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The map label for this gene is tusD

Identifier: 85060267

GI number: 85060267

Start: 3940236

End: 3940628

Strand: Reverse

Name: tusD

Synonym: SG2289

Alternate gene names: 85060267

Gene position: 3940628-3940236 (Counterclockwise)

Preceding gene: 85060268

Following gene: 85060266

Centisome position: 94.47

GC content: 65.39

Gene sequence:

>393_bases
ATGAGCCTGGACTATTGCCTGATGGTCACCGGGCCGGCCTATGGCAATCAGCGCGCCAGCAGCGCGCTACAGTTCGCCCG
GGCGCTGCTGGAGTGCGGCCACCGTCTCGGCACGGTGTTTTTTTACCAGGACGGCGTACATAACGCCAACCGCTTGAGTG
CGCCCGCCGGCGATGAGGTGGATCTGGTGCGCGCCTGGCACGAGCTGGCGCAGCAGCATCAGGTGGCGCTGCATGTCTGC
GCCGCCGCCGCCCTGCGTCGTGGCGTAACCGATGCCCTTCAGGCGTCGCTGCTTAAGCGTAGCGGCGAAAATCTTCAGCC
CGGCTTTGAGCTCAGCGGCCTGGGCAGCCTGGTACAGGCGGCGCTCGGCTGCGATCGCTTTATCCAGTTCTAA

Upstream 100 bases:

>100_bases
ACGCCATCAACCAGGTTGCCGACAGGCTCAACATCTCCAAGCACACGGTTTACTTGTATATCCGTCAGTTTAAGAGCGGT
GATTTCGTGGGGCAGGAGCG

Downstream 100 bases:

>100_bases
GGCTTCCATGAACCGTATCGCATTTGTTTTCACCCACGGTCCGCACGGCGACGCCGCTGGACGGGAAGGGCTTGACGCGC
TGTTGGCGACCTCGGCATTG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein D

Number of amino acids: Translated: 130; Mature: 129

Protein sequence:

>130_residues
MSLDYCLMVTGPAYGNQRASSALQFARALLECGHRLGTVFFYQDGVHNANRLSAPAGDEVDLVRAWHELAQQHQVALHVC
AAAALRRGVTDALQASLLKRSGENLQPGFELSGLGSLVQAALGCDRFIQF

Sequences:

>Translated_130_residues
MSLDYCLMVTGPAYGNQRASSALQFARALLECGHRLGTVFFYQDGVHNANRLSAPAGDEVDLVRAWHELAQQHQVALHVC
AAAALRRGVTDALQASLLKRSGENLQPGFELSGLGSLVQAALGCDRFIQF
>Mature_129_residues
SLDYCLMVTGPAYGNQRASSALQFARALLECGHRLGTVFFYQDGVHNANRLSAPAGDEVDLVRAWHELAQQHQVALHVCA
AAALRRGVTDALQASLLKRSGENLQPGFELSGLGSLVQAALGCDRFIQF

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Accepts sulfur from tusA and transfers it in turn to tusE

COG id: COG1553

COG function: function code P; Uncharacterized conserved protein involved in intracellular sulfur reduction

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrE/tusD family

Homologues:

Organism=Escherichia coli, GI1789743, Length=128, Percent_Identity=59.375, Blast_Score=162, Evalue=8e-42,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): TUSD_SODGM (Q2NQL1)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455969.1
- ProteinModelPortal:   Q2NQL1
- SMR:   Q2NQL1
- STRING:   Q2NQL1
- GeneID:   3866431
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2289
- NMPDR:   fig|343509.6.peg.4977
- eggNOG:   COG1553
- HOGENOM:   HBG538485
- OMA:   QDGVYNS
- PhylomeDB:   Q2NQL1
- BioCyc:   SGLO343509:SG2289-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00390
- InterPro:   IPR003787
- InterPro:   IPR017463
- TIGRFAMs:   TIGR03012

Pfam domain/function: PF02635 DrsE

EC number: NA

Molecular weight: Translated: 14027; Mature: 13896

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: ACT_SITE 80-80

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDYCLMVTGPAYGNQRASSALQFARALLECGHRLGTVFFYQDGVHNANRLSAPAGDEV
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHH
DLVRAWHELAQQHQVALHVCAAAALRRGVTDALQASLLKRSGENLQPGFELSGLGSLVQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALGCDRFIQF
HHHHHHHHCC
>Mature Secondary Structure 
SLDYCLMVTGPAYGNQRASSALQFARALLECGHRLGTVFFYQDGVHNANRLSAPAGDEV
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCHH
DLVRAWHELAQQHQVALHVCAAAALRRGVTDALQASLLKRSGENLQPGFELSGLGSLVQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
ALGCDRFIQF
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA