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The map label for this gene is tusB

Identifier: 85060265

GI number: 85060265

Start: 3939567

End: 3939854

Strand: Reverse

Name: tusB

Synonym: SG2287

Alternate gene names: 85060265

Gene position: 3939854-3939567 (Counterclockwise)

Preceding gene: 85060266

Following gene: 85060264

Centisome position: 94.45

GC content: 60.76

Gene sequence:

>288_bases
ATGCTGTATACCCTGAGCCGTTCTCCCTATGCCTGCGATCTGGCGGCGTTGCTGCGTATCGCGCAGCACGGCGACGACCT
GCTGCTGCTGTCCGATGGCGTTATCGCCGGGCTCACCGGCTCACCGGCCGCGTGCGCGCTCGGTGCCTCTCCCCTGACGC
TGCACGCGCTGGAAAACGATATTGTCGCTCGCGGTCTGTCTGCTCATCTTTCGCCCAACATCGCGATAATCAGTTATACT
GATTTCGTCAGGCTGACCGAAAAACAGCCGCAGCAGATGGCGTGGTAA

Upstream 100 bases:

>100_bases
CTGGCTGGGTATTGAACGCTGAATGGTTGTCGGCGGCGGACTGGCGCCAGCGTCTTAGCGCCTATGATACCGTGATGACG
TTTTAACGCCGGAGGCGCCC

Downstream 100 bases:

>100_bases
GCAAGGGTGAGTTGTATATTTCTTGACACCTTTACCCGTCAGCCATAAAATTCTGCGTCCTCGTACTTTGCCCCGCAGCA
TCACGAGGCGATTTATTACG

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLYTLSRSPYACDLAALLRIAQHGDDLLLLSDGVIAGLTGSPAACALGASPLTLHALENDIVARGLSAHLSPNIAIISYT
DFVRLTEKQPQQMAW

Sequences:

>Translated_95_residues
MLYTLSRSPYACDLAALLRIAQHGDDLLLLSDGVIAGLTGSPAACALGASPLTLHALENDIVARGLSAHLSPNIAIISYT
DFVRLTEKQPQQMAW
>Mature_95_residues
MLYTLSRSPYACDLAALLRIAQHGDDLLLLSDGVIAGLTGSPAACALGASPLTLHALENDIVARGLSAHLSPNIAIISYT
DFVRLTEKQPQQMAW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=52.6315789473684, Blast_Score=105, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TUSB_SODGM (Q2NQL3)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455967.1
- ProteinModelPortal:   Q2NQL3
- SMR:   Q2NQL3
- STRING:   Q2NQL3
- GeneID:   3866429
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG2287
- NMPDR:   fig|343509.6.peg.4975
- eggNOG:   COG2168
- HOGENOM:   HBG682569
- OMA:   SPWHADI
- PhylomeDB:   Q2NQL3
- ProtClustDB:   PRK13510
- BioCyc:   SGLO343509:SG2287-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01564
- InterPro:   IPR007215
- TIGRFAMs:   TIGR03011

Pfam domain/function: PF04077 DsrH

EC number: NA

Molecular weight: Translated: 10089; Mature: 10089

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYTLSRSPYACDLAALLRIAQHGDDLLLLSDGVIAGLTGSPAACALGASPLTLHALEND
CEEECCCCCCEECHHHHHHHHHCCCCEEEEECCEEECCCCCCCEEEECCCCEEEEEHHCH
IVARGLSAHLSPNIAIISYTDFVRLTEKQPQQMAW
HHHCCCCCCCCCCEEEEEECCEEEECCCCCCCCCC
>Mature Secondary Structure
MLYTLSRSPYACDLAALLRIAQHGDDLLLLSDGVIAGLTGSPAACALGASPLTLHALEND
CEEECCCCCCEECHHHHHHHHHCCCCEEEEECCEEECCCCCCCEEEECCCCEEEEEHHCH
IVARGLSAHLSPNIAIISYTDFVRLTEKQPQQMAW
HHHCCCCCCCCCCEEEEEECCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA