDNYAIYSEMYRKGWFTSLTIEPPALHLMLSPKHAEVIDDYLVDLAAGLETVAA
GKEGPQVEARYN
>Mature_411_residues
TMPKRGRDWAEVRAEMIDRGAGDAKWRDGKTAVYVFNAGPDIAEVQHEAYALYMSENGLGPLAFPSLAQMEREVIEMALS
LLRGPEGAAGAMTSGGTDSITMAVKAARDFARAEKGLSGPANIVLPKSAHPAFDKAAHLMDIEVRRVPLKDDGSYEADPA
AMDAACDAATIMMVGSAPNFPHGIVDPIMTLGEVAQARDIWLHTDACVGGYFAPFARMNGVDVPPFDFEVPAVRSMSADL
HKYGYAAKGASTVLFRSEEYYNHMPFENRDWSGAPMKTPTLAGTRPGGAISAAWAVMQVLGVEGYREKQGLVCATRERVE
AGVRELGFEVLGNPLLGLIAFRHPEHDNYAIYSEMYRKGWFTSLTIEPPALHLMLSPKHAEVIDDYLVDLAAGLETVAAG
KEGPQVEARYN

Specific function: Specifically catalyzes the decarboxylation of L-tyrosine to produce tyramine [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Archaeal L-tyrosine decarboxylase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=400, Percent_Identity=37.25, Blast_Score=261, Evalue=1e-69,
Organism=Escherichia coli, GI1787769, Length=343, Percent_Identity=26.8221574344023, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789934, Length=343, Percent_Identity=26.8221574344023, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543922, Length=358, Percent_Identity=37.9888268156425, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17557272, Length=382, Percent_Identity=32.7225130890052, Blast_Score=207, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI25148342, Length=331, Percent_Identity=35.6495468277946, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6320500, Length=368, Percent_Identity=35.5978260869565, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6323905, Length=267, Percent_Identity=24.3445692883895, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21355963, Length=395, Percent_Identity=37.7215189873418, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24654344, Length=395, Percent_Identity=37.7215189873418, Blast_Score=279, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR020931 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.25 [H]

Molecular weight: Translated: 44624; Mature: 44493

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPKRGRDWAEVRAEMIDRGAGDAKWRDGKTAVYVFNAGPDIAEVQHEAYALYMSENGL
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHCCCEEEEEEECCCC
GPLAFPSLAQMEREVIEMALSLLRGPEGAAGAMTSGGTDSITMAVKAARDFARAEKGLSG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PANIVLPKSAHPAFDKAAHLMDIEVRRVPLKDDGSYEADPAAMDAACDAATIMMVGSAPN
CCCEEECCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCC
FPHGIVDPIMTLGEVAQARDIWLHTDACVGGYFAPFARMNGVDVPPFDFEVPAVRSMSAD
CCCHHHHHHHHHHHHHHHHHEEEECCHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LHKYGYAAKGASTVLFRSEEYYNHMPFENRDWSGAPMKTPTLAGTRPGGAISAAWAVMQV
HHHHCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LGVEGYREKQGLVCATRERVEAGVRELGFEVLGNPLLGLIAFRHPEHDNYAIYSEMYRKG
HCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCCHHHHHHHHCC
WFTSLTIEPPALHLMLSPKHAEVIDDYLVDLAAGLETVAAGKEGPQVEARYN
CCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC
>Mature Secondary Structure 
TMPKRGRDWAEVRAEMIDRGAGDAKWRDGKTAVYVFNAGPDIAEVQHEAYALYMSENGL
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHHHCCCEEEEEEECCCC
GPLAFPSLAQMEREVIEMALSLLRGPEGAAGAMTSGGTDSITMAVKAARDFARAEKGLSG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PANIVLPKSAHPAFDKAAHLMDIEVRRVPLKDDGSYEADPAAMDAACDAATIMMVGSAPN
CCCEEECCCCCCCHHHHHHEEEEEEEECCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCC
FPHGIVDPIMTLGEVAQARDIWLHTDACVGGYFAPFARMNGVDVPPFDFEVPAVRSMSAD
CCCHHHHHHHHHHHHHHHHHEEEECCHHHCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
LHKYGYAAKGASTVLFRSEEYYNHMPFENRDWSGAPMKTPTLAGTRPGGAISAAWAVMQV
HHHHCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LGVEGYREKQGLVCATRERVEAGVRELGFEVLGNPLLGLIAFRHPEHDNYAIYSEMYRKG
HCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCCCCHHHHHHHHCC
WFTSLTIEPPALHLMLSPKHAEVIDDYLVDLAAGLETVAAGKEGPQVEARYN
CCEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]