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The map label for this gene is trmE

Identifier: 85060406

GI number: 85060406

Start: 4166657

End: 4168021

Strand: Reverse

Name: trmE

Synonym: SG2428

Alternate gene names: 85060406

Gene position: 4168021-4166657 (Counterclockwise)

Preceding gene: 85060407

Following gene: 85060405

Centisome position: 99.93

GC content: 58.75

Gene sequence:

>1365_bases
ATGAGCCAGACGGATACCATTACTGCCCTTGCTACTCCGCCCGGACGCGGCAGCGTCGGCATATTGCGCGTCTCCGGCCC
CTTAGCCGCCCCGGTAGCCCGCGCGCTGCTGGGCAAGCTGCCGCGACCGCGTCAGGCTGAATATCTGCCCTTTCGCGACG
ACGACGGCACGACGCTTGATCAGGGCATCGCGCTGTTTTTTCCCGGCCCCCACTCCTTTACCGGCGAAGATGTCCTGGAA
TTGCAGGGCCATGGCGGCCCAGTCATATTGGATTTGTTGCTACAGCGTATCGTCGCGATGCCCGGCGTGCGCATTGCGCG
CCCCGGCGAGTTTTCGGAACGCGCCTTTCTTAACGACAAACTGGATTTGGCCCAGGCAGAGGCTATTGCCGATCTCATCG
ACGCCAGCTCGGCTCAGGCCGCACGCTCGGCGATCAATTCGCTACAGGGCGCGTTTTCCGGCCGTATTCATGAATTAGTG
AAAGCGCTTACTAATCTACGTATCTATGTCGAAGCGGCCATCGACTTTCCCGATGAAGAGATTGATTTCCTCTCCGAAGG
TAAAATTGAAGCCAGCCTGAACGACGTTATCGCCAGACTGGAACGGGTGCGCACCGAAGCGCGCCAGGGCAGTCTTCTGC
GCGAAGGGATGAAAGTGGTAATTGCCGGTAAGCCGAACGCCGGTAAATCCAGTTTGCTTAATGCGCTGGCGGGGCGCGAA
GCGGCTATCGTCACCGCCATTGCGGGCACGACACGCGACGTGCTGCGTGAACATATCCATCTCGACGGTATGCCGCTGCA
TATTATCGACACCGCCGGCCTGCGCAACGCCGGCGACGAGGTGGAGCGGATCGGCATTGAGCGCGCCTGGCGTGAAATTG
AACAAGCCGACCATGTGCTGCTGATGGTGGACGGCGCTGCCACCCCCCTCAGCGACCCGGATATGCTTTGGCCAGCGTTC
ATCGCCCGTTTGCCTTCCGGAATGCCGGTTACGGTAGTGCGCAATAAAGCCGACCTCACCGGCGAAAGCGTCGCTATTAG
TGATATAAGCGGTTACTTACTTATTACCCTATCCGCCCAGTCCGGTGTGGGCATGGACCTGCTGCGTACCCATTTGAAAC
AGAGCATGGGCTTTACCGGCGGCACCGAGGGGGGTTTTTTGGCCCGCAGGCGTCATCTCGACGCACTGGAGACTGCCGCG
ACCCATTTACAACAGGGAAAAGAACAGCTAGTAAGCGCTTGCTATGGAGAGTTGCTCGCTGAGGAGCTGCGTCTGGCGCA
GCAGTCTCTGAGCGAGATAACCGGCGAATTCAGCTCAGATGACCTGCTCGGCAGGATTTTTTCCAGTTTTTGCATTGGAA
AGTGA

Upstream 100 bases:

>100_bases
AGCGCATGCCCGGCCAGATAGCCACGCTGATAAGGCGGTCATCAATATGACCGCCTTTATTTTTTACAACCGCGCCCCGC
GGCGCAAAGCGAGCGTTTCA

Downstream 100 bases:

>100_bases
GTCCAGTTCCATCAACATCCTCACTATTCCGCTTTATCCCCAGCGGGCAAATTTCCCCCCTTCGCTGATTAAATCCATCC
TGCCTGTGACCGCATGATGG

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSQTDTITALATPPGRGSVGILRVSGPLAAPVARALLGKLPRPRQAEYLPFRDDDGTTLDQGIALFFPGPHSFTGEDVLE
LQGHGGPVILDLLLQRIVAMPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSAQAARSAINSLQGAFSGRIHELV
KALTNLRIYVEAAIDFPDEEIDFLSEGKIEASLNDVIARLERVRTEARQGSLLREGMKVVIAGKPNAGKSSLLNALAGRE
AAIVTAIAGTTRDVLREHIHLDGMPLHIIDTAGLRNAGDEVERIGIERAWREIEQADHVLLMVDGAATPLSDPDMLWPAF
IARLPSGMPVTVVRNKADLTGESVAISDISGYLLITLSAQSGVGMDLLRTHLKQSMGFTGGTEGGFLARRRHLDALETAA
THLQQGKEQLVSACYGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSQTDTITALATPPGRGSVGILRVSGPLAAPVARALLGKLPRPRQAEYLPFRDDDGTTLDQGIALFFPGPHSFTGEDVLE
LQGHGGPVILDLLLQRIVAMPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSAQAARSAINSLQGAFSGRIHELV
KALTNLRIYVEAAIDFPDEEIDFLSEGKIEASLNDVIARLERVRTEARQGSLLREGMKVVIAGKPNAGKSSLLNALAGRE
AAIVTAIAGTTRDVLREHIHLDGMPLHIIDTAGLRNAGDEVERIGIERAWREIEQADHVLLMVDGAATPLSDPDMLWPAF
IARLPSGMPVTVVRNKADLTGESVAISDISGYLLITLSAQSGVGMDLLRTHLKQSMGFTGGTEGGFLARRRHLDALETAA
THLQQGKEQLVSACYGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
>Mature_453_residues
SQTDTITALATPPGRGSVGILRVSGPLAAPVARALLGKLPRPRQAEYLPFRDDDGTTLDQGIALFFPGPHSFTGEDVLEL
QGHGGPVILDLLLQRIVAMPGVRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSAQAARSAINSLQGAFSGRIHELVK
ALTNLRIYVEAAIDFPDEEIDFLSEGKIEASLNDVIARLERVRTEARQGSLLREGMKVVIAGKPNAGKSSLLNALAGREA
AIVTAIAGTTRDVLREHIHLDGMPLHIIDTAGLRNAGDEVERIGIERAWREIEQADHVLLMVDGAATPLSDPDMLWPAFI
ARLPSGMPVTVVRNKADLTGESVAISDISGYLLITLSAQSGVGMDLLRTHLKQSMGFTGGTEGGFLARRRHLDALETAAT
HLQQGKEQLVSACYGELLAEELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=471, Percent_Identity=36.3057324840764, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI193082988, Length=503, Percent_Identity=34.1948310139165, Blast_Score=225, Evalue=8e-59,
Organism=Homo sapiens, GI193082993, Length=463, Percent_Identity=36.0691144708423, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=79.5154185022026, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=125, Percent_Identity=39.2, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=451, Percent_Identity=29.0465631929047, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323665, Length=505, Percent_Identity=29.9009900990099, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550813, Length=475, Percent_Identity=33.4736842105263, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SODGM (Q2NQ72)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_456108.1
- ProteinModelPortal:   Q2NQ72
- SMR:   Q2NQ72
- STRING:   Q2NQ72
- GeneID:   3866324
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2428
- NMPDR:   fig|343509.6.peg.5247
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q2NQ72
- ProtClustDB:   PRK05291
- BioCyc:   SGLO343509:SG2428-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 48669; Mature: 48538

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 80-80 BINDING 120-120 BINDING 454-454

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTDTITALATPPGRGSVGILRVSGPLAAPVARALLGKLPRPRQAEYLPFRDDDGTTLD
CCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
QGIALFFPGPHSFTGEDVLELQGHGGPVILDLLLQRIVAMPGVRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSAQAARSAINSLQGAFSGRIHELVKALTNLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHH
IDFLSEGKIEASLNDVIARLERVRTEARQGSLLREGMKVVIAGKPNAGKSSLLNALAGRE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTAIAGTTRDVLREHIHLDGMPLHIIDTAGLRNAGDEVERIGIERAWREIEQADHVL
CEEEEEHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LMVDGAATPLSDPDMLWPAFIARLPSGMPVTVVRNKADLTGESVAISDISGYLLITLSAQ
EEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEEECC
SGVGMDLLRTHLKQSMGFTGGTEGGFLARRRHLDALETAATHLQQGKEQLVSACYGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQTDTITALATPPGRGSVGILRVSGPLAAPVARALLGKLPRPRQAEYLPFRDDDGTTLD
CCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
QGIALFFPGPHSFTGEDVLELQGHGGPVILDLLLQRIVAMPGVRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCC
LDLAQAEAIADLIDASSAQAARSAINSLQGAFSGRIHELVKALTNLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHH
IDFLSEGKIEASLNDVIARLERVRTEARQGSLLREGMKVVIAGKPNAGKSSLLNALAGRE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTAIAGTTRDVLREHIHLDGMPLHIIDTAGLRNAGDEVERIGIERAWREIEQADHVL
CEEEEEHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEE
LMVDGAATPLSDPDMLWPAFIARLPSGMPVTVVRNKADLTGESVAISDISGYLLITLSAQ
EEECCCCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCCCEEEEEEECC
SGVGMDLLRTHLKQSMGFTGGTEGGFLARRRHLDALETAATHLQQGKEQLVSACYGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQSLSEITGEFSSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA