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The map label for this gene is trpS [H]

Identifier: 85060282

GI number: 85060282

Start: 3962539

End: 3963543

Strand: Reverse

Name: trpS [H]

Synonym: SG2304

Alternate gene names: 85060282

Gene position: 3963543-3962539 (Counterclockwise)

Preceding gene: 85060283

Following gene: 85060281

Centisome position: 95.02

GC content: 54.83

Gene sequence:

>1005_bases
ATGAGTAAGCCCATCGTATTCAGCGGCGCGCAGCCGTCAGGGGAACTGACTATTGGCAACTATATGGGTGCGCTGCGTCA
GTGGGTCAGCATGCAGGACGAGTATGACTGTATTTATTGCATCGTCGACCTACATGCCATTACCGCCCGTCAGGATGCCC
AGCAGCTGCGTAAGGCAACGCTGGACGTTCTGGCGCTGTATCTGGCCTGCGGTATCGATCCGGCTAAAAGTACGGTTTTC
ATTCAGTCGCATGTGCCGGAGCATAGCCAACTGGGCTGGTTGCTGAACTGTTATACCTATTTTGGTGAACTGAGCCGGAT
GACCCAGTTCAAGGATAAATCGGCCCGTTACGCCGAAAATATCAATGCCGGGCTGTTTGATTACCCGGTGCTGATGGCGG
CAGACATTCTGCTTTATCAGACCAACCAGGTGCCGGTCGGGGAGGATCAGAAACAGCATTTGGAGCTGAGCCGCGATATC
GCCAAACGGTTTAACGCGCTGTATGGCGAGATCTTCACCATTCCTGAACCGTTTATTCCCAAATCCGGCGCGCGCGTGAT
GTCGTTGCTGGATCCTTTGAAAAAGATGTCGAAATCCGATGACAACCGTAATAACGTCATCACCCTGCTGGAAGATCCCA
AATCCGTAGTCAAGAAGCTTAAACGGGCGGTAACCGACTCCGATGAACCGCCGGTGGTGCGCTATGATCCTGTGGCCAAA
CCCGGCGTCTCCAACTTGCTGGATATTTTATCCGGCGTGACCGGTACGCCGGTGGCGGAACTGGAACAGGCGTTTGCCGG
AAAAATGTACGGCCATCTGAAATCCGCCGTGGCCGATGCGGTGTCGGAAATGCTGACCGCGTTACAGGCTCGTTACCACG
CCCTGTGCGCCGACGAAGCTTATTTGAATCAAGTGTTACGGGACGGGGCGGAAAAAGCCCGCGCTCAAGCCAGCGTGACC
TTGGCCAAGGTCTATGAAGCCGTCGGCTTTGTCGCCAAACCCTGA

Upstream 100 bases:

>100_bases
AAGCGATCACGCTGAGCCGCCCGGATTACGTTCTGGACCGTTTTGCCGATCTTTTGCCCGTGCTCGGGCTGCCACCTTTC
GTTAATCAGGGCCGTTTAAA

Downstream 100 bases:

>100_bases
GTCATTTATACCCCTCGCGCGCCCGGCCTGATCCCGATAGGGCCGGGCGGCACGTCACTACGCTTTTTCCGCGACTCCCG
GCAAAGGTAATGAATTATAT

Product: tryptophanyl-tRNA synthase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MSKPIVFSGAQPSGELTIGNYMGALRQWVSMQDEYDCIYCIVDLHAITARQDAQQLRKATLDVLALYLACGIDPAKSTVF
IQSHVPEHSQLGWLLNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDI
AKRFNALYGEIFTIPEPFIPKSGARVMSLLDPLKKMSKSDDNRNNVITLLEDPKSVVKKLKRAVTDSDEPPVVRYDPVAK
PGVSNLLDILSGVTGTPVAELEQAFAGKMYGHLKSAVADAVSEMLTALQARYHALCADEAYLNQVLRDGAEKARAQASVT
LAKVYEAVGFVAKP

Sequences:

>Translated_334_residues
MSKPIVFSGAQPSGELTIGNYMGALRQWVSMQDEYDCIYCIVDLHAITARQDAQQLRKATLDVLALYLACGIDPAKSTVF
IQSHVPEHSQLGWLLNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDI
AKRFNALYGEIFTIPEPFIPKSGARVMSLLDPLKKMSKSDDNRNNVITLLEDPKSVVKKLKRAVTDSDEPPVVRYDPVAK
PGVSNLLDILSGVTGTPVAELEQAFAGKMYGHLKSAVADAVSEMLTALQARYHALCADEAYLNQVLRDGAEKARAQASVT
LAKVYEAVGFVAKP
>Mature_333_residues
SKPIVFSGAQPSGELTIGNYMGALRQWVSMQDEYDCIYCIVDLHAITARQDAQQLRKATLDVLALYLACGIDPAKSTVFI
QSHVPEHSQLGWLLNCYTYFGELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDIA
KRFNALYGEIFTIPEPFIPKSGARVMSLLDPLKKMSKSDDNRNNVITLLEDPKSVVKKLKRAVTDSDEPPVVRYDPVAKP
GVSNLLDILSGVTGTPVAELEQAFAGKMYGHLKSAVADAVSEMLTALQARYHALCADEAYLNQVLRDGAEKARAQASVTL
AKVYEAVGFVAKP

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=331, Percent_Identity=45.0151057401813, Blast_Score=284, Evalue=1e-76,
Organism=Homo sapiens, GI41352700, Length=179, Percent_Identity=52.5139664804469, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI1789786, Length=334, Percent_Identity=84.4311377245509, Blast_Score=592, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI71982800, Length=334, Percent_Identity=35.9281437125749, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71982793, Length=334, Percent_Identity=35.9281437125749, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6320474, Length=349, Percent_Identity=40.1146131805158, Blast_Score=246, Evalue=4e-66,
Organism=Drosophila melanogaster, GI24666151, Length=329, Percent_Identity=42.5531914893617, Blast_Score=252, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36868; Mature: 36736

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPIVFSGAQPSGELTIGNYMGALRQWVSMQDEYDCIYCIVDLHAITARQDAQQLRKAT
CCCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
LDVLALYLACGIDPAKSTVFIQSHVPEHSQLGWLLNCYTYFGELSRMTQFKDKSARYAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDIAKRFNALYGEIFTIPEPFIP
CCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KSGARVMSLLDPLKKMSKSDDNRNNVITLLEDPKSVVKKLKRAVTDSDEPPVVRYDPVAK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCC
PGVSNLLDILSGVTGTPVAELEQAFAGKMYGHLKSAVADAVSEMLTALQARYHALCADEA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YLNQVLRDGAEKARAQASVTLAKVYEAVGFVAKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKPIVFSGAQPSGELTIGNYMGALRQWVSMQDEYDCIYCIVDLHAITARQDAQQLRKAT
CCCEEEECCCCCCCEEHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHH
LDVLALYLACGIDPAKSTVFIQSHVPEHSQLGWLLNCYTYFGELSRMTQFKDKSARYAEN
HHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
INAGLFDYPVLMAADILLYQTNQVPVGEDQKQHLELSRDIAKRFNALYGEIFTIPEPFIP
CCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KSGARVMSLLDPLKKMSKSDDNRNNVITLLEDPKSVVKKLKRAVTDSDEPPVVRYDPVAK
CCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCEEEECCCCC
PGVSNLLDILSGVTGTPVAELEQAFAGKMYGHLKSAVADAVSEMLTALQARYHALCADEA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
YLNQVLRDGAEKARAQASVTLAKVYEAVGFVAKP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]